Sequence-specific detection method for reverse transcription, loop-mediated isothermal amplification of HIV-1.

abstract：:SARS-CoV-2 infection is causing a pandemic disease that is reflected in challenging public health problems worldwide. Human leukocyte antigen (HLA)-based epitope prediction and its association with disease outcomes provide an important base for treatment design. A bioinformatic prediction of T cell epitopes and their ...

journal_title：Journal of medical virology

authors： Romero-López JP,Carnalla-Cortés M,Pacheco-Olvera DL,Ocampo-Godínez JM,Oliva-Ramírez J,Moreno-Manjón J,Bernal-Alferes B,López-Olmedo N,García-Latorre E,Domínguez-López ML,Reyes-Sandoval A,Jiménez-Zamudio L

更新日期：2020-09-28 00:00:00

abstract：:The avalanche of genomic data generated from the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus requires the development of tools to detect and monitor its mutations across the world. Here, we present a webtool, coronapp, dedicated to easily processing user-provided SARS-CoV-2 genomic sequences and...

journal_title：Journal of medical virology

authors： Mercatelli D,Triboli L,Fornasari E,Ray F,Giorgi FM

更新日期：2020-11-18 00:00:00

abstract：:During the 1998-1999 influenza season, two distinct influenza B virus Yamagata group strains were isolated from the patients of a private clinic. Each responded differently to monoclonal antibodies (Mabs) 5H4 and 8B3 on staining, and hemagglutination inhibition and neutralizing tests. When the analysis of nucleotide s...

journal_title：Journal of medical virology

authors： Nakagawa N,Kubota R,Morikawa S,Nakagawa T,Baba K,Okuno Y

更新日期：2001-12-01 00:00:00

abstract：:A reverse transcription-polymerase chain reaction (RT-PCR) was developed for the detection of Chikungunya virus infection. Based on the nonstructural protein 1 (nsP1) and glycoprotein E1 (E1) genes of Chikungunya, two primer sets were designed. Total RNA were extracted from the cell culture fluid of Aedes albopictus C...

journal_title：Journal of medical virology

authors： Hasebe F,Parquet MC,Pandey BD,Mathenge EG,Morita K,Balasubramaniam V,Saat Z,Yusop A,Sinniah M,Natkunam S,Igarashi A

更新日期：2002-07-01 00:00:00

abstract：:Herpes simplex virus can be quantitatively recovered from vaginal tampons suspended in tissue culture medium and stored over a wide range of temperatures. Because herpes simplex virus is stable for several days with refrigeration or after freezing, this method of culture of cervicovaginal virus lends itself to epidemi...

journal_title：Journal of medical virology

authors： Myers MG,Ekwo EE,Pitts AK

更新日期：1979-01-01 00:00:00

abstract：:We have previously reported that concanavalin A-immobilized polystyrene nanospheres (Con A-NS) could efficiently capture HIV-1 particles and that intranasal immunization with inactivated HIV-1-capturing nanospheres (HIV-NS) induced vaginal anti-HIV-1 IgA antibody response in mice. In this study, to evaluate the protec...

journal_title：Journal of medical virology

authors： Miyake A,Akagi T,Enose Y,Ueno M,Kawamura M,Horiuchi R,Hiraishi K,Adachi M,Serizawa T,Narayan O,Akashi M,Baba M,Hayami M

更新日期：2004-07-01 00:00:00

abstract：:The aim of the study was to assess the prevalence of human papillomavirus (HPV) in the esophagus in the coastal region of Eastern Guangdong, Southern China, an area with a high incidence of esophageal carcinoma. Fresh surgical resection esophageal specimens were obtained from 176 esophageal carcinoma patients admitted...

journal_title：Journal of medical virology

authors： Shen ZY,Hu SP,Lu LC,Tang CZ,Kuang ZS,Zhong SP,Zeng Y

更新日期：2002-11-01 00:00:00

abstract：:A metagenomic approach based on target independent next-generation sequencing has become a known method for the detection of both known and novel viruses in clinical samples. This study aimed to use the metagenomic sequencing approach to characterize the viral diversity in respiratory samples from patients with respir...

