Abstract:
:The emergence of genotypic resistance in protease and reverse transcriptase (RT) gene regions was longitudinally evaluated in plasma samples from a group of 12 HIV-1-infected patients treated with different combination of antiretroviral therapies and selected on the basis of their clinical failure. Complex mutational patterns in the reverse transcriptase gene were observed. In particular, combinations of AZT (41L, 67N, 70R, 210W, and 219Q/E) and 3TC (184M) were seen in 10 patients. Two patients presented codon 151 multinucleoside analogue resistance (MNR). Additionally, seven patients harbored RT nonnucleoside analogue-related resistance substitutions (98G, 103N, and 181C). Multiple protease-selected mutations were found in each patient with an average of six substitutions per patient, with 10I/F/V, 63P, 71V, 82A/T, 84V, and 90M being the most prevalent substitutions. Overall, these results showed that for most patients virological failure was coupled with detectable genotypic resistance. Furthermore, most patients exhibited genotypic resistance to almost all available anti-HIV-1 drugs. The high viral loads found in most patients at the end of the study suggest that the replication of these multidrug resistant viruses are not severely compromised. Phylogenetic analysis of these pol sequences revealed that a specific HIV-1 genotype prone to develop multidrug resistance was not found.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Cabana M,Clotet B,Martínez MAdoi
10.1002/(sici)1096-9071(199912)59:4<480::aid-jmv10keywords:
subject
Has Abstractpub_date
1999-12-01 00:00:00pages
480-90issue
4eissn
0146-6615issn
1096-9071pii
10.1002/(SICI)1096-9071(199912)59:4<480::AID-JMV10journal_volume
59pub_type
杂志文章abstract::Mitochondrial DNA (mtDNA) of peripheral blood mononuclear cells (PBMCs) collected from Human immunodeficiency virus 1 (HIV-1)-infected patients and healthy controls were measured longitudinally using real-time polymerase chain reaction to evaluate the effects of antiretroviral agents on mtDNA synthesis in vivo and to ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10423
更新日期:2003-08-01 00:00:00
abstract::A sensitive microtitre radioimmunoassay was developed for detection of IgM antibodies to delta antigen. The assay was based on the selective binding of IgM from test sera to antihuman IgM (u-chain specific) fixed to wells of a microtitre plate, and utilized delta antigen extracted from the liver of an experimentally i...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890200403
更新日期:1986-12-01 00:00:00
abstract:BACKGROUND AND AIM:Genotype 3b hepatitis C virus (HCV) infection represents approximately 50% of patients with genotype 3 in China. We compared the efficacy of sofosbuvir (SOF) plus ribavirin (RBV) in Chinese patients with genotype 3a and 3b HCV. METHODS:The analyzed data are from a phase 3, open-label study of SOF pl...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25454
更新日期:2019-07-01 00:00:00
abstract::Fluorescent in situ hybridization (FISH) was used to investigate the chromosomal integration sites of human herpesvirus 6 (HHV-6) in phytohemagglutinin-stimulated leukocytes and B lymphocytes from Epstein-Barr virus transformed lymphoblastoid cell lines (LCLs). Five different chromosomal integration sites were found i...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21299
更新日期:2008-11-01 00:00:00
abstract::Reactivation of hepatitis B e antigen (HBeAg) negative chronic hepatitis B virus (HBV) infection due to selection of precore variant virus is an uncommon complication of previous hepatitis B infection, and virtually unrecognised in children and adolescents. A child who had received treatment with methylprednisolone an...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:2001-11-01 00:00:00
abstract::The severe acute respiratory syndrome (SARS) leads to severe injury in the lungs with multiple factors, though the pathogenesis is still largely unclear. This paper describes the particular analyses of the transcriptome of human lung tissue that was infected by SARS-associated coronavirus (SARS-CoV). Random primers we...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.22012
更新日期:2011-04-01 00:00:00
abstract::Enzyme immunoassay (EIA) for parainfluenza virus type 1, 2, and 3 antibodies was compared with the complement fixation test (CF) as a diagnostic method in 180 patients with respiratory symptoms. The CF test detected rises in parainfluenza virus antibodies in 30 cases, whereas EIA detected 47 rises. Patients with antib...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890140212
更新日期:1984-01-01 00:00:00
