Induction of interleukin 6 impairs the anti-HBV efficiency of IFN-α in human hepatocytes through upregulation of SOCS3.


:Interleukin 6 (IL-6) is a pleiotropic cytokine with pivotal functions in the regulation of the biological responses of several target cells, including hepatocytes. Previous studies have shown that serum IL-6 levels are increased in hepatitis B patients. However, the role of IL-6 in modulating the anti-hepatitis B virus (HBV) activity of interferon-α (IFN-α) remains unclear. In this study, we found that both HBV and viral proteins could induce the expression of IL-6 in hepatocytes (LO2 and HepG2). Exogenous IL-6 had no effect on HBV replication, whereas knockdown of IL-6 expression by RNAi inhibited that. Interestingly, IFN-α markedly induced IL-6 expression in hepatocytes, especially in HBV replicating hepatocytes. In turn, IL-6 impaired the anti-HBV efficiency of IFN-α by decreases the expression of IFN-α downstream effectors by upregulation of suppressor of cytokine signaling-3 (SOCS3). Furthermore, we demonstrated that downregulation of SOCS3 improved IFN antiviral activity to some extent in HBV replicating hepatocytes. These data provided new insights for a better understanding of the mechanism of IFN-α resistance and may represent a novel therapeutic strategy to efficiently target HBV infection.


J Med Virol


Yang K,Guan S,Zhang H,Chen Z




Has Abstract


2019-05-01 00:00:00












  • Epidemiological features and viral shedding in children with SARS-CoV-2 infection.

    abstract::A pandemic of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection broke out all over the world; however, epidemiological data and viral shedding in pediatric patients are limited. We conducted a retrospective, multicenter study, and followed-up with all children from the families with SARS-CoV-2 inf...

    journal_title:Journal of medical virology

    pub_type: 杂志文章,多中心研究


    authors: Hua CZ,Miao ZP,Zheng JS,Huang Q,Sun QF,Lu HP,Su FF,Wang WH,Huang LP,Chen DQ,Xu ZW,Ji LD,Zhang HP,Yang XW,Li MH,Mao YY,Ying MZ,Ye S,Shu Q,Chen EF,Liang JF,Wang W,Chen ZM,Li W,Fu JF

    更新日期:2020-11-01 00:00:00

  • Monoclonal antibody recognizing pre-S(2) epitope of hepatitis B virus: characterization of pre-S(2) epitope and anti-pre-S(2) antibody.

    abstract::A hybrid cell line producing monoclonal antibodies recognizing an epitope encoded by the pre-(S)2 region of hepatitis B virus (HBV) genome was obtained by fusion of mouse myeloma cells with lymphocytes from mice immunized with HBV. The monoclonal antibody Mo-F124 secreted from the hybrid line reacted with the pre-S(2)...

    journal_title:Journal of medical virology

    pub_type: 杂志文章


    authors: Budkowska A,Riottot MM,Dubreuil P,Lazizi Y,Brechot C,Sobczak E,Petit MA,Pillot J

    更新日期:1986-10-01 00:00:00

  • Measles virus RNA is not detected in inflammatory bowel disease using hybrid capture and reverse transcription followed by the polymerase chain reaction.

    abstract::Recent epidemiological and immunohistochemical studies have indicated a possible link between measles virus and inflammatory bowel disease (IBD). The aim of this study was to use a sensitive and robust method for the detection of measles virus RNA in IBD and control clinical samples. Peripheral blood mononuclear cells...

    journal_title:Journal of medical virology

    pub_type: 杂志文章


    authors: Chadwick N,Bruce IJ,Schepelmann S,Pounder RE,Wakefield AJ

    更新日期:1998-08-01 00:00:00

  • Prevalence and types of drug-resistant variants in Chinese patients with acute hepatitis B.

    abstract::The presence of therapy-associated hepatitis B virus (HBV) variants is the main drawback of antiviral therapy for HBV infection. Moreover, drug-resistant variants are more insensitive to a second agent and more therapy-associated mutations will be present. To apply better nucleos(t)ide analogues (NA) and reduce the oc...

