Genetic characterization of human metapneumovirus identified through community and facility-based surveillance of infants in Dhaka, Bangladesh.

Abstract:

BACKGROUND:Acute respiratory infection (ARI) is a leading cause of morbidity and mortality in children in low and middle-income countries. Human metapneumovirus (hMPV) is one of the most common viral etiological agents for ARIs in children. OBJECTIVES:In this study, we explored the genotypic diversity and the epidemiology of hMPV among infants in Dhaka, Bangladesh. STUDY DESIGN:Between December 2014 and August 2016, a total of 3810 mid-turbinate nasal swab samples were collected from infants (0 to 6 months of age) who met clinical ARI criteria, as a part of a prospective ARI cohort study. hMPV was detected using polymerase chain reaction, and genotyped by sequencing and phylogenetic analysis. RESULTS:hMPV was identified in 206 (5.4%) nasal swab specimens. One-tenth of the hMPV-positive swabs (n = 19) were also positive for other respiratory viruses. hMPV activity peaked in January and September in 2015; however, no seasonal pattern of hMPV infection was detected. Phylogenetic analyses of the N and F gene-fragments revealed that the hMPV strains circulating in Dhaka, Bangladesh, belonged to three genotypes: A2b, A2c, and B1. Genotype A (57%) was the predominant hMPV genotype circulating in Bangladesh during the study period. CONCLUSION:This study describes both the epidemiology of hMPV infection and its genotypic strain diversity in Dhaka, Bangladesh.

journal_name

J Med Virol

authors

Rahman MZ,Sumiya M,Sahabuddin M,Pell LG,Gubbay JB,Rahman R,Momtaz F,Azmuda N,Shanta SS,Jahan I,Rahman M,Mahmud AA,Roth DE,Morris SK

doi

10.1002/jmv.25351

subject

Has Abstract

pub_date

2019-04-01 00:00:00

pages

549-554

issue

4

eissn

0146-6615

issn

1096-9071

journal_volume

91

pub_type

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