Expression and characterization of a soluble rubella virus E1 envelope protein.

Abstract:

:Individual specific antigenic rubella virus (RV) structural proteins are required for accurate serological diagnosis of acute and congenital rubella infections as well as rubella immune status. The RV envelope glycoprotein E1 is the major target antigen and plays an important role in viral-specific immune responses. The native virion is difficult to produce in large quantities and the protein subunits are also difficult to isolate without loss of antigenicity. The production of a soluble RV E1 (designated E1 delta Tm) using the baculovirus-insect cell expression system is described. In contrast to wild-type RV E1, the genetically engineered E1 delta Tm protein lacks a transmembrane anchor. It behaved as a secretory protein and was secreted abundantly from insect cells. Pulse-chase studies were used to examine the synthesis, glycosylation, and secretion of E1 delta Tm by the insect cells. The secreted E1 delta Tm protein was purified from serum-free medium by one-step immunochromatography. The purified E1 delta Tm protein retained full antigenicity and may be a convenient source of E1 protein for use in diagnostic assay and rubella vaccine development.

journal_name

J Med Virol

authors

Seto NO,Gillam S

doi

10.1002/jmv.1890440214

subject

Has Abstract

pub_date

1994-10-01 00:00:00

pages

192-9

issue

2

eissn

0146-6615

issn

1096-9071

journal_volume

44

pub_type

杂志文章
  • Rapid development of genotypic resistance to lamivudine when combined with zidovudine in pregnancy.

    abstract::The prevention of mother to child transmission of HIV-1 by zidovudine monotherapy is well known, but increasingly combination anti-retroviral therapy is prescribed during pregnancy. In this prospective study, 19 pregnant women with human immunodeficiency virus-1 (HIV-1) infection who elected to take anti-retroviral th...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/(sici)1096-9071(199911)59:3<364::aid-jmv17

    authors: Clarke JR,Braganza R,Mirza A,Stainsby C,Ait-Khaled M,Wright A,Lyall H,Parker D,McClure MO,Weber JN,Taylor GP

    更新日期:1999-11-01 00:00:00

  • Prediction of the virological response to etravirine in clinical practice: Comparison of three genotype algorithms.

    abstract::The current Agence Nationale de Recherche sur le SIDA (ANRS)/International AIDS Society (IAS) algorithm predicts resistance to etravirine for viruses harboring >/=3 mutations from a list of 13 reverse transcriptase (RT) mutations. Two weighted algorithms, best correlated with fold changes to etravirine, have been desc...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21461

    authors: Cotte L,Trabaud MA,Tardy JC,Brochier C,Gilibert RP,Miailhes P,Trépo C,André P

    更新日期:2009-04-01 00:00:00

  • Molecular identification of a novel human rotavirus in relation to subgroup and electropherotype of genomic RNA.

    abstract::A total of 41 stool rotavirus specimens collected from children with acute diarrhea at four different locations in Akita Prefecture, Japan, during the peak of the winter diarrhea epidemic in 1988 were analyzed by polyacrylamide gel electrophoresis of viral RNA in conjunction with subgrouping assay. We found that a sin...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890280311

    authors: Nakagomi O,Oyamada H,Kuroki S,Kobayashi Y,Ohshima A,Nakagomi T

    更新日期:1989-07-01 00:00:00

  • Colposcopy, punch biopsy, in situ DNA hybridization, and the polymerase chain reaction in searching for genital human papillomavirus (HPV) infections in women with normal PAP smears.

    abstract::To assess the prevalence of HPV infection in the genital tracts of women with normal PAP smears, a random series of 109 women was reexamined using colposcopy, a further PAP smear, and punch biopsies taken from the cervix (in 33 cases), vagina (212 cases), and anus (20 cases). The biopsy material was examined using rou...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890310404

    authors: Syrjänen S,Saastamoinen J,Chang FJ,Ji HX,Syrjänen K

    更新日期:1990-08-01 00:00:00

  • Methylation of Epstein-Barr virus Rta promoter in EBV primary infection, reactivation and lymphoproliferation.

