Monitoring of patients for cytomegalovirus after organ transplantation by centrifugation culture and PCR.

Abstract:

:A modified centrifugation culture technique and a polymerase chain reaction (PCR) is described for detection of early antigen and IE antigen DNA, respectively, for rapid and sensitive monitoring of active cytomegalovirus (HCMV) infection after organ transplantation. In a preliminary study, 541 clinical specimens (blood, urine, bronchoalveolar lavage, pharyngeal wash, sputum) from 59 organ recipients were assayed for HCMV antigen by centrifugation culture; 144 samples were tested by PCR simultaneously. Antigenemia detected by centrifugation culture correlated strongly with active HCMV infection and clinical symptoms and proved useful for monitoring the efficacy of antiviral therapy. PCR was more sensitive in an earlier phase of infection when centrifugation culture was still negative. The clinical usefulness of both methods is discussed.

journal_name

J Med Virol

authors

Prösch S,Kimel V,Dawydowa I,Krüger DH

doi

10.1002/jmv.1890380404

keywords:

subject

Has Abstract

pub_date

1992-12-01 00:00:00

pages

246-51

issue

4

eissn

0146-6615

issn

1096-9071

journal_volume

38

pub_type

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