Standardization of a plaque assay for Lassa virus.

abstract：:A thirteen-year-old boy with bilateral orchitis was diagnosed as being infected by Coxsackie A9 virus by isolation of the virus from a throat swab and a fecal specimen. Serologic investigations revealed the development of transitory specific neutralizing IgM antibodies against the isotate and of persistent antibodies ...

journal_title：Journal of medical virology

authors： Willems WR,Hornig C,Bauer H,Klingmüller V

更新日期：1978-01-01 00:00:00

abstract：:A small percentage of renal patients become infected with the BK polyomavirus (BKV), a pathogenic virus that causes BKV-associated nephropathy (BKVN), after kidney transplantation. This study presents a simple, rapid, high-throughput method for BKV genotyping using high-resolution melt analysis (HRMA). Using this nove...

journal_title：Journal of medical virology

authors： Matsuda Y,Qazi Y,Iwaki Y

更新日期：2011-12-01 00:00:00

abstract：:Chikungunya fever is a viral disease transmitted to humans by the bite of infected mosquitoes. The disease is characterized by fever, headache, rash, severe joint, and muscle pain. To evaluate the disease burden in the population and the effectiveness of public health measures, periodic seroprevalence surveys are esse...

journal_title：Journal of medical virology

authors： Shilpa C,Kavitha K,Sudheesh N,Sabeena S,Prasad V,Hindol M,Arunkumar G

更新日期：2020-01-01 00:00:00

abstract：:Dengue, caused by any of the four types of Dengue virus (DENV) is the most important arbovirus in the world. In this study we performed a molecular surveillance of dengue during the greatest dengue outbreak that took place in Divinópolis, Minas Gerais state, Southeast Brazil, in 2013. Samples from 100 patients with cl...

journal_title：Journal of medical virology

authors： Dutra KR,Drumond BP,de Rezende IM,Nogueira ML,de Oliveira Lopes D,Calzavara Silva CE,Siqueira Ferreira JM,Dos Santos LL

更新日期：2017-06-01 00:00:00

abstract：:BK polyomavirus (BKV) is a ubiquitous virus in humans that remains latent in the urogenital tract after a primary infection during childhood. The virus, which is reactivated frequently and excreted in urine, can cause nephropathy in renal transplant recipients. BKV sequences are classified into four subtypes (I-IV). S...

journal_title：Journal of medical virology

authors： Boukoum H,Nahdi I,Foulongne V,Zallema D,Aloui S,Achour A,Segondy M,Aouni M

更新日期：2011-04-01 00:00:00

abstract：:Serum levels of interleukin-6 (IL-6) are elevated in acute and chronic hepatitis B patients. The effect of IL-6 and its transcription factor of NF-IL6 (a nuclear factor for IL-6) on hepatitis B virus (HBV) enhancer 1 (Enh1), which controls HBV X expression, were investigated in HepG2 cells. Twenty ng/ml of IL-6 increa...

journal_title：Journal of medical virology

authors： Ohno H,Kaneko S,Kobayashi K,Murakami S

更新日期：1997-08-01 00:00:00

abstract：:During the period between 1998 and 2008, 48 representative measles viruses (MeVs) circulating in Thailand were subjected to genetic characterization. Three genotypes, G2, D5, and D9 were detected. The results suggested that measles genotype D5, which has been circulating since at least 1998, is the endemic genotype in...

journal_title：Journal of medical virology

authors： Pattamadilok S,Incomserb P,Primsirikunawut A,Lukebua A,Rota PA,Sawanpanyalert P

更新日期：2012-05-01 00:00:00

abstract：:Dengue virus (DV) infection can result in either a mild febrile illness known as dengue fever (DF) or a life-threatening disease called dengue hemorrhagic fever (DHF). DHF is more prevalent in patients undergoing secondary DV infection. This observation has led to the hypothesis that DHF may be the result of immune re...

journal_title：Journal of medical virology

authors： Spain-Santana TA,Marglin S,Ennis FA,Rothman AL

更新日期：2001-10-01 00:00:00

abstract：:Because of the distinct ability of retroviruses to integrate into the target cell genome and thus achieve long-term expression, retrovirus vectors hold great promise for stable gene transfer. Such vectors derived from human immunodeficiency retroviruses (HIVs) and other lentiviruses are envisioned to possess several a...

journal_title：Journal of medical virology

authors： Sadaie MR,Zamani M,Whang S,Sistron N,Arya SK

更新日期：1998-02-01 00:00:00

abstract：:The purpose of this study was to investigate determinants of endemic hepatitis C virus (HCV) infection within communities in Taiwan. A two-phase study, including a seroprevalence survey and a prevalent case-control study at the first phase, which has been published previously, and a follow-up seroconversion determinat...

journal_title：Journal of medical virology

authors： Sun CA,Chen HC,Lu SN,Chen CJ,Lu CF,You SL,Lin SH

更新日期：2001-09-01 00:00:00

abstract：:Mitochondrial DNA (mtDNA) of peripheral blood mononuclear cells (PBMCs) collected from Human immunodeficiency virus 1 (HIV-1)-infected patients and healthy controls were measured longitudinally using real-time polymerase chain reaction to evaluate the effects of antiretroviral agents on mtDNA synthesis in vivo and to ...

