Neutralization test for BK virus: plaque reduction detected by immunoperoxidase staining.

Abstract:

:We developed an immunoperoxidase staining test to detect structural antigens of BK virus (BKV) in Vero cell cultures. This test was used to examine the neutralizing activity of human and immunized animal sera. It was shown that sera positive for BKV antibodies measured by hemagglutination inhibition test and enzyme-linked immunosorbent assay (ELISA) were able to prevent expression of BKV structural antigens in cell cultures. The correlation between titers in the hemagglutination inhibition test, levels of BKV IgG measured by ELISA, and the titers assayed by the immunoperoxidase neutralization test was high. We suggest that this type of test may be used instead of conventional neutralization tests for other viruses with slowly developing cytopathogenic effects.

journal_name

J Med Virol

authors

Flaegstad T,Traavik T,Christie KE,Joergensen J

doi

10.1002/jmv.1890190311

subject

Has Abstract

pub_date

1986-07-01 00:00:00

pages

287-96

issue

3

eissn

0146-6615

issn

1096-9071

journal_volume

19

pub_type

杂志文章
  • HCV RNA in patients with chronic hepatitis C treated with interferon-alpha.

    abstract::In order to assess the efficacy of interferon-alpha on hepatitis C viral RNA in patients with chronic hepatitis C, we analyzed the levels of HCV RNA in sera and liver tissues of 16 patients pre- and posttreatment using a semiquantitative polymerase chain reaction method. Fifteen of 16 patients were positive for anti-H...

    journal_title:Journal of medical virology

    pub_type: 临床试验,杂志文章,多中心研究

    doi:10.1002/jmv.1890400114

    authors: Liang TJ,Rustgi V,Galun E,Blum HE

    更新日期:1993-05-01 00:00:00

  • High frequency of lamivudine resistance mutations in Brazilian patients co-infected with HIV and hepatitis B.

    abstract::This study analyzed the genotype distribution and frequency of lamivudine (LAM) and tenofovir (TDF) resistance mutations in a group of patients co-infected with HIV and hepatitis B virus (HBV). A cross-sectional study of 847 patients with HIV was conducted. Patients provided blood samples for HBsAg detection. The load...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21845

    authors: Mendes-Correa MC,Pinho JR,Locarnini S,Yuen L,Sitnik R,Santana RA,Gomes-Gouvêa MS,Leite OM,Martins LG,Silva MH,Gianini RJ,Uip DE

    更新日期:2010-09-01 00:00:00

  • The impact of high risk human papillomavirus testing in an inner London colposcopy clinic.

    abstract::This is an audit of a new technique to improve the colposcopy service. Samples were tested for high risk HPV DNA using Digene Hybrid Capture II. Sixty-four percent of the sampled women under 30 had detectable high risk HPV DNA, decreasing to 44% in 30--39 year olds and to 27% in women over 40. High risk HPV prevalence...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20401

    authors: Perrons C,Brink N,Jalal H,Watts P,Jelley R

    更新日期:2005-08-01 00:00:00

  • Comprehensive investigation of the presence of JC virus in AIDS patients with and without progressive multifocal leukoencephalopathy.

    abstract::Progressive multifocal leukoencephalopathy (PML), a viral-induced demyelinating disease, is becoming relatively common, while many diagnostic and pathogenetic aspects remain to be clarified. A study was therefore undertaken in 64 AIDS patients suffering from various neurological disorders, including PML (12 subjects),...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/(sici)1096-9071(199707)52:3<235::aid-jmv1>

    authors: Ferrante P,Caldarelli-Stefano R,Omodeo-Zorini E,Cagni AE,Cocchi L,Suter F,Maserati R

    更新日期:1997-07-01 00:00:00

  • Comparative evaluation of measles virus-specific RT-PCR methods through an international collaborative study.

