Abstract:
:Comparison of RT-PCR assays established in house at various places revealed that laboratories could differ in sensitivity by as much as 1,000-fold in terms of the ability to detect measles virus sequences in clinical samples. The study indicates that PCR findings, positive or negative, are questionable if they are not supported by the associated data demonstrating the overall sensitivity of the assay applied. Measles virus-specific RT-PCR-based assays need to be validated using standard virus preparation or nucleic acid-based target templates. A correlation between real-time quantitative PCR and the conventional PCR for measles virus is highly desirable.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Afzal MA,Osterhaus AD,Cosby SL,Jin L,Beeler J,Takeuchi K,Kawashima Hdoi
10.1002/jmv.10371keywords:
subject
Has Abstractpub_date
2003-05-01 00:00:00pages
171-6issue
1eissn
0146-6615issn
1096-9071journal_volume
70pub_type
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