Abstract:
:In order to determine whether peripheral blood mononuclear cells (PBMC) contain proviral DNA or double-stranded replicative forms of TT virus (TTV) and to define which cell subset harbors TTV, we analyzed 126 PBMC DNA samples by PCR, using the NG059/NG061/NG063 and the T801/T935 primer sets. TTV sequences were detected in nearly 20% (25/126) of PBMC DNA samples. Analysis of PBMC subsets revealed that TTV was preferentially distributed in CD19(+) cells. TTV DNA was also detected in CD3(+)/CD19(+) double-depleted cells but was undetectable in CD3(+) cells. After degradation of single-stranded DNA using mung bean nuclease (MBN), TTV could not be detected in previously TTV-positive PBMC DNA samples nor in DNA from CD19(+) cells, CD3(+)-depleted, and CD3(+)/CD19(+) double-depleted cells, indicating the lack of TTV DNA integration and the absence of viral double-stranded replicative intermediates in PBMC or PBMC subset cells. Our data indicate that PBMC and PBMC subsets are not the major sites of TTV replication.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Yu Q,Shiramizu B,Nerurkar VR,Hu N,Shikuma CM,Melish ME,Cascio K,Imrie A,Lu Y,Yanagihara Rdoi
10.1002/jmv.2142keywords:
subject
Has Abstractpub_date
2002-02-01 00:00:00pages
276-84issue
2eissn
0146-6615issn
1096-9071pii
10.1002/jmv.2142journal_volume
66pub_type
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