    abstract::Comparison of RT-PCR assays established in house at various places revealed that laboratories could differ in sensitivity by as much as 1,000-fold in terms of the ability to detect measles virus sequences in clinical samples. The study indicates that PCR findings, positive or negative, are questionable if they are not...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.10371

    authors: Afzal MA,Osterhaus AD,Cosby SL,Jin L,Beeler J,Takeuchi K,Kawashima H

    更新日期:2003-05-01 00:00:00

  • Effect of Phyllanthus amarus on duck hepatitis B virus replication in vivo.

    abstract::Nine ducks congenitally infected with the duck hepatitis B virus (DHBV) were treated either orally (four ducks for 10 weeks) or intraperitoneally (five ducks for 12 weeks) with the Indian traditional herbal remedy Phyllanthus amarus. Compared to placebo-treated control ducks, these treatments did not result in a reduc...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890320404

    authors: Niu JZ,Wang YY,Qiao M,Gowans E,Edwards P,Thyagarajan SP,Gust I,Locarnini S

    更新日期:1990-12-01 00:00:00

  • Significant impact of non-B HIV-1 variants genetic diversity in Gabon on plasma HIV-1 RNA quantitation.

    abstract::Evaluations of HIV-1 RNA viral load assays are lacking in Central Africa. The main objective of our study was to assess the reliability of HIV-1 RNA results obtained with three different assays for samples collected in Gabon. A total of 137 plasma specimens were assessed for HIV-1 RNA using the Abbott RealTime HIV-1® ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.23770

    authors: Mouinga-Ondémé A,Mabika-Mabika A,Alalade P,Mongo AD,Sica J,Liégeois F,Rouet F

    更新日期:2014-01-01 00:00:00

  • Characterization of in vitro dengue virus resistance to carrageenan.

    abstract::The λ-carrageenan (λ-car) is a potent and selective inhibitor of dengue virus (DENV) infection targeted to virus adsorption and internalization, due to the structural similarities with the mammalian cell receptor heparan sulfate. To further characterize the antiviral activity of λ-car, the selection and the phenotypic...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.24457

    authors: Talarico LB,Damonte EB

    更新日期:2016-07-01 00:00:00

  • Infectivity titration of a prototype strain of hepatitis E virus in cynomolgus monkeys.

    abstract::The infectivity titer of a standard stock of the SAR-55 strain of hepatitis E virus (HEV) was determined in cynomolgus macaques (Macaca fascicularis) and the effect of dose on the course of the infection was examined by weekly monitoring of alanine aminotransferase (ALT) and anti-HEV levels. Antibody to HEV (anti-HEV)...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890430207

    authors: Tsarev SA,Tsareva TS,Emerson SU,Yarbough PO,Legters LJ,Moskal T,Purcell RH

    更新日期:1994-06-01 00:00:00

  • Characterization of immune complexes containing cytomegalovirus-specific IgM antibodies following a kidney graft.

    abstract::In order to demonstrate the viral specificity of IgM-containing immune complexes (IgM-CIC) detected by a C1q assay in renal allograft recipients developing a CMV infection, a technique is described allowing: 1) the dissociation of IgM-CIC by action of an acid buffer, and 2) the characterization of the viral specificit...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890330312

    authors: Basson J,Tardy JC,Aymard M

    更新日期:1991-03-01 00:00:00

  • Real-time PCR for detection and quantitation of hepatitis B virus DNA.

    abstract::A sensitive and reproducible real-time PCR assay based on TaqMan technology was developed for the detection and quantitation of hepatitis B virus (HBV) DNA in serum, and compared with an "in-house" qualitative PCR assay. HBV DNA was measured in 125 serum samples from 76 hepatitis B patients, consisting of 22 patients ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.2027

    authors: Chen RW,Piiparinen H,Seppänen M,Koskela P,Sarna S,Lappalainen M

    更新日期:2001-10-01 00:00:00

  • Molecular characterization of group A rotavirus found in elderly patients in Ireland; predominance of G1P[8], continued presence of G9P[8], and emergence of G2P[4].

