Abstract:
:It has been generally believed that the Epstein-Barr virus (EBV)-encoded small RNA 1 and 2 (EBER1 and EBER2) genes are conserved as two families that correlated with type 1 (B95-8) and type 2 (AG876 or P3HR-1) EBV strains. EBER polymorphism and its association with EBV-associated disease have not received much attention. To explore the variations of EBER genes in different malignancies and healthy donors, the sequences of EBER genes were analyzed in 154 EBV-positive samples, including 47 nasopharyngeal carcinoma (NPC), 50 EBV-associated gastric carcinoma (EBVaGC) biopsies and 57 throat washing (TW) samples from healthy donors in northern China, where NPC is non-endemic. Three main distinct variants of EBER genes, designated as EB-6m, EB-8m, and EB-10m, were identified. EB-6m had a previously identified EBER sequence identical to P3HR-1 and was found in 33/47 (70.2%) NPC, 48/50 (96.0%) EBVaGC, and 54/57 (94.7%) TW isolates. EB-8m and EB-10m were new EBER variants with more mutations in EBER2 genes. They were found in 13/47 (27.7%) NPC cases, whereas in only 1/50 (2.0%) EBVaGC cases and not found in TW cases. The distributions were significantly different (P < 0.05). Other five isolates (one NPC, one EBVaGC and three TW cases) showed different sequences and could not be assigned to any of the three groups. Type 1 EBV strains showed heterogeneous in terms of EBER variants. These results suggest that EBER locus can be useful to identify different EBV isolates, and it would be interesting to evaluate the association of EBER polymorphisms with EBV-associated tumors.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Wang Y,Zhang X,Chao Y,Jia Y,Xing X,Luo Bdoi
10.1002/jmv.21714subject
Has Abstractpub_date
2010-05-01 00:00:00pages
829-36issue
5eissn
0146-6615issn
1096-9071journal_volume
82pub_type
杂志文章abstract::Mixed cryoglobulinemia is a lymphoproliferative disorder associated with hepatitis C virus (HCV). In patients chronically affected by HCV the prevalence of mixed cryoglobulinemia is variable ranging from 0% to 56%. To verify whether polyomaviruses (PyV) play a role in this disorder a total of 222 blood samples from 63...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23867
更新日期:2014-04-01 00:00:00
abstract::This prospective study aimed to determine factors associated with detection of very low-level viremia in patients infected with HIV-1 with virological success following HAART introduction. Fifty-seven patients, mostly (n = 51, 89%) treated with a protease inhibitor-based regimen, were included and followed for 2 years...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23553
更新日期:2013-06-01 00:00:00
abstract::In mid-December 2019, a disease caused by infection with severe acute respiratory syndrome coronavirus-2, which began in Wuhan, China, has spread throughout the country and many countries around the world. The number of children with coronavirus disease-2019 (COVID-19) has also increased significantly. Although inform...
journal_title:Journal of medical virology
pub_type: 杂志文章,评审
doi:10.1002/jmv.25807
更新日期:2020-07-01 00:00:00
abstract::It has been reported that low-plasma cytomegalovirus (CMV) DNA loads are associated with an increased risk of overall mortality in allogeneic hematopoietic stem cell transplantation (allo-HSCT). Utilizing a conservative strategy for initiation of preemptive antiviral therapy (>1500 IU/mL), we characterized the virolog...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25426
更新日期:2019-06-01 00:00:00
abstract::One hundred twenty strains of adenovirus types 1, 2, 5, and 6 (subgenus C), isolated at various times and places, were tested by neutralization and hemagglutination inhibition with two antisera of each type. Except for one type 5 strain they reacted largely type-specifically in both tests and neither qualitative nor q...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890180310
更新日期:1986-03-01 00:00:00
abstract::A common receptor for coxsackie B virus and adenovirus has been described recently in cells of human and murine origin. Since the established cell line A-549 is suitable for adenoviruses, the potential use of A-549 cells for the isolation of coxsackie B viruses from clinical samples was investigated. All throat swabs ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:2001-11-01 00:00:00
abstract::Knowledge of the precise timing of SARS-CoV-2 infection may be of clinical and epidemiological relevance. The presence of low-avidity IgGs has conventionally been considered an indicator of recent infection. Here, we carried out qualitative assessment of SARS-CoV-2-specific antibody avidity using an urea (6M) dissocia...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26344
更新日期:2021-02-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) circulating recombinant form (CRF) 07_BC strain has caused serious outbreaks among injection drug users in Taiwan since 2004. The objective of this study was to conduct a molecular epidemiological study of HCV genotypes in intravenous drug users in Taiwan. Blood samples and ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21658
更新日期:2010-01-01 00:00:00