    journal_title:Journal of medical virology

    pub_type: 杂志文章


    authors: Su F,Dai J,Yang S,Jiang X,Cui X,Ning H,Li J,Huang M

    更新日期:2015-09-01 00:00:00

  • Genotyping of human papillomavirus in cervical intraepithelial neoplasia in a high-risk population.

    abstract::Infection with the human papillomavirus (HPV) is responsible for 99.7% of cervical cancers, the second most prevalent neoplasia in women worldwide and the fifth leading cause of death by cancer in this population. In Chile, the incidence rate is 14.4 cases per 100,000 women per year and it is considered a significant ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章


    authors: Ili CG,Brebi P,López J,García P,Leal P,Suarez E,Roa JC

    更新日期:2011-05-01 00:00:00

  • HEV and HAV seroprevalence in men that have sex with men (MSM): An update from Milan, Italy.

    abstract::Hepatitis E virus (HEV) is a feco-orally transmitted pathogen and one of the most common cause of acute hepatitis worldwide. Recent studies in developed countries suggested that a direct human-to-human contact such as for sexually transmitted diseases may play a significant role in the HEV spread. The aim of this stud...

    journal_title:Journal of medical virology

    pub_type: 杂志文章


    authors: Greco L,Uceda Renteria SC,Guarneri D,Orlandi A,Zoccoli A,Benardon S,Cusini M,Lunghi G

    更新日期:2018-08-01 00:00:00

  • Hepatitis C virus: buoyant density of the factor VIII-derived isolate in sucrose.

    abstract::Physicochemical and molecular characterization studies of hepatitis C virus (HCV), the major causative agent of parenterally transmitted non-A, non-B hepatitis (PT-NANBH), strongly suggest that it is a pesti-/flavivirus-like virus. Additional studies show that the buoyant density of plasma-derived HCV in sucrose is si...

    journal_title:Journal of medical virology

    pub_type: 杂志文章


    authors: Bradley D,McCaustland K,Krawczynski K,Spelbring J,Humphrey C,Cook EH

    更新日期:1991-07-01 00:00:00

  • Personal clothing as a potential vector of respiratory virus transmission in childcare settings.

    abstract::Previous investigations of fomite transmission have focused on the presence of pathogens on inanimate objects in clinical settings. There has been limited investigation of fomite transmission in non-clinical pediatric settings where there is a high prevalence of respiratory virus infections. Over a 5 week period, this...

    journal_title:Journal of medical virology

    pub_type: 杂志文章


    authors: Gralton J,McLaws ML,Rawlinson WD

    更新日期:2015-06-01 00:00:00

  • A community prevalence study of antibodies to hepatitis A and E in inner-city London.

    abstract::The seroprevalence of antibodies to hepatitis E virus (HEV) and hepatitis A virus (HAV) was determined in a community-based sample in innercity London where socioeconomic conditions were expected to result in a high prevalence of antibodies to HAV, and in which the presence of immigrants from the developing world pose...

    journal_title:Journal of medical virology

    pub_type: 杂志文章


    authors: Bernal W,Smith HM,Williams R

    更新日期:1996-07-01 00:00:00

  • Propagation of hepatitis A virus in human embryo fibroblasts.

    abstract::human diploid fibroblasts and human primary liver cell carcinoma cells (PLC/PRF/5) were infected with hepatitis A virus (HAV) adapted to growth in cell culture or derived directly from human stool. Viral antigen was expressed in PLC/PRF/5 cells 28 days after infection with cell culture-adapted HAV, and 50 days after i...

    journal_title:Journal of medical virology

    pub_type: 杂志文章


    authors: Gauss-Müller V,Frösner GG,Deinhardt F

    更新日期:1981-01-01 00:00:00

  • Prevalence of human parechoviruses in central nervous system infections in children: a retrospective study in Shanghai, China.