abstract::The ability of the anti-cancer drug, 9-Nitrocamptothecin (9NC), to inhibit replication of HIV-1 in clinically relevant primary lymphocytic cells was studied. Primary peripheral blood lymphocytes (PBLs) from a non-infected donor were freshly infected with HIV-1 and treated with 9NC by using three different treatment sc...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1042
更新日期:2001-07-01 00:00:00
abstract::Little information about immunity to respiratory syncytial virus (RSV) and disease pathogenesis in elderly persons exists. Humoral immunity to RSV was assessed in 41 young, 56 healthy elderly, and 49 frail elderly adults by measuring baseline RSV specific IgG by enzyme immunoassay (EIA) and microneutralization assay (...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(199910)59:2<221::aid-jmv16
更新日期:1999-10-01 00:00:00
abstract::Avidity maturation of IgG antibody responses directed against the structural proteins of rubella virus (E1, E2, and C) as well as whole rubella virus (RV) was assessed at sequential time intervals in 7 individuals following serologically confirmed wild rubella infection. Individual structural proteins were purified fr...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890360310
更新日期:1992-03-01 00:00:00
abstract::SARS-COV-2 (COVID-19) is a novel virus that has caused over 28 million cases worldwide and over 900,000 deaths since early 2020, rightfully being classified as a pandemic. COVID-19 is diagnosed via PCR testing which looks at cycle threshold values of two genes, N2 and E. This study examined cycle threshold values of C...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26835
更新日期:2021-02-02 00:00:00
abstract::Self-limited involvement of the central nervous system (CNS) is a relatively common complication of primary infection with human herpesvirus six (HHV-6) in normal children. We describe an HIV-infected infant who developed fulminant encephalitis as a complication of HHV-6 infection. Immunohistochemical staining of CNS ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890450309
更新日期:1995-03-01 00:00:00
abstract::Prophylactic antiviral therapy is recommended for hepatitis B virus (HBV)-infected patients with malignancies who are undergoing systemic chemotherapy. In the current study, we aimed to develop a risk scoring system to guide the selection of prophylactic antiviral agents. In this retrospective analysis, we included co...
journal_title:Journal of medical virology
pub_type: 杂志文章,多中心研究
doi:10.1002/jmv.25241
更新日期:2018-10-01 00:00:00
abstract::Anal cancer is a rare disease. Nevertheless, it may be a reason for concern among groups in which its incidence is increasing: those who engage in anoreceptive intercourse, promiscuous persons, and those with sexually transmitted infections (HPV and HIV). The aim of this study was to evaluate the prevalence of abnorma...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23346
更新日期:2012-09-01 00:00:00
abstract::Occult HBV infection is defined as the persistence of HBV DNA in individuals negative for HBV surface antigen (HBsAg), and many different mechanisms have been reported in different countries. However, in China, one of the endemic areas for HBV infection, no reports have been published on occult HBV infection. The pres...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21463
更新日期:2009-05-01 00:00:00
abstract::Noroviruses (NoVs) are a leading cause of acute gastroenteritis outbreaks and sporadic cases of diarrhea in industrialized countries. To study the prevalence and genetic diversity of NoVs in Guatemala, stool specimens were collected from hospitalized and ambulatory patients presenting with diarrhea (≥3 loose or liquid...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23578
更新日期:2013-07-01 00:00:00
abstract::Dual infection with hepatitis B and C viruses is often encountered in endemic areas of both viruses. However, understanding of the clinical and virological implications is limited. The aim of this study was to investigate the role of each virus in liver injury and the interaction between the two viruses in dual infect...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890460316
更新日期:1995-07-01 00:00:00
abstract::Enterovirus (EV) infections are common. There are more than 60 known serotypes, and each has different epidemiologic or medical importance. Over 700 physicians from 75% of basic administrative units of Taiwan participated in the "Sentinel Physician Surveillance of Infectious Disease" and reported weekly to the Center ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21006
更新日期:2007-12-01 00:00:00
abstract::Rotavirus-specific subclass antibody responses and cytokines, tumour necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), interleukin-8 (IL-8), and IL-10, were measured in children 7-24 months of age with rotavirus diarrhoea (n = 29); the responses were compared with children with watery diarrhoea from who...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10280
