Physicochemical heterogeneity of hepatitis B e antigen detected in asymptomatic carriers and carriers in a hemodialysis unit.

Abstract:

:Physicochemical studies of hepatitis B e antigen (HBeAg) revealed a clear cut difference between e1 and e2 antigen. The e1 antigen was found to have a MW of Ca 150,000 and a pI of 6.4-7.2, whereas both the MW and pI of the e2 antigen were heterogeneous depending upon the source of serum. Sera obtained from asymptomatic carriers were characterized by low titers of HBs antigen, HBc antigen and DNA polymerase and contained e2 antigen of larger molecular weight (200,000-300,000) with a narrow distribution range and a pI of 4.8 to 5.2 (type 1). On the other hand, the sera from patients in a hemodialysis unit who were HBs antigen carriers and had high titers of HBs antigen, HBc antigen and DNA polymerase contained e2 antigen of heterogeneous distribution in MW (from 300,000 to 70,000) and pI (type 2 and 3). The e2 antigen obtained from the higher MW type 3 serum had lower isoelectric points (pI 4.5 to 5.2) as was the case with e2 antigen obtained from asymptomatic carriers whereas relatively wide range of isoelectric points (pI 5.1 to 8.2) was found with the lower molecular weight e2 antigen.

journal_name

J Med Virol

authors

Yamada E,Ohori H,Ishida N

doi

10.1002/jmv.1890040105

subject

Has Abstract

pub_date

1979-01-01 00:00:00

pages

33-42

issue

1

eissn

0146-6615

issn

1096-9071

journal_volume

4

pub_type

杂志文章
  • Towards developing HIV-2 lentivirus-based retroviral vectors for gene therapy: dual gene expression in the context of HIV-2 LTR and Tat.

    abstract::Because of the distinct ability of retroviruses to integrate into the target cell genome and thus achieve long-term expression, retrovirus vectors hold great promise for stable gene transfer. Such vectors derived from human immunodeficiency retroviruses (HIVs) and other lentiviruses are envisioned to possess several a...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/(sici)1096-9071(199802)54:2<118::aid-jmv9>

    authors: Sadaie MR,Zamani M,Whang S,Sistron N,Arya SK

    更新日期:1998-02-01 00:00:00

  • Identification of a human group C rotavirus in Malaysia.

    abstract::Stool specimens from 334 infants and young children hospitalized with diarrhea in the General Hospital, Kuala Lumpur, Malaysia between August and November, 1987 were analyzed for the presence of rotavirus double-stranded (ds) RNA by polyacrylamide gel electrophoresis. Of the 334 specimens analyzed, 32 (9.6%) were posi...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890430302

    authors: Rasool NB,Hamzah M,Jegathesan M,Wong YH,Qian Y,Green KY

    更新日期:1994-07-01 00:00:00

  • Seroprevalence of hepatitis B virus infection in children in Taipei, 1989: five years after a mass hepatitis B vaccination program.

    abstract::A nationwide hepatitis B vaccination program was launched in Taiwan in 1984. To study the impact of this ongoing program on hepatitis B virus (HBV) infection, a follow-up seroepidemiologic study was carried out in 1989 in a Taipei district where pre-vaccination seroepidemiology had been studied. HBV markers were studi...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890340205

    authors: Tsen YJ,Chang MH,Hsu HY,Lee CY,Sung JL,Chen DS

    更新日期:1991-06-01 00:00:00

  • Anal HPV 16 and 18 viral load: A comparison between HIV-negative and -positive MSM and association with persistence.

    abstract::Does anal HPV viral load explain the difference in anal HPV persistence between HIV-negative and -positive men who have sex with men (MSM)? MSM ≥18 years were recruited in Amsterdam, the Netherlands, in 2010-2011. Anal self-swabs were collected every 6 months and genotyped (SPF10 -PCR-DEIA-LIPA25 -system). HPV16 and H...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.24898

    authors: Marra E,King A,van Logchem E,van der Weele P,Mooij SH,Heijman T,Meijer CJLM,Verhagen DWM,van der Sande MAB,Schim van der Loeff MF

    更新日期:2018-01-01 00:00:00

  • Immunohistological detection of human herpes virus 6 in formalin-fixed, paraffin-embedded lung tissues.

    abstract::Human herpes virus type 6 (HHV-6) infection is widespread in healthy individuals. The only definite disease association is with exanthem subitum in infants though the virus has been linked with a variety of other diseases including interstitial pneumonitis in bone marrow allograft recipients. In order to investigate t...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890410204

    authors: Pitalia AK,Liu-Yin JA,Freemont AJ,Morris DJ,Fitzmaurice RJ

    更新日期:1993-10-01 00:00:00

  • cDNA microarray analysis of HBV transgenic mouse liver identifies genes in lipid biosynthetic and growth control pathways affected by HBV.