abstract::A pathogenic role of precore-defective mutation in the onset of fulminant hepatitis B has been suggested. However, precore-defective mutants do not always cause fulminant hepatitis B and are not always isolated from affected patients. These findings strongly suggest the presence of some additional important mutations ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890470304
更新日期:1995-11-01 00:00:00
abstract::A modified centrifugation culture technique and a polymerase chain reaction (PCR) is described for detection of early antigen and IE antigen DNA, respectively, for rapid and sensitive monitoring of active cytomegalovirus (HCMV) infection after organ transplantation. In a preliminary study, 541 clinical specimens (bloo...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890380404
更新日期:1992-12-01 00:00:00
abstract::The complete VP1 protein of EV71 was truncated into six segments and fused to the C-terminal ends of full-length nucleocapsid protein (NPfl) and truncated NP (NPt; lacks 20% amino acid residues from its C-terminal end) of newcastle disease virus (NDV). Western blot analysis using anti-VP1 rabbit serum showed that the ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20668
更新日期:2006-08-01 00:00:00
abstract::Exacerbations of chronic hepatitis B have been associated with accumulation of mutations in the HBV core gene, with amino acid (aa) substitutions clustering between aa 50 and 69. This region of the nucleocapsid protein is known as an immunodominant epitope for CD4+ T-lymphocytes, however the impact of these mutations ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20016
更新日期:2004-03-01 00:00:00
abstract::The aim of this study was to compare the data obtained using the new LIAISON® XL chemiluminescence system to search for HBsAg, anti-HCV, and anti-HIV1-2/p24 Ag with those obtained using the VITROS system currently adopted by the Microbiology Unit of the Hospital of Legnano. Routine samples of patients who were referre...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24648
更新日期:2017-03-01 00:00:00
abstract::Attachment of hepatitis B virus to a hepatoblastoma cell line (HepG2) was examined using a synthetic peptide corresponding to the pre-S1 (21-47) region of the envelope protein. Scatchard analysis revealed a single class binding site of Kd 104 +/- 27 nM/l and 5.4 +/- 1.2 x 10(5) sites per cell. Competition of HBV with ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890370208
更新日期:1992-06-01 00:00:00
abstract::There are very few studies in search of an alternate and convenient diagnostic tool which can substitute nasopharyngeal swab (NPS) specimen for detection of SARS-CoV-2. In the study we analyzed, the comparison and agreement between the feasibility of using the saliva in comparison to NPS for diagnosis of SARS-CoV-2. A...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26719
更新日期:2020-12-09 00:00:00
abstract::Individual specific antigenic rubella virus (RV) structural proteins are required for accurate serological diagnosis of acute and congenital rubella infections as well as rubella immune status. The RV envelope glycoprotein E1 is the major target antigen and plays an important role in viral-specific immune responses. T...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890440214
更新日期:1994-10-01 00:00:00
abstract::Hepatitis C virus (HCV) variation in specific T-cell epitopes may represent a mechanism of viral persistence in chronic infection. We examined the HCV non-structural protein 3 (NS3), including the immunologically relevant epitopes HCV NS3-2 KLVALGINAV (human leukocyte antigen [HLA]-A2-restricted) and HCV NS3-1391 LIFC...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20036
更新日期:2004-04-01 00:00:00
abstract::Monoclonal antibodies were used for detecting varicella-zoster virus (VZV) by immunofluorescence (IF) in clinical specimens inoculated on shell vial cultures. Vesicles of 74 patients with varicella or herpes zoster-like eruptions were tested by this method and by conventional cell culture. Further diagnostic tests wer...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890280102
更新日期:1989-05-01 00:00:00
abstract::Rapid diagnosis of human herpesvirus primary infections or reactivations is facilitated by quantitative PCRs. Quantitative PCR assays with a standard thermal cycling profile permitting simultaneous detection of herpes simplex virus (HSV), varicella zoster virus (VZV), cytomegalovirus (CMV), Epstein-Barr virus (EBV), a...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21095
更新日期:2008-03-01 00:00:00
abstract::Sera from patients with sandfly fever caused by Toscana virus (TOSV) infection were tested by immunoblot for specific antibody response to TOSV derived from infected Vero-E6 cells. The 28 kDa TOSV nucleoprotein (N) was identified as the major immunodominant protein recognized by immunoblot. In sera of patients with ac...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(SICI)1096-9071(199606)49:2<83::AID-JMV2>3
更新日期:1996-06-01 00:00:00
abstract::Varicella in the immunocompetent adult is an infrequent but potentially serious infection. Previous studies in immunocompetent hosts and normal adults have demonstrated the value of intravenous acyclovir in the treatment of varicella-zoster virus infections. Oral acyclovir has also shown efficacy in both normal adults...
