Improved detection of cytomegalovirus viremia in AIDS patients using shell vial and indirect immunoperoxidase methodologies.

Abstract:

:One hundred twelve peripheral blood specimens were tested for the presence of cytomegalovirus (CMV) by the tube culture indirect immunoperoxidase (TC-IPA) procedure, the shell vial assay [shell vials were pre- and postinoculation treated with medium containing 2 of 10% fetal bovine serum (FBS) or 100 micrograms% cortisol] (SV-IFA), and conventional (MRC-5) tube cultures (TC-CPE). CMV was detected in 25 (22%) of the 112 specimens tested by at least one of these methods. The detection/isolation of CMV among the 25 positive specimens in shell vials maintained with 2% FBS, 100 micrograms% cortisol + 2% FBS, and 10% FBS was 36, 44, and 52%, respectively. Detection/isolation of the virus from blood by TC-IPA and TC-CPE was 52% and 76%, respectively. A significantly greater CMV detection rate occurred using TC-CPE compared to SV-IFA treated with medium supplemented with an FBS concentration of 2% (P = .0132), but not medium containing the higher serum supplement or the glucocorticoid (P greater than .05). Differences in the identification of a CMV viremia were observed by IPA, SV-IFA, and TC-CPE methodologies on a patient-to-patient basis, denoting the necessity of incorporating each methodology into the CMV screening panel. Demographic analysis of 82 AIDS patients showed a CMV viremia prevalence of 9% (2/28) in intravenous drug users, 57% (27/47) in homosexual patients, and 22% (2/9) in heterosexual and transfusion patients. Overnight (24 hr) storage of whole blood at 4 or 24 degrees C, respectively, reduced CMV recovery by 40% and 65%, when tested by TC-CPE.(ABSTRACT TRUNCATED AT 250 WORDS)

journal_name

J Med Virol

authors

Lipson SM,Kaplan MH,Simon JK,Ciamician Z,Tseng LF

doi

10.1002/jmv.1890380109

keywords:

subject

Has Abstract

pub_date

1992-09-01 00:00:00

pages

36-43

issue

1

eissn

0146-6615

issn

1096-9071

journal_volume

38

pub_type

杂志文章
  • Human papillomavirus and mutated H-ras oncogene in cervical carcinomas and pathological negative pelvic lymph nodes: a retrospective follow-up.

    abstract::The metastasis status of pelvic lymph nodes (PLNs) seems to be a predictive factor of survival. It was suggested that the presence of HPV DNA and other biological markers in PLN may indicate a sub clinical early metastasis. The aim was to describe the prevalence and distribution patterns of HPV DNA and H-ras mutations...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21076

    authors: Landro ME,Dalbert D,Picconi MA,Cúneo N,González J,Vornetti S,Bazán G,Mural J,Basiletti J,Teyssié AR,Alonio LV

    更新日期:2008-04-01 00:00:00

  • Altered antibody response to influenza H1N1 vaccine in healthy elderly people as determined by HI, ELISA, and neutralization assay.

    abstract::To determine the influence of ageing per se as well as of priming histories on the antibody response to influenza vaccination, haemagglutination inhibition (HI), ELISA IgG, IgA, IgM and neutralizing antibody titres were studied in 43 healthy young subjects (mean age 23 years) and 55 healthy elderly people (mean age 79...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/(sici)1096-9071(199805)55:1<82::aid-jmv13>

    authors: Remarque EJ,de Bruijn IA,Boersma WJ,Masurel N,Ligthart GJ

    更新日期:1998-05-01 00:00:00

  • Development and validation of a new serum standard for the measurement of anti-HEV antibodies in animals.

    abstract::Although hepatitis E virus (HEV) infection is a zoonosis, there is, currently, no standardized assay for quantitatively measuring anti-HEV antibody levels in HEV animal reservoirs. In this study, anti-HEV antibody positive serum from a rabbit (RS26) was calibrated by dose-response parallel line assay using the World H...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.24651

    authors: Zhang Y,Wang L,Zeng H,Gong W,Wang L,Zhuang H

    更新日期:2017-03-01 00:00:00

  • Seroprevalence of hepatitis A virus antibodies in Turkish and Moroccan children in Rotterdam.

