Abstract:
:The effects of 6-diazo-5-oxo-L-norleucine (DON), 2-deoxy-D-glucose (DOG), and tunicamycin (TM) on the replication of poliovirus (PV) and vesicular stomatitis virus (VSV) were examined. During a 48-hr replication period, TM, DON, and DOG inhibit VSV plaque formation in HEp-2 cells by 99.9%, 99.8%, and 99.9% respectively. Inhibition of VSV by DON is reversed with glutamine. Although all three agents are known to affect glycoprotein synthesis, DON and DOG also inhibit plaque formation of viruses devoid of structural glycoproteins. Thus, plaque formation of PV types 1 and 3 and Coxsackie B3 virus is delayed in HEp-2 and Buffalo green monkey kidney cells during exposure to these agents. Since these viruses do not contain glycoproteins and since concentrations up to 10 micrograms TM/ml cause no significant inhibition of PV, DON and DOG are affecting another viral or cellular process. Inhibition of PV replication by DON is reversed by addition of 25 mM glutamine or marginally by exposure to a combination of 5 mM concentrations of cytidine, uridine, adenosine monophosphate, and guanosine monophosphate. Inhibition of PV replication by DOG is reversed with 5 mM uridine alone. During DON exposure of HEp-2 cells infected with PV, the amount of 3H-uridine incorporation at 5.5 hr postinfection (pi) is reduced to 53% of untreated controls, an amount 11% greater than incorporation in cultures infected with PV but not treated with DON. These data indicate that the inhibition of PV replication by DON or DOG occurs at the level of viral RNA synthesis, while the primary target of these agents during VSV replication is probably glycosylation.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Goldstein G,Guskey LEdoi
10.1002/jmv.1890140210subject
Has Abstractpub_date
1984-01-01 00:00:00pages
159-67issue
2eissn
0146-6615issn
1096-9071journal_volume
14pub_type
杂志文章abstract::The prevalence of anti-HCV was studied in a South African area endemic for hepatitis B virus. A total of 35,685 volunteer blood donors (22,034 whites, 9,218 Asians, 3,077 Africans, 1,356 coloureds), 71 haemophiliacs, 84 chronic dialysis patients, 100 antenatal attenders, 212 nurses, and 20 HIV-positive male homosexual...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890400113
更新日期:1993-05-01 00:00:00
abstract:BACKGROUND:Recent influenza B/Victoria lineage viruses contain amino acid deletions at positions 162 to 164 of the haemagglutinin (HA) protein. These amino acid deletions have affected the detection of B/Victoria lineage viruses by the lineage-specific conventional reverse-transcription polymerase chain reaction (RT-PC...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25607
更新日期:2020-03-01 00:00:00
abstract::Cervical specimens collected from 163 cytologically healthy women were screened for the presence of human papillomavirus (HPV) DNA and anti-HPV secretory IgA antibodies. HPV DNA was detected by a general primer mediated polymerase chain reaction (PCR), which amplifies a conserved region from the L1 ORF of genital HPVs...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890430219
更新日期:1994-06-01 00:00:00
abstract::The λ-carrageenan (λ-car) is a potent and selective inhibitor of dengue virus (DENV) infection targeted to virus adsorption and internalization, due to the structural similarities with the mammalian cell receptor heparan sulfate. To further characterize the antiviral activity of λ-car, the selection and the phenotypic...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24457
更新日期:2016-07-01 00:00:00
abstract::To study the association between hepatitis B and schistosomiasis, 1,234 Egyptian males, ages 18 to 24, were interviewed, examined, and tested for Schistosoma mansoni infection and HBsAg. Sera from 91 (7.4%) of the study subjects were positive for HBsAg, and S. mansoni was found in the stools of 26.3%. There was no cor...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890230203
更新日期:1987-10-01 00:00:00
abstract::Patients attending the genitourinary medicine clinic at Watford General Hospital, UK, were examined for clinical signs of genital herpes infection. Genital swabs were taken from 194 patients (126 female, 68 male) who presented with genital ulceration or symptoms which were suggestive of genital herpes infection. Swabs...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:1998-06-01 00:00:00
abstract::Anti-influenza serum and nasal antibody titers were followed during a double-blind, placebo-controlled, randomized study assessing the prophylactic efficacy of 50, 100, and 200 mg/day of amantadine hydrochloride against experimental challenge with influenza A/Beth/1/85. The geometric mean titers (GMT) of serum hemaggl...
