Abstract:
:A solution of pooled cryo-precipitate for preparing factor VIII (F VIII) by the solvent/detergent method was contaminated experimentally with parvovirus B19-positive plasma to evaluate virus reduction achieved by the final steps of the F VIII production process. Virus reduction was at least 2 logs of the total amount of B19 virus added to the pooled cryo-precipitate. The major amount of B19 virus was detected in the solution used to regenerate the F VIII-selective anion exchange chromatography column. A few viral particles were detected in the final F VIII concentrate before filtration but these were aggregates and were removed by filtration, and in the solution used to regenerate the anion exchange resin. It is not known whether the residual viral DNA present in the final product represents infectious or inactivated particles.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Schwarz TF,Roggendorf M,Hottenträger B,Stolz W,Schwinn Hdoi
10.1002/jmv.1890350107subject
Has Abstractpub_date
1991-09-01 00:00:00pages
28-31issue
1eissn
0146-6615issn
1096-9071journal_volume
35pub_type
杂志文章abstract::Recently developed monoclonal antibodies against the 42,000-dalton major inner capsid protein were used in an enzyme immunoassay to subgroup a total of 156 rotavirus specimens obtained from Japanese infants and young children with acute gastroenteritis during the period between December 1981 and April 1983. Only 2 spe...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890170105
更新日期:1985-09-01 00:00:00
abstract::Dengue virus (DV) infection can result in either a mild febrile illness known as dengue fever (DF) or a life-threatening disease called dengue hemorrhagic fever (DHF). DHF is more prevalent in patients undergoing secondary DV infection. This observation has led to the hypothesis that DHF may be the result of immune re...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.2037
更新日期:2001-10-01 00:00:00
abstract::We studied the role of sexual transmission in the epidemiology of HCV by a cross-sectional study comparing prostitutes and HCV seropositive and seronegative sexual clients recruited from the bordellos of the docks of Santos, São Paulo State. The average age in the prostitute group was 27.2 years. The median time spent...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:1997-04-01 00:00:00
abstract::Cervical specimens collected from 163 cytologically healthy women were screened for the presence of human papillomavirus (HPV) DNA and anti-HPV secretory IgA antibodies. HPV DNA was detected by a general primer mediated polymerase chain reaction (PCR), which amplifies a conserved region from the L1 ORF of genital HPVs...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890430219
更新日期:1994-06-01 00:00:00
abstract::B-cells play an important role in defending children against various infections. In view of scare data, we undertook this prospective cohort study to describe B cell compartment in HIV infected children (<5 years of age) and the effect of HAART on B cell subpopulations. HIV infected children (<5 years) from Pediatric ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25074
更新日期:2018-07-01 00:00:00
abstract::This study aimed to examine the application of hepatitis B surface antigen (HBsAg) confirmatory testing when diagnosing hepatitis B infection among young persons in Taiwan with a low prevalence rate of hepatitis B infection. HBsAg status, the presence of antibodies against HBsAg (anti-HBs), and the presence of antibod...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.22127
更新日期:2011-09-01 00:00:00
abstract::Two hundred and thirty fecal specimens were collected from children (up to 5 years of age) admitted with suspected rotaviral gastroenteritis at four Irish hospitals (Cork University Hospital, Mercy Hospital, Cork, Waterford Regional Hospital, and Kerry General Hospital) in the southern region of Ireland, between 2001 ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20494
更新日期:2005-12-01 00:00:00
abstract::Dual infection with hepatitis B and C viruses is often encountered in endemic areas of both viruses. However, understanding of the clinical and virological implications is limited. The aim of this study was to investigate the role of each virus in liver injury and the interaction between the two viruses in dual infect...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890460316
更新日期:1995-07-01 00:00:00
abstract::The 30-base pair (bp) deletion of the cytoplasmic carboxy-terminal domain of the latent membrane protein 1 (LMP-1) gene was analyzed in 37 frozen tissues from patients with Epstein-Barr virus (EBV)-related gastric cancer and 18 throat washings from healthy adults in southern Japan. The 30-bp deletion was identified in...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(199902)57:2<152::aid-jmv11
更新日期:1999-02-01 00:00:00
abstract::Infection with putative non-A to E hepatitis virus, designated GB virus C (GBV-C), was surveyed in 286 patients with chronic liver disease in Japan. RNA of GBV-C was detected, by reverse-transcription polymerase chain reaction with nested primers from the 5'-noncoding region, in 19 patients (6.6%) at a frequency highe...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:1997-03-01 00:00:00
abstract::Hepatitis E virus (HEV) infection has become a global concern with high mortality rates among pregnant women, especially those in their third trimester of pregnancy. Estrogen plays an important role in mediating the body, regulating physiological and pathological processes. Estrogen is activated by binding to estrogen...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26641
更新日期:2020-10-31 00:00:00
abstract::Ultra violet (UV) light-inactivated measles virus was used for the lymphocyte proliferation assay, where it caused consistently adult human peripheral blood monolymphocytes (PBML) proliferation which was not dependent on the measles virus complement fixation titre in the donor's sera. When the cord blood lymphocytes f...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890460209
更新日期:1995-06-01 00:00:00
abstract::Because of the distinct ability of retroviruses to integrate into the target cell genome and thus achieve long-term expression, retrovirus vectors hold great promise for stable gene transfer. Such vectors derived from human immunodeficiency retroviruses (HIVs) and other lentiviruses are envisioned to possess several a...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(199802)54:2<118::aid-jmv9>
更新日期:1998-02-01 00:00:00
abstract::BK virus (BKV) establishes a persistent, asymptomatic infection in more than 80% of the human population, and in immunocompromised individuals BKV causes acute urinary tract infections. Two forms of BKV, archetype and rearranged, have been recovered previously from urine samples. The rearranged form is believed to hav...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:2000-03-01 00:00:00