    abstract::During Epstein-Barr virus (EBV) latency, the EBV genome is largely silenced by methylation. This silencing is overturned during the switch to the lytic cycle. A key event is the production of the viral protein Zta which binds to three Zta-response elements (ZRE) from the Rta promoter (Rp), two of which (ZRE2 and ZRE3)...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.24524

    authors: Germi R,Guigue N,Lupo J,Semenova T,Grossi L,Vermeulen O,Epaulard O,de Fraipont F,Morand P

    更新日期:2016-10-01 00:00:00

  • Naturally occurring hepatitis B virus X deletions and insertions among Korean chronic patients.

    abstract::Deletions and insertions in the hepatitis B virus (HBV) X region have been associated with severe forms of liver disease, including hepatocellular carcinoma (HCC). However, the molecular epidemiologic features of this virus have been described rarely. Deletions and insertions in the X region were determined by direct ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21938

    authors: Lee SA,Mun HS,Kim H,Lee HK,Kim BJ,Hwang ES,Kook YH,Kim BJ

    更新日期:2011-01-01 00:00:00

  • A new cluster of hepatitis A infection in hemophiliacs traced to a contaminated plasma pool.

    abstract::Recently, several clusters of hepatitis A have been observed among hemophiliacs linked to factor VIII concentrates treated for virus inactivation solely with the solvent/detergent (S/D) method, a procedure that does not affect nonenveloped viruses such as the hepatitis A virus (HAV). A new outbreak of hepatitis A in s...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/(sici)1096-9071(199902)57:2<91::aid-jmv1>3

    authors: Chudy M,Budek I,Keller-Stanislawski B,McCaustland KA,Neidhold S,Robertson BH,Nübling CM,Seitz R,Löwer J

    更新日期:1999-02-01 00:00:00

  • Genetic analysis of HAV strains isolated from patients with acute hepatitis in Korea, 2005-2006.

    abstract::Hepatitis A virus (HAV) is a causative agent of acute viral hepatitis, which represents a significant public health problem. HAV is usually transmitted by oral-fecal route and prevalent not only in developing countries but also in developed countries worldwide. To characterize the HAV wild type strains circulating in ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21127

    authors: Yun H,Kim S,Lee H,Byun KS,Kwon SY,Yim HJ,Lim YS,Jeong SH,Jee Y

    更新日期:2008-05-01 00:00:00

  • Repeated HIV-1 resistance genotyping external quality assessments improve virology laboratory performance.

    abstract::The performance of French virology laboratories belonging to the ANRS network has been assessed annually for 3 years. The performance of these laboratories was compared between the years 2002 and 2003. Ten and 7 coded samples were sent to 38 virology laboratories in 2002 and 45 virology laboratories in 2003, respectiv...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20522

    authors: Descamps D,Delaugerre C,Masquelier B,Ruffault A,Marcelin AG,Izopet J,Chaix ML,Calvez V,Brun-Vézinet F,Costagliola D

    更新日期:2006-02-01 00:00:00

  • Serological and molecular prevalence of hepatitis E virus among blood donors from Uruguay.

    abstract::Hepatitis E virus (HEV) infection is considered a neglected disease of major concern in developed countries. Clinically, HEV occurs as an acute and self-limited disease, though chronic cases mostly associated to HEV-3 are now being commonly reported in immunocompromised individuals and solid organ transplant recipient...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.26231

    authors: Bangueses F,Abin-Carriquiry JA,Cancela F,Curbelo J,Mirazo S

    更新日期:2020-06-27 00:00:00

  • Experimental evidence of hepatitis A virus infection in pigs.