    abstract::Hepatitis B virus (HBV) transgenic mice that replicate HBV in the liver generally do not exhibit gross liver pathology, while maintaining a high level (10(7) or greater) of viral titer in the blood. We have used this model to determine the minimum effects of HBV replication in the liver on cellular gene transcription,...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20427

    authors: Hajjou M,Norel R,Carver R,Marion P,Cullen J,Rogler LE,Rogler CE

    更新日期:2005-09-01 00:00:00

  • A rapid and efficient method BK polyomavirus genotyping by high-resolution melting analysis.

    abstract::A small percentage of renal patients become infected with the BK polyomavirus (BKV), a pathogenic virus that causes BKV-associated nephropathy (BKVN), after kidney transplantation. This study presents a simple, rapid, high-throughput method for BKV genotyping using high-resolution melt analysis (HRMA). Using this nove...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.22239

    authors: Matsuda Y,Qazi Y,Iwaki Y

    更新日期:2011-12-01 00:00:00

  • Association of cytomegalovirus infection with post-transplantation cardiac rejection as studied using the polymerase chain reaction.

    abstract::The relationship between cytomegalovirus (CMV) infection and cardiac allograft rejection is controversial, some authors reporting a significant association, others not, on the basis of the results of conventional virological diagnosis by culture or serology. This problem was reinvestigated in 88 patients using a semi-...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890420412

    authors: Fernando S,Booth J,Boriskin Y,Butcher P,Carrington D,Steel H,Tryhorn Y,Corbishley C,Keeling P,Murday A

    更新日期:1994-04-01 00:00:00

  • Persistence of high-risk human papillomavirus infection in a population-based cohort of Danish women.

    abstract::Persisting human papillomavirus (HPV) infection is a critical step in cervical carcinogenesis. This study was conducted to determine the type-specific HPV persistence and risk factors for persistence of high-risk HPV infections in a large cohort of Danish women. The study was based on a population-based prospective co...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21750

    authors: Nielsen A,Kjaer SK,Munk C,Osler M,Iftner T

    更新日期:2010-04-01 00:00:00

  • Attenuated Junin virus infection in Callithrix jacchus.

    abstract::Twenty marmosets, male Callithrix jacchus, were used during this study. Fifteen of the marmosets were inoculated with 5,000 TCID50 of the attenuated XJC13 strain of Junin virus by intramuscular route and five were left as uninoculated controls. Animals were observed for a 420-day period. In order to carry out virologi...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890150112

    authors: Avila MM,Frigerio MJ,Weber EL,Rondinone S,Samoilovich SR,Laguens RP,de Guerrero LB,Weissenbacher MC

    更新日期:1985-01-01 00:00:00

  • Comparison of a rapid culture method combining an immunoperoxidase test and a group specific anti-VP1 monoclonal antibody with conventional virus isolation techniques for routine detection of enteroviruses in stools.

    abstract::In order to shorten the time required for the detection of enteroviruses in stool specimens, an 18-h immunoperoxidase test combining low-speed centrifugation and the use of a group specific anti-VP1 monoclonal antibody (5-D8/1, Dako) was developed. This rapid culture assay (RCA) was compared blindly to a conventional ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/(sici)1096-9071(199803)54:3<204::aid-jmv11

    authors: Bourlet T,Gharbi J,Omar S,Aouni M,Pozzetto B

    更新日期:1998-03-01 00:00:00

  • Fitness of Japanese encephalitis virus to Neuro-2a cells is determined by interactions of the viral envelope protein with highly sulfated glycosaminoglycans on the cell surface.

    abstract::Genetically different subpopulations were identified and purified from Japanese Encephalitis virus (JEV). Those with small plaques (SPs; <2 mm in diameter), derived from strains of T1P1, CJN, and CC27, were more competent than those with large plaques (LPs; >5 mm in diameter) when passaged in Neuro-2a cells. Differenc...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20406

    authors: Chiou SS,Liu H,Chuang CK,Lin CC,Chen WJ

    更新日期:2005-08-01 00:00:00

  • Oyster-associated gastroenteritis in Australia: the detection of Norwalk virus and its antibody by immune electron microscopy and radioimmunoassay.

    abstract::Following widespread outbreaks of oyster-associated gastroenteritis in Australia during 1978 in which Norwalk virus was implicated as the causative agent, collaborative studies were undertaken between laboratories in Australia and the United States to confirm the etiology. Immune electron microscopy (IEM) techniques w...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890060103

    authors: Grohmann GS,Greenberg HB,Welch BM,Murphy AM

    更新日期:1980-01-01 00:00:00

  • Quantification of adenovirus species B and C viremia by real-time PCR in adults and children undergoing stem cell transplantation.

