Abstract:
:Although the haemagglutination inhibition assay is considered the "gold standard" for antigenic characterisation of influenza viruses, some limitations of this technique are well known. A new microneutralisation assay, as a tool for antigenic characterisation of influenza B viruses, has been standardised and its performance evaluated in comparison with the haemagglutination inhibition test in the light of molecular characterisation of the haemagglutinin. Twelve B viruses belonging to the two lineages and the four sub-lineages discriminated by phylogenetic analysis of HA were tested. The microneutralisation assay clearly distinguishes viruses belonging to different lineages and, in addition, discriminates strains belonging to different sub-lineages that are poorly or not discriminated using the haemagglutination inhibition test. This new microneutralisation assay could provide a useful tool for antigenic characterisation of circulating influenza viruses and contribute, together with the haemagglutination inhibition test and sequence analysis of the haemagglutinin and neuraminidase, in the choice of the strain for use in vaccine composition.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Ansaldi F,Bacilieri S,Amicizia D,Valle L,Banfi F,Durando P,Sticchi L,Gasparini R,Icardi G,Crovari Pdoi
10.1002/jmv.20157keywords:
subject
Has Abstractpub_date
2004-09-01 00:00:00pages
141-6issue
1eissn
0146-6615issn
1096-9071journal_volume
74pub_type
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