Tumor necrosis factor-alpha and interferon-gamma inhibit synergistically viral replication in hepatitis B virus-replicating cells.

Abstract:

:The effects of tumor necrosis factor-alpha and/or interferon-gamma on the replication of hepatitis B virus were examined using HB611 cells. These cells were derived from human hepatoblastoma cells, Huh6, by integrating hepatitis B virus DNA, and produce hepatitis B virus continuously. Each of the cytokines inhibited hepatitis B virus replication in the cells assessed as the amount of episomal hepatitis B virus DNA, without a decrease in cell viability. When the two cytokines were administered together, the inhibitory effect became greater. Incubation of the cells with 1,000 U/ml tumor necrosis factor-alpha decreased HBV DNA replicative intermediates by 55%, and that with 1,000 U/ml interferon-gamma decreased these by 51%. Furthermore, incubation with 1,000 U/ml tumor necrosis factor-alpha and 1,000 U/ml interferon-gamma in combination decreased HBV DNA replicative intermediates by 71%. In contrast, the amount of hepatitis B virus RNA and secretion of hepatitis B e antigen were not apparently reduced by the cytokines, and 2',5'-oligoadenylate synthetase activity was not detected in the supernatant. These results suggest that tumor necrosis factor-alpha and interferon-gamma inhibit hepatitis B virus replication by blocking some step in reverse transcription and that the 2',5'-oligoadenylate synthetase is not involved in the mechanism underlying the inhibition by these two cytokines.

journal_name

J Med Virol

authors

Kawanishi Y,Hayashi N,Katayama K,Ueda K,Takehara T,Miyoshi E,Mita E,Kasahara A,Fusamoto H,Kamada T

doi

10.1002/jmv.1890470314

subject

Has Abstract

pub_date

1995-11-01 00:00:00

pages

272-7

issue

3

eissn

0146-6615

issn

1096-9071

journal_volume

47

pub_type

杂志文章
  • Epidemiology of an outbreak in a maternity unit of infections with an antigenic variant of Echovirus 11.

    abstract::After an 8-day-old child had died with clinical signs of septicemia, 6 other newborns fell ill. Virus was isolated from various sites from all the 6 children (28 isolations). The agent was identified by cross neutralization tests as an antigenic variant of Echovirus 11. The agent could not be isolated from mothers or ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890090202

    authors: Mertens T,Hager H,Eggers HJ

    更新日期:1982-01-01 00:00:00

  • Complement-mediated cytolytic activity associated with cytomegalovirus complement-fixing IgM antibody.

    abstract::Cytomegalovirus-specific complement-fixing IgM (CMV CF-IgM) antibody was detected in 12/84 (14.3 percent) sera containing CMV-specific IgM. Higher titres were obtained by using a purified antigen preparation containing enveloped virus particles and membrane fragments when compared with a crude extract of infected cell...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890240312

    authors: Booth JC,Mohammed HS

    更新日期:1988-03-01 00:00:00

  • Improved prenatal diagnosis of congenital human cytomegalovirus infection by a modified nested polymerase chain reaction.

    abstract::Two major variables may cause false-negative results in prenatal diagnosis of congenital human cytomegalovirus (HCMV) infection: sensitivity of the techniques(s) used; and time elapsed between maternal infection and antenatal testing. Previous results indicated that rapid HCMV isolation from amniotic fluid samples and...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/(sici)1096-9071(199809)56:1<99::aid-jmv16>

    authors: Revello MG,Sarasini A,Zavattoni M,Baldanti F,Gerna G

    更新日期:1998-09-01 00:00:00

  • Respiratory disease caused by a species B2 adenovirus in a military camp in Turkey.

    abstract::In April 2004, two patients were admitted to hospital in Berlin, Germany, with clinical signs of acute respiratory infection after returning from a military exercise in their home country of Turkey. They were admitted to a high security infectious disease unit as epidemiological data pointed to an outbreak of unknown ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20441

    authors: Chmielewicz B,Benzler J,Pauli G,Krause G,Bergmann F,Schweiger B

    更新日期:2005-10-01 00:00:00

  • Antigenic characterisation of influenza B virus with a new microneutralisation assay: comparison to haemagglutination and sequence analysis.

