Abstract:
:To design a safe and effective synthetic peptide vaccine against rubella virus (RV) infection, it is necessary to identify immunodominant T-cell epitopes of RV structural proteins. To define such epitopes, 49 overlapping synthetic peptides (17-34 residues in length) corresponding to more than 95% of the amino acid sequence of RV virion proteins E1 (23 peptides) and C (11 peptides) and all of E2 (15 peptides) were synthesized and tested for their capacities to induce proliferative responses of rubella-specific T-cell lines and T-cell clones derived from 4 study groups (5 women infected with RV in pregnancy, 5 patients with congenital rubella syndrome, 5 seropositive healthy donors, and 5 RV vaccine recipients). The most frequently recognized epitopes were E1-21 (residues 358-377) with 11/20 responders, E2-4 (residues 54-74) with 6/20 responders, and C11 (residues 255-280) with 11/20 responders, respectively. E1-10 (residues 174-193), E1-16 (residues 272-291) and E1-18 (residues 307-326) were responded to strongly by corresponding T-cell clones, and were recognized by 4 or 5 T-cell lines. T-cell lines derived from three congenital rubella syndrome patients did not respond to any of the synthetic peptides. The results showed that more T-cell epitopes were present in E1 (19/23) and C (10/11) than in E2 (8/15). The identification of T cell sites recognized frequently by RV-infected or -immunized populations could provide the basis for selecting candidate T-cell epitopes for the development of an effective synthetic vaccine against rubella.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Ou D,Chong P,Tingle AJ,Gillam Sdoi
10.1002/jmv.1890400302subject
Has Abstractpub_date
1993-07-01 00:00:00pages
175-83issue
3eissn
0146-6615issn
1096-9071journal_volume
40pub_type
杂志文章abstract::Cervical cancer is the second most common cancer among Tunisian women, and the incidence rates vary by region. Three Tunisian registries report age-standardized rates of 6.3/10(5) in the central region, 5.4/10(5) in the north, and 2.7/10(5) in the south. High-risk human papillomavirus (HPV) types and their variants di...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.22011
更新日期:2011-04-01 00:00:00
abstract::An anti-hepatitis A virus (HAV)-specific immunoglobulin M capture enzyme-linked immunosorbent assay (anti-HAV IgM ELISA) kit was re-designed for laboratory use and compared with a commercial anti-HAV IgM detection system using 58 serum samples collected from patients, vaccines, and healthy individuals. Because concord...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21578
更新日期:2009-09-01 00:00:00
abstract::Interleukin 6 (IL-6) is a pleiotropic cytokine with pivotal functions in the regulation of the biological responses of several target cells, including hepatocytes. Previous studies have shown that serum IL-6 levels are increased in hepatitis B patients. However, the role of IL-6 in modulating the anti-hepatitis B viru...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25382
更新日期:2019-05-01 00:00:00
abstract::The objective of this study was to determine the use of immune-complex dissociated (ICD) p24 antigen detection for the diagnosis and prognosis of HIV-1 infection in Ugandan children. Plasma collected prospectively from children born to HIV-1 infected Ugandan women was stored and later analyzed for the presence of neut...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/1096-9071(200012)62:4<426::aid-jmv6>3.0.co
更新日期:2000-12-01 00:00:00
abstract::Within the framework of a comprehensive study of the correlation between enteric diseases and wastewater utilization in agricultural settlements (kibbutzim) the prevalence of several viral antibodies was examined among kibbutz residents and overseas volunteers. The latter were assumed to be a group highly susceptible ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890200211
更新日期:1986-10-01 00:00:00
