Molecular characterization and functional analysis of occult hepatitis B virus infection in Chinese patients infected with genotype C.

Abstract:

:Occult HBV infection is defined as the persistence of HBV DNA in individuals negative for HBV surface antigen (HBsAg), and many different mechanisms have been reported in different countries. However, in China, one of the endemic areas for HBV infection, no reports have been published on occult HBV infection. The present study investigated the virological features and the mechanism of occult HBV infection in China. Full-length HBV DNA from eight patients with occult HBV infection (S1-S8) and three HBsAg-positive cases (SWT1-SWT3) was cloned and sequenced. Additionally, four entire linear HBV genomes from occult cases were transfected transiently into HepG2 cells. The sequencing results showed two major mutations in patients with occult HBV infection as follows: deletions in the pre-S1 (S3, S4, and S7) and X (S1, S2, and S5) regions. Such deletions covered the S promoter and the basal core promoter (BCP), and function analysis of these variants also showed a decrease in DNA replication and antigen expression. Two patients with occult infection (S6 and S8) had no mutations capable of interfering with viral replication and gene expression in the major viral population. Thus, the deletions in the S promoter and the BCP regions that disable the regulatory elements may be the reason for the absence of HBsAg, and multiple mechanisms may be responsible for occult HBV infection.

journal_name

J Med Virol

authors

Fang Y,Teng X,Xu WZ,Li D,Zhao HW,Fu LJ,Zhang FM,Gu HX

doi

10.1002/jmv.21463

subject

Has Abstract

pub_date

2009-05-01 00:00:00

pages

826-35

issue

5

eissn

0146-6615

issn

1096-9071

journal_volume

81

pub_type

杂志文章
  • Acute retinal necrosis six years after herpes simplex encephalitis: an elusive immune deficit suggested by insufficient test sensitivity.

    abstract::A patient presented with acute retinal necrosis of the left eye. Demonstration of herpes simplex virus (HSV) DNA in the aqueous humour confirmed the diagnosis. Negative results of HSV type-specific antibody tests based on gG antigens suggested a primary HSV infection. However, the patient had a past history of laborat...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20083

    authors: Preiser W,Doerr HW,Buxbaum S,Rabenau HF,Baatz H

    更新日期:2004-06-01 00:00:00

  • HCV and HIV co-infection in pregnant women attending St. Camille Medical Centre in Ouagadougou (Burkina Faso).

    abstract::Five hundred and forty-seven pregnant women with less than 32 weeks of amenorrhoea, attending an antenatal clinic of St. Camille Medical Centre (SCMC) of Ouagadougou were enrolled for a hepatitis C virus (HCV) and HIV co-infection study. Fifty-eight (10.6%) were HIV positive and 18 (3.3%) were anti-HCV positive. Only ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20258

    authors: Simpore J,Ilboudo D,Samandoulougou A,Guardo P,Castronovo P,Musumeci S

    更新日期:2005-02-01 00:00:00

  • Emergence of the GII4/2006b variant and recombinant noroviruses in China.

    abstract::Noroviruses are an important cause of acute gastroenteritis. Increasing data showed that the GII-4 strains are predominant worldwide and new GII-4 variants emerge every 1-2 years causing major epidemics. Surveillance of gastroenteritis in hospitalized children under 5 years of age in China is described. Among 1,110 sp...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21308

    authors: Jin M,Xie HP,Duan ZJ,Liu N,Zhang Q,Wu BS,Li HY,Cheng WX,Yang SH,Yu JM,Xu ZQ,Cui SX,Zhu L,Tan M,Jiang X,Fang ZY

    更新日期:2008-11-01 00:00:00

  • Complement-mediated cytolytic activity associated with cytomegalovirus complement-fixing IgM antibody.