journal_title：Journal of medical virology

authors： Madi N,Al-Nakib W,Mustafa AS,Habibi N

更新日期：2018-03-01 00:00:00

abstract：:Although hepatitis E virus (HEV) infection is a zoonosis, there is, currently, no standardized assay for quantitatively measuring anti-HEV antibody levels in HEV animal reservoirs. In this study, anti-HEV antibody positive serum from a rabbit (RS26) was calibrated by dose-response parallel line assay using the World H...

journal_title：Journal of medical virology

authors： Zhang Y,Wang L,Zeng H,Gong W,Wang L,Zhuang H

更新日期：2017-03-01 00:00:00

abstract：:The resurgence of Chikungunya virus (CHIKV) in the form of unprecedented and explosive epidemics in India and the Indian Ocean islands after a gap of 32 years is a major public health concern. Currently, there is no specific therapy available to treat CHIKV infection. In the present study, the in vitro prophylactic an...

journal_title：Journal of medical virology

authors： Khan M,Santhosh SR,Tiwari M,Lakshmana Rao PV,Parida M

更新日期：2010-05-01 00:00:00

abstract：:Described for the first time in 1986, Parvovirus B19 (B19V) infection in kidney transplant recipients remains little-known and probably underestimated. The aims of this study were to establish B19V infection frequency during the first year after kidney transplant and to determine predisposing factors and manifestation...

journal_title：Journal of medical virology

authors： Porignaux R,Vuiblet V,Barbe C,Nguyen Y,Lavaud S,Toupance O,Andréoletti L,Rieu P,Lévêque N

更新日期：2013-06-01 00:00:00

abstract：:Human herpesvirus 6 (HHV6) is a beta-herpesvirus capable of infecting several cell types from different origins. HHV6 is the etiological agent of exantem subitum and has been associated with several diseases, all characterized by an inflammatory response triggered by chemokines. We show that strain U1102 of HHV6 is ab...

journal_title：Journal of medical virology

authors： Caruso A,Favilli F,Rotola A,Comar M,Horejsh D,Alessandri G,Grassi M,Di Luca D,Fiorentini S

更新日期：2003-07-01 00:00:00

abstract：:The prevalence rate of IgG antibodies to cytomegalovirus (CMV) was determined in a sample of 567 women of childbearing age in the southern part of Israel by the immunoperoxidase assay to membrane antigen (IPAMA) technique. Urban Jewish women of Afro-Asian origin showed significantly higher rates of seropositivity than...

journal_title：Journal of medical virology

authors： Urkin J,Sarov B,Naggan L,Haikin H,Sarov I

更新日期：1988-01-01 00:00:00

abstract：:An outbreak of an acute sporadic viral hepatitis occurring at three villages in the lower Shebli region of southern Somalia was studied. Sera were examined for antibodies to hepatitis E virus (HEV) antigen by a recently developed enzyme-linked immunosorbent assay. The prevalence of anti-HEV ranged from 77.8% to 94.0% ...

journal_title：Journal of medical virology

authors： Mushahwar IK,Dawson GJ,Bile KM,Magnius LO

更新日期：1993-07-01 00:00:00

abstract：:Enterovirus (EV) infections are common. There are more than 60 known serotypes, and each has different epidemiologic or medical importance. Over 700 physicians from 75% of basic administrative units of Taiwan participated in the "Sentinel Physician Surveillance of Infectious Disease" and reported weekly to the Center ...

journal_title：Journal of medical virology

authors： Tseng FC,Huang HC,Chi CY,Lin TL,Liu CC,Jian JW,Hsu LC,Wu HS,Yang JY,Chang YW,Wang HC,Hsu YW,Su IJ,Wang JR,CDC-Taiwan Virology Reference Laboratories and Sentinel Physician Network.

