Abstract:
:The role of Epstein-Barr virus (EBV) in the pathogenesis of gastric lymphoma of mucosa-associated lymphoid tissue (MALT) has not been well understood. The aim of the study was to investigate EBV infection and its gene expression in this tumor in order to understand its role in the pathogenesis. EBV infection was screened by in situ hybridization for EBV-encoded non-polyadenylated RNA (EBER ISH) in 79 cases of gastric MALT lymphoma of nonimmunocompromised patients. The expression of EBV proteins [LMP1 (latent membrane protein 1), EBNA2 (EBV nuclear antigen 2), ZEBRA (switch protein encoded by BZLF1 gene)] was studied by immunohistochemistry in EBER-positive cases. EBV was detected with EBER ISH in 15 (19%) of the 79 cases. EBV was found in virtually all tumor cells in 2 cases of high-grade MALT lymphoma (2.5%) (EBV-associated), and was found only in occasional large or small lymphoid cells in 13 cases (16.5%). False positive EBER signal was detected in the mucinous glandular epithelial cells of gastric antrum with FITC-labeled oligonucleotide probe but not with digoxigenin or 35S-labeled riboprobes. Type II latency (EBER+LMP1+ EBNA2-) was detected in both EBV-associated cases. Type III latency (EBER+LMP1+EBNA2+) was also identified in one EBV-associated case besides latency II. Double labeling showed coexpression of LMP1 and EBNA2 in a small number of tumor cells, indicating the presence of type III latency in single cell level. In cases with only occasional EBER-positive large or small lymphoid cells, LMP1 and EBNA2 were not detected. ZEBRA was negative in all the cases. These findings suggest that EBV may contribute to the pathogenesis of a small proportion of high-grade MALT lymphoma, where virtually all tumor cells harbored EBV and the oncogenic viral protein LMP1 was expressed. Moreover, latency III of EBV infection may exist in nonimmunocompromised patient.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Xu WS,Chan AC,Lee JM,Liang RH,Ho FC,Srivastava Gdoi
10.1002/(sici)1096-9071(199812)56:4<342::aid-jmv10subject
Has Abstractpub_date
1998-12-01 00:00:00pages
342-50issue
4eissn
0146-6615issn
1096-9071pii
10.1002/(SICI)1096-9071(199812)56:4<342::AID-JMV10journal_volume
56pub_type
杂志文章abstract::The seroprevalence of viral hepatitis in healthcare workers has important public health implications. To assess the risk factors for the acquisition of viral hepatitis in an unvaccinated cohort from an hyperendemic region, 567 healthcare workers from a large hospital in the capital of the Republic of Yemen were interv...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20126
更新日期:2004-08-01 00:00:00
abstract::Hepatitis B virus (HBV) and human immunodeficiency virus (HIV) have similar transmission routes, implying that patients infected with HIV are at particular risk for HBV infection. Patients who are co-infected with HIV and HBV progress more rapidly to end-stage liver disease and different HBV genotypes may have a disti...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.22174
更新日期:2011-11-01 00:00:00
abstract::Epstein-Barr virus (EBV) DNA load in the blood increases in posttransplant lymphoproliferative disorders and chronic active EBV infection. In this report, we analyzed the EBV DNA load in the peripheral blood mononuclear cells (PBMCs) and plasma of patients with hydroa vacciniforme (HV) and/or hypersensitivity to mosqu...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25811
更新日期:2020-04-07 00:00:00
abstract::Coronavirus disease 2019 (COVID-19) can lead to a massive cytokine release. The use of the anti-interleukin-6 receptor monoclonal antibody tocilizumab (TCZ) has been proposed in this hyperinflammatory phase, although supporting evidence is limited. We retrospectively analyzed 88 consecutive patients with COVID-19 pneu...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26308
更新日期:2021-02-01 00:00:00
abstract::A thirteen-year-old boy with bilateral orchitis was diagnosed as being infected by Coxsackie A9 virus by isolation of the virus from a throat swab and a fecal specimen. Serologic investigations revealed the development of transitory specific neutralizing IgM antibodies against the isotate and of persistent antibodies ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890030207
更新日期:1978-01-01 00:00:00
abstract::Anti-influenza serum and nasal antibody titers were followed during a double-blind, placebo-controlled, randomized study assessing the prophylactic efficacy of 50, 100, and 200 mg/day of amantadine hydrochloride against experimental challenge with influenza A/Beth/1/85. The geometric mean titers (GMT) of serum hemaggl...