    abstract::Rotavirus is a major cause of gastroenteritis in young children worldwide. There have been several recent reports concerning rotavirus isolation from adults, particularly in the elderly, presenting with gastroenteritis. In this study, the authors report on rotavirus outbreaks in five separate elderly care facilities b...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.23416

    authors: Gunn L,Feeney SA,Cashman O,Collins PJ,Coyle PV,O'Shea H

    更新日期:2012-12-01 00:00:00

  • Heterogeneous nature of HIV-1 recombinants spreading in Spain.

    abstract::HIV-1 infections due to non-B subtypes are increasing rapidly in number and spreading across Europe. The genetic nature of HIV-1 non-B variants containing subtype G sequences at the protease (PR)-coding region are described from 48 unrelated subjects living in Spain. Phylogenetic analyses of the HIV-1 reverse transcri...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20280

    authors: Holguín A,Alvarez A,Soriano V

    更新日期:2005-03-01 00:00:00

  • Hepatitis E viral infection regulates estrogen signaling pathways: Inhibition of the cAMPK-PKA-CREB and PI3K-AKT-mTOR signaling pathways.

    abstract::Hepatitis E virus (HEV) infection has become a global concern with high mortality rates among pregnant women, especially those in their third trimester of pregnancy. Estrogen plays an important role in mediating the body, regulating physiological and pathological processes. Estrogen is activated by binding to estrogen...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.26641

    authors: Gong S,Hao X,Bi Y,Yang C,Wang W,Mickael HK,Zhang Y,Chen S,Qian Z,Huang F,Wei D,Yu W

    更新日期:2020-10-31 00:00:00

  • Epidemiology of children with acquired immune deficiency syndrome (stage 3): A referral hospital-based study in Iran.

    abstract::Lack of recognition of human immunodeficiency virus (HIV) infection especially in children and delayed implementation of effective control programs makes HIV infection as a major cause for concern. Information on HIV epidemiology in Iran as well as other Islamic countries is limited. The aim of our study was to descri...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.24294

    authors: Movahedi Z,Mahmoudi S,Pourakbari B,Keshavarz Valian N,Sabouni F,Ramezani A,Bahador A,Mamishi S

    更新日期:2016-01-01 00:00:00

  • Inhibition of hepatitis C virus NS3 function by antisense oligodeoxynucleotides and protease inhibitor.

    abstract::Hepatitis C Virus (HCV) NS3 protease is an attractive target for antiviral agent development because it is required for viral replication. Because a stable cell culture system or small animal model to study HCV replication is not readily available, we constructed an in vitro model allowing the investigation of NS3 tra...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.2089

    authors: Heintges T,Encke J,zu Putlitz J,Wands JR

    更新日期:2001-12-01 00:00:00

  • Evaluation of LIAISON® XL system for HBsAg, and anti-HCV and anti-HIV/Ag p24.

    abstract::The aim of this study was to compare the data obtained using the new LIAISON® XL chemiluminescence system to search for HBsAg, anti-HCV, and anti-HIV1-2/p24 Ag with those obtained using the VITROS system currently adopted by the Microbiology Unit of the Hospital of Legnano. Routine samples of patients who were referre...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.24648

    authors: De Paschale M,Manco MT,Belvisi L,Cagnin D,Cerulli T,Paganini A,Arpino O,Cianflone A,Agrappi C,Mirri P,Clerici P

    更新日期:2017-03-01 00:00:00

  • Scoring system for risk stratification of viral reactivation during prophylactic antiviral treatment in Korean patients with hepatitis B undergoing anticancer chemotherapy: A multicenter study.

    abstract::Prophylactic antiviral therapy is recommended for hepatitis B virus (HBV)-infected patients with malignancies who are undergoing systemic chemotherapy. In the current study, we aimed to develop a risk scoring system to guide the selection of prophylactic antiviral agents. In this retrospective analysis, we included co...