journal_title：Journal of medical virology

authors： Miura T,Goto M,Hosoya N,Odawara T,Kitamura Y,Nakamura T,Iwamoto A

更新日期：2003-08-01 00:00:00

abstract：:In order to shed light on the mode of HTLV-I infection by mother-to-child transmission, we examined sera of school children in a highly endemic town on two separate occasions at a 6-year interval. The carrier rates in ages 15-17, 8.7 and 2.1%, were significantly higher than that in ages 6-8, 1.7 and 0.4%, in studies. ...

journal_title：Journal of medical virology

authors： Sugiyama H,Doi H,Yamaguchi K,Tsuji Y,Miyamoto T,Hino S

更新日期：1986-11-01 00:00:00

abstract：:In vitro incubation of human cytomegalovirus (Towne strain) with 8 U/ml human recombinant myeloperoxidase plus sodium chloride and glucose nearly abolished viral infectivity. To assay the effect on intracellular infection, cell toxicity of the enzymes was first studied. Even the high dose of 16 U/ml of recombinant mye...

journal_title：Journal of medical virology

authors： El Messaoudi K,Verheyden AM,Thiry L,Fourez S,Tasiaux N,Bollen A,Moguilevsky N

更新日期：2002-02-01 00:00:00

abstract：:A modified centrifugation culture technique and a polymerase chain reaction (PCR) is described for detection of early antigen and IE antigen DNA, respectively, for rapid and sensitive monitoring of active cytomegalovirus (HCMV) infection after organ transplantation. In a preliminary study, 541 clinical specimens (bloo...

journal_title：Journal of medical virology

authors： Prösch S,Kimel V,Dawydowa I,Krüger DH

更新日期：1992-12-01 00:00:00

abstract：:An efficient method for cloning subgenomic fragments of the hepatitis B virus (HBV) was developed, utilizing its abundant single-stranded replicative intermediates. The total genomic DNA obtained from the liver tissue of patients with chronic HBV infection was treated by using the Klenow fragment of E. coli DNA polyme...

journal_title：Journal of medical virology

authors： Kajino K,Ohta Y,Miyamura T,Saito I

更新日期：1991-01-01 00:00:00

abstract：:Parechoviruses are emerging pathogens of humans often affecting the pediatric age group, with a growing line of evidence implicating them as agents of a broad spectrum of clinical syndromes in adults. However, because many clinicians are not familiar with the manifestation of the infections, they are not included in t...

journal_title：Journal of medical virology

authors： Kabuga AI,Nejati A,Soheili P,Shahmahmoodi S

更新日期：2020-08-06 00:00:00

abstract：:Foscarnet (trisodium phosphonoformate) is a novel antiviral agent that inhibits viral-specific DNA polymerase. In the present study, eight males with chronic HBV carriage (HBeAg and HBV-DNA seropositivity greater than 12 months) showing chronic persistent hepatitis (CPH) or chronic active hepatitis (CAH) on liver biop...

journal_title：Journal of medical virology

authors： Bain VG,Daniels HM,Chanas A,Alexander GJ,Williams R

更新日期：1989-10-01 00:00:00

abstract：:Many patients with coagulation disorders are infected with hepatitis C virus (HCV) that advances to end stage liver disease, resulting in an increased number of deaths. The efficacy of ribavirin and peginterferon combination therapy for chronic HCV infection in patients with coagulation disorders has not been clarifie...

journal_title：Journal of medical virology

authors： Honda T,Katano Y,Kuzuya T,Hayashi K,Ishigami M,Itoh A,Hirooka Y,Nakano I,Ishikawa T,Toyoda H,Kumada T,Yamamoto K,Matsushita T,Kojima T,Takamatsu J,Goto H

更新日期：2013-02-01 00:00:00

abstract：:Hepatitis E virus (HEV), the sole member of the genus Hepevirus in the family of Hepeviridae, is the major cause of several outbreaks of waterborne hepatitis in tropical and subtropical countries and of sporadic cases of viral hepatitis in endemic and industrialized countries. Transmission of HEV occurs predominantly ...

journal_title：Journal of medical virology

authors： Mushahwar IK

更新日期：2008-04-01 00:00:00

abstract：:Chitin in the form of microparticles (chitin microparticles, CMP) has been demonstrated to be a potent stimulator of macrophages, promoting T-helper-1 (Th1) activation and cytokine response. In order to examine the mucosal adjuvant effect of CMP co-administered with influenza hemagglutinin (HA) vaccine against influen...

journal_title：Journal of medical virology

authors： Hasegawa H,Ichinohe T,Strong P,Watanabe I,Ito S,Tamura S,Takahashi H,Sawa H,Chiba J,Kurata T,Sata T