    abstract::Hepatitis A virus (HAV) is the leading cause of acute viral hepatitis worldwide, with HAV infection being restricted to humans and nonhuman primates. In this study, HAV infection status was serologically determined in domestic pigs and experimental infections of HAV were attempted to verify HAV infectivity in pigs. An...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.24386

    authors: Song YJ,Park WJ,Park BJ,Kwak SW,Kim YH,Lee JB,Park SY,Song CS,Lee SW,Seo KH,Kang YS,Park CK,Song JY,Choi IS

    更新日期:2016-04-01 00:00:00

  • A randomised controlled trial of recombinant interferon-gamma in Chinese patients with chronic hepatitis B virus infection.

    abstract::Nineteen Chinese patients with chronic hepatitis B virus (HBV) infection, seropositive for HBV e antigen (HBeAg) and HBV DNA on at least three occasions in 6 months, were randomised to receive either recombinant human interferon-gamma (rIFN gamma) 0.1 mg/m2 intramuscularly thrice weekly for 16 weeks (n = 11) or no ant...

    journal_title:Journal of medical virology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1002/jmv.1890340310

    authors: Lau JY,Lai CL,Wu PC,Chung HT,Lok AS,Lin HJ

    更新日期:1991-07-01 00:00:00

  • Seroepidemiology of hepatitis A, B, C, and E viruses infection among preschool children in Taiwan.

    abstract::Taiwan was a hyperendemic area for hepatitis A and B viruses (HAV and HBV) infection before late 1980s. To study the seroprevalence of hepatitis A, B, C, and E viruses (HCV and HEV) infection among preschool children in Taiwan, a community-based survey was carried out in 54 kindergartens in 10 urban areas, 10 rural ar...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20517

    authors: Lin JB,Lin DB,Chen SC,Chen PS,Chen WK

    更新日期:2006-01-01 00:00:00

  • Clinico-pathological significance of hepatitis C virus core antigen levels in chronic infection.

    abstract::Hepatitis C virus core antigen (HCVcoreAg) may be measured in serum with a sensitive, recently validated assay. Beyond its value as a marker of viral infection, there are little data on its relation with clinical, histological, and virological parameters. In this study, the significance of HCVcoreAg levels was studied...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.23672

    authors: Durante-Mangoni E,Vallefuoco L,Sorrentino R,Iossa D,Perna E,Molaro R,Braschi U,Zampino R,Sodano G,Adinolfi LE,Utili R,Portella G

    更新日期:2013-11-01 00:00:00

  • Human hepatitis B virus X protein augments the DNA binding of nuclear factor for IL-6 through its basic-leucine zipper domain.

    abstract::The X gene product of human hepatitis B virus, HBx, transactivates the expression of viral and cellular genes through a wide variety of cis elements, including the nuclear factor for IL-6 (NF-IL6) binding sites, although HBx does not appear to bind DNA directly. We previously reported that HBx transactivated the inter...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/(sici)1096-9071(199905)58:1<11::aid-jmv2>3

    authors: Ohno H,Kaneko S,Lin Y,Kobayashi K,Murakami S

    更新日期:1999-05-01 00:00:00

  • Occurrence rate and genotype distribution of the JC virus (JCV) in a sample from the Polish population.

    abstract::The JC virus (JCV) is a common human virus persisting in renal tissue. In immunocompromised individuals it may reactivate and cause progressive multifocal leukoencephalopathy (PML). JCV has also been implicated in cancerogenesis, leading to various brain tumors and cancers of gastrointestinal tract. In this study the ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21153

    authors: Kmieciak D,Debicki S,Trzeciak WH

    更新日期:2008-06-01 00:00:00

  • Detection by PCR of human polyomaviruses BK and JC in immunocompromised individuals and partial sequencing of control regions.

    abstract::Immunocompromised individuals were tested for the presence of the human polyomaviruses JC (JCV) and BK (BKV) by the polymerase chain reaction (PCR). The use of appropriate primers in a nested PCR allowed the detection of both viruses simultaneously. Viruses were differentiated by restriction fragment length analysis o...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890420208

    authors: Schätzl HM,Sieger E,Jäger G,Nitschko H,Bader L,Ruckdeschel G,Jäger G

    更新日期:1994-02-01 00:00:00

  • Surveillance of measles in the Sudan using filter paper blood samples.

