Abstract:
:False negativity in a commercial anti-HIV kit (IMx HIV-1/HIV-2 3rd Generation Plus (code 8B32) was investigated, and the kit that superseded it (IMx HIV-1/HIV-2 III Plus, code 8C98) was evaluated. In a comparison on 574 freshly collected anti-HIV-1-positive specimens, 97.2% were more reactive in 8C98 than in 8B32; 35.5% were more than twice as reactive and 8.5% were more than four times as reactive. In 8B32, the signal from 55 specimens selected because of weak reactivity was enhanced 1.5 to 8.8 times by preliminary heating at 56 degrees C for 30 min. The reactivity of the 55 heated sera was then similar to that of the same specimens tested without heat treatment in the 8C98 assay. Reactivity in 8B32 was also increased in 66 of 76 (at least twofold in 20) randomly chosen anti-HIV-positive serum specimens by the addition of EDTA (10 mM final concentration). One of these specimens was false negative (signal:cutoff (S:CO) ratio 0.76) in 8B32, though its reactivity was restored by addition of EDTA (S:CO ratio 9.54). These findings indicate that the inhibitory effect that originally led to false negative findings in 8B32 was probably due to complement activity, and that the same activity was present in the freshly collected specimens used here to evaluate the replacement IMx anti-HIV assay (8C98). The specimen panel employed to evaluate 8C98 included 1,892 anti-HIV-positive and 779 anti-HIV-negative specimens. There were no false negative reactions. The lowest S:CO ratio observed was 6.2 and only 17 (0.2%) anti-HIV-positive specimens gave ratios less than 10. Nine unreproducible false positive reactions arose, all possibly attributable to specimen carryover by the IMx instrument. The performance of 8C98 was also compared with that of 10 other current anti-HIV kits using 21 sets of seroconversion specimens (127 specimens in total), and five performance assessment panels (92 specimens in total) comprised mostly of single bleeds from recent seroconverters. IMx 8C98 was the second most sensitive assay. We found no evidence that the 8C98 kit was prone to the effect that had given rise to false negative results in its predecessor (8B32).
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Parry JV,Perry KR,Harbour S,Burgess C,Mortimer PP,Blackburn NK,Martin Dsubject
Has Abstractpub_date
1998-10-01 00:00:00pages
138-44issue
2eissn
0146-6615issn
1096-9071pii
10.1002/(SICI)1096-9071(199810)56:2<138::AID-JMV7>journal_volume
56pub_type
杂志文章abstract::Several studies have indicated that viral reactivations following allogeneic hematopoietic stem cell transplantation (allo-HSCT) are frequent, but viral reactivations after autologous HSCT (auto-HSCT) have not been investigated in detail. We performed multiplex polymerase chain reaction (PCR) assay to examine multiple...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24621
更新日期:2017-02-01 00:00:00
abstract::The putative interferon sensitivity determining region (ISDR) in the NS5a region of the hepatitis C virus (HCV) was analyzed in 13 interferon alpha (IFN-alpha) treated patients representing genotypes 1a, 1b, and 2b. These patients had previously been followed longitudinally during treatment with respect to viral load ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(199809)56:1<33::aid-jmv6>3
更新日期:1998-09-01 00:00:00
abstract::GB virus C/hepatitis G virus (GBV-C/HGV) RNA was detected by reverse transcription-polymer- ase chain reaction with primers derived from the nonstructural region 3 (NS3) in 9 (4.1%) of 221 blood donors and 2 of 20 (10%) hemophilia patients in Martinique, French West Indies. Anti-E2 antibodies were found in sera from 3...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:1999-10-01 00:00:00
abstract:AIMS:To investigate the association between two RIG-I-like receptor gene polymorphisms and hepatitis C virus (HCV) infection in Chinese Han population. METHODS:The current study genotyped two selected SNPs (IFIH1 rs3747517 and DDX58 rs9695310) using TaqMan allelic discrimination assay to assess their association with ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25398
更新日期:2019-06-01 00:00:00
abstract::One hundred twelve peripheral blood specimens were tested for the presence of cytomegalovirus (CMV) by the tube culture indirect immunoperoxidase (TC-IPA) procedure, the shell vial assay [shell vials were pre- and postinoculation treated with medium containing 2 of 10% fetal bovine serum (FBS) or 100 micrograms% corti...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890380109
更新日期:1992-09-01 00:00:00
abstract::The occurrence of HIV infection among drug-users in Italy between 1990 and 2000 is described; the data concern drug-users attending a nation-wide network consisting of 510 public drug-treatment centres. We included only individuals with written documentation of an HIV-test result. Of the 1,299,972 attendees, 54.5% had...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20054
更新日期:2004-05-01 00:00:00