    abstract::Adenovirus infection during stem cell transplantation is associated with high morbidity and mortality. Adenovirus species B and C have been the main causes for these infections; however, epidemiological details about the species are still unclear. To clarify the contributions of species B and C adenovirus, the DNA was...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20796

    authors: Takayama R,Hatakeyama N,Suzuki N,Yamamoto M,Hayashi T,Ikeda Y,Ikeda H,Nagano H,Ishida T,Tsutsumi H

    更新日期:2007-03-01 00:00:00

  • Dominant replication of either virus in dual infection with hepatitis viruses B and C.

    abstract::To characterize the state of dual infection with hepatitis B virus (HBV) and hepatitis C virus (HCV), HBV-DNA and HCV-RNA levels were determined by spot hybridization or polymerase chain reaction in the sera of patients who were positive for both hepatitis B surface antigen and HCV antibody. Among 27 patients who show...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890450222

    authors: Koike K,Yasuda K,Yotsuyanagi H,Moriya K,Hino K,Kurokawa K,Iino S

    更新日期:1995-02-01 00:00:00

  • Prevalence of delta agent among Chinese in Taiwan and Los Angeles.

    abstract::The prevalence of delta agent among 120 Chinese patients with HBsAg-positive chronic active and hepatocellular carcinoma from Taiwan and Los Angeles were studied by serological assay for antibody to delta antigen and immunoperoxidase staining for delta antigen in the liver biopsies. Of 120 patients, only four with chr...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890140106

    authors: Govindarajan S,Lee SD,Tong MJ,Tsai YT,Lo KJ

    更新日期:1984-01-01 00:00:00

  • Case report: Eastern equine encephalitis virus imported to the UK.

    abstract::Eastern equine encephalitis (EEE) is rare, but the most severe of the mosquito-borne encephalitides in the United States with a high case fatality rate of 30%. Here, we present a patient with EEE. EEE virus causes sporadic human disease in the Eastern parts of the United States, but the case we describe was a Scottish...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21379

    authors: Harvala H,Bremner J,Kealey S,Weller B,McLellan S,Lloyd G,Staples E,Faggian F,Solomon T

    更新日期:2009-02-01 00:00:00

  • HEV and HAV seroprevalence in men that have sex with men (MSM): An update from Milan, Italy.

    abstract::Hepatitis E virus (HEV) is a feco-orally transmitted pathogen and one of the most common cause of acute hepatitis worldwide. Recent studies in developed countries suggested that a direct human-to-human contact such as for sexually transmitted diseases may play a significant role in the HEV spread. The aim of this stud...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.25052

    authors: Greco L,Uceda Renteria SC,Guarneri D,Orlandi A,Zoccoli A,Benardon S,Cusini M,Lunghi G

    更新日期:2018-08-01 00:00:00

  • Detection of human papillomavirus in esophageal carcinoma.

    abstract::The aim of the study was to assess the prevalence of human papillomavirus (HPV) in the esophagus in the coastal region of Eastern Guangdong, Southern China, an area with a high incidence of esophageal carcinoma. Fresh surgical resection esophageal specimens were obtained from 176 esophageal carcinoma patients admitted...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.10219

    authors: Shen ZY,Hu SP,Lu LC,Tang CZ,Kuang ZS,Zhong SP,Zeng Y

    更新日期:2002-11-01 00:00:00

  • Epidemiology of Varicella-Zoster Virus in England and Wales.

    abstract::Many countries are studying currently the possibility of mass vaccination against varicella. The objective of this study was to provide a complete picture of the pre-vaccine epidemiology of the Varicella-Zoster Virus in England and Wales to aid in the design of immunisation programs. Population-based data including ge...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.10313

    authors: Brisson M,Edmunds WJ

    更新日期:2003-01-01 00:00:00

  • Switching to tenofovir disoproxil fumarate vs continuing treatment in patients with chronic hepatitis B who maintain long-term virological response to entecavir therapy: A randomized trial.

    abstract::No controlled trial in patients with chronic hepatitis B virus (HBV) infection on long-term entecavir (ETV) treatment, comparing switching to tenofovir disoproxil fumarate (TDF) with continuing the therapy, has been reported. Twenty-seven nucleos(t)ide-naïve patients with chronic HBV who underwent ETV therapy for ≥5 y...

    journal_title:Journal of medical virology

    pub_type: 杂志文章,随机对照试验

    doi:10.1002/jmv.25442

    authors: Iida-Ueno A,Enomoto M,Kozuka R,Tamori A,Kawada N

    更新日期:2019-07-01 00:00:00

  • Case of Reye's syndrome accompanied by hemolytic anemia and cardiac injury after cytomegalovirus infection.

    abstract::A 6-month-old girl with active human cytomegalovirus (HCMV) infection developed Reye's syndrome after vaccination. She suffered from uncommon complications of HCMV infection, including Coombs-negative hemolytic anemia and cardiac injury, but recovered after the appropriate treatment with prompt ganciclovir and symptom...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.23475

    authors: Qi Y,Liu X,Xu W,Ruan Q

    更新日期:2013-03-01 00:00:00

  • UV-inactivated measles virus stimulates human and mice naive lymphocytes to proliferate in vitro.