    abstract::Although the haemagglutination inhibition assay is considered the "gold standard" for antigenic characterisation of influenza viruses, some limitations of this technique are well known. A new microneutralisation assay, as a tool for antigenic characterisation of influenza B viruses, has been standardised and its perfo...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20157

    authors: Ansaldi F,Bacilieri S,Amicizia D,Valle L,Banfi F,Durando P,Sticchi L,Gasparini R,Icardi G,Crovari P

    更新日期:2004-09-01 00:00:00

  • Prevalence of abnormal anal cytology in women infected with HIV.

    abstract::Anal cancer is a rare disease. Nevertheless, it may be a reason for concern among groups in which its incidence is increasing: those who engage in anoreceptive intercourse, promiscuous persons, and those with sexually transmitted infections (HPV and HIV). The aim of this study was to evaluate the prevalence of abnorma...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.23346

    authors: Chaves EB,Folgierini H,Capp E,von Eye Corleta H

    更新日期:2012-09-01 00:00:00

  • Pre-existing YMDD mutants in treatment-naïve patients with chronic hepatitis B are not selected during lamivudine therapy.

    abstract::Although the rate at which mutations in the tyrosine-methionine-aspartate-aspartate (YMDD) motif of hepatitis B virus polymerase form is high during prolonged lamivudine (LAM) therapy, these mutations sometimes occur naturally in treatment-naïve patients with chronic hepatitis B. The prevalence of natural YMDD mutants...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.23191

    authors: Lee SH,Kim HS,Byun IS,Jeong SW,Kim SG,Jang JY,Kim YS,Kim BS

    更新日期:2012-02-01 00:00:00

  • A chemiluminescent, magnetic particle-based immunoassay for the detection of hepatitis C virus core antigen in human serum or plasma.

    abstract::Hepatitis C virus (HCV) exposure in blood donors is determined serologically by the detection of anti-HCV antibodies in serum or plasma. However, a "window" period of 30-70 days after exposure exists where specific antibodies to HCV antigens are not detected. The use of nucleic acid testing for the detection of HCV RN...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20716

    authors: Leary TP,Gutierrez RA,Muerhoff AS,Birkenmeyer LG,Desai SM,Dawson GJ

    更新日期:2006-11-01 00:00:00

  • Epidemiological survey of enterovirus infections occurring in Taiwan between 2000 and 2005: analysis of sentinel physician surveillance data.

    abstract::Enterovirus (EV) infections are common. There are more than 60 known serotypes, and each has different epidemiologic or medical importance. Over 700 physicians from 75% of basic administrative units of Taiwan participated in the "Sentinel Physician Surveillance of Infectious Disease" and reported weekly to the Center ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21006

    authors: Tseng FC,Huang HC,Chi CY,Lin TL,Liu CC,Jian JW,Hsu LC,Wu HS,Yang JY,Chang YW,Wang HC,Hsu YW,Su IJ,Wang JR,CDC-Taiwan Virology Reference Laboratories and Sentinel Physician Network.

    更新日期:2007-12-01 00:00:00

  • Two years outcome of women infected with high risk HPV having normal colposcopy following low-grade or equivocal cytological abnormalities: are HPV16 and 18 viral load clinically useful predictive markers?

    abstract::Management of patients infected with high-risk HPV (hrHPV) despite normal colposcopy following abnormal cytology remains a clinical challenge. The aim of this study was to evaluate if, in that specific population, initial HPV 16 and HPV 18 viral loads are predictive of infection clearance over a 24-month follow-up. A ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.23276

    authors: Carcopino X,Henry M,Mancini J,Giusiano S,Boubli L,Olive D,Tamalet C

    更新日期:2012-06-01 00:00:00

  • Scoring system for risk stratification of viral reactivation during prophylactic antiviral treatment in Korean patients with hepatitis B undergoing anticancer chemotherapy: A multicenter study.