journal_title:Journal of medical virology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1002/jmv.1890270213
更新日期:1989-02-01 00:00:00
abstract::The effects of 6-diazo-5-oxo-L-norleucine (DON), 2-deoxy-D-glucose (DOG), and tunicamycin (TM) on the replication of poliovirus (PV) and vesicular stomatitis virus (VSV) were examined. During a 48-hr replication period, TM, DON, and DOG inhibit VSV plaque formation in HEp-2 cells by 99.9%, 99.8%, and 99.9% respectivel...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890140210
更新日期:1984-01-01 00:00:00
abstract::Subgroup A respiratory syncytial viruses present in respiratory secretions and low passage level cell culture isolates were found to be markedly less susceptible to neutralization with monoclonal antibodies (MAbs) to the F glycoprotein than the cell culture adapted A2 virus strain. Low passage virus isolates collected...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20892
更新日期:2007-06-01 00:00:00
abstract::Three hepatitis B surface antigen (HBsAg) preparations were compared: purified intact 22-nm HBsAg particles; HBsAg-derived, sodium dodecyl sulfate (SDS)-denatured P25 + GP30 polypeptide pool; and nondenatured P25 + GP30 micelles. The micelles had the same polypeptide composition as the P25 + GP30 pool. The immunogenic...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890110205
更新日期:1983-01-01 00:00:00
abstract::Previous studies on patients who develop drug resistant HIV-1 variants have shown that continued use of failing regimens might provide clinical benefit. However, the effect of long-term exposure to drug resistant variants may lead to emergence of compensatory mutations that may jeopardize this effect. In this study, w...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20582
更新日期:2006-05-01 00:00:00
abstract::WU and KI polyomaviruses (WUPyV, KIPyV) have been detected in respiratory, blood, stool, and lymphoid tissue, but not in urine samples. PCR based detection revealed higher frequency in immunocompromised individuals. In this study the prevalence of WUPyV and KIPyV was analyzed in respiratory, urine, and blood samples f...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.22083
更新日期:2011-07-01 00:00:00
abstract::Fifty-six renal allograft recipients were studied prospectively for 3 months or longer after transplant. The polymerase chain reaction (PCR) was used to screen peripheral blood leucocyte (PBL) specimens for CMV, human herpesvirus 6 (HHV6) and human herpesvirus 7 (HHV7) DNA (DNAemia) in 67 healthy controls and in seria...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(SICI)1096-9071(199604)48:4<295::AID-JMV1>
更新日期:1996-04-01 00:00:00
abstract:BACKGROUND AND AIM:A meta-analysis was performed to identify COVID-19 patients presenting with gastrointestinal (GI) symptoms during the first and second pandemic waves and investigate their association with the disease outcomes. METHODS:A systematic search in PubMed, Scopus, Web of Science, ScienceDirect, and EMBASE ...
journal_title:Journal of medical virology
pub_type: 杂志文章,评审
doi:10.1002/jmv.26836
更新日期:2021-02-02 00:00:00
abstract::Human herpesvirus 8 (HHV-8) is the etiologic agent of Kaposi's sarcoma (KS). Several studies indicate horizontal HHV-8 transmission among children in areas where KS is endemic, but few studies have assessed acquisition of HHV-8 by children in low seroprevalence areas. Antibody screening was carried out for HHV-8 and E...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10548
更新日期:2004-01-01 00:00:00
abstract::This open, randomized study was conducted in healthy Chinese youngsters, aged between 10 and 19 years to compare the reactogenicity and immunogenicity of two vaccines: the combined vaccine against hepatitis A and B was administered in a two-dose schedule with the profile of the corresponding monovalent vaccines, while...
