Abstract:
:In order to analyse the relationships between enteroviral replication and the myocardial damage at the onset of chronic cardiac infection, 2 mouse strains with different degrees of immunological competence (NMRI nu/nu, DBA/2) were infected by a myocarditic Coxsackie virus B3 (CVB3-M1) variant. At 31 days post-inoculation, plaque-forming assay, polymerase chain reaction (RT-PCR), and immunohistochemistry were carried out for detecting viruses and viral components in the myocardium. The virological findings were related to histopathological changes in the myocardium as well to the dilatation of both cardiac ventricles. Chronic myocardial lesions characterized by large fibrosis areas and interstitial inflammatory infiltrates were detected together with cardiomegalia in 52.6% (10/19) of athymic mice and in 9% (2/22) of euthymic mice. Viral replication foci were located and were found only in myocarditic cells adjacent to myocardial inflammatory lesions by immunostaining myocardial tissue sections with anti-serum to VP1 virus capsid protein. Using PCR followed by microwell capture hybridization assay, a large excess of viral positive strand RNA over negative strand was semiquantified in heart tissue from mice with chronic myocarditis, whereas approximately equal amounts of plus and minus strand RNA were detected in cases of persistent cardiac infection without chronic myocardial injuries. These findings provide evidence of the major role of viral replication in the pathogenesis of chronic murine CVB3-induced cardiomyopathy. The results indicate that the cardiac persistence of enteroviral RNAs can be observed without chronic cardiomyopathy, which could be explained by a defective viral positive RNA replication.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Andréoletti L,Hober D,Becquart P,Belaich S,Copin MC,Lambert V,Wattré Pdoi
10.1002/(sici)1096-9071(199706)52:2<206::aid-jmv15subject
Has Abstractpub_date
1997-06-01 00:00:00pages
206-14issue
2eissn
0146-6615issn
1096-9071pii
10.1002/(SICI)1096-9071(199706)52:2<206::AID-JMV15journal_volume
52pub_type
杂志文章abstract::To characterize the state of dual infection with hepatitis B virus (HBV) and hepatitis C virus (HCV), HBV-DNA and HCV-RNA levels were determined by spot hybridization or polymerase chain reaction in the sera of patients who were positive for both hepatitis B surface antigen and HCV antibody. Among 27 patients who show...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890450222
更新日期:1995-02-01 00:00:00
abstract::In a study in Kenya of corneal lesions occurring in children with measles virus infection, conjunctival lesions of a characteristic appearance were seen concomitant with the rash. The lesions became visible after staining by Lissamine green or Rose Bengal. In order to investigate whether these lesions were virus-induc...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890070210
更新日期:1981-01-01 00:00:00
abstract::The immunogenicity of the Russian cold-adapted (ca) donor stains, A/Leningrad (Len)/134/17/57 and A/Leningrad/134/47/57, and the US strain A/Ann Arbor (AA)/6/60-ca, were compared in BALB/c mice with their respective wild-type parental viruses. Each ca donor strain was less immunogenic than its wild-type parent. The va...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:2001-09-01 00:00:00
abstract::Hepatitis E virus (HEV), the sole member of the genus Hepevirus in the family of Hepeviridae, is the major cause of several outbreaks of waterborne hepatitis in tropical and subtropical countries and of sporadic cases of viral hepatitis in endemic and industrialized countries. Transmission of HEV occurs predominantly ...
journal_title:Journal of medical virology
pub_type: 杂志文章,评审
doi:10.1002/jmv.21116
更新日期:2008-04-01 00:00:00
abstract::A multiplex polymerase chain reaction (PCR) assay that is capable of detecting and typing six serotypes of respiratory adenovirus (Ad) was developed, using multiple sets of type-specific primers. The detection of each different serotype depended on distinguishing different numbers and sizes of amplification products o...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.2174
更新日期:2002-04-01 00:00:00
abstract::A cDNA copy of the HCV229E nucleocapsid protein gene was isolated and characterized. Sequence analysis predicts a nucleocapsid polypeptide of 389 amino acids with a molecular weight (mol. wt.) of 43,450. Single strand RNA probes derived from the cDNA copy hybridize specifically to HCV229E RNA and approximately 50 pg o...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890310216
更新日期:1990-06-01 00:00:00
abstract::Interferon alpha (IFN-alpha) therapy is currently the treatment of choice for chronic hepatitis C (HCV) infection, but it fails to achieve a sustained response in approximately 75% of those treated. The factors which determine whether or not an individual will respond to IFN-alpha are uncertain, although a number of p...