更新日期：2007-12-01 00:00:00

abstract：:The predictive value of two methods for measuring HIV RNA concentration in plasma was assessed in relation to CD4 lymphocyte counts during the asymptomatic period of infection. The design was a retrospective longitudinal case-control study for a mean period of 60 months involving 20 asymptomatic patients included in t...

journal_title：Journal of medical virology

authors： Delamare C,Burgard M,Deveau C,Chaix ML,Ivanoff S,Ramirez D,Tachet A,Bary M,Rouzioux C

更新日期：1998-01-01 00:00:00

abstract：:Noroviruses are an important cause of acute gastroenteritis. Increasing data showed that the GII-4 strains are predominant worldwide and new GII-4 variants emerge every 1-2 years causing major epidemics. Surveillance of gastroenteritis in hospitalized children under 5 years of age in China is described. Among 1,110 sp...

journal_title：Journal of medical virology

authors： Jin M,Xie HP,Duan ZJ,Liu N,Zhang Q,Wu BS,Li HY,Cheng WX,Yang SH,Yu JM,Xu ZQ,Cui SX,Zhu L,Tan M,Jiang X,Fang ZY

更新日期：2008-11-01 00:00:00

abstract：:The relative frequency and distribution of the VP4 (P) genotypes of 227 human rotavirus field strains were investigated in South Africa. The stool samples were collected between 1984-1993 from infants and young children with diarrhea at Ga-Rankuwa Hospital, Pretoria, South Africa. The RNA was extracted from stools, he...

journal_title：Journal of medical virology

authors： Mphahlele MJ,Steele AD

更新日期：1995-09-01 00:00:00

abstract：:Between 1999 and 2007 1,388 stool specimens from patients with acute flaccid paralysis or aseptic meningitis were submitted to the Austrian reference laboratory for poliomyelitis. Samples (201) yielded non-poliovirus enterovirus in culture. One hundred eighty-one viruses were available for typing and 78 isolates which...

journal_title：Journal of medical virology

authors： Ortner B,Huang CW,Schmid D,Mutz I,Wewalka G,Allerberger F,Yang JY,Huemer HP

更新日期：2009-02-01 00:00:00

abstract：:At present eight hepatitis B virus (HBV) genotypes have been characterized: A to H. The most common genotype in Northern Europe is genotype A. So far there is no record of the specific HBV genotype distribution in Iceland. Iceland has a small population whose homogeneity has changed due to increasing migration during ...

journal_title：Journal of medical virology

authors： Björnsdottir TB,Stanzeit B,Sällberg M,Löve A,Hultgren C

更新日期：2005-12-01 00:00:00

abstract：:Throughout most of the last decade, B/Yamagata/16/88-lineage influenza viruses were predominant among the B isolates circulating worldwide, whereas B/Victoria/2/87-lineage viruses were isolated infrequently and restricted geographically to eastern Asia. During the 2001-02 influenza season, B/Victoria/2/87-lineage viru...

journal_title：Journal of medical virology

authors： Puzelli S,Frezza F,Fabiani C,Ansaldi F,Campitelli L,Lin YP,Gregory V,Bennett M,D'Agaro P,Campello C,Crovari P,Hay A,Donatelli I

更新日期：2004-12-01 00:00:00

abstract：:Three hepatitis B surface antigen (HBsAg) preparations were compared: purified intact 22-nm HBsAg particles; HBsAg-derived, sodium dodecyl sulfate (SDS)-denatured P25 + GP30 polypeptide pool; and nondenatured P25 + GP30 micelles. The micelles had the same polypeptide composition as the P25 + GP30 pool. The immunogenic...

journal_title：Journal of medical virology

authors： Sanchez Y,Ionescu-Matiu I,Melnick JL,Dreesman GR

更新日期：1983-01-01 00:00:00

abstract：:The presence of antibody to hepatitis A virus (anti-HAV) in 60% of procured owl monkeys (Aotus trivirgatus) held within the United States prompted a study of recently captured A trivirgatus in Panama. Only 2 of 145 newly captured monkeys, but all of 35 A trivirgatus held within a colony for over 100 days, were found t...

