Abstract:
:Although hepatitis E virus (HEV) infection is a zoonosis, there is, currently, no standardized assay for quantitatively measuring anti-HEV antibody levels in HEV animal reservoirs. In this study, anti-HEV antibody positive serum from a rabbit (RS26) was calibrated by dose-response parallel line assay using the World Health Organization (WHO) reference standard for anti-HEV antibodies. After evaluating the stability of the RS26 serum, a quantification assay of anti-HEV antibodies using RS26 as a standard was developed and evaluated for both reproducibility and suitability to field studies. The anti-HEV antibody level of RS26 was calculated to be 39.54 IU/ml. A series of standard working serum for anti-HEV antibodies consisted of five serum dilutions (3.950 IU/ml, 1.975 IU/ml, 0.986 IU/ml, 0.494 IU/ml, and 0.247 IU/ml). The quantitative assay using RS26 showed good range and reproducibility, effectively measuring the dynamics of anti-HEV antibody concentrations in pigs and rabbits. In conclusion, we have developed a a stable and reproducible serum standard for the quantitation of anti-HEV antibodies. J. Med. Virol. 89:497-501, 2017. © 2016 Wiley Periodicals, Inc.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Zhang Y,Wang L,Zeng H,Gong W,Wang L,Zhuang Hdoi
10.1002/jmv.24651subject
Has Abstractpub_date
2017-03-01 00:00:00pages
497-501issue
3eissn
0146-6615issn
1096-9071journal_volume
89pub_type
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