    journal_title:Journal of medical virology

    pub_type: 杂志文章,多中心研究

    doi:10.1002/jmv.25241

    authors: Kim HY,Yoo JJ,Oh S,Yu SJ,Kim YJ,Yoon JH,Kim W,Jung YJ,Kim BH,Kim CM,Park JW,Lee JH

    更新日期:2018-10-01 00:00:00

  • Hepatitis B virus genotype D strains from Estonia share sequence similarity with strains from Siberia and may specify ayw4.

    abstract::The genotypes and subtypes of 205 HBV isolates collected during 1989-2002 in Estonia and 14 other regions of the former USSR were determined by sequencing and phylogenetic analysis of the S gene. The in Europe prevailing genotypes, A and D, were also circulating in the whole territory of the former USSR including Esto...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20169

    authors: Tallo T,Norder H,Tefanova V,Krispin T,Priimägi L,Mukomolov S,Mikhailov M,Magnius LO

    更新日期:2004-10-01 00:00:00

  • Genetic relatedness of Cuban HAV wild-type isolates.

    abstract::Knowledge of the nucleotide sequence in the region of the putative VP1/2A junction of the Hepatitis A virus (HAV) genome has enabled differentiation of HAV strains and their classification into seven genotypes, in some of which sub-genotypes A and B can be defined. A 168 base segment encompassing the putative VP1/2A j...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1023

    authors: Diaz BI,Sariol CA,Normann A,Rodríguez L,Flehmig B

    更新日期:2001-06-01 00:00:00

  • Serological studies of an enterically transmitted non-A, non-B hepatitis in Somalia.

    abstract::An outbreak of an acute sporadic viral hepatitis occurring at three villages in the lower Shebli region of southern Somalia was studied. Sera were examined for antibodies to hepatitis E virus (HEV) antigen by a recently developed enzyme-linked immunosorbent assay. The prevalence of anti-HEV ranged from 77.8% to 94.0% ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890400309

    authors: Mushahwar IK,Dawson GJ,Bile KM,Magnius LO

    更新日期:1993-07-01 00:00:00

  • Titration of hepatitis B virus infectivity in the sera of pre-acute and late acute phases of HBV infection: transmission experiments to chimeric mice with human liver repopulated hepatocytes.

    abstract::Studies of hepatitis B virus (HBV) infection in non-human primates such as chimpanzees are no longer possible due to ethical considerations and the endangered status of chimpanzees since April 2007 in Japan. A human hepatocyte transplanted chimeric mouse was used to characterize HBV infectivity in serial stages of acu...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21320

    authors: Tabuchi A,Tanaka J,Katayama K,Mizui M,Matsukura H,Yugi H,Shimada T,Miyakawa Y,Yoshizawa H

    更新日期:2008-12-01 00:00:00

  • Significance of detectable HCV RNA below the limit of quantification in patients treated with DAAs using standard and ultrasensitive protocols.

    abstract::Predictive factors of HCV relapse after treatment with DAAs are poorly understood. In this study, we aimed to assess whether the residual viral load positivity observed during or at the end of treatment (EOT) has an impact on viral outcome. Blood samples were collected from 337 patients with genotypes (GT) 1a, 1b, 2, ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.25084

    authors: Visco-Comandini U,Lapa D,Lionetti R,Taibi C,Loiacono L,Montalbano M,Capobianchi MR,D'Offizi G,Garbuglia AR

    更新日期:2018-07-01 00:00:00

  • Genetic variability of German hepatitis C virus isolates.

    abstract::Heterogeneity of hepatitis C viral (HCV) genomes of several isolates from different countries has been reported, but there is little information on HCV isolates for the Federal Republic of Germany. Therefore, the nucleotide (nt) and deduced amino acid (aa) sequences of interesting parts of the viral genome derived fro...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890400407

    authors: Müller HM,Pfaff E,Goeser T,Theilmann L

    更新日期:1993-08-01 00:00:00

  • Spontaneously-resolving episodes of cytomegalovirus DNAemia in allogeneic hematopoietic stem cell transplant recipients: Virological features and clinical outcomes.