journal_title:Journal of medical virology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1002/jmv.1890270213
更新日期:1989-02-01 00:00:00
abstract::In patients infected with the hepatitis C virus (HCV), a heterogeneous population of viruses, so-called quasispecies exists in vivo. The hypervariable regions (HVR) within the second envelope gene (HCV-E2) show particularly highly intratypic variability and are considered to be the target of neutralizing antibodies. T...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(199711)53:3<245::aid-jmv11
更新日期:1997-11-01 00:00:00
abstract::Analysis of genetic variation in 16 varicella-zoster virus (VZV) isolates selected at random and circulating in the Irish population between March 2002 and February 2003 was carried out. A 919 bp fragment of the glycoprotein E gene (open reading frame 68) encompassing codon 150, at which a non-synonymous mutation defi...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20048
更新日期:2004-05-01 00:00:00
abstract::The mechanisms underlying chronicity of hepatitis C virus (HCV) infection are poorly understood, but the importance of impaired viral clearance by the immune system has been suggested. The prevalence of HCV infection of peripheral blood mononuclear cells (PBMC) was in investigated in 34 persistently infected patients ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890470112
更新日期:1995-09-01 00:00:00
abstract::Puumala and Tula viruses are hantaviruses found in Europe and are associated with the rodents Clethrionomys glareolus and Microtus arvalis, respectively. Puumala virus is associated with the human disease nephropathia epidemica. In Austria, ten clinically diagnosed cases of nephropathia epidemica, presumably caused by...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(199710)53:2<174::aid-jmv11
更新日期:1997-10-01 00:00:00
abstract::Comparison of RT-PCR assays established in house at various places revealed that laboratories could differ in sensitivity by as much as 1,000-fold in terms of the ability to detect measles virus sequences in clinical samples. The study indicates that PCR findings, positive or negative, are questionable if they are not...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10371
更新日期:2003-05-01 00:00:00
abstract::A recent cohort study1 at Suizhou in China identified that the proportion of subjects who wore eyeglasses was lower in hospitalized patients with coronavirus disease 2019 (COVID-19) than in the general population. This article is protected by copyright. All rights reserved. ...
journal_title:Journal of medical virology
pub_type: 信件
doi:10.1002/jmv.26845
更新日期:2021-02-02 00:00:00
abstract::The nucleotide sequences of the precore/core and X open reading frames (ORFs) of hepatitis B virus (HBV) were studied in four subjects who were serologically negative for anti-hepatitis B core antibody. These subjects were positive for serum hepatitis B surface antigen and were considered to be asymptomatic HBV carrie...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890460306
更新日期:1995-07-01 00:00:00
abstract::Hepatitis E virus (HEV), a positive-strand RNA agent, has been associated with enterically transmitted non-A, non-B hepatitis in Asia, Africa, and Mexico. To evaluate the role of HEV in an outbreak of hepatitis in Pakistan, we used immune electron microscopy to detect 1) antibody to HEV, for evidence of infection, and...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890360205
更新日期:1992-02-01 00:00:00
abstract::Chitin in the form of microparticles (chitin microparticles, CMP) has been demonstrated to be a potent stimulator of macrophages, promoting T-helper-1 (Th1) activation and cytokine response. In order to examine the mucosal adjuvant effect of CMP co-administered with influenza hemagglutinin (HA) vaccine against influen...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20247
更新日期:2005-01-01 00:00:00
abstract::Population-specific epidemiological data on human papillomavirus (HPV) infection are essential for formulating strategies to prevent cervical cancer. The age-specific prevalence of HPV infection was determined among 1,600 women enrolled for cervical screening in Macao. A U-shaped age-specific prevalence curve with a f...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21826
更新日期:2010-10-01 00:00:00
abstract::Adhesion molecules are essential for the immune response. They are involved in the regulation of cell-to-cell contact, thereby enabling leukocytes to communicate. Circulating forms of adhesion molecules are found in the serum of healthy individuals. Raised levels have been associated with disease severity in HCV and o...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:2001-09-01 00:00:00
