Abstract:
:Oncogenic human papillomavirus (HPV), a causative agent of uterine cervical cancer, has also been detected in head and neck squamous cell cancers, especially in squamous cell carcinomas of the tonsils. However, the true HPV prevalence in normal and neoplasic oropharyngeal mucosa remains uncertain. To determine the prevalence of HPV DNA in normal oropharyngeal mucosa of cancer-free individuals, a study was carried out on 50 Brazilian subjects. PCR was performed to identify HPV DNA in samples from four sites in the oropharynx (tonsils, soft palate, base of the tongue, and back wall of the pharynx). For amplification of the HPV DNA, MY09/11 consensus primers were used, and specific genotypes were identified by dot-blot hybridization or cloning and sequencing. HPV DNA was present in 14.0% of the individuals, and the identified genotypes were 16, 18, 52, and 61. All these types are considered high-risk (HR) HPV. The tonsils and the soft palate were the sites with the highest HPV prevalence. This study shows the prevalence of HR HPV in the oropharynx of normal individuals. However, the prevalence of HPV is still unclear, and if HPV infection in a healthy it is not known individual predisposes to HPV-associated disease such as oropharyngeal cancer. Thus, it is important to assess the prevalence of HPV in cancer-free individuals, in order to compare it with the HPV prevalence in oropharyngeal carcinomas and to attempt to determine the true role of HPV in the development of head and neck squamous cell cancers.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
do Sacramento PR,Babeto E,Colombo J,Cabral Ruback MJ,Bonilha JL,Fernandes AM,Pereira Sobrinho JS,de Souza FP,Villa LL,Rahal Pdoi
10.1002/jmv.20583keywords:
subject
Has Abstractpub_date
2006-05-01 00:00:00pages
614-8issue
5eissn
0146-6615issn
1096-9071journal_volume
78pub_type
杂志文章abstract::Physicochemical studies of hepatitis B e antigen (HBeAg) revealed a clear cut difference between e1 and e2 antigen. The e1 antigen was found to have a MW of Ca 150,000 and a pI of 6.4-7.2, whereas both the MW and pI of the e2 antigen were heterogeneous depending upon the source of serum. Sera obtained from asymptomati...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890040105
更新日期:1979-01-01 00:00:00
abstract::Human T-lymphotropic virus type 1 (HTLV-1) infection causes adult T-cell leukemia and is characterized by long periods of clinical latency with low levels of viral production. Transcription of HTLV-1 is controlled through sequences in the promoter and enhancer regions of the long terminal repeat of the integrated prov...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:2000-10-01 00:00:00
abstract::The molecular epidemiology of hepatitis A virus (HAV) strains circulating in the St. Petersburg and Karelia regions was studied during 1997-2003. Hepatitis A virus RNA was isolated from both clinical samples (stools or sera) and environmental samples (sewage water). RT-PCR was carried out using different primer pairs ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20843
更新日期:2007-06-01 00:00:00
abstract::A highly sensitive one-step real-time RT-PCR method using a minor-groove-binding (MGB) probe was developed for detection and quantitation of severe febrile with thrombocytopenia syndrome virus (SFTSV). The assay could discriminate SFTSV infection from other related viral diseases in human with a minimum detection limi...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23415
更新日期:2013-02-01 00:00:00
abstract::Replication of HIV-1 inside host cells is dependent on both viral and host factors. MicroRNAs are small noncoding RNAs that regulate protein synthesis. MicroRNAs may control viral replication either by directly targeting the viral genome or indirectly through cellular proteins that are required during the viral lifecy...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26755
更新日期:2020-12-24 00:00:00
abstract::Rotavirus A (RVA) is the leading cause of acute viral gastroenteritis in children under 5 years of age worldwide. G9P[8] is a common RVA genotype that has been persistently prevalent in Jiangsu, China. To determine the genetic diversity of G9P[8] RVAs, 7 representative G9P[8] strains collected from Suzhou Children's H...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25214
更新日期:2018-09-01 00:00:00
abstract::Norovirus is one of the major causes of outbreaks and sporadic cases of acute gastroenteritis in young children worldwide. Obtaining local baseline information regarding this virus is important for developing and evaluating prevention strategies of norovirus transmission in children. The age, seasonal distribution and...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21569
更新日期:2009-10-01 00:00:00