    abstract::Cytomegalovirus-specific complement-fixing IgM (CMV CF-IgM) antibody was detected in 12/84 (14.3 percent) sera containing CMV-specific IgM. Higher titres were obtained by using a purified antigen preparation containing enveloped virus particles and membrane fragments when compared with a crude extract of infected cell...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890240312

    authors: Booth JC,Mohammed HS

    更新日期:1988-03-01 00:00:00

  • Sequence and structural analysis of the 5' noncoding region of hepatitis C virus in patients with chronic infection.

    abstract::Hepatitis C virus (HCV), exhibits considerable genetic diversity, but presents a relatively well conserved 5' noncoding region (5' NCR) among all genotypes. In this study, the structural features and translational efficiency of the HCV 5' NCR sequences were analyzed using the programs RNAfold, RNAshapes and RNApdist a...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21507

    authors: Araújo FM,Machado-Lima A,Durham AM,Teixeira R,Oliveira G

    更新日期:2009-07-01 00:00:00

  • Valomaciclovir versus valacyclovir for the treatment of acute herpes zoster in immunocompetent adults: a randomized, double-blind, active-controlled trial.

    abstract::Herpes zoster is a common infectious disease that can result in significant acute and chronic morbidity. The safety and efficacy of once-daily oral valomaciclovir (EPB-348) was evaluated for non-inferiority to 3-times daily valacyclovir, an approved therapy. In this study, 373 immunocompetent adults with onset of a he...

    journal_title:Journal of medical virology

    pub_type: 杂志文章,多中心研究,随机对照试验

    doi:10.1002/jmv.23329

    authors: Tyring SK,Plunkett S,Scribner AR,Broker RE,Herrod JN,Handke LT,Wise JM,Martin PA,Valomaciclovir Zoster Study Group.

    更新日期:2012-08-01 00:00:00

  • Diagnosis of COVID-19 pneumonia despite missing detection of viral nucleic acid and initially inconspicuous radiologic findings.

    abstract::The diagnosis of coronavirus disease 2019 (COVID-19) is mainly based on a positive severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) polymerase chain reaction (PCR) result. PCR samples are obtained from upper or lower respiratory tract specimens. However, the sensitivity of PCR is known to have some limitat...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.26153

    authors: Schiller M,Wydra S,Kerl HU,Kick W

    更新日期:2020-11-01 00:00:00

  • Genomic analysis of the Chinese genotype 1F rubella virus that disappeared after 2002 in China.

    abstract::Genotype 1F was likely localized geographically to China as it has not been reported elsewhere. In this study, whole genome sequences of two rubella 1F virus isolates were completed. Both viruses contained 9,761 nt with a single nucleotide deletion in the intergenic region, compared to the NCBI rubella reference seque...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.23936

    authors: Zhu Z,Chen MH,Abernathy E,Zhou S,Wang C,Icenogle J,Xu W

    更新日期:2014-12-01 00:00:00

  • Molecular identification of a novel human rotavirus in relation to subgroup and electropherotype of genomic RNA.

    abstract::A total of 41 stool rotavirus specimens collected from children with acute diarrhea at four different locations in Akita Prefecture, Japan, during the peak of the winter diarrhea epidemic in 1988 were analyzed by polyacrylamide gel electrophoresis of viral RNA in conjunction with subgrouping assay. We found that a sin...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890280311

    authors: Nakagomi O,Oyamada H,Kuroki S,Kobayashi Y,Ohshima A,Nakagomi T

    更新日期:1989-07-01 00:00:00

  • Early inhibition of nitric oxide production increases HSV-1 intranasal infection.

    abstract::Here, we studied the role of nitric oxide (NO) production during the first steps of the respiratory infection of BALB/c mice with herpes simplex virus type 1 (HSV-1), strain F. Nitric oxide synthase II (NOS-II) mRNA and protein were detected by reverse transcription (RT)-PCR and dot blot, respectively in samples of lu...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20093

    authors: Gamba G,Cavalieri H,Courreges MC,Massouh EJ,Benencia F

    更新日期:2004-06-01 00:00:00

  • Fulminant demyelinating encephalomyelitis associated with productive HHV-6 infection in an immunocompetent adult.