    abstract::Dried blood spots collected on filter paper are considered potential clinical specimens for measles surveillance because of their ease of collection, storage, and transport. The usefulness of these samples for surveillance of measles was evaluated in a field setting. Blood spots were collected by finger-prick from 316...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20136

    authors: El Mubarak HS,Yüksel S,Mustafa OM,Ibrahim SA,Osterhaus AD,de Swart RL

    更新日期:2004-08-01 00:00:00

  • Hemagglutinin-neuraminidase sequence and phylogenetic analyses of mumps virus isolates from a vaccinated population in Singapore.

    abstract::During 1999-2000, a sustained mumps outbreak in the highly vaccinated population in Singapore was attributed to vaccine failure associated with the Rubini vaccine strain. To explain this phenomenon, the complete nucleotide and amino acid sequences of the hemagglutinin-neuraminidase (HN) gene of eight mumps virus isola...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.10393

    authors: Lim CS,Chan KP,Goh KT,Chow VT

    更新日期:2003-06-01 00:00:00

  • Floating density of hepatitis C virus particles and response to interferon treatment.

    abstract::Hepatitis C virus (HCV) particles can be classified into two major fractions according to their floating density in serum. However, the genomic heterogeneity of each fraction and the relationship between this viral characteristic and interferon (IFN) response in patients with chronic hepatitis are not known. In this s...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:

    authors: Sakai A,Kaneko S,Matsushita E,Kobayashi K

    更新日期:1998-05-01 00:00:00

  • Seroprevalence rates of HPyV6, HPyV7, TSPyV, HPyV9, MWPyV and KIPyV polyomaviruses among the healthy blood donors.

    abstract::Human polyomaviruses HPyV6, HPyV7, TSPyV, HPyV9, MWPyV, and KIPyV have been discovered between 2007 and 2012. TSPyV causes a rare skin disease trichodysplasia spinulosa in immunocompromised patients, the role of remaining polyomaviruses in human pathology is not clear. In this study, we assessed the occurrence of seru...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.24440

    authors: Šroller V,Hamšíková E,Ludvíková V,Musil J,Němečková Š,Saláková M

    更新日期:2016-07-01 00:00:00

  • Development of cell-mediated cytotoxic immunity to respiratory syncytial virus in human infants following naturally acquired infection.

    abstract::With virus-infected autologous and allogenic mononuclear cells as specific targets, the development of cell-mediated cytotoxic reactivity to respiratory syncytial virus (RSV) was studied in peripheral blood lymphocytes (PBL) in groups of infants with acute RSV infection and in other control groups of subjects during a...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890280304

    authors: Chiba Y,Higashidate Y,Suga K,Honjo K,Tsutsumi H,Ogra PL

    更新日期:1989-07-01 00:00:00

  • Virus-induced autoimmunity in hepatitis C virus infections: a rare event.

    abstract::Serial serum samples from 16 Italian patients presenting with acute hepatitis C virus (HCV) infections (which progressed to chronic hepatitis in six) were screened for the non-organ-specific autoantibodies most frequently associated with autoimmune hepatitis (AIH), as well as for antibodies against the hepatic asialog...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890420113

    authors: McFarlane BM,Bridger C,Tibbs CJ,Saleh MG,Fuzio A,Verucchi G,Attard L,Boschi A,Chiodo F,McFarlane IG

    更新日期:1994-01-01 00:00:00

  • SARS-CoV-2 replicating in nonprimate mammalian cells probably have critical advantages for COVID-19 vaccines due to anti-Gal antibodies: A minireview and proposals.