    abstract::It has been reported that low-plasma cytomegalovirus (CMV) DNA loads are associated with an increased risk of overall mortality in allogeneic hematopoietic stem cell transplantation (allo-HSCT). Utilizing a conservative strategy for initiation of preemptive antiviral therapy (>1500 IU/mL), we characterized the virolog...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.25426

    authors: Talaya A,Giménez E,Piñana JL,Albert E,Hernández-Boluda JC,Pérez A,Torres I,Solano C,Navarro D

    更新日期:2019-06-01 00:00:00

  • Viruses in community-acquired pneumonia in children aged less than 3 years old: High rate of viral coinfection.

    abstract::The occurrence of viral coinfections in childhood pneumonia has received little attention, probably because suitable detection methods have been lacking. Between November 2004 and October 2006, the presence of 14 respiratory viruses in children aged less than 3 years old with community-acquired pneumonia were investig...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21271

    authors: Cilla G,Oñate E,Perez-Yarza EG,Montes M,Vicente D,Perez-Trallero E

    更新日期:2008-10-01 00:00:00

  • Serologic and viral genome prevalence of HSV, EBV, and HCMV among healthy adults in Wuhan, China.

    abstract::The worldwide infection rate of herpesvirus is high, but the detailed prevalence in China, especially the central area, remains unclear. In the present study, the prevalence of herpes simplex virus (HSV), Epstein-Barr virus (EBV), and human cytomegalovirus (HCMV) was investigated in 303 healthy adults in Wuhan, a repr...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.24989

    authors: Wen L,Qiu Y,Cheng S,Jiang X,Ma YP,Fang W,Wang W,Cui J,Ruan Q,Zhao F,Hu F,Luo MH

    更新日期:2018-03-01 00:00:00

  • Seasonality and survival associated with three outbreak seasons of West Nile virus disease in Oklahoma--2003, 2007, and 2012.

    abstract::West Nile virus (WNV) activity has fluctuated in the south-central United States since its introduction. Seasonal outbreaks are common, with three in Oklahoma during 2003, 2007, and 2012. Morbidity and mortality rates vary during each outbreak. Long-term neurologic sequelae in association with West Nile virus disease ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.24235

    authors: Johnson MG,Adams J,McDonald-Hamm C,Wendelboe A,Bradley KK

    更新日期:2015-10-01 00:00:00

  • Evidence of shared Epstein-Barr viral isolates between sexual partners, and low level EBV in genital secretions.

    abstract::Epstein-Barr virus is present in the saliva of most persistently infected individuals and is generally thought to be spread by close oral contact. However, there are now several reports of EBV in genital secretions, suggesting the possibility of sexual transmission between adults. The present study was undertaken to i...

    journal_title:Journal of medical virology

    pub_type: 杂志文章,多中心研究

    doi:10.1002/jmv.20682

    authors: Thomas R,Macsween KF,McAulay K,Clutterbuck D,Anderson R,Reid S,Higgins CD,Swerdlow AJ,Harrison N,Williams H,Crawford DH

    更新日期:2006-09-01 00:00:00

  • Analysis of influenza A H3N2 strains isolated in England during 1995-1996 using polymerase chain reaction restriction.

    abstract::A polymerase chain reaction-restriction (PCR-restriction) endonuclease assay was developed to allow rapid analysis of influenza A H3N2 viruses circulating in England during 1995-1996. Restriction endonuclease digestion with two enzymes of amplicons derived from PCR of the HA1 portion of the influenza haemagglutinin (H...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:

    authors: Ellis JS,Sadler CJ,Laidler P,de Andrade HR,Zambon MC

    更新日期:1997-03-01 00:00:00