abstract::A common causative agent for uterine cervical cancer is the human papillomavirus type 18 (HPV-18) which has three phylogenic variants: Asian-Amerindian, European, and African. Each variant shows significant molecular differences in the E6 gene. E6 oncoprotein is a negative regulator of tumor suppressor protein p53, he...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23568
更新日期:2013-07-01 00:00:00
abstract::The various strains of human polyomavirus BK (BKV) show a marked heterogeneity in the non-coding control region (NCCR), which includes the origin of replication and the regulatory region for early and late transcription. A new BKV strain (DDP, U91605) was identified by direct detection and sequencing of PCR products o...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(199908)58:4<413::aid-jmv15
更新日期:1999-08-01 00:00:00
abstract::The incidence of perinatal transmission of hepatitis B virus (HBV) depends on the HBeAg/anti-HBe status of the mother. While children of HBeAg-positive mothers have a 90% probability of acquiring a chronic hepatitis B virus carrier state, babies of anti-HBe-positive mothers are more likely to develop fulminant hepatit...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890470408
更新日期:1995-12-01 00:00:00
abstract::Although analytical treatment interruption is used as a strategy to test immunotherapeutic agents in HIV-infection, it may pose a risk for study participants. The potential risks of short-term interruption of antiretroviral therapy (ART) during treatment with an autologous dendritic cell immune-based therapy (AGS-004-...
journal_title:Journal of medical virology
pub_type: 杂志文章,随机对照试验
doi:10.1002/jmv.23297
更新日期:2012-06-01 00:00:00
abstract::A combined hepatitis A and B vaccine is available since 1996. Two separate open-label primary studies evaluated the immunogenicity and safety of this hepatitis A and B vaccine (720 EI.U of HAV and 20 µg of HBsAg) in 306 healthy subjects aged 17-43 years who received three doses of the vaccine following a 0, 1, and 6 m...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.22264
更新日期:2012-01-01 00:00:00
abstract::Human papilloma virus (HPV) infection is a precursor of cervical cancer. This study aimed to introduce a method to quantify the risk of cervical cancer resulting from infection by different HPV subtypes, to help guide patient treatment. Nucleic acid molecule flow-through hybridization and gene chip technology were use...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24280
更新日期:2015-12-01 00:00:00
abstract::Distinct human papillomavirus (HPV) 18 variants are thought to differ in oncogenic potential and geographic distribution. As such, understanding the regional variants of HPV 18 would be of great importance for evolutionary, epidemiological, and biological analysis. In this regard, the sequence variations of E6 gene we...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26283
更新日期:2020-07-09 00:00:00
abstract:BACKGROUND:Seasonal influenza is an annual occurrence that leads to large community outbreaks and increased hospitalisation. A number of studies have suggested that influenza A (FLUAV) is associated with increased rates of hospitalisation and mortality compared to influenza B (FLUBV). This study compared demographic an...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26600
更新日期:2020-10-15 00:00:00
abstract::The emergence of genotypic resistance in protease and reverse transcriptase (RT) gene regions was longitudinally evaluated in plasma samples from a group of 12 HIV-1-infected patients treated with different combination of antiretroviral therapies and selected on the basis of their clinical failure. Complex mutational ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(199912)59:4<480::aid-jmv10
更新日期:1999-12-01 00:00:00
abstract::The presence of the E2 region of HPV 16 was determined by the polymerase chain reaction in biopsy samples with different histopathological diagnoses. The absence of the E2 gene was observed in 17 of 30 cases examined. The E2 deletions were not confined exclusively to invasive carcinomas or to integrated viral forms. I...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890410102
更新日期:1993-09-01 00:00:00
abstract::The purpose of this study was to determine whether autophagy regulates the expression of human cytomegalovirus (HCMV) immediately early two viral protein (IE2). Rapamycin and 3-methyladenine (3-MA) were used to stimulate or suppress autophagy during HCMV infection. UL122 recombinant plasmid was transfected to overexpr...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26357
更新日期:2020-07-25 00:00:00
abstract::Hepatitis C virus (HCV) core protein has been reported to alter the cell cycle in vitro, but the data remain inconclusive, and in vitro experiments do not represent precisely events that occur in vivo, which may involve hepatic inflammation or regeneration. A group of double-transgenic mice carrying tetracycline trans...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21403
更新日期:2009-03-01 00:00:00