abstract::Hepatitis C Virus (HCV) NS3 protease is an attractive target for antiviral agent development because it is required for viral replication. Because a stable cell culture system or small animal model to study HCV replication is not readily available, we constructed an in vitro model allowing the investigation of NS3 tra...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.2089
更新日期:2001-12-01 00:00:00
abstract::A 6-month-old girl with active human cytomegalovirus (HCMV) infection developed Reye's syndrome after vaccination. She suffered from uncommon complications of HCMV infection, including Coombs-negative hemolytic anemia and cardiac injury, but recovered after the appropriate treatment with prompt ganciclovir and symptom...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23475
更新日期:2013-03-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) circulating recombinant form (CRF) 07_BC strain has caused serious outbreaks among injection drug users in Taiwan since 2004. The objective of this study was to conduct a molecular epidemiological study of HCV genotypes in intravenous drug users in Taiwan. Blood samples and ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21658
更新日期:2010-01-01 00:00:00
abstract::The TT virus (TTV) was isolated recently from the serum of a patient with post-transfusion hepatitis. TTV infection is widespread in the general population, and its prevalence increases continuously with age. The pathogenic role of TTV in liver disease remains controversial, and the source of transmission is still unc...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20204
更新日期:2004-11-01 00:00:00
abstract::Previously, it was reported that productive viral infection, viral protein synthesis, and viral RNA replication of respiratory syncytial virus (RSV) operated efficiently in two human epithelial cell lines (HEp-2 and A549), but not in a human mast-cell line, HMC-1. Based on these observations, it was hypothesized that ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23196
更新日期:2012-02-01 00:00:00
abstract::To establish gut immunity in monkeys to polio-virus type 1, we fed four juvenile bonnet monkeys (Macaca radiata) with 100 median infectious doses of the Mahoney strain of virus. The duration of viral shedding in the throat and faeces and the titre of virus in faeces were measured. Eighteen days after the last monkey s...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890270208
更新日期:1989-02-01 00:00:00
abstract:AIM:To investigate the prevalence and characteristics of hepatitis B e antigen (HBeAg) negative/treatment naïve subjects with low hepatitis B virus (HBV) DNA levels (<10(4) copies/ml) and low alanine aminotransferase (ALT) levels (<2 × upper limit of normal) in patients with HBV-related hepatocellular carcinoma (HCC)....
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24217
更新日期:2015-10-01 00:00:00
abstract::Subgroup A respiratory syncytial viruses present in respiratory secretions and low passage level cell culture isolates were found to be markedly less susceptible to neutralization with monoclonal antibodies (MAbs) to the F glycoprotein than the cell culture adapted A2 virus strain. Low passage virus isolates collected...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20892
更新日期:2007-06-01 00:00:00
abstract::Human respiratory syncytial virus (HRSV) is major pathogen of lower respiratory tract infections in infants and young children worldwide. There have been many studies regarding HRSV subgroup A (HRSV-A) G protein genetic variability but little information about HRSV subtype B (HRSV-B) G protein genetic diversity and mo...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23960
更新日期:2015-01-01 00:00:00
abstract::Recent studies have identified epithelial cell populations in human livers that are similar to the "oval cells" and "transitional cells" seen in rat livers during the early stages of chemical carcinogenesis. It has been suggested that these cells might be precursors of hepatocytes and theoretically could be involved i...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890430304
更新日期:1994-07-01 00:00:00
abstract::This is an audit of a new technique to improve the colposcopy service. Samples were tested for high risk HPV DNA using Digene Hybrid Capture II. Sixty-four percent of the sampled women under 30 had detectable high risk HPV DNA, decreasing to 44% in 30--39 year olds and to 27% in women over 40. High risk HPV prevalence...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20401
更新日期:2005-08-01 00:00:00
abstract::Seoul virus (SEOV) is the etiologic agent of hemorrhagic fever with renal syndrome. It is carried by brown rats (Rattus norvegicus), a commensal rodent that closely cohabitates with humans in urban environments. SEOV has a worldwide distribution, and in Europe, it has been found in rats in UK, France, Sweden, and Belg...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25390