    abstract::Human herpesvirus 6 (HHV-6), the etiologic agent of roseola in young children, has been reported to be detectable in the brain of many neurologically normal adults, although regional localization to plaques of multiple sclerosis has also been demonstrated. Large amounts of this virus were present in multifocal demyeli...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/(sici)1096-9071(199707)52:3<301::aid-jmv11

    authors: Novoa LJ,Nagra RM,Nakawatase T,Edwards-Lee T,Tourtellotte WW,Cornford ME

    更新日期:1997-07-01 00:00:00

  • Frequent detection of hepatitis B virus variants associated with lamivudine resistance in treated South African patients infected chronically with different HBV genotypes.

    abstract::This retrospective study investigated and characterized the YMDD motif of the hepatitis B virus (HBV) reverse transcriptase (RT) gene, in sequential samples of 17 South African patients with chronic hepatitis B infection on lamivudine treatment. The profile of HBV genotypes as well as the genetic variability of pre-co...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21479

    authors: Selabe SG,Song E,Burnett RJ,Mphahlele MJ

    更新日期:2009-06-01 00:00:00

  • Infection with GB virus C in patients with chronic liver disease.

    abstract::Infection with putative non-A to E hepatitis virus, designated GB virus C (GBV-C), was surveyed in 286 patients with chronic liver disease in Japan. RNA of GBV-C was detected, by reverse-transcription polymerase chain reaction with nested primers from the 5'-noncoding region, in 19 patients (6.6%) at a frequency highe...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:

    authors: Sugai Y,Nakayama H,Fukuda M,Sawada N,Tanaka T,Tsuda F,Okamoto H,Miyakawa Y,Mayumi M

    更新日期:1997-03-01 00:00:00

  • Use of a generic polymerase chain reaction assay detecting human T-lymphotropic virus (HTLV) types I, II and divergent simian strains in the evaluation of individuals with indeterminate HTLV serology.

    abstract::In countries with a low prevalence of human T-lymphotropic virus (HTLV) infection, indeterminate HTLV serologies are a major problem in blood bank screening because of the uncertainties about infection in these cases. The recent discovery of two new types of simian T-lymphotropic viruses (STLV), which give an HTLV-ind...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:

    authors: Vandamme AM,Van Laethem K,Liu HF,Van Brussel M,Delaporte E,de Castro Costa CM,Fleischer C,Taylor G,Bertazzoni U,Desmyter J,Goubau P

    更新日期:1997-05-01 00:00:00

  • Molecular and epidemiological study on nosocomial transmission of HCV in hemodialysis patients in Brazil.

    abstract::An epidemiological and molecular study of hepatitis C virus (HCV) infection was carried out in Brazilian hemodialysis centers. A total of 1,095 patients in all 15 hemodialysis centers in the State of Goiás, Brazil, were studied. All patients were interviewed for possible risk factors to HCV infection and serum samples...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20932

    authors: Carneiro MA,Teles SA,Lampe E,Espírito-Santo MP,Gouveia-Oliveira R,Reis NR,Yoshida CF,Martins RM

    更新日期:2007-09-01 00:00:00

  • Typing of human papillomaviruses in condylomata acuminata from Greece.

    abstract::DNA samples from recurrent condylomata acuminata biopsies of Greek males and females were examined for the presence of human papillomavirus (HPV) DNA using high-stringency Southern blot hybridization analysis. Of the twenty-six biopsies, 25 were positive for the HPV 6/11-related DNA sequences, and when further analyze...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890420311

    authors: Labropoulou V,Balamotis A,Tosca A,Rotola A,Mavromara-Nazos P

    更新日期:1994-03-01 00:00:00

  • Phylogenetic characterization of enterovirus 68 strains in patients with respiratory syndromes in Italy.