abstract::The replication strategy of a human serotype 1 rotavirus, adapted to rapid growth in CV-1 cells, was investigated. A single cycle growth curve revealed eclipse and latent periods of 3 and 4 hours, respectively. Although the extent of reduction of host cell protein synthesis was directly related to the multiplicity of ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890250113
更新日期:1988-05-01 00:00:00
abstract::Rapid diagnosis of human herpesvirus primary infections or reactivations is facilitated by quantitative PCRs. Quantitative PCR assays with a standard thermal cycling profile permitting simultaneous detection of herpes simplex virus (HSV), varicella zoster virus (VZV), cytomegalovirus (CMV), Epstein-Barr virus (EBV), a...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21095
更新日期:2008-03-01 00:00:00
abstract::Hepatitis E is endemic in India. It was recently noted that although all the Indian human hepatitis E virus (HEV) isolates (1976-2001) were placed in genotype I, the swine HEV recovered from western India (2000) belonged to genotype IV. This was in contrast to reports from the United States and Taiwan wherein both hum...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10301
更新日期:2003-03-01 00:00:00
abstract::Total RNA from normal adult woodchucks was analyzed using membrane arrays containing human cDNA clones, and the gene expression patterns were compared to human liver. Various hybridization and wash conditions were examined. In both the woodchuck and human livers, 352 genes were identified as highly expressed (Z-scores...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20186
更新日期:2004-10-01 00:00:00
abstract::Deletions and insertions in the hepatitis B virus (HBV) X region have been associated with severe forms of liver disease, including hepatocellular carcinoma (HCC). However, the molecular epidemiologic features of this virus have been described rarely. Deletions and insertions in the X region were determined by direct ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21938
更新日期:2011-01-01 00:00:00
abstract::The Balkan Peninsula and Bulgaria in particular, is a well-known endemic region for Crimean-Congo hemorrhagic fever (CCHF). This study describes the prevalence of Crimean-Congo hemorrhagic fever virus (CCHFV) among tick populations from areas, previously recognized with emerging cases of CCHF disease in humans. These ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23214
更新日期:2012-04-01 00:00:00
abstract::Human herpes virus type 6 (HHV-6) DNA has been suggested to be a cofactor to human papillomavirus (HPV) in cervical cancer. In a cross-sectional study, we investigated the association between HHV-6 DNA detected in cervical brushings and high-grade squamous intraepithelial lesions (HSIL), while controlling for genital ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10229
更新日期:2002-12-01 00:00:00
abstract::Persistent infection with high-risk human papillomavirus (HPV) causes cervical preneoplasic lesions and invasive cervical cancer. This study evaluated the prevalence and distribution of HPV genotypes in cervical exfoliated cells from Uruguayan women. Five hundred sixty-eight cervical specimens were examined by PCR usi...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23479
更新日期:2013-05-01 00:00:00
abstract::The aim of this study was to analyze the characteristics of Dengue virus (DENV)-infected children and the accuracy of dengue diagnosis based on clinical presentations. The inclusion criteria were children ≥1-year-old presenting febrile illness with 1-7 days of onset. Children (n = 110) aged 2-15 years were included in...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24533
更新日期:2016-10-01 00:00:00
abstract::Progressive multifocal leukoencephalopathy (PML), a viral-induced demyelinating disease, is becoming relatively common, while many diagnostic and pathogenetic aspects remain to be clarified. A study was therefore undertaken in 64 AIDS patients suffering from various neurological disorders, including PML (12 subjects),...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(199707)52:3<235::aid-jmv1>
更新日期:1997-07-01 00:00:00
abstract::Epstein-Barr virus (EBV) reactivation and cell-mediated immune (CMI) responses were followed in 16 Antarctic expeditioners during winter-over isolation at 2 Australian National Antarctic Research Expedition stations. Delayed-type hypersensitivity (DTH) skin testing was used as an indicator of the CMI response, that wa...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(200006)61:2<235::aid-jmv10
更新日期:2000-06-01 00:00:00
abstract::Epstein-Barr virus (EBV)-associated post-transplant lymphoproliferative disease (PTLD) arises in up to 10% of organ transplant recipients and is fatal in ∼50% of cases. PTLD can be modeled in SCID mice using EBV+ve human B lymphoblastoid cell lines (BLCLs), and the current study investigated intraperitoneal (ip) inocu...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.22164
更新日期:2011-09-01 00:00:00