abstract::Ideally, long-term lamivudine therapy should not induce tyrosine-methionine-aspartate-aspartate (YMDD) mutants (reverse transcription [rt]; rt M204I/V) in patients with chronic hepatitis B. There is little or no information on the clinical features of patients who do not develop such mutants. We analyzed 368 patients ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21681
更新日期:2010-04-01 00:00:00
abstract::A solid phase enzyme linked immunosorbent assay (ELISA) that detects IgM and IgG to hepatitis E virus (HEV) was used to study seroepidemiology in 40 healthy subjects and 227 consecutive patients with liver diseases in an endemic area. Fifty-two of the liver diseases patients (22.9 percent) had acute hepatitis E. In co...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890430316
更新日期:1994-07-01 00:00:00
abstract::Previous studies demonstrated that in vitro infection with influenza A viruses altered several functions of human monocytes-macrophages but did not detectably alter functions of human lymphocytes. However, both types of cells were infected, as determined by production and surface expression of viral antigens. In the c...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890270302
更新日期:1989-03-01 00:00:00
abstract::Overall, head and neck sqamous cell carcinoma accounts for more than 550,000 cases annually worldwide. It is well known that human papillomavirus (HPV) is the main risk factor for cervical cancer development. As the incidence and the mortality of cervical cancer are closely related to the HPV prevalence, we hypothesiz...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23750
更新日期:2014-03-01 00:00:00
abstract::Chronic hepatitis B patients with low pre-treatment HBeAg (and HBV-DNA) levels are more likely to respond to interferon-alpha therapy. In retrospect, this low level of HBeAg may have been reached just before the start of therapy. Pre-treatment changes in HBeAg levels were studied in 121 patients undergoing interferon-...
journal_title:Journal of medical virology
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:2001-03-01 00:00:00
abstract::Parechoviruses are emerging pathogens of humans often affecting the pediatric age group, with a growing line of evidence implicating them as agents of a broad spectrum of clinical syndromes in adults. However, because many clinicians are not familiar with the manifestation of the infections, they are not included in t...
journal_title:Journal of medical virology
pub_type: 杂志文章,评审
doi:10.1002/jmv.26395
更新日期:2020-08-06 00:00:00
abstract::Men who have sex with men (MSM) are an important risk group for human immunodeficiency virus (HIV) and at high-risk of herpesvirus infection. However, limited information on epidemiologic patterns of HHV8 and HSV2 among MSM is available in mainland China. A cross-sectional study of 486 participants was performed in Sh...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24718
更新日期:2017-05-01 00:00:00
abstract::Genotype II.4 noroviruses (NoVs) are a leading cause of epidemic acute gastroenteritis in children and adults worldwide. The prevalence of different NoV genotypes causing outbreaks and sporadic cases of acute gastroenteritis in the region of Valencia, Spain, during a 4-year period (2008-11) was investigated. NoVs were...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24112
更新日期:2015-04-01 00:00:00
abstract::Persistent Epstein-Barr virus (EBV) infection is controlled tightly by virus-specific T cells. EBV infection is reactivated intermittently over time, even in apparently healthy carriers. Changes in frequency and reactivity of memory T cells, particularly of CD8(+) origin, have not been assessed in this context. It is ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.22258
更新日期:2012-01-01 00:00:00
abstract::Sera from 31 human immunodeficiency virus (HIV)-infected patients, representing different clinical stages of HIV infection, were assayed for antibodies against measles and mumps viruses by various serological tests and compared to 23 healthy controls. Sera from four patients (two primary, one asymptomatic, and one acq...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890260107
更新日期:1988-09-01 00:00:00
abstract::A molecular epidemiological study on common diarrheal viruses was conducted in a children's hospital in Ho Chi Minh City between December 2005 and November 2006. Fecal samples were collected from 502 pediatric patients with acute gastroenteritis, and were screened for eight types of viral agents. Human astroviruses (H...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21032
更新日期:2008-02-01 00:00:00
abstract::The prevalence of HCV genotypes in four republics of the former Soviet Union (Russia, Belarus, Moldova, and Uzbekistan) was investigated. Overall, 197 HCV isolates from 66 blood donors and 131 patients with chronic hepatitis were typed. Viral sequences from sera of infected subjects were amplified by nested RT-PCR usi...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:1997-09-01 00:00:00