Abstract:
:Rotavirus infection is associated both in vivo and in vitro with a series of subcellular pathological alterations leading to cell lysis. It has been suggested that these modifications can play a key role in the pathogenesis of rotavirus-associated diarrheal disease. We describe the effects of SA-11 rotavirus infection in HT-29 cells, a human enterocyte-like cell line. Cytological analyses suggested that the viral-induced cytopathic process, including chromatin clumping, can be referred to as apoptosis, the cell death pathway alternative to necrosis. A time course of the process was performed to investigate whether rotavirus-associated cell death showed specific injury signs. HT-29-infected cells were analyzed by scanning and transmission electron microscopy and features of apoptosis such as blebbing of the plasma membrane, peripheral condensation of chromatin, and fragmentation of the nucleus were observed. Specific changes occurring in cell-substrate adhesion and in some organelles relevant for viral maturation, i.e., rough endoplasmic reticulum, were detected. These findings indicate a role for apoptosis in the rotavirus infection process and its related cytopathology, and also suggested that specific histological alterations such as derangement of enterocytes are associated with the pathogenesis of rotavirus-induced diarrheal disease and could be a direct consequence of viral-triggered apoptosis.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Superti F,Ammendolia MG,Tinari A,Bucci B,Giammarioli AM,Rainaldi G,Rivabene R,Donelli Gdoi
10.1002/(SICI)1096-9071(199612)50:4<325::AID-JMV8>subject
Has Abstractpub_date
1996-12-01 00:00:00pages
325-34issue
4eissn
0146-6615issn
1096-9071pii
10.1002/(SICI)1096-9071(199612)50:4<325::AID-JMV8>journal_volume
50pub_type
杂志文章abstract::Management of patients infected with high-risk HPV (hrHPV) despite normal colposcopy following abnormal cytology remains a clinical challenge. The aim of this study was to evaluate if, in that specific population, initial HPV 16 and HPV 18 viral loads are predictive of infection clearance over a 24-month follow-up. A ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23276
更新日期:2012-06-01 00:00:00
abstract::No data on antiviral response of HBV genotypes E-H are available so far although these HBV genotypes contribute significantly to the global HBV burden. Of 49 patients with HBV genotypes E-H, 23 received interferon (IFN)-alpha, 12 nucleos(t)ide analogues and 14 patients were untreated. HBV genotype was determined by di...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21588
更新日期:2009-10-01 00:00:00
abstract::The general aim was to investigate the burden of respiratory virus illness in a hospital emergency department, during two different epidemic seasons. Consecutive patients attending an emergency department during two study periods (February/March 2009 and 2010) were enrolled using broad inclusion criteria (fever/preced...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.22205
更新日期:2011-11-01 00:00:00
abstract::The plaque reduction neutralization test (PRNT) has been used routinely in serological studies with such arenaviruses as Junin, Machupo, and Parana. However, difficulties have been encountered in using the PRNT for LCM virus, while conflicting views have been expressed about the reliability and efficacy of the test wi...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890220110
更新日期:1987-05-01 00:00:00
abstract::Human papillomavirus (HPV) detection is used for screening of cervical cancer and genotype-specific persistence has shown to be mandatory for dysplasia development. Aim of this study was to evaluate the clinical performance of HPV DNA Array for cervical intraepithelial neoplasia 2+ (CIN2+) lesion detection. HPV DNA A...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25583
更新日期:2020-01-01 00:00:00
abstract::In order to assess the efficacy of interferon-alpha on hepatitis C viral RNA in patients with chronic hepatitis C, we analyzed the levels of HCV RNA in sera and liver tissues of 16 patients pre- and posttreatment using a semiquantitative polymerase chain reaction method. Fifteen of 16 patients were positive for anti-H...