journal_title:Journal of medical virology
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1999-09-01 00:00:00
abstract::Influenza A(H1N1)pdm09 virus pandemic struck India in 2009 and continues to cause outbreaks in its post-pandemic phase. Diminutive information is available about influenza A(H1N1)pdm09 from central India. This observational study presents epidemiological and molecular findings for the period of 6 years. Throat swab sa...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24982
更新日期:2018-03-01 00:00:00
abstract::Hepatitis A infections still represent a major global health concern. During the past years, a transition pattern of the hepatitis A epidemiology was noted in many parts of the world. In Tunisia, there is not a recent survey on age-specific hepatitis A virus seroprevalence. This study aimed to investigate the seroprev...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26563
更新日期:2020-09-28 00:00:00
abstract::Over the past decade, a G4P[6] strain has been found to be circulating in different neonatal wards in the Pretoria area. This endemic strain was associated with both asymptomatic and symptomatic infection, providing the opportunity to undertake a molecular study of some of the putative "virulence" genes. The genes enc...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/1096-9071(200010)62:2<208::aid-jmv12>3.0.c
更新日期:2000-10-01 00:00:00
abstract::Immunocompromised individuals were tested for the presence of the human polyomaviruses JC (JCV) and BK (BKV) by the polymerase chain reaction (PCR). The use of appropriate primers in a nested PCR allowed the detection of both viruses simultaneously. Viruses were differentiated by restriction fragment length analysis o...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890420208
更新日期:1994-02-01 00:00:00
abstract::Human metapneumovirus, with two known genotypes named A and B, is associated with mild respiratory symptoms to severe LRTI in children, high-risk adults and the elderly. Rapid and reliable methods of hMPV detection in clinical samples are essential to implement appropriate care, to better understand the pathology of h...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21038
更新日期:2008-01-01 00:00:00
abstract::Hepatitis A virus (HAV) is a causative agent of acute viral hepatitis, which represents a significant public health problem. HAV is usually transmitted by oral-fecal route and prevalent not only in developing countries but also in developed countries worldwide. To characterize the HAV wild type strains circulating in ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21127
更新日期:2008-05-01 00:00:00
abstract::Although cytomegalovirus (CMV) has been detected in the inner ear fluid of patients who succumbed to the complications of symptomatic congenital CMV infection, it has not been detected in the inner ear fluid of living patients. In this study, real-time polymerase chain reaction (PCR) was used to measure CMV DNA in cli...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10263
更新日期:2003-01-01 00:00:00
abstract::In mid-December 2019, a disease caused by infection with severe acute respiratory syndrome coronavirus-2, which began in Wuhan, China, has spread throughout the country and many countries around the world. The number of children with coronavirus disease-2019 (COVID-19) has also increased significantly. Although inform...
journal_title:Journal of medical virology
pub_type: 杂志文章,评审
doi:10.1002/jmv.25807
更新日期:2020-07-01 00:00:00
abstract::Recent studies have identified epithelial cell populations in human livers that are similar to the "oval cells" and "transitional cells" seen in rat livers during the early stages of chemical carcinogenesis. It has been suggested that these cells might be precursors of hepatocytes and theoretically could be involved i...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890430304
更新日期:1994-07-01 00:00:00
abstract::Early estimates of the prevalence of TTV viremia in healthy adults of developed countries were in the order of 1--10 %, while similar estimates in Third World countries were considerably higher. Using three different PCRs, TTV-related DNA was detected in serum from 180 out of the 201 Norwegian blood donors tested, ind...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1062
更新日期:2001-07-01 00:00:00
abstract::Seasonal fluctuations in hepatitis A have been observed in the Netherlands related to Turkish and Moroccan children after visiting their home countries. This study determined the prevalence and associated factors of hepatitis A virus (HAV) antibodies in Turkish and Moroccan children in Rotterdam. A random sample was t...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10576
更新日期:2004-02-01 00:00:00
abstract::During 1999-2000, a sustained mumps outbreak in the highly vaccinated population in Singapore was attributed to vaccine failure associated with the Rubini vaccine strain. To explain this phenomenon, the complete nucleotide and amino acid sequences of the hemagglutinin-neuraminidase (HN) gene of eight mumps virus isola...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10393
更新日期:2003-06-01 00:00:00
abstract::The Markov model was introduced to simulate natural histories of hepatitis C virus (HCV) infection in men and women. The data set was constructed on 942 HCV carriers who were examined at least once a year without receiving antiviral therapies. Based on 2,251 patient-year data, the probabilities of transition between a...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10406
更新日期:2003-07-01 00:00:00
abstract::A previous study revealed a high prevalence of hepatitis B surface antigen (HBsAg) and hepatitis delta virus (HDV) RNA among 249 apparently healthy individuals (mean+/-standard deviation age, 48.4+/-13.9 years; 126 males and 123 females) in Ulaanbaatar, Mongolia. To investigate further the prevalence of HDV infection ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20363
更新日期:2005-07-01 00:00:00
abstract::It was reported previously that HCV can be transmitted from persistently infected human bone-marrow-derived B-lymphoblastoid cells (TO.FE(HCV)) to human hepatoma cells by cell-to-cell contact. The present study confirms and characterize further such type of HCV infection in vitro. TO.FE(HCV) cells were co-cultured wit...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20947
更新日期:2007-10-01 00:00:00