    abstract::Glycoproteins of enveloped viruses replicating in nonprimate mammalian cells carry α-1,3-galactose (α-Gal) glycans, and can bind to anti-Gal antibodies which are abundant in humans. The antibodies have protected humans and their ancestors for millions of years, because they inhibit replication of many kinds of microbe...

    journal_title:Journal of medical virology

    pub_type: 杂志文章,评审

    doi:10.1002/jmv.26312

    authors: Chen JM

    更新日期:2020-07-18 00:00:00

  • Infectibility of separated peripheral blood mononuclear cell subpopulations by varicella-zoster virus (VZV).

    abstract::Varicella zoster-virus (VZV) is a humanpathogenic alpha-Herpesvirus that causes chickenpox after primary infection. The virus spreads by aerosol or direct contact with infectious vesical fluids, it enters the body via the respiratory tract. In a first viremic stage it replicates in local lymph nodes, followed by a sec...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.10323

    authors: Koenig A,Wolff MH

    更新日期:2003-01-01 00:00:00

  • Altered patterns of norovirus GII.b recombinant forms in gastroenteritis outbreaks in Victoria, Australia, 2002-2005 compared to 2006-2011.

    abstract::GII.b norovirus is an obligatory recombinant with an open reading frame (ORF) 1 comprising the GII.b genotype and the ORF 2 region corresponding to one of a number of other norovirus genotypes. GII.b is the second most common genotype after GII.4 associated with gastroenteritis outbreaks in Victoria, Australia. The st...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.23633

    authors: Bruggink LD,Marshall JA

    更新日期:2013-08-01 00:00:00

  • Detection of IgM antibodies to delta antigen after coinfection and superinfection with the delta virus.

    abstract::A sensitive microtitre radioimmunoassay was developed for detection of IgM antibodies to delta antigen. The assay was based on the selective binding of IgM from test sera to antihuman IgM (u-chain specific) fixed to wells of a microtitre plate, and utilized delta antigen extracted from the liver of an experimentally i...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890200403

    authors: Dimitrakakis M,Waters MJ,Wootton A,Gust I

    更新日期:1986-12-01 00:00:00

  • Human cytomegalovirus (HCMV)-specific T cell but not neutralizing or IgG binding antibody responses to glycoprotein complexes gB, gHgLgO, and pUL128L correlate with protection against high HCMV viral load reactivation in solid-organ transplant recipients.

    abstract::Immune correlates of protection against human cytomegalovirus (HCMV) infection are still debated. This study aimed to investigate which arm of the immune response plays a major role in protection against HCMV infection in kidney transplant recipients (n = 40) and heart transplant recipients (n = 12). Overall, patients...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.25225

    authors: Lilleri D,Zelini P,Fornara C,Zavaglio F,Rampino T,Perez L,Gabanti E,Gerna G

    更新日期:2018-10-01 00:00:00

  • Immunity and the burden of herpes zoster.

    abstract::The burden of herpes zoster may be related to patients' immunity, although this has not been studied extensively. This hypothesis was tested in a matched case-control study of patients with herpes zoster who sought treatment at one of seven university hospitals in Korea from January 1, 2007, to December 31, 2010. Pati...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.23830

    authors: Choi WS,Kwon SS,Lee J,Choi SM,Lee JS,Eom JS,Sohn JW,Choeng HJ

    更新日期:2014-03-01 00:00:00

  • Antigenic drift of hemagglutinin and neuraminidase in seasonal H1N1 influenza viruses from Saudi Arabia in 2014 to 2015.

    abstract::Antigenic drift of the hemagglutinin (HA) and neuraminidase (NA) proteins of the influenza virus cause a decrease in vaccine efficacy. Since the information about the evolution of these viruses in Saudi is deficient so we investigated the genetic diversity of circulating H1N1 viruses. Nasopharyngeal aspirates/swabs co...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.25759

    authors: Naeem A,Elbakkouri K,Alfaiz A,Hamed ME,Alsaran H,AlOtaiby S,Enani M,Alosaimi B

    更新日期:2020-03-11 00:00:00