abstract::Thirty susceptible rhesus monkeys were inoculated with cell-free varicella-zoster virus strain OKA or strain KMcC. Both wild and attenuated strains were used. No clinical signs characteristic of human varicella were seen in any of the animals. Virus was not isolated from throat swabs, blood, or cerebrospinal fluid. An...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890140403
更新日期:1984-01-01 00:00:00
abstract::Human metapneumovirus (hMPV) is a pathogen of the respiratory tract with a worldwide distribution. The purpose of this study was to identify hMPV as the cause of acute respiratory diseases in children admitted at Spedali Civili, a public hospital in Brescia, Italy. Eight hundred forty-six nasopharyngeal aspirate sampl...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23213
更新日期:2012-03-01 00:00:00
abstract::The increased immigration from developing regions to Western countries raises public health concerns related to blood-borne viruses. The prevalence of human immunodeficiency virus (HIV), hepatitis B virus (HBV), hepatitis C virus (HCV), and human T-lymphotropic virus (HTLV) infections among recent immigrants attending...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20744
更新日期:2006-12-01 00:00:00
abstract::The seroprevalence of viral hepatitis in healthcare workers has important public health implications. To assess the risk factors for the acquisition of viral hepatitis in an unvaccinated cohort from an hyperendemic region, 567 healthcare workers from a large hospital in the capital of the Republic of Yemen were interv...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20126
更新日期:2004-08-01 00:00:00
abstract::To elucidate the relationship between the clinical severity of chronic liver disease and the precore mutations in hepatitis B e antigen (HBeAg)-negative hepatitis B virus (HBV) carriers, mutations were investigated in the precore region of HBV DNA in 20 chronic hepatitis B patients who seroconverted either spontaneous...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890450404
更新日期:1995-04-01 00:00:00
abstract::A novel simultaneous detection system for human viruses was developed using a real-time polymerase chain reaction (PCR) system to identify causes of infection in clinical samples from patients with uncertain diagnoses. This system, designated as the "multivirus real-time PCR," has the potential to detect 163 human vir...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21962
更新日期:2011-02-01 00:00:00
abstract::The major risk factor for intrauterine transmission of human cytomegalovirus (HCMV) is a primary infection during pregnancy. The neutralizing antibody response appeared after an average of 13 weeks after seroconversion and therefore the absence of neutralizing titers in HCMV IgG positive pregnant women is a reliable m...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:2001-02-01 00:00:00
abstract::In order to determine whether peripheral blood mononuclear cells (PBMC) contain proviral DNA or double-stranded replicative forms of TT virus (TTV) and to define which cell subset harbors TTV, we analyzed 126 PBMC DNA samples by PCR, using the NG059/NG061/NG063 and the T801/T935 primer sets. TTV sequences were detecte...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.2142
更新日期:2002-02-01 00:00:00
abstract::A patient presented with acute retinal necrosis of the left eye. Demonstration of herpes simplex virus (HSV) DNA in the aqueous humour confirmed the diagnosis. Negative results of HSV type-specific antibody tests based on gG antigens suggested a primary HSV infection. However, the patient had a past history of laborat...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20083
更新日期:2004-06-01 00:00:00
abstract::One hundred fifty-five chronic hepatitis C patients were assigned at random to receive natural lymphoblastoid interferon (IFN)alpha-n1, s.c., for 13 months in one of three treatment regimens: initial daily induction with 10 million units (MU) followed (group 1, n = 50) or not (group 2, n = 52) by 1 month of rest and t...