journal_title:Journal of medical virology
pub_type: 临床试验,杂志文章
doi:10.1002/jmv.1890450320
更新日期:1995-03-01 00:00:00
abstract::Hepatitis E is endemic in India. It was recently noted that although all the Indian human hepatitis E virus (HEV) isolates (1976-2001) were placed in genotype I, the swine HEV recovered from western India (2000) belonged to genotype IV. This was in contrast to reports from the United States and Taiwan wherein both hum...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10301
更新日期:2003-03-01 00:00:00
abstract::A line of hepatitis C virus (HCV) transgenic mice was established previously that was mediated by Cre/loxP system using HCV cDNA, including core, E1, E2 and NS2 genes. Intravenous infection of a recombinant adenovirus that expresses Cre DNA recombinase (AxCANCre) induced HCV structural protein expression in the liver ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/1096-9071(200011)62:3<308::aid-jmv2>3.0.co
更新日期:2000-11-01 00:00:00
abstract::For assessing the role of circulating immune complexes (CIC) in chronic hepatitis C, the relative frequency of CIC was determined in 54 patients with chronic hepatitis C, 15 asymptomatic hepatitis C virus (HCV) carriers, and 54 healthy controls. IgM and IgG containing CIC were studied using both C1q and conglutinin (K...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890460104
更新日期:1995-05-01 00:00:00
abstract::Here, we studied the role of nitric oxide (NO) production during the first steps of the respiratory infection of BALB/c mice with herpes simplex virus type 1 (HSV-1), strain F. Nitric oxide synthase II (NOS-II) mRNA and protein were detected by reverse transcription (RT)-PCR and dot blot, respectively in samples of lu...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20093
更新日期:2004-06-01 00:00:00
abstract::Hepatitis C virus (HCV) virions have been shown to be bound to antibodies in patients with chronic HCV infection. The sera from patients infected with genotype 1b HCV contained more antibody-free virions than those from patients with genotype 2a/2b HCV. When compared at the same levels of serum HCV RNA, free virion ti...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:2000-05-01 00:00:00
abstract::It has been generally believed that the Epstein-Barr virus (EBV)-encoded small RNA 1 and 2 (EBER1 and EBER2) genes are conserved as two families that correlated with type 1 (B95-8) and type 2 (AG876 or P3HR-1) EBV strains. EBER polymorphism and its association with EBV-associated disease have not received much attenti...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21714
更新日期:2010-05-01 00:00:00
abstract::Varicella zoster-virus (VZV) is a humanpathogenic alpha-Herpesvirus that causes chickenpox after primary infection. The virus spreads by aerosol or direct contact with infectious vesical fluids, it enters the body via the respiratory tract. In a first viremic stage it replicates in local lymph nodes, followed by a sec...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10323
更新日期:2003-01-01 00:00:00
abstract::Monoclonal antibodies were raised against hepatitis B core antigen (HBcAg) synthesized in Escherichia coli. They identified a single immunodominant determinant on both liver-derived and E. coli-derived HBcAg. Cross-inhibition studies demonstrated that HBsAg-containing human sera which contained antibodies to HBcAg (an...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890190213
更新日期:1986-06-01 00:00:00
abstract::Serum specimens from 1,109 individuals at different ages in Beijing, China, were collected between April 1996 and March 1997 and tested for IgG antibodies against human caliciviruses using enzyme immune assays (EIAs). Baculovirus-expressed recombinant Norwalk virus (rNV) and Mexican virus (rMxV) capsid proteins were u...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(200001)60:1<97::aid-jmv16>
更新日期:2000-01-01 00:00:00
abstract::Human parainfluenza viruses (HPIVs) are an important cause of acute lower respiratory tract infections (ALRTIs). HPIV-4, a newly identified virus, has been associated with severe ALRTIs recently. A total of 771 nasopharyngeal aspirate samples were collected from hospitalized children between March 2010 and February 20...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24580
更新日期:2016-12-01 00:00:00
abstract::A molecular epidemiological study on common diarrheal viruses was conducted in Ho Chi Minh City, Vietnam between October 2002 and September 2003. Fecal samples were collected from 1,010 hospitalized children with acute gastroenteritis. Those samples were screened for groups A, B, and C rotavirus, adenovirus, genogroup...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20857
更新日期:2007-05-01 00:00:00