    abstract::Prophylactic antiviral therapy is recommended for hepatitis B virus (HBV)-infected patients with malignancies who are undergoing systemic chemotherapy. In the current study, we aimed to develop a risk scoring system to guide the selection of prophylactic antiviral agents. In this retrospective analysis, we included co...

    journal_title:Journal of medical virology

    pub_type: 杂志文章,多中心研究

    doi:10.1002/jmv.25241

    authors: Kim HY,Yoo JJ,Oh S,Yu SJ,Kim YJ,Yoon JH,Kim W,Jung YJ,Kim BH,Kim CM,Park JW,Lee JH

    更新日期:2018-10-01 00:00:00

  • Anal HPV 16 and 18 viral load: A comparison between HIV-negative and -positive MSM and association with persistence.

    abstract::Does anal HPV viral load explain the difference in anal HPV persistence between HIV-negative and -positive men who have sex with men (MSM)? MSM ≥18 years were recruited in Amsterdam, the Netherlands, in 2010-2011. Anal self-swabs were collected every 6 months and genotyped (SPF10 -PCR-DEIA-LIPA25 -system). HPV16 and H...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.24898

    authors: Marra E,King A,van Logchem E,van der Weele P,Mooij SH,Heijman T,Meijer CJLM,Verhagen DWM,van der Sande MAB,Schim van der Loeff MF

    更新日期:2018-01-01 00:00:00

  • Close relationship between immunoglobulin secreting-cells and Epstein-Barr virus reservoir in patients infected with HIV.

    abstract::Individuals infected with HIV have higher circulating Epstein-Barr virus (EBV) DNA load compared to healthy carriers. This study investigated whether level of spontaneous immunoglobulin secreting cells, one of the major hallmarks of HIV infection, is associated with an increase of EBV DNA load in PBMCs and the spontan...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.23762

    authors: Ouedraogo DE,Tuaillon E,Rubbo PA,Bollore K,Foulongne V,Reynes J,Vendrell JP

    更新日期:2014-01-01 00:00:00

  • Hepatitis C virus genotype 4d in Southern Italy: reconstruction of its origin and spread by a phylodynamic analysis.

    abstract::Hepatitis C Virus (HCV) genotype 4 predominates in Middle East and Central Africa countries. Recently, it has become also prevalent in Southern European countries where it is thought to have been introduced through immigration and the movement of intravenous drug users. In Italy, the prevalence of genotype 4 is partic...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.23384

    authors: Ciccozzi M,Equestre M,Costantino A,Marascio N,Quirino A,Lo Presti A,Cella E,Bruni R,Liberto MC,Focà A,Pisani G,Zehender G,Ciccaglione AR

    更新日期:2012-10-01 00:00:00

  • Detection and molecular characterization of noroviruses and sapoviruses in children admitted to hospital with acute gastroenteritis in Vietnam.

    abstract::Noroviruses (NoV) and sapoviruses (SaV) are recognized as important causes of acute gastroenteritis in children worldwide. In this study, the prevalence and genetic variability of NoV and SaV were determined in hospitalized children <5 years of age with acute gastroenteritis in Hanoi, Vietnam. A total of 501 fecal spe...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.23185

    authors: Trang NV,Luan le T,Kim-Anh le T,Hau VT,Nhung le TH,Phasuk P,Setrabutr O,Shirley H,Vinjé J,Anh DD,Mason CJ

    更新日期:2012-02-01 00:00:00

  • Virus reactivations after autologous hematopoietic stem cell transplantation detected by multiplex PCR assay.

    abstract::Several studies have indicated that viral reactivations following allogeneic hematopoietic stem cell transplantation (allo-HSCT) are frequent, but viral reactivations after autologous HSCT (auto-HSCT) have not been investigated in detail. We performed multiplex polymerase chain reaction (PCR) assay to examine multiple...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.24621

    authors: Inazawa N,Hori T,Nojima M,Saito M,Igarashi K,Yamamoto M,Shimizu N,Yoto Y,Tsutsumi H

    更新日期:2017-02-01 00:00:00

  • Genetic relatedness of Cuban HAV wild-type isolates.