journal_title:Journal of medical virology
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1002/jmv.1890400114
更新日期:1993-05-01 00:00:00
abstract::Human bocavirus (HBoV) is known to be associated with a variety of clinical manifestation including acute gastroenteritis (AGE). Despite their global prevalence, no data is available on the epidemiology of HBoV associated with AGE in South Africa (SA). Between April 2009 and April 2015, 3765 stool specimens were colle...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25634
更新日期:2020-08-01 00:00:00
abstract::Enteroviruses (EVs) often infect humans, presenting as endemic or epidemic infections. In this study, the diversity of infecting EVs was studied among 146 children and 137 adults in a small community in Cameroon. The participants provided 2,458 fecal samples during 1-year monthly collection; 10 or more samples were ob...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23926
更新日期:2014-12-01 00:00:00
abstract::The S RNA genome segment of hantaviruses carried by Arvicolinae and Sigmodontinae rodents encodes the nucleocapsid (N) protein and has an overlapping (+1) open reading frame (ORF) for a putative nonstructural protein (NSs). The aim of this study was to determine whether the ORF is functional. A protein corresponding t...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20948
更新日期:2007-10-01 00:00:00
abstract::To elucidate the mechanism of heterologous antibody responses to herpes simplex virus (HSV) and varicella-zoster virus (VZV) which occur in some patients with HSV or VZV infections, stronger evidence was sought for the existence of cross-reacting antibodies to these viruses, using antibody absorption procedures. Absor...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890090105
更新日期:1982-01-01 00:00:00
abstract::This is an audit of a new technique to improve the colposcopy service. Samples were tested for high risk HPV DNA using Digene Hybrid Capture II. Sixty-four percent of the sampled women under 30 had detectable high risk HPV DNA, decreasing to 44% in 30--39 year olds and to 27% in women over 40. High risk HPV prevalence...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20401
更新日期:2005-08-01 00:00:00
abstract::In real-world settings, not much information is probably available on the treatment efficacy of the combination of glecaprevir and pibrentasvir (G/P) as a salvage therapy in failures of prior direct-acting antiviral (DAA) regimens. Especially, the evolution of NS5A resistance-associated variants (RAVs) and treatment e...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25278
更新日期:2019-01-01 00:00:00
abstract::The effects of tumor necrosis factor-alpha and/or interferon-gamma on the replication of hepatitis B virus were examined using HB611 cells. These cells were derived from human hepatoblastoma cells, Huh6, by integrating hepatitis B virus DNA, and produce hepatitis B virus continuously. Each of the cytokines inhibited h...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890470314
更新日期:1995-11-01 00:00:00
abstract::Recent studies have shown an association between single nucleotide polymorphisms (SNPs) in the interferon lambda-3 (IFNL3 or IL-28B) and IFNL4 genes and treatment response to hepatitis C virus genotype 1 (HCV-1) infection. The importance of these SNPs for HCV genotype 3 (HCV-3), and particularly HCV genotype 6 (HCV-6)...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23957
更新日期:2014-09-01 00:00:00
abstract::Nasal washings were collected from 27 normal adults during 38 naturally acquired colds. The washings were exhaustively tested using tissue cultures, organ cultures and electron microscopy. Washings yielding no identifiable agent were inoculated into human volunteers, and further specimens obtained from the latter were...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890050306
更新日期:1980-01-01 00:00:00
abstract::Seoul virus (SEOV) is the etiologic agent of hemorrhagic fever with renal syndrome. It is carried by brown rats (Rattus norvegicus), a commensal rodent that closely cohabitates with humans in urban environments. SEOV has a worldwide distribution, and in Europe, it has been found in rats in UK, France, Sweden, and Belg...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25390
更新日期:2019-05-01 00:00:00
abstract::Seventeen out of 30 patients with chronic hepatitis type B with hepatitis B e antigen (HBeAg) in serum remained persistently positive for e antigen, while 13 seroconverted to antibody (anti-HBe) when followed over a period of one to five years. Initial levels of serum hepatitis B virus (HBV) markers, such as the hepat...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890100208
更新日期:1982-01-01 00:00:00
abstract::In earlier studies, hepatitis E virus (HEV) particles were detected in the stools of patients with enterically transmitted non-A, non-B (ENANB) hepatitis, and HEV was etiologically associated with this disease. Such particles have not been observed in the liver, however. We describe the pathological findings in the li...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890310310
