Detection and typing of respiratory adenoviruses in a single-tube multiplex polymerase chain reaction.

Abstract:

:A multiplex polymerase chain reaction (PCR) assay that is capable of detecting and typing six serotypes of respiratory adenovirus (Ad) was developed, using multiple sets of type-specific primers. The detection of each different serotype depended on distinguishing different numbers and sizes of amplification products on agarose gels following PCR. The multiplex PCR was tested with 26 clinical Ad isolates and other respiratory viruses including influenza viruses, parainfluenza viruses, and respiratory syncytial viruses as well as respiratory bacterial pathogens such as Chlamydia pneumoniae, Streptococcus pneumoniae, Haemophilus influenzae, and Mycoplasma pneumoniae. The multiplex PCR for the detection and typing of Ads gave an excellent correlation with the results by conventional typing with type-specific antisera. This assay may serve as a rapid means of confirming Ad with simultaneous serotype identification of the isolates. It will also have relevance as an adjunctive tool to conventional serotyping for diagnostic and epidemiological purposes.

journal_name

J Med Virol

authors

Na BK,Kim JH,Shin GC,Lee JY,Lee JS,Kang C,Kim WJ

doi

10.1002/jmv.2174

keywords:

subject

Has Abstract

pub_date

2002-04-01 00:00:00

pages

512-7

issue

4

eissn

0146-6615

issn

1096-9071

pii

10.1002/jmv.2174

journal_volume

66

pub_type

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