Nested polymerase chain reaction for detection of human immunodeficiency virus type 1 DNA in clinical specimens.

abstract：:Monoclonal antibodies were raised against hepatitis B core antigen (HBcAg) synthesized in Escherichia coli. They identified a single immunodominant determinant on both liver-derived and E. coli-derived HBcAg. Cross-inhibition studies demonstrated that HBsAg-containing human sera which contained antibodies to HBcAg (an...

journal_title：Journal of medical virology

authors： Ferns RB,Tedder RS

更新日期：1986-06-01 00:00:00

abstract：:Cytomegalovirus-specific complement-fixing IgM (CMV CF-IgM) antibody was detected in 12/84 (14.3 percent) sera containing CMV-specific IgM. Higher titres were obtained by using a purified antigen preparation containing enveloped virus particles and membrane fragments when compared with a crude extract of infected cell...

journal_title：Journal of medical virology

authors： Booth JC,Mohammed HS

更新日期：1988-03-01 00:00:00

abstract：:It was reported previously that HCV can be transmitted from persistently infected human bone-marrow-derived B-lymphoblastoid cells (TO.FE(HCV)) to human hepatoma cells by cell-to-cell contact. The present study confirms and characterize further such type of HCV infection in vitro. TO.FE(HCV) cells were co-cultured wit...

journal_title：Journal of medical virology

authors： Valli MB,Crema A,Lanzilli G,Serafino A,Bertolini L,Ravagnan G,Ponzetto A,Menzo S,Clementi M,Carloni G

更新日期：2007-10-01 00:00:00

abstract：:Previously, it was reported that productive viral infection, viral protein synthesis, and viral RNA replication of respiratory syncytial virus (RSV) operated efficiently in two human epithelial cell lines (HEp-2 and A549), but not in a human mast-cell line, HMC-1. Based on these observations, it was hypothesized that ...

journal_title：Journal of medical virology

authors： Shirato K,Ujike M,Kawase M,Matsuyama S

更新日期：2012-02-01 00:00:00

abstract：:We developed an immunoperoxidase staining test to detect structural antigens of BK virus (BKV) in Vero cell cultures. This test was used to examine the neutralizing activity of human and immunized animal sera. It was shown that sera positive for BKV antibodies measured by hemagglutination inhibition test and enzyme-li...

journal_title：Journal of medical virology

authors： Flaegstad T,Traavik T,Christie KE,Joergensen J

更新日期：1986-07-01 00:00:00

abstract：:A novel real-time PCR assay system was developed to quantify the cytomegalovirus (CMV) genome load. The real-time PCR assay could detect from 6 to over 10(6) copies of CMV-DNA with a wide linear range. The virus load of immunocompromised patients with symptomatic CMV infections was quantified and compared to that of a...

journal_title：Journal of medical virology

authors： Tanaka N,Kimura H,Iida K,Saito Y,Tsuge I,Yoshimi A,Matsuyama T,Morishima T

更新日期：2000-04-01 00:00:00

abstract：:A molecular epidemiological study on common diarrheal viruses was conducted in a children's hospital in Ho Chi Minh City between December 2005 and November 2006. Fecal samples were collected from 502 pediatric patients with acute gastroenteritis, and were screened for eight types of viral agents. Human astroviruses (H...

journal_title：Journal of medical virology

authors： Nguyen TA,Hoang L,Pham le D,Hoang KT,Mizuguchi M,Okitsu S,Ushijima H

更新日期：2008-02-01 00:00:00

abstract：:Until recently, human cytomegalovirus (hCMV) infection and anti-CMV treatment in transplanted patients have been monitored essentially by pp65 antigenemia, which is time-consuming and requires experienced operators. For the last two years, pp65 antigenemia levels have tended to be lower than previously in our laborato...

journal_title：Journal of medical virology

authors： Schvoerer E,Henriot S,Zachary P,Freitag R,Fuchs A,Fritsch S,Risch S,Meyer N,Caillard S,Lioure B,Stoll-Keller F

更新日期：2005-05-01 00:00:00

abstract：:Polyomavirus infection occurring during childhood is followed by a lifelong latency in immunocompetent subjects. The major site of polyomavirus persistence are the uroepithelial cells which leads to oral transmission. It has recently been hypothesized that tonsils could be a possible reservoir. The role of tonsil, ade...

journal_title：Journal of medical virology

authors： Comar M,Zanotta N,Bovenzi M,Campello C

更新日期：2010-07-01 00:00:00

abstract：:Hepatitis C Virus (HCV) genotype 4 predominates in Middle East and Central Africa countries. Recently, it has become also prevalent in Southern European countries where it is thought to have been introduced through immigration and the movement of intravenous drug users. In Italy, the prevalence of genotype 4 is partic...

journal_title：Journal of medical virology

authors： Ciccozzi M,Equestre M,Costantino A,Marascio N,Quirino A,Lo Presti A,Cella E,Bruni R,Liberto MC,Focà A,Pisani G,Zehender G,Ciccaglione AR