journal_title:Journal of medical virology
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1002/jmv.1072
更新日期:2001-08-01 00:00:00
abstract::Infection with putative non-A to E hepatitis virus, designated GB virus C (GBV-C), was surveyed in 286 patients with chronic liver disease in Japan. RNA of GBV-C was detected, by reverse-transcription polymerase chain reaction with nested primers from the 5'-noncoding region, in 19 patients (6.6%) at a frequency highe...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:1997-03-01 00:00:00
abstract::Dried blood spots collected on filter paper are considered potential clinical specimens for measles surveillance because of their ease of collection, storage, and transport. The usefulness of these samples for surveillance of measles was evaluated in a field setting. Blood spots were collected by finger-prick from 316...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20136
更新日期:2004-08-01 00:00:00
abstract::In vitro, beta-herpesviruses can stimulate or inhibit HIV replication under particular circumstances. In order to investigate the effects of beta-herpesvirus infection on HIV replication and vice versa at an organ level, we determined the quantitative relationships between cytomegalovirus (CMV), human herpesviruses (H...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(199903)57:3<278::aid-jmv11
更新日期:1999-03-01 00:00:00
abstract::In early 2008, a sudden increase in oseltamivir (Tamiflu)-resistant influenza A H1N1 viruses was reported from several European countries. This resistant virus has spread globally and accounted for more than 95% of H1N1 viruses isolated in the following influenza season. A continuous close monitoring on the prevalence...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21783
更新日期:2010-08-01 00:00:00
abstract::Group C rotavirus (RVC) is potentially an important pathogen associated with acute gastroenteritis (AG), especially in outbreaks. This study aims to detect and molecularly characterize RVC in hospitalized children with AG in Belém, Brazil. From May 2008 to April 2011, 279 stools were subjected to reverse-transcription...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24379
更新日期:2016-04-01 00:00:00
abstract::Influenza epidemic is an important cause of severe illness in the elderly. Age-dependent morbidity of influenza in the elderly is associated with weakened immunity. The baseline age-related memory T cell activity in Chinese persons who are exposed to influenza virus through natural infection, are associated with the p...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21544
更新日期:2009-08-01 00:00:00
abstract::The prevalence rate of IgG antibodies to cytomegalovirus (CMV) was determined in a sample of 567 women of childbearing age in the southern part of Israel by the immunoperoxidase assay to membrane antigen (IPAMA) technique. Urban Jewish women of Afro-Asian origin showed significantly higher rates of seropositivity than...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890240104
更新日期:1988-01-01 00:00:00
abstract::The ability of dengue virus-infected human monocyte-derived macrophages to induce permeability changes in primary human umbilical vein endothelial cells was investigated. Supernatants from dengue virus type 2-infected monocyte-derived macrophages increased permeability in human umbilical vein endothelial cell monolaye...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10340
更新日期:2003-04-01 00:00:00
abstract::Chronic papillary conjunctivitis has been described following adenoviral conjunctivitis. It is unknown however, how long adenovirus is able to persist in the tear film and conjunctiva. To determine if adenovirus persists in the ocular surface following adenoviral conjunctivitis, 304 patients with a history of adenovir...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20440
更新日期:2005-10-01 00:00:00
abstract::Hepatitis E is endemic in India. It was recently noted that although all the Indian human hepatitis E virus (HEV) isolates (1976-2001) were placed in genotype I, the swine HEV recovered from western India (2000) belonged to genotype IV. This was in contrast to reports from the United States and Taiwan wherein both hum...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10301
更新日期:2003-03-01 00:00:00
abstract::Genotype 1F was likely localized geographically to China as it has not been reported elsewhere. In this study, whole genome sequences of two rubella 1F virus isolates were completed. Both viruses contained 9,761 nt with a single nucleotide deletion in the intergenic region, compared to the NCBI rubella reference seque...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23936
更新日期:2014-12-01 00:00:00
abstract::Strains of human coronavirus (HCV) isolated between 1974 and 1976 have been studied in vitro and in volunteers. All strains caused colds in volunteers, and those cultivable in tissue culture (TC) produced significantly more coryza and less sore throat than strains growing only in organ culture (OC). The TC strains wer...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890130208
更新日期:1984-01-01 00:00:00
abstract::During 2002, an upsurge in frequency of hepatitis A outbreaks among injecting drug users was observed in England and Wales. As lack of risk factor information and the high mobility of the cases made linkage of outbreaks difficult, the relationship of nucleotide sequences in the VP1/2PA junction of the hepatitis A viru...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21207
更新日期:2008-07-01 00:00:00
abstract::Sexually transmitted disease (STD) remains a major public health challenge in developed countries, exacerbated by the advent of the HIV epidemic. The objectives of this study were to assess the prevalence of serological markers of syphilis, HIV-1/2, HTLV-I/II, HBV, and HCV infections among immigrant sex workers in Mad...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20208
更新日期:2004-12-01 00:00:00
abstract::Serum levels of interleukin-6 (IL-6) are elevated in acute and chronic hepatitis B patients. The effect of IL-6 and its transcription factor of NF-IL6 (a nuclear factor for IL-6) on hepatitis B virus (HBV) enhancer 1 (Enh1), which controls HBV X expression, were investigated in HepG2 cells. Twenty ng/ml of IL-6 increa...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(199708)52:4<413::aid-jmv12
更新日期:1997-08-01 00:00:00