Detection of IgM antibodies to delta antigen after coinfection and superinfection with the delta virus.

Abstract:

:A sensitive microtitre radioimmunoassay was developed for detection of IgM antibodies to delta antigen. The assay was based on the selective binding of IgM from test sera to antihuman IgM (u-chain specific) fixed to wells of a microtitre plate, and utilized delta antigen extracted from the liver of an experimentally infected chimpanzee. This test proved to be useful in distinguishing between coinfection and superinfection with the hepatitis delta virus (HDV). Transient anti-delta IgM responses were observed in patients coinfected with HDV, while prolonged elevated IgM levels were found in HBsAg carriers with chronic liver disease superinfected with HDV. Two distinct serological patterns were observed in both coinfection and superinfection. In coinfection, only 50% of patients with detectable anti-delta IgM went on to develop a long-lasting antibody response. Following superinfection with HDV either stationary or fluctuating levels of IgM antibody were demonstrated. In patients with fluctuating antibody levels, the presence or absence of IgM antibody related to the level of viral replication.

journal_name

J Med Virol

authors

Dimitrakakis M,Waters MJ,Wootton A,Gust I

doi

10.1002/jmv.1890200403

subject

Has Abstract

pub_date

1986-12-01 00:00:00

pages

305-11

issue

4

eissn

0146-6615

issn

1096-9071

journal_volume

20

pub_type

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