Abstract:
:Individual specific antigenic rubella virus (RV) structural proteins are required for accurate serological diagnosis of acute and congenital rubella infections as well as rubella immune status. The RV envelope glycoprotein E1 is the major target antigen and plays an important role in viral-specific immune responses. The native virion is difficult to produce in large quantities and the protein subunits are also difficult to isolate without loss of antigenicity. The production of a soluble RV E1 (designated E1 delta Tm) using the baculovirus-insect cell expression system is described. In contrast to wild-type RV E1, the genetically engineered E1 delta Tm protein lacks a transmembrane anchor. It behaved as a secretory protein and was secreted abundantly from insect cells. Pulse-chase studies were used to examine the synthesis, glycosylation, and secretion of E1 delta Tm by the insect cells. The secreted E1 delta Tm protein was purified from serum-free medium by one-step immunochromatography. The purified E1 delta Tm protein retained full antigenicity and may be a convenient source of E1 protein for use in diagnostic assay and rubella vaccine development.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Seto NO,Gillam Sdoi
10.1002/jmv.1890440214subject
Has Abstractpub_date
1994-10-01 00:00:00pages
192-9issue
2eissn
0146-6615issn
1096-9071journal_volume
44pub_type
杂志文章abstract::The prevention of mother to child transmission of HIV-1 by zidovudine monotherapy is well known, but increasingly combination anti-retroviral therapy is prescribed during pregnancy. In this prospective study, 19 pregnant women with human immunodeficiency virus-1 (HIV-1) infection who elected to take anti-retroviral th...
journal_title:Journal of medical virology
pub_type: 杂志文章
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abstract::The current Agence Nationale de Recherche sur le SIDA (ANRS)/International AIDS Society (IAS) algorithm predicts resistance to etravirine for viruses harboring >/=3 mutations from a list of 13 reverse transcriptase (RT) mutations. Two weighted algorithms, best correlated with fold changes to etravirine, have been desc...
journal_title:Journal of medical virology
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journal_title:Journal of medical virology
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1999-02-01 00:00:00
abstract::Hepatitis A virus (HAV) is a causative agent of acute viral hepatitis, which represents a significant public health problem. HAV is usually transmitted by oral-fecal route and prevalent not only in developing countries but also in developed countries worldwide. To characterize the HAV wild type strains circulating in ...
journal_title:Journal of medical virology
pub_type: 杂志文章
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更新日期:2008-05-01 00:00:00
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更新日期:2020-06-27 00:00:00
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journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24386
更新日期:2016-04-01 00:00:00
abstract::Nineteen Chinese patients with chronic hepatitis B virus (HBV) infection, seropositive for HBV e antigen (HBeAg) and HBV DNA on at least three occasions in 6 months, were randomised to receive either recombinant human interferon-gamma (rIFN gamma) 0.1 mg/m2 intramuscularly thrice weekly for 16 weeks (n = 11) or no ant...
journal_title:Journal of medical virology
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:1991-07-01 00:00:00
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journal_title:Journal of medical virology
pub_type: 杂志文章
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更新日期:2006-01-01 00:00:00
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journal_title:Journal of medical virology
pub_type: 杂志文章
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更新日期:2013-11-01 00:00:00
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journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(199905)58:1<11::aid-jmv2>3
更新日期:1999-05-01 00:00:00
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journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21153
更新日期:2008-06-01 00:00:00
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journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890420208
更新日期:1994-02-01 00:00:00
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更新日期:2004-08-01 00:00:00
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journal_title:Journal of medical virology
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更新日期:1994-01-01 00:00:00
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