journal_title：Journal of medical virology

authors： Lemon SM,LeDuc JW,Binn LN,Escajadillo A,Ishak KG

更新日期：1982-01-01 00:00:00

abstract：:Serum levels of interleukin-6 (IL-6) are elevated in acute and chronic hepatitis B patients. The effect of IL-6 and its transcription factor of NF-IL6 (a nuclear factor for IL-6) on hepatitis B virus (HBV) enhancer 1 (Enh1), which controls HBV X expression, were investigated in HepG2 cells. Twenty ng/ml of IL-6 increa...

journal_title：Journal of medical virology

authors： Ohno H,Kaneko S,Kobayashi K,Murakami S

更新日期：1997-08-01 00:00:00

abstract：:The replication strategy of a human serotype 1 rotavirus, adapted to rapid growth in CV-1 cells, was investigated. A single cycle growth curve revealed eclipse and latent periods of 3 and 4 hours, respectively. Although the extent of reduction of host cell protein synthesis was directly related to the multiplicity of ...

journal_title：Journal of medical virology

authors： Heath RL,Birch CJ

更新日期：1988-05-01 00:00:00

abstract：:The effect of antiviral treatment on apoptosis in chronic hepatitis B (CHB) has not been clarified. We evaluated the hepatic immunohistochemical expression of the pro-apoptotic bax and the antiapoptotic bcl-xL protein in HBeAg-negative CHB patients before and after treatment. In our study we included 72 paired biopsie...

journal_title：Journal of medical virology

authors： Cholongitas E,Papatheodoridis GV,Manesis EK,Petraki K,Tiniakos D,Hadziyannis SJ

更新日期：2011-07-01 00:00:00

abstract：:Recently, human platelet antigens (HPAs) polymorphisms are found to play a role in susceptibility to hepatitis C virus (HCV) infection and fibrosis progression. The aim of the current study was to evaluate the possible association between the HPAs polymorphisms with liver fibrosis progression in HCV patients. HPAs pol...

journal_title：Journal of medical virology

authors： Ghasemi A,Zadsar M,Shaiegan M,Samiei S,Namvar A,Rasouli M,Moosanejad M

更新日期：2020-01-01 00:00:00

abstract：:Kaposi's sarcoma-associated herpesvirus (KSHV), the etiologic agent of Kaposi's sarcoma, causes malignancies frequently in patients with acquired immunodeficiency syndrome. In the United States and Europe, KSHV infection is common among men who have sex with men. However, the seroprevalence of KSHV among men who have ...

journal_title：Journal of medical virology

authors： Katano H,Yokomaku Y,Fukumoto H,Kanno T,Nakayama T,Shingae A,Sugiura W,Ichikawa S,Yasuoka A

更新日期：2013-06-01 00:00:00

abstract：:Natural history of anal intraepithelial neoplasia and anal cancer is not fully understood. Factors associated with cytological abnormalities and predictors of progression to high-grade anal intraepithelial neoplasia still deserve investigation. The aim of this cross-sectional study was to assess the prevalence of HPV ...

journal_title：Journal of medical virology

authors： Tamalet C,Obry-Roguet V,Ressiot E,Bregigeon S,Del Grande J,Poizot-Martin I

更新日期：2014-03-01 00:00:00

abstract：:Chikungunya fever a re-emerging infection with expanding geographical boundaries, can mimic symptoms of other infections like dengue, malaria which makes the definitive diagnosis of the infection important. The present study compares the utility of four laboratory diagnostic methods viz. IgM capture ELISA, an in house...

journal_title：Journal of medical virology

authors： Reddy V,Ravi V,Desai A,Parida M,Powers AM,Johnson BW

更新日期：2012-11-01 00:00:00