更新日期:2003-02-01 00:00:00
abstract::There is an obvious concern globally regarding the fact about the emerging coronavirus 2019 novel coronavirus (2019-nCoV) as a worldwide public health threat. As the outbreak of COVID-19 causes by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) progresses within China and beyond, rapidly available epi...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25743
更新日期:2020-06-01 00:00:00
abstract::This study aimed to investigate the characteristics of female high-risk human papillomavirus (HR-HPV) infection in Shaanxi province of China. A total of 14 111 women were enrolled for HPV genotyping test, and a cytology, and/or cervix biopsy were performed in partial women. Of these women, the HPV infection rate was 3...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24748
更新日期:2017-07-01 00:00:00
abstract::The impact of cytomegalovirus (CMV) infection of the genital tract during pregnancy on adverse pregnancy outcomes is not understood fully. A real-time PCR assay was used to determine vaginal shedding of CMV in 993 healthy pregnant Japanese women and the results were compared with the outcome of pregnancy. CMV DNA was ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20619
更新日期:2006-06-01 00:00:00
abstract::To design a safe and effective synthetic peptide vaccine against rubella virus (RV) infection, it is necessary to identify immunodominant T-cell epitopes of RV structural proteins. To define such epitopes, 49 overlapping synthetic peptides (17-34 residues in length) corresponding to more than 95% of the amino acid seq...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890400302
更新日期:1993-07-01 00:00:00
abstract::Crimean-Congo hemorrhagic fever (CCHF) is an infectious disease that is caused by CCHF virus. A family of transmembrane receptors called as Toll-like receptors (TLRs) selectively acts in recognizing a wide range of microbial components and endogenous molecules released by damaged tissue and have been preserved through...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24519
更新日期:2016-10-01 00:00:00
abstract::Human astroviruses (HAstVs) have been recognized as one of the major causes of acute gastroenteritis in children. To provide more insight into the prevalence of HAstV gastroenteritis in China, 664 fecal samples were collected from children affected with acute gastroenteritis in Beijing from March 2005 to November 2007...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21729
更新日期:2010-03-01 00:00:00
abstract::The aetiological agents responsible for, and the outcome of, acute liver failure were investigated prospectively in 44 children (29 males, 15 females) attending a tertiary health care facility in India. The children were between the ages of 2 months and 13 years. Studies for viral infections and other etiologies could...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(SICI)1096-9071(199603)48:3<215::AID-JMV1>
更新日期:1996-03-01 00:00:00
abstract::Influenza A/H3N2 viruses are associated with severe epidemics. Antiviral resistance and continued antigenic drift are the major concerns regarding prophylaxis and treatment of influenza. The objectives of this study were to investigate the prevalence and frequency of antiviral drug resistance in influenza A/H3N2 virus...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23854
更新日期:2014-06-01 00:00:00
abstract::In vitro, beta-herpesviruses can stimulate or inhibit HIV replication under particular circumstances. In order to investigate the effects of beta-herpesvirus infection on HIV replication and vice versa at an organ level, we determined the quantitative relationships between cytomegalovirus (CMV), human herpesviruses (H...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(199903)57:3<278::aid-jmv11
更新日期:1999-03-01 00:00:00
abstract::Five HIV-1 isolates were obtained sequentially from a single seropositive individual during the later stages of AIDS. Four of these isolates were adapted to grow in a continuous human T-lymphocytic cell line. Comparative biological and biochemical studies of the virus isolates were made using persistently infected cul...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890340207
更新日期:1991-06-01 00:00:00
abstract::Physicochemical and molecular characterization studies of hepatitis C virus (HCV), the major causative agent of parenterally transmitted non-A, non-B hepatitis (PT-NANBH), strongly suggest that it is a pesti-/flavivirus-like virus. Additional studies show that the buoyant density of plasma-derived HCV in sucrose is si...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890340315
更新日期:1991-07-01 00:00:00