    abstract::Human parechoviruses (HPeV) are associated with central nervous system (CNS) infections and sepsis-like illnesses. However, data from China are not available. The aim of this study was to determine the prevalence, age, and seasonal distributions and genotypes of HPeV infections in children with CNS related disease in ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章


    authors: Zhong H,Lin Y,Su L,Cao L,Xu M,Xu J

    更新日期:2013-02-01 00:00:00

  • Identification of epitopes in the nucleocapsid protein of Nipah virus using a linear phage-displayed random peptide library.

    abstract::A random peptide library of heptamers displayed on the surface of M13 bacteriophage was used to identify specific epitopes of antibodies in pooled sera of swine naturally infected by Nipah virus. The selected heptapeptides were aligned with protein sequences of Nipah virus and several putative epitopes were identified...

    journal_title:Journal of medical virology

    pub_type: 杂志文章


    authors: Eshaghi M,Tan WS,Yusoff K

    更新日期:2005-01-01 00:00:00

  • Clinical and immunological investigation of a new combined hepatitis A and hepatitis B vaccine.

    abstract::As with hepatitis B vaccines, the recently developed hepatitis A vaccine is suitable not only for individual protection, but also for public health control measures. For introduction into routine immunisation programmes, however, hepatitis A vaccine should preferably be combined with other already established vaccines...

    journal_title:Journal of medical virology

    pub_type: 临床试验,杂志文章


    authors: Ambrosch F,Wiedermann G,André FE,Delem A,Gregor H,Hofmann H,D'Hondt E,Kundi M,Wynen J,Kunz C

    更新日期:1994-12-01 00:00:00

  • Benefits of random-priming: exhaustive survey of a cDNA library from lung tissue of a SARS patient.

    abstract::The severe acute respiratory syndrome (SARS) leads to severe injury in the lungs with multiple factors, though the pathogenesis is still largely unclear. This paper describes the particular analyses of the transcriptome of human lung tissue that was infected by SARS-associated coronavirus (SARS-CoV). Random primers we...

    journal_title:Journal of medical virology

    pub_type: 杂志文章


    authors: Wu H,Wang J,Deng R,Xing K,Xiong Y,Huang J,He X,Wang X

    更新日期:2011-04-01 00:00:00

  • Nucleotide sequence of hepatitis B virus isolated from subjects without serum anti-hepatitis B core antibody.

    abstract::The nucleotide sequences of the precore/core and X open reading frames (ORFs) of hepatitis B virus (HBV) were studied in four subjects who were serologically negative for anti-hepatitis B core antibody. These subjects were positive for serum hepatitis B surface antigen and were considered to be asymptomatic HBV carrie...

    journal_title:Journal of medical virology

    pub_type: 杂志文章


    authors: Gotoh K,Mima S,Uchida T,Shikata T,Yoshizawa K,Irie M,Mizui M

    更新日期:1995-07-01 00:00:00

  • Laboratory confirmation of congenital rubella syndrome in infants: an eye hospital based investigation.

    abstract::A total of 190 specimens from South Indian children aged 0-59 months with ocular anomalies consistent with suspected congenital rubella syndrome (CRS) were investigated. Twenty-six of the 65 infants (40%) were confirmed as CRS by detection of rubella specific IgM. Rubella RNA was detected in 41 samples from 26 infants...

    journal_title:Journal of medical virology

    pub_type: 杂志文章


    authors: Rajasundari TA,Sundaresan P,Vijayalakshmi P,Brown DW,Jin L

    更新日期:2008-03-01 00:00:00

  • Intestinal immunity to poliovirus develops only after repeated infections in monkeys.

    abstract::To establish gut immunity in monkeys to polio-virus type 1, we fed four juvenile bonnet monkeys (Macaca radiata) with 100 median infectious doses of the Mahoney strain of virus. The duration of viral shedding in the throat and faeces and the titre of virus in faeces were measured. Eighteen days after the last monkey s...

    journal_title:Journal of medical virology

    pub_type: 杂志文章


    authors: Selvakumar R,John TJ

    更新日期:1989-02-01 00:00:00

  • Respiratory disease caused by a species B2 adenovirus in a military camp in Turkey.