更新日期:2019-05-01 00:00:00
abstract::Recently, several clusters of hepatitis A have been observed among hemophiliacs linked to factor VIII concentrates treated for virus inactivation solely with the solvent/detergent (S/D) method, a procedure that does not affect nonenveloped viruses such as the hepatitis A virus (HAV). A new outbreak of hepatitis A in s...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(199902)57:2<91::aid-jmv1>3
更新日期:1999-02-01 00:00:00
abstract::Norovirus (NoV) is a major cause of nonbacterial acute gastroenteritis (AGE) outbreaks worldwide, with infections reported in semiclosed environments, particularly in hospitals and nursing homes. Astrovirus (HAstV) is prevalent worldwide, especially in developing countries. We aimed to determine the prevalence, spatia...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25395
更新日期:2019-06-01 00:00:00
abstract::A simple method is described for the precise quantification of infectious duck hepatitis B virus (DHBV) in cell culture, using a radioimmunofocus assay (RIFA). Primary duck hepatocyte cell cultures were infected with serial dilutions of viral samples as for a plaque assay, but then maintained with liquid overlay mediu...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:1997-08-01 00:00:00
abstract::Only recently have we begun to fully realize the diversity of virions within a single human isolate of the group B Coxsackieviruses. These intratypic (strain) differences may be one of the important factors influencing pathogenesis, e.g., myocarditis vs. pancreatitis. Yet, until the virion strains within a type can be...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890200103
更新日期:1986-09-01 00:00:00
abstract::Human T-cell lymphotropic virus 1 (HTLV-1) is associated with two progressive diseases: HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) and adult T-cell leukemia/lymphoma (ATLL). Although HTLV-1 proviral load (PVL) has been introduced as a risk factor for these diseases' progression, it is not suff...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24721
更新日期:2017-06-01 00:00:00
abstract::Recent studies have suggested a pathogenic role of human parvovirus B19 (B19) in the development of acute fulminant liver failure in children. The hypothesis was based on the detection of B19 DNA in 8 of 10 explanted livers of children requiring liver transplantation. In the present study, explanted livers from 43 adu...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.2047
更新日期:2001-10-01 00:00:00
abstract::Sapovirus, a member of the family Caliciviridae is one of the major causative agents of viral gastroenteritis affecting all age group. Sapovirus was detected in 25 of 917 stool specimens from infants and children with acute gastroenteritis in a Children Hospital in Dhaka City, Bangladesh during 2004-2005. All fecal sp...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20859
更新日期:2007-05-01 00:00:00
abstract::An attempt was made to identify factors influencing the cumulative probability of an increased alanine aminotransferase (ALT) level and hepatocarcinogenesis in hepatitis C patients with a normal ALT level initially. A total of 398 consecutive patients with a normal ALT level initially for 6 months or more and follow-u...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21342
更新日期:2009-03-01 00:00:00
abstract:BACKGROUND AND AIM:A meta-analysis was performed to identify COVID-19 patients presenting with gastrointestinal (GI) symptoms during the first and second pandemic waves and investigate their association with the disease outcomes. METHODS:A systematic search in PubMed, Scopus, Web of Science, ScienceDirect, and EMBASE ...
journal_title:Journal of medical virology
pub_type: 杂志文章,评审
doi:10.1002/jmv.26836
更新日期:2021-02-02 00:00:00
abstract::An enzyme-linked immunosorbent assay (ELISA) test using polyvalent antigens and antisera was used to detect Coxsackie B virus-specific IgM responses in 329 patients admitted to the Coronary Care Unit, Wellington Hospital, New Zealand over a 12-month period. The sera of 30 of 153 (19.6%) patients with acute myocardial ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890180211
更新日期:1986-02-01 00:00:00
abstract::The effects of 6-diazo-5-oxo-L-norleucine (DON), 2-deoxy-D-glucose (DOG), and tunicamycin (TM) on the replication of poliovirus (PV) and vesicular stomatitis virus (VSV) were examined. During a 48-hr replication period, TM, DON, and DOG inhibit VSV plaque formation in HEp-2 cells by 99.9%, 99.8%, and 99.9% respectivel...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890140210
更新日期:1984-01-01 00:00:00