更新日期：2005-01-01 00:00:00

abstract：:The aim of the study was to evaluate the prevalence and risk factors of HPV in a gynecologic population attending outpatient clinics using two new molecular tests. The Amplicor HPV test and the Linear Array (LA) HPV Genotyping test were used for the detection of HPV DNA in 320 women. Multiple logistic regression was u...

journal_title：Journal of medical virology

authors： Papachristou E,Sypsa V,Paraskevis D,Gkekas A,Politi E,Nicolaidou E,Anifantis I,Psichogiou M,Tsitsika A,Hadjivassiliou M,Petrikkos G,Katsambas A,Creatsas G,Hatzakis A

更新日期：2009-12-01 00:00:00

abstract：:A sensitive and reproducible real-time PCR assay based on TaqMan technology was developed for the detection and quantitation of hepatitis B virus (HBV) DNA in serum, and compared with an "in-house" qualitative PCR assay. HBV DNA was measured in 125 serum samples from 76 hepatitis B patients, consisting of 22 patients ...

journal_title：Journal of medical virology

authors： Chen RW,Piiparinen H,Seppänen M,Koskela P,Sarna S,Lappalainen M

更新日期：2001-10-01 00:00:00

abstract：:Flavivirus infections are a serious healthcare concern in tropical and subtropical countries. Although well-established laboratory tests can provide early diagnosis of acute dengue or Zika infections, access to these tests is limited in developing countries, presenting an urgent need to develop simple, rapid, and robu...

journal_title：Journal of medical virology

authors： Bedin F,Boulet L,Voilin E,Theillet G,Rubens A,Rozand C

更新日期：2017-09-01 00:00:00

abstract：:A real-time PCR assay was developed for quantitative detection of B19 DNA in clinical serum samples. The assay was carried out using a LightCycler instrument and product formation was monitored continuously with the fluorescent double-stranded DNA binding dye SYBR Green I. With an optimized PCR protocol, this system w...

journal_title：Journal of medical virology

authors： Manaresi E,Gallinella G,Zuffi E,Bonvicini F,Zerbini M,Musiani M

更新日期：2002-06-01 00:00:00

abstract：:This was an exploratory study to investigate lamivudine-resistant hepatitis B virus (HBV) strains in selected lamivudine-naïve HBV carriers with and without human immunodeficiency virus (HIV) co-infection in South African patients. Thirty-five lamivudine-naïve HBV infected patients with or without HIV co-infection wer...

journal_title：Journal of medical virology

authors： Selabe SG,Lukhwareni A,Song E,Leeuw YG,Burnett RJ,Mphahlele MJ

更新日期：2007-11-01 00:00:00

abstract：:N-Acetylethyleneimine (AEI) was used to inactivate the avirulent Togavirus Semliki Forest virus (A774 strain) grown in chick embryo, Vero, and brain cell cultures. The purity of the virus preparation affected the kinetics of inactivation. The rate of inactivation increased with a rise in temperature from 5 to 40 degre...

journal_title：Journal of medical virology

authors： Amor S,Webb HE

更新日期：1986-08-01 00:00:00

abstract：:The nature of the immunodominant a region of hepatitis B virus surface antigen (HBsAg) has been examined by mutation of specific amino acid residues. Proline 142 was required for the exhibition of full antigenicity. Replacement of cysteines 124 and 147 by serines drastically reduced or eliminated reactivity with antib...

journal_title：Journal of medical virology

authors： Ashton-Rickardt PG,Murray K

更新日期：1989-11-01 00:00:00

abstract：:This study aimed to examine the application of hepatitis B surface antigen (HBsAg) confirmatory testing when diagnosing hepatitis B infection among young persons in Taiwan with a low prevalence rate of hepatitis B infection. HBsAg status, the presence of antibodies against HBsAg (anti-HBs), and the presence of antibod...

journal_title：Journal of medical virology

authors： Chu FY,Su FH,Cheng SH,Lin YS,Li CY,Chien CC,Lin YC,Chiang SY

更新日期：2011-09-01 00:00:00

abstract：:The evaluation of new therapeutic resources against coronavirus disease 2019 (COVID-19) represents a priority in clinical research considering the minimal options currently available. To evaluate the adjuvant use of systemic oxygen-ozone administration in the early control of disease progression in patients with COVID...

journal_title：Journal of medical virology

authors： Araimo F,Imperiale C,Tordiglione P,Ceccarelli G,Borrazzo C,Alessandri F,Santinelli L,Innocenti GP,Pinacchio C,Mauro V,Recchia GE,Zancla S,Calò A,Poscia R,Ruberto F,d'Ettorre G,Bilotta F,Mastroianni C,Pugliese F

更新日期：2020-10-28 00:00:00

abstract：:Hepatitis E is endemic in India. It was recently noted that although all the Indian human hepatitis E virus (HEV) isolates (1976-2001) were placed in genotype I, the swine HEV recovered from western India (2000) belonged to genotype IV. This was in contrast to reports from the United States and Taiwan wherein both hum...

journal_title：Journal of medical virology

authors： Arankalle VA,Chobe LP,Walimbe AM,Yergolkar PN,Jacob GP

更新日期：2003-03-01 00:00:00