    abstract::Knowledge of the nucleotide sequence in the region of the putative VP1/2A junction of the Hepatitis A virus (HAV) genome has enabled differentiation of HAV strains and their classification into seven genotypes, in some of which sub-genotypes A and B can be defined. A 168 base segment encompassing the putative VP1/2A j...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1023

    authors: Diaz BI,Sariol CA,Normann A,Rodríguez L,Flehmig B

    更新日期:2001-06-01 00:00:00

  • High prevalence of GB virus C strains genetically related to strains with Asian origin in Nicaraguan hemophiliacs.

    abstract::The presence of hepatitis GB virus C (GBV-C), also known as hepatitis G virus (HGV), and hepatitis C virus (HCV) were investigated in sera from 45 hemophiliacs from nine locations in Nicaragua using a nested polymerase chain reaction (PCR). Primers used to detect GBV-C and HCV derived from the helicase region and 5'UT...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:

    authors: González-Pérez MA,Norder H,Bergström A,Lopéz E,Visoná KA,Magnius LO

    更新日期:1997-06-01 00:00:00

  • Genotype-specific effects on left ventricular function in parvovirus B19-positive patients with dilated cardiomyopathy.

    abstract::Genotype-specific effects of parvovirus B19 (B19V) infections on left ventricular function in patients with dilated cardiomyopathy (DCM) have not been investigated so far. In this prospective clinical study, the prevalences of B19V genotypes in endomyocardial biopsies from patients presenting with inflammatory heart d...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.22187

    authors: Ruppert V,Meyer T,Balbach A,Richter A,Müller HH,Maisch B,Pankuweit S,German Heart Failure Network.

    更新日期:2011-10-01 00:00:00

  • Experimental infection of nonenveloped DNA virus (TTV) in rhesus monkey.

    abstract::Virus fragments homologous to TTV were detected previously from an enterically transmitted outbreak of non-A-E hepatitis [Luo et al., 1999]. To test the susceptibility of the Rhesus monkey to this virus and to establish its transmission routes, 6 Rhesus monkeys were inoculated, 3 orally and another 3 intravenously. Th...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/(sici)1096-9071(200005)61:1<159::aid-jmv26

    authors: Luo K,Liang W,He H,Yang S,Wang Y,Xiao H,Liu D,Zhang L

    更新日期:2000-05-01 00:00:00

  • Hepatitis C virus genotype 3 and the risk of severe liver disease in a large population of drug users in France.

    abstract::Although risk factors for cirrhosis in chronic hepatitis C virus (HCV) infection have been identified, the role of HCV-genotype 3 remains controversial, and limited data are available in drug users. The aim of the study was to assess risk factors for severe liver disease (cirrhosis/hepatocellular carcinoma) in HCV-inf...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21850

    authors: Larsen C,Bousquet V,Delarocque-Astagneau E,Pioche C,Roudot-Thoraval F,HCV Surveillance Steering Committee.,HCV Surveillance Group.,Desenclos JC

    更新日期:2010-10-01 00:00:00

  • Enterovirus 71 isolated from China is serologically similar to the prototype E71 BrCr strain but differs in the 5'-noncoding region.

    abstract::Enterovirus 71 H (E71 H), an isolate from an adult patient with hand-food-mouth disease (HFMD) in China, was serologically similar to the prototype strain E71 BrCr, which was isolated from a patient with aseptic meningitis. The study further analyzed the similarity of E71 H to E71 BrCr at the 5'-noncoding region (NCR)...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890470209

    authors: Zheng ZM,He PJ,Caueffield D,Neumann M,Specter S,Baker CC,Bankowski MJ

    更新日期:1995-10-01 00:00:00

  • Floating density of hepatitis C virus particles and response to interferon treatment.

    abstract::Hepatitis C virus (HCV) particles can be classified into two major fractions according to their floating density in serum. However, the genomic heterogeneity of each fraction and the relationship between this viral characteristic and interferon (IFN) response in patients with chronic hepatitis are not known. In this s...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:

    authors: Sakai A,Kaneko S,Matsushita E,Kobayashi K

    更新日期:1998-05-01 00:00:00

  • Antibodies to Epstein-Barr virus and cytomegalovirus in relation to CD4 cell number in human immunodeficiency virus 1 infection.