    abstract::Enterovirus 68 (EV-D68) was associated with mild to severe respiratory infections. In the last 4 years, circulation of different EV-D68 strains has been documented worldwide. In this study, the phylogenetic characterization of nine EV-D68 strains identified in patients in the 2010-2012 period and 12 additional EV-D68 ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.23821

    authors: Piralla A,Girello A,Grignani M,Gozalo-Margüello M,Marchi A,Marseglia G,Baldanti F

    更新日期:2014-09-01 00:00:00

  • Inhibition of hepatitis C virus NS3 function by antisense oligodeoxynucleotides and protease inhibitor.

    abstract::Hepatitis C Virus (HCV) NS3 protease is an attractive target for antiviral agent development because it is required for viral replication. Because a stable cell culture system or small animal model to study HCV replication is not readily available, we constructed an in vitro model allowing the investigation of NS3 tra...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.2089

    authors: Heintges T,Encke J,zu Putlitz J,Wands JR

    更新日期:2001-12-01 00:00:00

  • Floating density of hepatitis C virus particles and response to interferon treatment.

    abstract::Hepatitis C virus (HCV) particles can be classified into two major fractions according to their floating density in serum. However, the genomic heterogeneity of each fraction and the relationship between this viral characteristic and interferon (IFN) response in patients with chronic hepatitis are not known. In this s...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:

    authors: Sakai A,Kaneko S,Matsushita E,Kobayashi K

    更新日期:1998-05-01 00:00:00

  • The role of autophagy in human cytomegalovirus IE2 expression.

    abstract::The purpose of this study was to determine whether autophagy regulates the expression of human cytomegalovirus (HCMV) immediately early two viral protein (IE2). Rapamycin and 3-methyladenine (3-MA) were used to stimulate or suppress autophagy during HCMV infection. UL122 recombinant plasmid was transfected to overexpr...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.26357

    authors: Zhang X,Xi T,Zhang L,Bi Y,Huang Y,Lu Y,Liu X,Fang F

    更新日期:2020-07-25 00:00:00

  • The use of monoclonal antibodies in (reverse) passive haemagglutination tests for herpes simplex virus antigens and antibodies.

    abstract::Three monoclonal antibodies against herpes simplex virus type 2 have been tested for their suitability as reagents in reverse passive haemagglutination. Two of these antibodies with specificity for virus glycoprotein D, when linked to red blood cells, were able to capture antigens without being agglutinated, but addit...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890110405

    authors: Cranage MP,McLean CS,Buckmaster EA,Minson AC,Wildy P,Coombs RR

    更新日期:1983-01-01 00:00:00

  • Prediction of the virological response to etravirine in clinical practice: Comparison of three genotype algorithms.

    abstract::The current Agence Nationale de Recherche sur le SIDA (ANRS)/International AIDS Society (IAS) algorithm predicts resistance to etravirine for viruses harboring >/=3 mutations from a list of 13 reverse transcriptase (RT) mutations. Two weighted algorithms, best correlated with fold changes to etravirine, have been desc...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21461

    authors: Cotte L,Trabaud MA,Tardy JC,Brochier C,Gilibert RP,Miailhes P,Trépo C,André P

    更新日期:2009-04-01 00:00:00

  • Treatment of junin virus-infected guinea pigs with immune serum: development of late neurological disease.

    abstract::Guinea pigs infected with Argentine hemorrhagic fever virus (Junin) were treated with pooled, homologous convalescent sera. Use of 15,000 or 5,000 therapeutic units of immune sera prevented all signs of illness when administered within 24 hr of infection. We could also prevent illness and death in infected guinea pigs...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890200303

    authors: Kenyon RH,Green DE,Eddy GA,Peters CJ

    更新日期:1986-11-01 00:00:00

  • Virological patterns of HCV patients with failure to interferon-free regimens.

    abstract::The study characterized the virological patterns and the resistance-associated substitutions (RASs) in patients with failure to IFN-free regimens enrolled in the real-life setting. All 87 consecutive HCV patients with failed IFN-free regimens, observed at the laboratory of the University of Campania, were enrolled. Al...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.25022

    authors: Starace M,Minichini C,De Pascalis S,Macera M,Occhiello L,Messina V,Sangiovanni V,Adinolfi LE,Claar E,Precone D,Stornaiuolo G,Stanzione M,Ascione T,Caroprese M,Zampino R,Parrilli G,Gentile I,Brancaccio G,Iovinella V,