更新日期:1990-07-01 00:00:00
abstract::Distinct human papillomavirus (HPV) 18 variants are thought to differ in oncogenic potential and geographic distribution. As such, understanding the regional variants of HPV 18 would be of great importance for evolutionary, epidemiological, and biological analysis. In this regard, the sequence variations of E6 gene we...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26283
更新日期:2020-07-09 00:00:00
abstract::During Epstein-Barr virus (EBV) latency, the EBV genome is largely silenced by methylation. This silencing is overturned during the switch to the lytic cycle. A key event is the production of the viral protein Zta which binds to three Zta-response elements (ZRE) from the Rta promoter (Rp), two of which (ZRE2 and ZRE3)...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24524
更新日期:2016-10-01 00:00:00
abstract::Rapid PCR-based influenza tests are increasingly used as point-of-care diagnostics in hospitals and clinics. To our knowledge, no prior studies have described clinical outcomes with implementation of rapid PCR-based influenza tests in hospitalized adult inpatients. Electronic medical records were used to assess differ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24279
更新日期:2015-12-01 00:00:00
abstract::Immune correlates of protection against human cytomegalovirus (HCMV) infection are still debated. This study aimed to investigate which arm of the immune response plays a major role in protection against HCMV infection in kidney transplant recipients (n = 40) and heart transplant recipients (n = 12). Overall, patients...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25225
更新日期:2018-10-01 00:00:00
abstract:BACKGROUND:Patients with thalassemia may also have cardiac abnormalities due to congenital problems, anemia, and increased burden of iron in their myocardium. This study was designed to evaluate the effects of direct acting antiviral (DAA) therapy on the cardiac function of hepatitis C virus (HCV)-infected patients wit...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25314
更新日期:2019-03-01 00:00:00
abstract::Three monoclonal antibodies against herpes simplex virus type 2 have been tested for their suitability as reagents in reverse passive haemagglutination. Two of these antibodies with specificity for virus glycoprotein D, when linked to red blood cells, were able to capture antigens without being agglutinated, but addit...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890110405
更新日期:1983-01-01 00:00:00
abstract::Although there is a low prevalence rate (around 1% of the population) of infection with hepatitis B virus (HBV) and hepatitis C virus (HCV) in Chile, little is known about the diversity and molecular characteristics of the circulating viruses. In the present study, 40 HBV and 57 HCV samples from Santiago City, Chile, ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21607
更新日期:2009-11-01 00:00:00
abstract::Deletions and insertions in the hepatitis B virus (HBV) X region have been associated with severe forms of liver disease, including hepatocellular carcinoma (HCC). However, the molecular epidemiologic features of this virus have been described rarely. Deletions and insertions in the X region were determined by direct ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21938
更新日期:2011-01-01 00:00:00
abstract::A healthy patient presented to Klinikum Altmühlfranken Weißenburg Hospital, Germany, with two morning attacks of painful muscle spasm in the left upper and lower limbs, without altered consciousness. Full examinations, radiological imaging, electroencephalography, lumbar puncture, and autoimmune profile were either no...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26113
更新日期:2020-10-01 00:00:00
abstract::Human Pegivirus (HPgV), formerly GB virus-C/Hepatitis G virus (GBV-C/HGV), collectively known as GBV-C, is widely spread and has been reported to be associated with non-A-E hepatitis. To our knowledge, no previous study was conducted about HPgV in Qatar. Thus, the objectives of this study were as follows: (i) to deter...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24289
更新日期:2015-12-01 00:00:00
abstract::Therapy failure due to drug resistance development is a common phenomenon in HIV-infected patients. However, when the drug pressure leads to the earliest selection of drug-resistant HIV-1 populations is still unclear. In this study, the extent to which selection of the HIV-1 reverse transcriptase M184I/V mutations occ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21363
更新日期:2009-01-01 00:00:00
abstract::Clinical, virological, and histological findings in four HBsAg chronic carriers who cleared HBV markers from both serum and liver following HDV superinfection are described. The patients were long-term HBsAg carriers and all were HBV-DNA/HBeAg positive. Liver biopsy, obtained from three of the patients between 5 and 1...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890310408
更新日期:1990-08-01 00:00:00