    abstract::Interaction between herpesviruses and human immunodeficiency virus (HIV)1 is postulated in the progression of HIV disease. In order to evaluate the specific antibody responses directed to Epstein-Barr virus (EBV) and cytomegalovirus (CMV) and to provide serological evidence suggesting reactivation of these viruses abl...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890360112

    authors: Quesnel A,Pozzetto B,Touraine F,Moja P,Lucht F,De The G,Touraine JL,Gaudin O,Genin C

    更新日期:1992-01-01 00:00:00

  • Increased prevalence of human herpesvirus type 8 (HHV-8) genome among blood donors from North-Western Argentina.

    abstract::The prevalence of HHV-8 infection varies widely in South American populations, displaying geographical variations in its distribution. The heterogeneous genetic contributions provided by the transatlantic parental populations that modified the Native American genomes may explain this epidemiological observation. Aimin...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.24656

    authors: Hulaniuk ML,Torres O,Bartoli S,Fortuny L,Burgos Pratx L,Nuñez F,Salamone H,Corach D,Trinks J,Caputo M

    更新日期:2017-03-01 00:00:00

  • HULC and 7SL RNA expression levels in patients with Crimean-Congo hemorrhagic fever.

    abstract::Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne disease caused by the Crimean-Congo hemorrhagic fever virus. Long non-coding RNAs (lncRNAs) are generally classified as transcripts longer than 200 nucleotides (nt). The various lncRNAs expressed in infected cells are responsible for regulating the expression of v...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.25264

    authors: Bayyurt B,Arslan S,Engin A,Bakir M

    更新日期:2018-12-01 00:00:00

  • Virus-like particles in the liver of a patient with fulminant hepatitis and antibody to hepatitis E virus.

    abstract::In earlier studies, hepatitis E virus (HEV) particles were detected in the stools of patients with enterically transmitted non-A, non-B (ENANB) hepatitis, and HEV was etiologically associated with this disease. Such particles have not been observed in the liver, however. We describe the pathological findings in the li...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890310310

    authors: Asher LV,Innis BL,Shrestha MP,Ticehurst J,Baze WB

    更新日期:1990-07-01 00:00:00

  • Misidentification of recombinant hepatitis C virus leading to treatment failure with direct acting antivirals.

    abstract::The use of new Direct Acting Antivirals, specific of HCV, has greatly improved the HCV treatment. Most of the DAA are specific of HCV genotypes. Genotyping methods may target different regions of the HCV genome, though only the whole genome sequencing could confirm the correct genotype. The present study describes the...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.25035

    authors: Mourez T,Decroos A,Goria O,Montialoux H,De Oliveira F,Larrat S,Plantier JC,Riachi G

    更新日期:2018-05-01 00:00:00

  • Influence of HLA-B18 on liver fibrosis progression in a cohort of HIV/HCV coinfected individuals.

    abstract::Liver fibrosis is accelerated in human immunodeficiency virus/hepatitis C virus (HIV/HCV) coinfected compared with HCV monoinfected patients, due to multiple cofactors. Recently, HLA-B18 haplotype has been associated with short-term liver disease progression in this population. Our aim was to assess the influence of H...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.25385

    authors: Bavaro DF,Saracino A,Fiordelisi D,Bruno G,Ladisa N,Monno L,Angarano G

    更新日期:2019-05-01 00:00:00

  • Identification of archetype and rearranged forms of BK virus in leukocytes from healthy individuals.

    abstract::BK virus (BKV) establishes a persistent, asymptomatic infection in more than 80% of the human population, and in immunocompromised individuals BKV causes acute urinary tract infections. Two forms of BKV, archetype and rearranged, have been recovered previously from urine samples. The rearranged form is believed to hav...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:

    authors: Chatterjee M,Weyandt TB,Frisque RJ

    更新日期:2000-03-01 00:00:00