Abstract:
:We have attempted to reactivate human herpesvirus 6 (HHV-6) by infection with HHV-7 using childhood exanthem subitum patients in vitro. Peripheral blood mononuclear cells (PBMCs) were collected from children who had a history of exanthem subitum(ES) by HHV-6 and were infected by human herpesvirus 7 (HHV-7) in vitro. The antigen positive rate to HHV-6 started to increase 7 days after the infection and reached a maximum by Day 15 using an immunofluorescence antibody test. The copy number of HHV-6 DNA also increased in the samples in 10 days after infection in vitro. No antigen or increase in DNA was detected in PBMCs, that were mock-infected or infected with supernatant of stock virus after ultracentrifugation, suggesting that an infection by HHV-7 is necessary to reactivate HHV-6. In the paired sera samples during the acute and the convalescent phases of ES, seven to ten bands, that were specific for HHV-6, were recognized in samples from the acute phase, and at least 5 dominant polypeptides were found more intensively after HHV-7 infection.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Tanaka-Taya K,Kondo T,Nakagawa N,Inagi R,Miyoshi H,Sunagawa T,Okada S,Yamanishi Kdoi
10.1002/(sici)1096-9071(200003)60:3<284::aid-jmv6>keywords:
subject
Has Abstractpub_date
2000-03-01 00:00:00pages
284-9issue
3eissn
0146-6615issn
1096-9071pii
10.1002/(SICI)1096-9071(200003)60:3<284::AID-JMV6>journal_volume
60pub_type
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