journal_title:Journal of medical virology
pub_type: 杂志文章,评审
doi:10.1002/jmv.1890410517
更新日期:1993-01-01 00:00:00
abstract::Throughout most of the last decade, B/Yamagata/16/88-lineage influenza viruses were predominant among the B isolates circulating worldwide, whereas B/Victoria/2/87-lineage viruses were isolated infrequently and restricted geographically to eastern Asia. During the 2001-02 influenza season, B/Victoria/2/87-lineage viru...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20225
更新日期:2004-12-01 00:00:00
abstract::A reverse transcription-polymerase chain reaction (RT-PCR) was developed for the detection of Chikungunya virus infection. Based on the nonstructural protein 1 (nsP1) and glycoprotein E1 (E1) genes of Chikungunya, two primer sets were designed. Total RNA were extracted from the cell culture fluid of Aedes albopictus C...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10085
更新日期:2002-07-01 00:00:00
abstract::The immunogenicity of the Russian cold-adapted (ca) donor stains, A/Leningrad (Len)/134/17/57 and A/Leningrad/134/47/57, and the US strain A/Ann Arbor (AA)/6/60-ca, were compared in BALB/c mice with their respective wild-type parental viruses. Each ca donor strain was less immunogenic than its wild-type parent. The va...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:2001-09-01 00:00:00
abstract::Analysis of the HIV-1 V3 quasispecies present in an individual at the time of seroconversion was carried out. The polymerase chain reaction (PCR) was used to amplify proviral HIV-1 DNA extracted from peripheral blood mononuclear cells from a patient who was viraemic (p24 = 15 pg/ml) and had an equivocal HIV-1 antibody...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890410403
更新日期:1993-12-01 00:00:00
abstract:BACKGROUND:The data on the seasonality of respiratory viruses helps to ensure the optimal vaccination period and to monitor the possible outbreaks of variant type. OBJECTIVES:This study was designed to describe the molecular epidemiology and seasonality of acute respiratory infection (ARI)-related respiratory viruses ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25464
更新日期:2019-08-01 00:00:00
abstract::Paired sera, saliva and cervicovaginal secretions from 17 HTLV-I-infected women (19-75 yr) were tested for total IgA and IgG, for IgA and IgG to the immunodominant region gp46/175-Pro-199, for serum IgG to the neutralizing domains gp46/ 190-Pro-199 and gp46/190-Ser-199, or for tax-rex proviral HTLV-DNA. Serum antibodi...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(SICI)1096-9071(199609)50:1<42::AID-JMV9>3
更新日期:1996-09-01 00:00:00
abstract::West Nile virus (WNV) activity has fluctuated in the south-central United States since its introduction. Seasonal outbreaks are common, with three in Oklahoma during 2003, 2007, and 2012. Morbidity and mortality rates vary during each outbreak. Long-term neurologic sequelae in association with West Nile virus disease ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24235
更新日期:2015-10-01 00:00:00
abstract::Lack of recognition of human immunodeficiency virus (HIV) infection especially in children and delayed implementation of effective control programs makes HIV infection as a major cause for concern. Information on HIV epidemiology in Iran as well as other Islamic countries is limited. The aim of our study was to descri...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24294
更新日期:2016-01-01 00:00:00
abstract::Enteroviruses are responsible for hand, foot, and mouth disease, and have caused many deaths in China during recent years. But the natural history of enterovirus infection in children, especially asymptomatic children, is not yet clear. From April 2011 to May 2012, 505 stool and throat swab samples of children attendi...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23884
更新日期:2014-09-01 00:00:00