Abstract:
:A modified centrifugation culture technique and a polymerase chain reaction (PCR) is described for detection of early antigen and IE antigen DNA, respectively, for rapid and sensitive monitoring of active cytomegalovirus (HCMV) infection after organ transplantation. In a preliminary study, 541 clinical specimens (blood, urine, bronchoalveolar lavage, pharyngeal wash, sputum) from 59 organ recipients were assayed for HCMV antigen by centrifugation culture; 144 samples were tested by PCR simultaneously. Antigenemia detected by centrifugation culture correlated strongly with active HCMV infection and clinical symptoms and proved useful for monitoring the efficacy of antiviral therapy. PCR was more sensitive in an earlier phase of infection when centrifugation culture was still negative. The clinical usefulness of both methods is discussed.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Prösch S,Kimel V,Dawydowa I,Krüger DHdoi
10.1002/jmv.1890380404keywords:
subject
Has Abstractpub_date
1992-12-01 00:00:00pages
246-51issue
4eissn
0146-6615issn
1096-9071journal_volume
38pub_type
杂志文章abstract::Hepatitis E virus (HEV) infection has become a global concern with high mortality rates among pregnant women, especially those in their third trimester of pregnancy. Estrogen plays an important role in mediating the body, regulating physiological and pathological processes. Estrogen is activated by binding to estrogen...
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journal_title:Journal of medical virology
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journal_title:Journal of medical virology
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journal_title:Journal of medical virology
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journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
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abstract::The role of Epstein-Barr virus (EBV) in the pathogenesis of gastric lymphoma of mucosa-associated lymphoid tissue (MALT) has not been well understood. The aim of the study was to investigate EBV infection and its gene expression in this tumor in order to understand its role in the pathogenesis. EBV infection was scree...
journal_title:Journal of medical virology
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doi:10.1002/(sici)1096-9071(199812)56:4<342::aid-jmv10
更新日期:1998-12-01 00:00:00
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更新日期:2006-04-01 00:00:00
abstract::Knowledge of the precise timing of SARS-CoV-2 infection may be of clinical and epidemiological relevance. The presence of low-avidity IgGs has conventionally been considered an indicator of recent infection. Here, we carried out qualitative assessment of SARS-CoV-2-specific antibody avidity using an urea (6M) dissocia...
journal_title:Journal of medical virology
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更新日期:2021-02-01 00:00:00
abstract::The effect of antiviral treatment on apoptosis in chronic hepatitis B (CHB) has not been clarified. We evaluated the hepatic immunohistochemical expression of the pro-apoptotic bax and the antiapoptotic bcl-xL protein in HBeAg-negative CHB patients before and after treatment. In our study we included 72 paired biopsie...
journal_title:Journal of medical virology
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abstract::The quantification of human immunodeficiency virus type 1 (HIV-1) RNA or hepatitis C virus (HCV) RNA has been facilitated by adapting a spin column procedure for sample preparation and the use of chemiluminescent detection of polymerase chain reaction (PCR) products in microtiter plate format. All materials were comme...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract::The purpose of this study was to investigate determinants of endemic hepatitis C virus (HCV) infection within communities in Taiwan. A two-phase study, including a seroprevalence survey and a prevalent case-control study at the first phase, which has been published previously, and a follow-up seroconversion determinat...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
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abstract::Hepatitis E virus (HEV), the sole member of the genus Hepevirus in the family of Hepeviridae, is the major cause of several outbreaks of waterborne hepatitis in tropical and subtropical countries and of sporadic cases of viral hepatitis in endemic and industrialized countries. Transmission of HEV occurs predominantly ...
journal_title:Journal of medical virology
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pub_type: 杂志文章
doi:10.1002/jmv.1890210306
更新日期:1987-03-01 00:00:00
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更新日期:1998-03-01 00:00:00
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journal_title:Journal of medical virology
pub_type: 杂志文章,多中心研究,随机对照试验
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更新日期:2012-08-01 00:00:00
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pub_type: 杂志文章,评审
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journal_title:Journal of medical virology
pub_type: 杂志文章
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更新日期:1992-07-01 00:00:00
abstract::The objective of the study was to assess the genotypic and phenotypic properties of 18 viral strains from human immunodeficiency virus-1 (HIV-1) positive patients and to identify subtype C isolates for vaccine design strategies. All the isolates were non-syncytium-inducing (NSI) in both the primary and MT-2 cell cultu...
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更新日期:2002-10-01 00:00:00