    abstract::Seasonal fluctuations in hepatitis A have been observed in the Netherlands related to Turkish and Moroccan children after visiting their home countries. This study determined the prevalence and associated factors of hepatitis A virus (HAV) antibodies in Turkish and Moroccan children in Rotterdam. A random sample was t...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.10576

    authors: Richardus JH,Vos D,Veldhuijzen IK,Groen J

    更新日期:2004-02-01 00:00:00

  • Fraction-specific populations of the hypervariable region of the hepatitis C virus in a patient with cryoglobulinemia.

    abstract::Nucleotide sequences of the hypervariable region (HVR) of the E2/NS1 gene of hepatitis C virus (HCV), which are now thought to contain epitopes for neutralizing antibodies, were compared between antibody-bound HCV and free HCV in a patient with type II cryoglobulinemia. Antibody-bound HCV was immunoprecipitated with a...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890460418

    authors: Kurosaki M,Enomoto N,Nouchi T,Sakuma I,Marumo F,Sato C

    更新日期:1995-08-01 00:00:00

  • Epidemiology and clinical features of rotavirus, adenovirus, and astrovirus infections and coinfections in children with acute gastroenteritis prior to rotavirus vaccine introduction in Meerut, North India.

    abstract::There are limited reports on the etiology of multiple enteric viruses causing acute gastroenteritis (AGE) in North India. In the present study we have determined the prevalence of three enteric viruses, namely rotavirus, astrovirus (AstV) and adenovirus (AdV) in a total of 312 diarrheic children (<5 years) hospitalize...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.25645

    authors: Akdag AI,Gupta S,Khan N,Upadhayay A,Ray P

    更新日期:2020-08-01 00:00:00

  • Developing a sensitive HBV genotyping assay for HBV DNA suppressed patients using both DNA and RNA sequencing.

    abstract::Hepatitis B virus (HBV) genotypes impact treatment outcomes and disease progression. The current genotyping methods have limitations in patients with low HBV viral load. In this study, a more sensitive assay has been developed for determining the HBV genotype in HBV DNA suppressed patients. Fifty-five serum samples fr...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.26249

    authors: Liu Y,May L,Liu X,Martin R,Svarovskaia E,Gaggar A,Mo H,Feierbach B

    更新日期:2020-07-01 00:00:00

  • Shedding of herpes simplex virus type 1 into saliva in patients with orofacial fracture.

    abstract::Shedding of herpes simplex virus type 1 (HSV-1) into saliva was studied in 83 patients with orofacial fractures. Infectious virus was isolated from 14 of 83 patients (16.8%) with no detectable herpetic lesion during hospitalization. Of the 83 patients, 44 (53%) had HSV-1 specific antibody. Virus shedding into saliva w...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890280205

    authors: Kameyama T,Futami M,Nakayoshi N,Sujaku C,Yamamoto S

    更新日期:1989-06-01 00:00:00

  • The acyclovir legacy: its contribution to antiviral drug discovery.

    abstract::The discovery of acyclovir marked the beginning of an exciting era in antiviral research. Early studies on the novel mode of action explained the selectivity of the compound and the remarkably narrow spectrum of activity against a subset of the herpesviruses. Throughout the past decade many clinical trials have demons...

    journal_title:Journal of medical virology

    pub_type: 杂志文章,评审

    doi:10.1002/jmv.1890410526

    authors: Darby G

    更新日期:1993-01-01 00:00:00

  • Recovery of functional cytotoxic T lymphocytes during lamivudine therapy by acquiring multi-specificity.

    abstract::To characterize cytotoxic T lymphocytes (CTLs) that appeared in circulation during lamivudine therapy, we analyzed HBV-specific CTLs using HLA-A24 tetramer and HBcAg-specific Th1 cells in patients receiving lamivudine therapy. Six patients (HLA-A24(+)) with chronic hepatitis B, six patients (HLA-A24(-)) with chronic h...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20194

    authors: Kondo Y,Asabe S,Kobayashi K,Shiina M,Niitsuma H,Ueno Y,Kobayashi T,Shimosegawa T