    更新日期:2018-05-01 00:00:00

  • Viruses in community-acquired pneumonia in children aged less than 3 years old: High rate of viral coinfection.

    abstract::The occurrence of viral coinfections in childhood pneumonia has received little attention, probably because suitable detection methods have been lacking. Between November 2004 and October 2006, the presence of 14 respiratory viruses in children aged less than 3 years old with community-acquired pneumonia were investig...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21271

    authors: Cilla G,Oñate E,Perez-Yarza EG,Montes M,Vicente D,Perez-Trallero E

    更新日期:2008-10-01 00:00:00

  • Non-isotopic detection of hepatitis C virus quasispecies by single strand conformation polymorphism.

    abstract::In patients infected with the hepatitis C virus (HCV), a heterogeneous population of viruses, so-called quasispecies exists in vivo. The hypervariable regions (HVR) within the second envelope gene (HCV-E2) show particularly highly intratypic variability and are considered to be the target of neutralizing antibodies. T...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/(sici)1096-9071(199711)53:3<245::aid-jmv11

    authors: Lee JH,Stripf T,Roth WK,Zeuzem S

    更新日期:1997-11-01 00:00:00

  • Synthesis of human rotavirus polypeptides in cell culture.

    abstract::The replication strategy of a human serotype 1 rotavirus, adapted to rapid growth in CV-1 cells, was investigated. A single cycle growth curve revealed eclipse and latent periods of 3 and 4 hours, respectively. Although the extent of reduction of host cell protein synthesis was directly related to the multiplicity of ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890250113

    authors: Heath RL,Birch CJ

    更新日期:1988-05-01 00:00:00

  • Regulation of p14ARF expression by HPV-18 E6 variants.

    abstract::A common causative agent for uterine cervical cancer is the human papillomavirus type 18 (HPV-18) which has three phylogenic variants: Asian-Amerindian, European, and African. Each variant shows significant molecular differences in the E6 gene. E6 oncoprotein is a negative regulator of tumor suppressor protein p53, he...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.23568

    authors: Vazquez-Vega S,Sanchez-Suarez LP,Andrade-Cruz R,Castellanos-Juarez E,Contreras-Paredes A,Lizano-Soberon M,Garcia-Carranca A,Benitez Bribiesca L

    更新日期:2013-07-01 00:00:00

  • Induction of HIV-specific antibody response and protection against vaginal SHIV transmission by intranasal immunization with inactivated SHIV-capturing nanospheres in macaques.

    abstract::We have previously reported that concanavalin A-immobilized polystyrene nanospheres (Con A-NS) could efficiently capture HIV-1 particles and that intranasal immunization with inactivated HIV-1-capturing nanospheres (HIV-NS) induced vaginal anti-HIV-1 IgA antibody response in mice. In this study, to evaluate the protec...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20100

    authors: Miyake A,Akagi T,Enose Y,Ueno M,Kawamura M,Horiuchi R,Hiraishi K,Adachi M,Serizawa T,Narayan O,Akashi M,Baba M,Hayami M

    更新日期:2004-07-01 00:00:00

  • Repeated HIV-1 resistance genotyping external quality assessments improve virology laboratory performance.

    abstract::The performance of French virology laboratories belonging to the ANRS network has been assessed annually for 3 years. The performance of these laboratories was compared between the years 2002 and 2003. Ten and 7 coded samples were sent to 38 virology laboratories in 2002 and 45 virology laboratories in 2003, respectiv...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20522

    authors: Descamps D,Delaugerre C,Masquelier B,Ruffault A,Marcelin AG,Izopet J,Chaix ML,Calvez V,Brun-Vézinet F,Costagliola D

    更新日期:2006-02-01 00:00:00