    abstract::In April 2004, two patients were admitted to hospital in Berlin, Germany, with clinical signs of acute respiratory infection after returning from a military exercise in their home country of Turkey. They were admitted to a high security infectious disease unit as epidemiological data pointed to an outbreak of unknown ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章


    authors: Chmielewicz B,Benzler J,Pauli G,Krause G,Bergmann F,Schweiger B

    更新日期:2005-10-01 00:00:00

  • A case of Coxsackie A9 virus infection with orchitis.

    abstract::A thirteen-year-old boy with bilateral orchitis was diagnosed as being infected by Coxsackie A9 virus by isolation of the virus from a throat swab and a fecal specimen. Serologic investigations revealed the development of transitory specific neutralizing IgM antibodies against the isotate and of persistent antibodies ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章


    authors: Willems WR,Hornig C,Bauer H,Klingmüller V

    更新日期:1978-01-01 00:00:00

  • Hepatitis C viraemia rebound after "successful" interferon therapy in patients with chronic non-A, non-B hepatitis.

    abstract::A quantitative polymerase chain reaction (PCR) assay for hepatitis C viral RNA (HCV-RNA) was used to monitor viraemia levels in six patients at multiple time points before, during, and after interferon therapy for chronic non-A, non-B hepatitis (NANBH). Prior to therapy, serum HCV-RNA was detected in all patients at a...

    journal_title:Journal of medical virology

    pub_type: 杂志文章


    authors: Garson JA,Brillanti S,Ring C,Perini P,Miglioli M,Barbara L

    更新日期:1992-07-01 00:00:00

  • Effect of age on serum immunoglobulin G subclass antibody responses to inactivated influenza virus vaccine.

    abstract::We previously reported an age-associated impairment of serum immunoglobulin G (IgG) antibody responses to inactivated influenza virus vaccine. The present study extends these observations by examining the IgG subclass distribution of vaccine responses measured by enzyme linked immunosorbent assay in healthy adults age...

    journal_title:Journal of medical virology

    pub_type: 杂志文章


    authors: Powers DC

    更新日期:1994-05-01 00:00:00

  • Immunogenicity of wild and attenuated varicella-zoster virus strains in rhesus monkeys.

    abstract::Thirty susceptible rhesus monkeys were inoculated with cell-free varicella-zoster virus strain OKA or strain KMcC. Both wild and attenuated strains were used. No clinical signs characteristic of human varicella were seen in any of the animals. Virus was not isolated from throat swabs, blood, or cerebrospinal fluid. An...

    journal_title:Journal of medical virology

    pub_type: 杂志文章


    authors: Asano Y,Albrecht P,Behr DE,Neff BJ,Vickers JH,Rastogi SC

    更新日期:1984-01-01 00:00:00

  • HBeAg-negative chronic hepatitis patients should be monitored more strictly: a cross-sectional retrospective study on antiviral treatment-naïve patients.

    abstract:AIM:To investigate the prevalence and characteristics of hepatitis B e antigen (HBeAg) negative/treatment naïve subjects with low hepatitis B virus (HBV) DNA levels (<10(4)  copies/ml) and low alanine aminotransferase (ALT) levels (<2 × upper limit of normal) in patients with HBV-related hepatocellular carcinoma (HCC)....

    journal_title:Journal of medical virology

    pub_type: 杂志文章


    authors: Zeng DW,Dong J,Zhang JM,Zhu YY,Jiang JJ,Liu YR

    更新日期:2015-10-01 00:00:00

  • Development of a sensitive assay for the measurement of antibodies against heat shock protein binding protein 1 (HspBP1): increased levels of anti-HspBP1 IgG are prevalent in HIV infected subjects.

    abstract::The 70 kDa heat shock protein (Hsp70) is generally considered to be an intracellular protein, however, there is evidence that Hsp70 can be found in the extracellular environment. Hsp70 and antibodies against Hsp70 have been reported in human serum. Recent evidence has shown that Hsp70 antibodies are elevated in HIV in...

