Sequence variation within the human immunodeficiency virus V3 loop at seroconversion.

Abstract:

:Analysis of the HIV-1 V3 quasispecies present in an individual at the time of seroconversion was carried out. The polymerase chain reaction (PCR) was used to amplify proviral HIV-1 DNA extracted from peripheral blood mononuclear cells from a patient who was viraemic (p24 = 15 pg/ml) and had an equivocal HIV-1 antibody status. The PCR products were cloned and the DNA sequence determined for 15 clones. These data showed that the V3 region contained only limited sequence heterogeneity with a major variant accounting for 66% of the protein quasispecies present. The protein sequence of the principal neutralising domain on all species contained the relatively rare GPGKTL motif rather than GPGRAF. The relevance of these data for early stages of HIV infection are discussed.

journal_name

J Med Virol

authors

Ait-Khaled M,Emery VC

doi

10.1002/jmv.1890410403

subject

Has Abstract

pub_date

1993-12-01 00:00:00

pages

270-4

issue

4

eissn

0146-6615

issn

1096-9071

journal_volume

41

pub_type

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