abstract::JC and BK polyomaviruses (JCV and BKV) infect humans and can cause severe illnesses in immunocompromised patients. Merkel cell polyomavirus (MCPyV) can be found in skin carcinomas. In this study, we assessed the occurrence of serum antibodies against MCPyV, BKV, and JCV polyomaviruses in a healthy population of the Cz...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23841
更新日期:2014-09-01 00:00:00
abstract::Long-term treatment with retrotranscriptase (RT) inhibitors eventually leads to the development of drug resistance. Drug-related mutations occur naturally and these can be found in hepatitis B virus (HBV) carriers who have never received antiviral therapy. HBsAg are overlapped with RT domain, thus nucleot(s)ide analog...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24098
更新日期:2015-06-01 00:00:00
abstract::The prevalence of HHV-8 infection varies widely in South American populations, displaying geographical variations in its distribution. The heterogeneous genetic contributions provided by the transatlantic parental populations that modified the Native American genomes may explain this epidemiological observation. Aimin...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24656
更新日期:2017-03-01 00:00:00
abstract::Within the framework of a comprehensive study of the correlation between enteric diseases and wastewater utilization in agricultural settlements (kibbutzim) the prevalence of several viral antibodies was examined among kibbutz residents and overseas volunteers. The latter were assumed to be a group highly susceptible ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890200211
更新日期:1986-10-01 00:00:00
abstract::Elevated telomerase activity is observed in about 90% of human cancers. This activity correlates strictly with human telomerase reverse transcriptase (hTERT). Previously, it was shown that the Epstein-Barr virus-encoded latent membrane protein 1 (LMP1) induced telomerase activity in nasopharyngeal carcinoma cells. In ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20896
更新日期:2007-08-01 00:00:00
abstract::Coronavirus disease 2019 (COVID-19) has become a serious public health problem worldwide. Here, we stratified COVID-19 patients based on their comorbidities to assess their risk of serious adverse outcomes. We collected 856 hospitalized cases diagnosed with COVID-19 from 17 January to 7 February 2020, in Zhejiang Prov...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26183
更新日期:2020-11-01 00:00:00
abstract::The criterion standard for the diagnosis of occult hepatitis C virus (HCV) infection is detection of HCV-RNA in liver cells. However, because of the invasive nature of liver biopsy, other methods have been studied. The present study aimed to identify subjects with occult HCV-4 infection among healthy sexual partners o...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24074
更新日期:2015-03-01 00:00:00
abstract::Persisting human papillomavirus (HPV) infection is a critical step in cervical carcinogenesis. This study was conducted to determine the type-specific HPV persistence and risk factors for persistence of high-risk HPV infections in a large cohort of Danish women. The study was based on a population-based prospective co...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21750
更新日期:2010-04-01 00:00:00
abstract::Cervical cancer development has been mainly associated with persistent human papillomavirus (HPV) infections. However, HPV infection is unlikely to be sufficient to cause cervical cancer, and the contribution of other sexually transmitted infections (STIs) could be the determining factor for cervical lesion-progressio...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24278
更新日期:2015-12-01 00:00:00
abstract::The plaque reduction neutralization test (PRNT) has been used routinely in serological studies with such arenaviruses as Junin, Machupo, and Parana. However, difficulties have been encountered in using the PRNT for LCM virus, while conflicting views have been expressed about the reliability and efficacy of the test wi...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890220110
更新日期:1987-05-01 00:00:00
abstract::The objective of this study was to investigate variation in human papillomavirus (HPV) type-16 load within histologically defined grades of cervical intraepithelial neoplasia. Two hundred and thirty-seven liquid based cytology samples were collected from women attending colposcopy clinics, DNA was extracted, and prese...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20875
更新日期:2007-09-01 00:00:00
abstract::It is need for development of new means against influenza virus due to the lack of efficacy of available therapeutic strategies. In previous research, 1,8-cineol exert its inhibition of nuclear factor (NF)-κB, the main regulator of cytokine and chemokine production in influenza, and anti-inflammatory activity. These f...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24755
更新日期:2017-07-01 00:00:00