    更新日期:2004-11-01 00:00:00

  • Kinetics of HBV DNA and HBsAg in acute hepatitis B patients with and without coinfection by other hepatitis viruses.

    abstract::The kinetics of hepatitis B virus (HBV) and its surface antigen (HBsAg) during acute hepatitis has not yet been studied accurately in a representative number of patients. The influence of coinfecting hepatitis viruses during the acute phase of infection is not known. Three to four serum samples from 21 patients with a...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.10291

    authors: Chulanov VP,Shipulin GA,Schaefer S,Gerlich WH

    更新日期:2003-03-01 00:00:00

  • Personal clothing as a potential vector of respiratory virus transmission in childcare settings.

    abstract::Previous investigations of fomite transmission have focused on the presence of pathogens on inanimate objects in clinical settings. There has been limited investigation of fomite transmission in non-clinical pediatric settings where there is a high prevalence of respiratory virus infections. Over a 5 week period, this...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.24102

    authors: Gralton J,McLaws ML,Rawlinson WD

    更新日期:2015-06-01 00:00:00

  • Allele-specific conventional reverse-transcription polymerase chain reaction as a screening assay for discriminating influenza a H1N1 (H275Y) oseltamivir-resistant and wild-type viruses.

    abstract::In early 2008, a sudden increase in oseltamivir (Tamiflu)-resistant influenza A H1N1 viruses was reported from several European countries. This resistant virus has spread globally and accounted for more than 95% of H1N1 viruses isolated in the following influenza season. A continuous close monitoring on the prevalence...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21783

    authors: Ngai KL,Lam WY,Lee N,Leung TF,Hui DS,Chan PK

    更新日期:2010-08-01 00:00:00

  • Tampon culture for quantitation of cervicovaginal herpes simplex virus.

    abstract::From 10(1) to 10(6) TCID50 (mean tissue culture infective doses) per ml HSV I and II can be quantitatively recorded from vaginal tampons. Tampons and sterile cotton swabs are equally sensitive in recovering HSV I and II. Direct cotton swab cultures of the cervix and cervicovaginal tampon cultures from the same patient...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890020102

    authors: Ekwo EE,Myers MG

    更新日期:1978-01-01 00:00:00

  • Influence of HLA-B18 on liver fibrosis progression in a cohort of HIV/HCV coinfected individuals.

    abstract::Liver fibrosis is accelerated in human immunodeficiency virus/hepatitis C virus (HIV/HCV) coinfected compared with HCV monoinfected patients, due to multiple cofactors. Recently, HLA-B18 haplotype has been associated with short-term liver disease progression in this population. Our aim was to assess the influence of H...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.25385

    authors: Bavaro DF,Saracino A,Fiordelisi D,Bruno G,Ladisa N,Monno L,Angarano G

    更新日期:2019-05-01 00:00:00

  • Junin virus-induced astrocytosis is impaired by iNOS inhibition.

    abstract::Because Junin virus (JV) experimental encephalitis of mice and rats is characterized by mild histopathological changes that do not seem to justify per se lethality after intracerebral infection, such a murine model seems adequate to investigate the potential role of inducible nitric oxide synthase (iNOS) as a pathogen...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.10254

    authors: Gómez RM,Yep A,Schattner M,Berría MI

    更新日期:2003-01-01 00:00:00

  • Norovirus infections and seroprevalence of genotype GII.4-specific antibodies in a Spanish population.

    abstract::Genotype II.4 noroviruses (NoVs) are a leading cause of epidemic acute gastroenteritis in children and adults worldwide. The prevalence of different NoV genotypes causing outbreaks and sporadic cases of acute gastroenteritis in the region of Valencia, Spain, during a 4-year period (2008-11) was investigated. NoVs were...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.24112

    authors: Carmona-Vicente N,Fernández-Jiménez M,Ribes JM,Téllez-Castillo CJ,Khodayar-Pardo P,Rodríguez-Diaz J,Buesa J

    更新日期:2015-04-01 00:00:00

  • Spectrum of hepatitis E virus infection in India.

