Abstract:
:Three monoclonal antibodies against herpes simplex virus type 2 have been tested for their suitability as reagents in reverse passive haemagglutination. Two of these antibodies with specificity for virus glycoprotein D, when linked to red blood cells, were able to capture antigens without being agglutinated, but addition of immune serum subsequently led to agglutination. Haemagglutination using these monoclonal antibody-linked, antigen-captured red cells was readily applicable to testing human sera for antibodies to herpes simplex virus and the titres obtained correlated with those from virus plaque neutralisation tests. The procedure has been termed "Specific Antigen Capture Passive Haemagglutination." A further monoclonal antibody with specificity for the major DNA-binding protein of type 2 herpes virus-infected cells (a nonstructural protein) showed conventional reverse passive haemagglutination when linked to red blood cells and was specific for type 2 herpes simplex virus. The nature and potential uses of these simple reverse passive haemagglutination procedures using monoclonal antibody reagents are discussed.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Cranage MP,McLean CS,Buckmaster EA,Minson AC,Wildy P,Coombs RRdoi
10.1002/jmv.1890110405subject
Has Abstractpub_date
1983-01-01 00:00:00pages
295-306issue
4eissn
0146-6615issn
1096-9071journal_volume
11pub_type
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