abstract::Studies of hepatitis B virus (HBV) infection in non-human primates such as chimpanzees are no longer possible due to ethical considerations and the endangered status of chimpanzees since April 2007 in Japan. A human hepatocyte transplanted chimeric mouse was used to characterize HBV infectivity in serial stages of acu...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21320
更新日期:2008-12-01 00:00:00
abstract::Human parechoviruses (HPeV) are associated with central nervous system (CNS) infections and sepsis-like illnesses. However, data from China are not available. The aim of this study was to determine the prevalence, age, and seasonal distributions and genotypes of HPeV infections in children with CNS related disease in ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23449
更新日期:2013-02-01 00:00:00
abstract::Limited data are available describing human papillomavirus (HPV) genotype distribution among females with cytological abnormalities in Switzerland. Cervical cell specimens obtained from 5,318 women were screened routinely by liquid-based Pap smear. All specimens with cellular abnormalities were analyzed subsequently f...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.22126
更新日期:2011-08-01 00:00:00
abstract::Human metapneumovirus, with two known genotypes named A and B, is associated with mild respiratory symptoms to severe LRTI in children, high-risk adults and the elderly. Rapid and reliable methods of hMPV detection in clinical samples are essential to implement appropriate care, to better understand the pathology of h...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21038
更新日期:2008-01-01 00:00:00
abstract::The molecular epidemiology of hepatitis A virus (HAV) strains circulating in the St. Petersburg and Karelia regions was studied during 1997-2003. Hepatitis A virus RNA was isolated from both clinical samples (stools or sera) and environmental samples (sewage water). RT-PCR was carried out using different primer pairs ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20843
更新日期:2007-06-01 00:00:00
abstract::Herpes simplex virus can be quantitatively recovered from vaginal tampons suspended in tissue culture medium and stored over a wide range of temperatures. Because herpes simplex virus is stable for several days with refrigeration or after freezing, this method of culture of cervicovaginal virus lends itself to epidemi...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890030306
更新日期:1979-01-01 00:00:00
abstract::To evaluate the variation over time of seroreactivity to human papillomavirus type 11 (HPV-11) according to disease outcome, we selected a sample of 42 condyloma acuminatum patients from a group of subjects enrolled in a placebo-controlled trial of three alpha-interferon preparations administered parenterally for the ...
journal_title:Journal of medical virology
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1002/jmv.1890390414
更新日期:1993-04-01 00:00:00
abstract::Recruitment of leukocytes by chemokines and chemokine receptors to CNS plays a crucial role in the induction of inflammatory response in HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). In the present study, chemokine and chemokine receptors involved in trafficking of lymphocytes to the CNS were me...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24779
更新日期:2017-08-01 00:00:00
abstract::This study analyzed the genotype distribution and frequency of lamivudine (LAM) and tenofovir (TDF) resistance mutations in a group of patients co-infected with HIV and hepatitis B virus (HBV). A cross-sectional study of 847 patients with HIV was conducted. Patients provided blood samples for HBsAg detection. The load...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21845
更新日期:2010-09-01 00:00:00
abstract::An "in-house" recombinant virus protease inhibitor susceptibility assay was carried out (median of 3 per patient) retrospectively in 26 patients failing HIV protease inhibitor based therapy at regular intervals to the initiation of the first protease inhibitor. Patients were treated with either indinavir (N = 6), rito...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10076
更新日期:2002-07-01 00:00:00
abstract::Liver fibrosis is accelerated in human immunodeficiency virus/hepatitis C virus (HIV/HCV) coinfected compared with HCV monoinfected patients, due to multiple cofactors. Recently, HLA-B18 haplotype has been associated with short-term liver disease progression in this population. Our aim was to assess the influence of H...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25385
更新日期:2019-05-01 00:00:00
abstract::Genital herpes is a common, distressing infection whose incidence has been underestimated. Recent serological surveys, employing type-specific antibody assays, show a rising prevalence of previous herpes simplex virus (HSV) type 2 infections in post-adolescent populations in developed countries; many of these infectio...
journal_title:Journal of medical virology
pub_type: 杂志文章,评审
doi:10.1002/jmv.1890410508
更新日期:1993-01-01 00:00:00
abstract::The purpose of this study was to evaluate the use of dried blood spots stored on filter paper as a means to provide specimens for virologic surveillance for measles virus (MV) in situations when the reverse cold chain is not available. Two single-step RT-PCR assays were evaluated for sensitivity of detection of MV nuc...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10144
更新日期:2002-08-01 00:00:00
abstract::Mixed cryoglobulinemia is a lymphoproliferative disorder associated with hepatitis C virus (HCV). In patients chronically affected by HCV the prevalence of mixed cryoglobulinemia is variable ranging from 0% to 56%. To verify whether polyomaviruses (PyV) play a role in this disorder a total of 222 blood samples from 63...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23867
更新日期:2014-04-01 00:00:00
abstract::There are contradictory reports regarding the effects of inhalation anesthetics on the immune system. Measurable immune responses have been studied in vitro, but little is known about the in vivo effects in the intact organism. We used an attenuated, non-neuroinvasive, nonlethal strain of the encephalitic West Nile vi...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.2184
更新日期:2002-04-01 00:00:00
abstract::Human herpesvirus-8 (HHV-8) causes Kaposi's sarcoma (KS) and lymphoproliferative disorders in both HIV-infected and uninfected patients. HHV-8 has a worldwide occurrence but infection rates vary according to a combination of geographic and behavioral risks. The main transmission route seems to be sexual, nevertheless,...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20029
更新日期:2004-04-01 00:00:00