    abstract::Ultra violet (UV) light-inactivated measles virus was used for the lymphocyte proliferation assay, where it caused consistently adult human peripheral blood monolymphocytes (PBML) proliferation which was not dependent on the measles virus complement fixation titre in the donor's sera. When the cord blood lymphocytes f...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890460209

    authors: Tu R

    更新日期:1995-06-01 00:00:00

  • HIV-1 variability and progression to AIDS: a longitudinal study.

    abstract::HIV-1 replicative activity and its relation to the clinical and immunological evolution of infection was studied in a group of 150 HIV-1 seropositive Italian i.v. drug abusers over a 1 year period. HIV-1 was isolated from 90 (60%) subjects; two groups of isolates were distinguished, according to replicative activity "...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890320411

    authors: Fiore JR,Calabró ML,Angarano G,De Rossi A,Fico C,Pastore G,Bianchi LC

    更新日期:1990-12-01 00:00:00

  • MIP-1 alpha and MIP-1 beta induction by dengue virus.

    abstract::Dengue virus (DV) infection can result in either a mild febrile illness known as dengue fever (DF) or a life-threatening disease called dengue hemorrhagic fever (DHF). DHF is more prevalent in patients undergoing secondary DV infection. This observation has led to the hypothesis that DHF may be the result of immune re...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.2037

    authors: Spain-Santana TA,Marglin S,Ennis FA,Rothman AL

    更新日期:2001-10-01 00:00:00

  • Characterization of natural Epstein-Barr virus infection and replication in smooth muscle cells from a leiomyosarcoma.

    abstract::Cells from a leiomyosarcoma tumor (LMS-1) from a patient with the acquired immunodeficiency syndrome (AIDS) were explanted, cultured in vitro, and studied by phase-contrast microscopy for morphologic and growth characteristics, immunostaining for cell markers, EBER in situ hybridization and polymerase chain reaction f...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:

    authors: Jenson HB,Montalvo EA,McClain KL,Ench Y,Heard P,Christy BA,Dewalt-Hagan PJ,Moyer MP

    更新日期:1999-01-01 00:00:00

  • Genome types of adenovirus type 7 isolated in Hiroshima City.

    abstract::One hundred thirty-two strains of adenovirus type 7 were isolated in Hiroshima City during the period 1995-1999. About 80% of the viruses were isolated from patients with respiratory illness, and about 70% were isolated from children younger than 6 years old. DNA restriction analysis was performed on 58 of the 132 iso...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.10287

    authors: Ikeda Y,Yamaoka K,Noda M,Ogino T

    更新日期:2003-02-01 00:00:00

  • Molecular epidemiology of rotavirus gastroenteritis in Japan during 2014-2015: Characterization of re-emerging G2P[4] after rotavirus vaccine introduction.

    abstract::Rotavirus vaccines have been available in Japan since 2011. This study conducted to monitor the trend of group A rotavirus (RVA) genotypes 3 years after vaccine introduction. A total of, 539 fecal samples were collected from children with acute gastroenteritis in six regions during July 2014-June 2015. Among them, 178...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.25067

    authors: Khandoker N,Thongprachum A,Takanashi S,Okitsu S,Nishimura S,Kikuta H,Yamamoto A,Sugita K,Baba T,Kobayashi M,Hayakawa S,Mizuguchi M,Ushijima H

    更新日期:2018-06-01 00:00:00

  • Protection against influenza virus infection by intranasal administration of hemagglutinin vaccine with chitin microparticles as an adjuvant.

    abstract::Chitin in the form of microparticles (chitin microparticles, CMP) has been demonstrated to be a potent stimulator of macrophages, promoting T-helper-1 (Th1) activation and cytokine response. In order to examine the mucosal adjuvant effect of CMP co-administered with influenza hemagglutinin (HA) vaccine against influen...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20247

    authors: Hasegawa H,Ichinohe T,Strong P,Watanabe I,Ito S,Tamura S,Takahashi H,Sawa H,Chiba J,Kurata T,Sata T

    更新日期:2005-01-01 00:00:00

  • Diagnosis of COVID-19 pneumonia despite missing detection of viral nucleic acid and initially inconspicuous radiologic findings.

    abstract::The diagnosis of coronavirus disease 2019 (COVID-19) is mainly based on a positive severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) polymerase chain reaction (PCR) result. PCR samples are obtained from upper or lower respiratory tract specimens. However, the sensitivity of PCR is known to have some limitat...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.26153

    authors: Schiller M,Wydra S,Kerl HU,Kick W

    更新日期:2020-11-01 00:00:00