    abstract::A solid phase enzyme linked immunosorbent assay (ELISA) that detects IgM and IgG to hepatitis E virus (HEV) was used to study seroepidemiology in 40 healthy subjects and 227 consecutive patients with liver diseases in an endemic area. Fifty-two of the liver diseases patients (22.9 percent) had acute hepatitis E. In co...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890430316

    authors: Khuroo MS,Rustgi VK,Dawson GJ,Mushahwar IK,Yattoo GN,Kamili S,Khan BA

    更新日期:1994-07-01 00:00:00

  • Lack of augmenting effect of interferon-gamma on dengue virus multiplication in human peripheral blood monocytes.

    abstract::The effect of interferon-gamma (IFN-gamma) on dengue virus multiplication in human peripheral blood monocytes was investigated. Enriched monocytes were treated with IFN-gamma and then infected with dengue virus type 2 either directly or in the presence of optimal infection-enhancing levels of antibodies. Pretreatment ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890450109

    authors: Sittisombut N,Maneekarn N,Kanjanahaluethai A,Kasinrerk W,Viputtikul K,Supawadee J

    更新日期:1995-01-01 00:00:00

  • Genital herpes: natural history and treatment of acute episodes.

    abstract::Genital herpes is a common, distressing infection whose incidence has been underestimated. Recent serological surveys, employing type-specific antibody assays, show a rising prevalence of previous herpes simplex virus (HSV) type 2 infections in post-adolescent populations in developed countries; many of these infectio...

    journal_title:Journal of medical virology

    pub_type: 杂志文章,评审

    doi:10.1002/jmv.1890410508

    authors: Kinghorn GR

    更新日期:1993-01-01 00:00:00

  • Longitudinal study of plasma HIV-1 RNA concentrations during the asymptomatic stage of HIV infection measured using AMPLICOR HIV monitor and NASBA HIV-1 RNA QT tests. ACCTES Association.

    abstract::The predictive value of two methods for measuring HIV RNA concentration in plasma was assessed in relation to CD4 lymphocyte counts during the asymptomatic period of infection. The design was a retrospective longitudinal case-control study for a mean period of 60 months involving 20 asymptomatic patients included in t...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:

    authors: Delamare C,Burgard M,Deveau C,Chaix ML,Ivanoff S,Ramirez D,Tachet A,Bary M,Rouzioux C

    更新日期:1998-01-01 00:00:00

  • Detection of human cytomegalovirus DNA in perilymph of patients with sensorineural hearing loss using real-time PCR.

    abstract::Although cytomegalovirus (CMV) has been detected in the inner ear fluid of patients who succumbed to the complications of symptomatic congenital CMV infection, it has not been detected in the inner ear fluid of living patients. In this study, real-time polymerase chain reaction (PCR) was used to measure CMV DNA in cli...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.10263

    authors: Sugiura S,Yoshikawa T,Nishiyama Y,Morishita Y,Sato E,Hattori T,Nakashima T

    更新日期:2003-01-01 00:00:00

  • Cervicovaginal synthesis of IgG antibodies to the immunodominant 175-199 domain of the surface glycoprotein gp46 of human T-cell leukemia virus type I.

    abstract::Paired sera, saliva and cervicovaginal secretions from 17 HTLV-I-infected women (19-75 yr) were tested for total IgA and IgG, for IgA and IgG to the immunodominant region gp46/175-Pro-199, for serum IgG to the neutralizing domains gp46/ 190-Pro-199 and gp46/190-Ser-199, or for tax-rex proviral HTLV-DNA. Serum antibodi...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1096-9071(199609)50:1<42::AID-JMV9>3

    authors: Bélec L,Georges-Courbot MC,Georges A,Mohamed AS,Londos-Gagliardi D,Hallouin MC,Hocini H,Guillemain B

    更新日期:1996-09-01 00:00:00

  • High prevalence of clinically unsuspected dengue disease among children in Ribeirao Preto city, Brazil.

    abstract::The aim of this study was to analyze the characteristics of Dengue virus (DENV)-infected children and the accuracy of dengue diagnosis based on clinical presentations. The inclusion criteria were children ≥1-year-old presenting febrile illness with 1-7 days of onset. Children (n = 110) aged 2-15 years were included in...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.24533

    authors: Poloni TR,Dornas FP,Dos Santos NN Jr,Soares AM,Amarilla AA,Alfonso HL,Trigueiro S,Lavrador MA,Yamamoto AY,Aquino VH