    journal_title:Journal of medical virology

    pub_type: 杂志文章


    authors: Papp D,Prohászka Z,Kocsis J,Füst G,Bánhegyi D,Raynes DA,Guerriero V

    更新日期:2005-08-01 00:00:00

  • Sequence-specific detection method for reverse transcription, loop-mediated isothermal amplification of HIV-1.

    abstract::HIV diagnosis at the point-of-care or in resource-limited settings poses considerable challenges due to time and cost limitations. Currently, nucleic acid-based tests are the only reliable method for diagnosing recent infections during the window period post-infection and pre-seroconversion, but these tests are only s...

    journal_title:Journal of medical virology

    pub_type: 杂志文章


    authors: Curtis KA,Rudolph DL,Owen SM

    更新日期:2009-06-01 00:00:00

  • Herpesvirus detection and cytokine levels (IL-10, IL-6, and IFN-γ) in ocular fluid from Tunisian immunocompetent patients with uveitis.

    abstract::Human herpesviruses (HHVs) are involved in the pathogenesis of different types of uveitis. Cytokine response plays an important role in virus-induced immunopathology. This study aimed to investigate the incidence of HHVs in aqueous humor samples of immunocompetent patients with suspected viral uveitis and cytokine (IL...

    journal_title:Journal of medical virology

    pub_type: 杂志文章


    authors: Nahdi I,Abdelwahed RB,Boukoum H,Bressollette-Bodin C,Attia S,Yahia SB,Fisson S,Khairallah M,Aouni M

    更新日期:2013-12-01 00:00:00

  • Seroprevalence rates of HPyV6, HPyV7, TSPyV, HPyV9, MWPyV and KIPyV polyomaviruses among the healthy blood donors.

    abstract::Human polyomaviruses HPyV6, HPyV7, TSPyV, HPyV9, MWPyV, and KIPyV have been discovered between 2007 and 2012. TSPyV causes a rare skin disease trichodysplasia spinulosa in immunocompromised patients, the role of remaining polyomaviruses in human pathology is not clear. In this study, we assessed the occurrence of seru...

    journal_title:Journal of medical virology

    pub_type: 杂志文章


    authors: Šroller V,Hamšíková E,Ludvíková V,Musil J,Němečková Š,Saláková M

    更新日期:2016-07-01 00:00:00

  • Impact of HIV infection and highly active antiretroviral therapy (HAART) on B cell subpopulations in children.

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    journal_title:Journal of medical virology

    pub_type: 杂志文章


    authors: Singh R,Mukherjee A,Singla M,Vajpayee M,Negi N,Kabra SK,Lodha R,Das BK

    更新日期:2018-07-01 00:00:00

  • Latent membrane protein 1 encoded by Epstein-Barr virus induces telomerase activity via p16INK4A/Rb/E2F1 and JNK signaling pathways.

    abstract::Elevated telomerase activity is observed in about 90% of human cancers. This activity correlates strictly with human telomerase reverse transcriptase (hTERT). Previously, it was shown that the Epstein-Barr virus-encoded latent membrane protein 1 (LMP1) induced telomerase activity in nasopharyngeal carcinoma cells. In ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章


    authors: Ding L,Li L,Yang J,Zhou S,Li W,Tang M,Shi Y,Yi W,Cao Y

    更新日期:2007-08-01 00:00:00

  • Detection of integrated hepatitis B virus DNA in hepatocellular carcinoma cell lines by nonradioactive in situ hybridization.

    abstract::A sensitive and specific nonradioactive in situ hybridization method capable of detecting single-copy integrated hepatitis B virus (HBV) DNA sequences in hepatocellular carcinoma (HCC) cell lines was developed. In situ hybridization of biotinylated HBV (adr, adw) DNA probes with nine different human HCC cell lines wer...

    journal_title:Journal of medical virology

    pub_type: 杂志文章


    authors: Tay N,Chan SH,Ren EC

    更新日期:1990-04-01 00:00:00