Abstract:
:Sera from patients with sandfly fever caused by Toscana virus (TOSV) infection were tested by immunoblot for specific antibody response to TOSV derived from infected Vero-E6 cells. The 28 kDa TOSV nucleoprotein (N) was identified as the major immunodominant protein recognized by immunoblot. In sera of patients with acute TOSV infection, specific antibodies of the IgM, IgA, and IgG class were detected. Using sandfly fever virus, serotypes Sicilian (SFSV) and Naples (SFNV), as antigens for immunoblot, TOSV antibody-positive sera cross-reacted with the corresponding N proteins. These sera reacted for IgM and IgG by SFSV immunoblot, and for IgM by SFNV immunoblot. The diagnosis of sandfly fever may be confirmed by TOSV immunoblot.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Schwarz TF,Gilch S,Pauli C,Jäger Gdoi
10.1002/(SICI)1096-9071(199606)49:2<83::AID-JMV2>3subject
Has Abstractpub_date
1996-06-01 00:00:00pages
83-6issue
2eissn
0146-6615issn
1096-9071pii
10.1002/(SICI)1096-9071(199606)49:2<83::AID-JMV2>3journal_volume
49pub_type
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