    更新日期:2016-10-01 00:00:00

  • Amino acid substitutions in NS5A region of GB virus C and response to interferon therapy.

    abstract::GB virus C (GBV-C) is related to hepatitis C virus (HCV) and has a similar genomic structure. Some predictors for the efficacy of interferon (IFN) therapy on HCV have been reported: genotype, viral load, IFN dose, and the amino acid substitutions in the NS5A region, designated as the interferon sensitivity determining...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/(sici)1096-9071(199904)57:4<376::aid-jmv9>

    authors: Kato T,Mizokami M,Orito E,Ohba K,Nakano T,Kondo Y,Tanaka Y,Ueda R,Mukaide M,Yasuda K,Iino S

    更新日期:1999-04-01 00:00:00

  • Real-life 3D therapy failure: Analysis of NS5A 93H RAS plus 108 K polymorphism in complex with ombitasvir by molecular modeling.

    abstract::We report a real-life 3D therapy failure in a patient treated with ombitasvir (OMV)/paritaprevir/ritonavir and dasabuvir without ribavirin (3D-R). He had therapy failure at week 12 after the end of treatment. We detected resistance-associated substitutions (RASs) plus polymorphisms on NS3, NS5A, and NS5B target region...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.25073

    authors: Marascio N,Pavia G,Romeo I,Talarico C,Di Salvo S,Reale M,Marano V,Barreca GS,Fabiani F,Perrotti N,De Siena M,Giancotti F,Gravina T,Alcaro S,Artese A,Torti C,Liberto MC,Focà A

    更新日期:2018-07-01 00:00:00

  • Swine HEV infection in south India and phylogenetic analysis (1985-1999).

    abstract::Hepatitis E is endemic in India. It was recently noted that although all the Indian human hepatitis E virus (HEV) isolates (1976-2001) were placed in genotype I, the swine HEV recovered from western India (2000) belonged to genotype IV. This was in contrast to reports from the United States and Taiwan wherein both hum...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.10301

    authors: Arankalle VA,Chobe LP,Walimbe AM,Yergolkar PN,Jacob GP

    更新日期:2003-03-01 00:00:00

  • Comprehensive investigation of the presence of JC virus in AIDS patients with and without progressive multifocal leukoencephalopathy.

    abstract::Progressive multifocal leukoencephalopathy (PML), a viral-induced demyelinating disease, is becoming relatively common, while many diagnostic and pathogenetic aspects remain to be clarified. A study was therefore undertaken in 64 AIDS patients suffering from various neurological disorders, including PML (12 subjects),...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/(sici)1096-9071(199707)52:3<235::aid-jmv1>

    authors: Ferrante P,Caldarelli-Stefano R,Omodeo-Zorini E,Cagni AE,Cocchi L,Suter F,Maserati R

    更新日期:1997-07-01 00:00:00

  • Seroprevalence of markers of viral hepatitis in Yemeni healthcare workers.

    abstract::The seroprevalence of viral hepatitis in healthcare workers has important public health implications. To assess the risk factors for the acquisition of viral hepatitis in an unvaccinated cohort from an hyperendemic region, 567 healthcare workers from a large hospital in the capital of the Republic of Yemen were interv...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20126

    authors: Shidrawi R,Ali Al-Huraibi M,Ahmad Al-Haimi M,Dayton R,Murray-Lyon IM

    更新日期:2004-08-01 00:00:00

  • A randomised controlled trial of recombinant interferon-gamma in Chinese patients with chronic hepatitis B virus infection.

    abstract::Nineteen Chinese patients with chronic hepatitis B virus (HBV) infection, seropositive for HBV e antigen (HBeAg) and HBV DNA on at least three occasions in 6 months, were randomised to receive either recombinant human interferon-gamma (rIFN gamma) 0.1 mg/m2 intramuscularly thrice weekly for 16 weeks (n = 11) or no ant...

    journal_title:Journal of medical virology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1002/jmv.1890340310

    authors: Lau JY,Lai CL,Wu PC,Chung HT,Lok AS,Lin HJ

    更新日期:1991-07-01 00:00:00