更新日期：2012-10-01 00:00:00

abstract：:It is well documented that perinatal transmission is the major cause of chronic HBV infection in China. However, the mechanisms of HBV perinatal transmission are not defined clearly. It is not known whether hepatitis B e antigen can cross the human placenta, and the rate of HBeAg decay in babies with and without HBV b...

journal_title：Journal of medical virology

authors： Wang Z,Zhang J,Yang H,Li X,Wen S,Guo Y,Sun J,Hou J

更新日期：2003-11-01 00:00:00

abstract：:The quantitation of serum levels of hepatitis C virus (HCV) RNA in chronic hepatitis C has been regarded as one of the most important indicators for the outcome of interferon (IFN) therapy. The AMPLICOR HCV MONITOR version 1.0 (AMPLICOR v1.0) assay is widely used for the evaluation of the HCV level. A new generation a...

journal_title：Journal of medical virology

authors： Kawai S,Yokosuka O,Imazeki F,Saisho H,Mizuno C

更新日期：2002-11-01 00:00:00

abstract：:The nature of the immunodominant a region of hepatitis B virus surface antigen (HBsAg) has been examined by mutation of specific amino acid residues. Proline 142 was required for the exhibition of full antigenicity. Replacement of cysteines 124 and 147 by serines drastically reduced or eliminated reactivity with antib...

journal_title：Journal of medical virology

authors： Ashton-Rickardt PG,Murray K

更新日期：1989-11-01 00:00:00

abstract：:Human bocavirus (HBoV) was first recognized in respiratory samples in 2005. The clinical importance of HBoV infection remains unclear. This report describes the clinical features and molecular phylogeny of HBoV isolates in children with acute respiratory infections. Nasopharyngeal aspirates were obtained from 1,528 ch...

journal_title：Journal of medical virology

authors： Ahn JG,Choi SY,Kim DS,Kim KH

更新日期：2014-12-01 00:00:00

abstract：:This study analyzed the genotype distribution and frequency of lamivudine (LAM) and tenofovir (TDF) resistance mutations in a group of patients co-infected with HIV and hepatitis B virus (HBV). A cross-sectional study of 847 patients with HIV was conducted. Patients provided blood samples for HBsAg detection. The load...

journal_title：Journal of medical virology

authors： Mendes-Correa MC,Pinho JR,Locarnini S,Yuen L,Sitnik R,Santana RA,Gomes-Gouvêa MS,Leite OM,Martins LG,Silva MH,Gianini RJ,Uip DE

更新日期：2010-09-01 00:00:00

abstract：:Most dengue virus (DENV) infections remain asymptomatic. This increases the risk of DENV transfusion transmission (TT-DENV) during outbreaks. We evaluated DENV viremia in 8475 blood donations assembled in minipools for the presence of DENV RNA. The tested samples were obtained between February and May, 2016, during a ...

journal_title：Journal of medical virology

authors： Slavov SN,Santos EV,Hespanhol MR,Rodrigues ES,Haddad R,Ubiali EMA,Covas DT,Kashima S

更新日期：2020-10-22 00:00:00

abstract：PROBLEM:Objective studies that use validated questionnaires are needed to evaluate the changes in the sexual functions of women diagnosed with human papillomavirus (HPV) infection. METHOD OF STUDY:The study comprised 80 sexually active women diagnosed with a high-risk HPV infection. These patients were divided into fo...

journal_title：Journal of medical virology

authors： Alay I,Kaya C,Karaca I,Yildiz S,Baghaki S,Cengiz H,Ekin M,Yasar L

更新日期：2020-08-01 00:00:00

abstract：:A common causative agent for uterine cervical cancer is the human papillomavirus type 18 (HPV-18) which has three phylogenic variants: Asian-Amerindian, European, and African. Each variant shows significant molecular differences in the E6 gene. E6 oncoprotein is a negative regulator of tumor suppressor protein p53, he...

journal_title：Journal of medical virology

authors： Vazquez-Vega S,Sanchez-Suarez LP,Andrade-Cruz R,Castellanos-Juarez E,Contreras-Paredes A,Lizano-Soberon M,Garcia-Carranca A,Benitez Bribiesca L

更新日期：2013-07-01 00:00:00

abstract：:This is a case study of a child who developed roseola infantum first, then varicella, and was later affected by acute cerebellar syndrome, severe truncal ataxia, and myoclonic dystonia. Human herpesvirus 6 (HHV-6) A and B were detected in the cerebrospinal fluid (CSF) and peripheral blood, respectively, upon ataxia on...

journal_title：Journal of medical virology

authors： Borghi E,Pagani E,Mancuso R,Delbue S,Valli M,Mazziotti R,Giordano L,Micheli R,Ferrante P

更新日期：2005-03-01 00:00:00

abstract：:An outbreak of an acute sporadic viral hepatitis occurring at three villages in the lower Shebli region of southern Somalia was studied. Sera were examined for antibodies to hepatitis E virus (HEV) antigen by a recently developed enzyme-linked immunosorbent assay. The prevalence of anti-HEV ranged from 77.8% to 94.0% ...

journal_title：Journal of medical virology

authors： Mushahwar IK,Dawson GJ,Bile KM,Magnius LO

更新日期：1993-07-01 00:00:00

abstract：:Here, we studied the role of nitric oxide (NO) production during the first steps of the respiratory infection of BALB/c mice with herpes simplex virus type 1 (HSV-1), strain F. Nitric oxide synthase II (NOS-II) mRNA and protein were detected by reverse transcription (RT)-PCR and dot blot, respectively in samples of lu...

journal_title：Journal of medical virology

authors： Gamba G,Cavalieri H,Courreges MC,Massouh EJ,Benencia F

更新日期：2004-06-01 00:00:00

abstract：:Anal cancer is a rare disease. Nevertheless, it may be a reason for concern among groups in which its incidence is increasing: those who engage in anoreceptive intercourse, promiscuous persons, and those with sexually transmitted infections (HPV and HIV). The aim of this study was to evaluate the prevalence of abnorma...

journal_title：Journal of medical virology

authors： Chaves EB,Folgierini H,Capp E,von Eye Corleta H

更新日期：2012-09-01 00:00:00

abstract：:The natural history of chronic hepatitis B virus (HBV) infection is highly variable, ranging from a benign course to one of shortened life expectancy. Liver histology represents an accurate tool for assessing progressive liver disease, and has been used in five recent Phase III clinical trials of the oral nucleoside a...

journal_title：Journal of medical virology

authors： Leung N

更新日期：2000-07-01 00:00:00

abstract：:Enterovirus infections are usually mild but can also cause severe illnesses and play a role in chronic diseases, such as cardiomyopathies and type 1 diabetes. Host response to the invading virus can markedly modulate the course of the infection, and this response varies between individuals due to the polymorphism of i...

journal_title：Journal of medical virology

authors： Hämäläinen S,Nurminen N,Ahlfors H,Oikarinen S,Sioofy-Khojine AB,Frisk G,Oberste MS,Lahesmaa R,Pesu M,Hyöty H

更新日期：2014-08-01 00:00:00

abstract：:Latency associated peptide (LAP) is a protein expressed on the membrane of some regulatory T cells (Treg). LAP+ Treg have a greater immunomodulatory effect than that of their negative counterparts. In this study, we presented the data on the proportion of LAP+ Treg out of CD4+ cells in mice with viral myocarditis, whi...

journal_title：Journal of medical virology

authors： Wang X,Ge J,Chen R

更新日期：2019-05-01 00:00:00

abstract：:The presence of hepatitis GB virus C (GBV-C), also known as hepatitis G virus (HGV), and hepatitis C virus (HCV) were investigated in sera from 45 hemophiliacs from nine locations in Nicaragua using a nested polymerase chain reaction (PCR). Primers used to detect GBV-C and HCV derived from the helicase region and 5'UT...

journal_title：Journal of medical virology

authors： González-Pérez MA,Norder H,Bergström A,Lopéz E,Visoná KA,Magnius LO

更新日期：1997-06-01 00:00:00

abstract：:The relative frequency and distribution of the VP4 (P) genotypes of 227 human rotavirus field strains were investigated in South Africa. The stool samples were collected between 1984-1993 from infants and young children with diarrhea at Ga-Rankuwa Hospital, Pretoria, South Africa. The RNA was extracted from stools, he...

journal_title：Journal of medical virology

authors： Mphahlele MJ,Steele AD

更新日期：1995-09-01 00:00:00

abstract：:Stool specimens from 334 infants and young children hospitalized with diarrhea in the General Hospital, Kuala Lumpur, Malaysia between August and November, 1987 were analyzed for the presence of rotavirus double-stranded (ds) RNA by polyacrylamide gel electrophoresis. Of the 334 specimens analyzed, 32 (9.6%) were posi...

journal_title：Journal of medical virology

authors： Rasool NB,Hamzah M,Jegathesan M,Wong YH,Qian Y,Green KY

更新日期：1994-07-01 00:00:00

abstract：:Detection of respiratory viruses using polymerase chain reaction (PCR) is sensitive, specific and cost effective, having huge potential for patient management. In this study, the performance of an in-house developed conventional multiplex RT-PCR (mRT-PCR), real time RT-PCR (rtRT-PCR) and Luminex xTAG(®) RVP fast assay...

journal_title：Journal of medical virology

authors： Choudhary ML,Anand SP,Tikhe SA,Walimbe AM,Potdar VA,Chadha MS,Mishra AC

更新日期：2016-01-01 00:00:00

abstract：:A solution of pooled cryo-precipitate for preparing factor VIII (F VIII) by the solvent/detergent method was contaminated experimentally with parvovirus B19-positive plasma to evaluate virus reduction achieved by the final steps of the F VIII production process. Virus reduction was at least 2 logs of the total amount ...

journal_title：Journal of medical virology

authors： Schwarz TF,Roggendorf M,Hottenträger B,Stolz W,Schwinn H

更新日期：1991-09-01 00:00:00