Clinical Significance of Cellular Immunity Function and Inflammatory Factors Assays in Alveolar Lavage Fluid for Severe COVID-19 Pneumonia.

Abstract:

:In this study, we aimed to investigate the changes of lymphocyte subsets (CD3+ , CD4+ , CD8+ ) and inflammatory factors (interleukin-6 [IL-6], hypersensitive C-reactive protein [HS-CRP], and procalcitonin [PCT]) of alveolar lavage fluid in patients with severe corona virus-2019 (COVID-19) pneumonia and their clinical impact on the assessment of disease severity and prognosis. Twenty-four patients with severe COVID-19 pneumonia were admitted to the intensive care unit (ICU) of the Ezhou Central Hospital from February 1 to March 22, 2020. According to the 28-day prognosis, they were assigned to a death group and a survival group. On the 3rd day of ICU admission, peripheral blood and alveolar lavage fluid were collected for examination of lymphocyte subsets and inflammatory factors by flow cytometry and immunoturbidimetry, respectively. The CD3+ , CD4+ , and CD8+ cell counts in alveolar lavage fluid and serum were significantly higher in the survival group than those of the death group (p < .05). The levels of IL-6, HS-CRP, and PCT in the alveolar lavage fluid and serum of the death group were statistically higher than those of the survival group (p < .05); The CD3+ , CD4+ cell count, and IL-6 level were negatively correlated with Sequential Organ Failure Assessment (SOFA) and Acute Physiology and Chronic Health Evaluation II scores, respectively (p < .05). The CD4+ cell and SOFA score have a regression relationship for the prognosis of COVID-19 severe patients. The CD3+ , CD4+ , CD8+ cells, and IL-6 levels are valuable in determining the prognosis of severe COVID-19 pneumonia and are strongly correlated with the severity of the disease; the CD4+ cell is an independent risk factor affecting the prognosis of COVID-19 pneumonia.

journal_name

J Med Virol

authors

Liao L,Yang GH

doi

10.1002/jmv.26827

subject

Has Abstract

pub_date

2021-01-28 00:00:00

eissn

0146-6615

issn

1096-9071

pub_type

杂志文章
  • Serum thrombospondin-1 is altered in patients with hemorrhagic fever with renal syndrome.

    abstract::Hemorrhagic fever with renal syndrome (HFRS) is a severe acute viral disease with pathological changes of impaired capillary and small vessels and thrombocytopenia. In this study, serum thrombospondin (TSP)-1 concentration in patients with HFRS was determined to explore its possible role in the pathogenesis of HFRS. T...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21270

    authors: Liu Z,Zhao Q,Han Q,Gao M,Zhang N

    更新日期:2008-10-01 00:00:00

  • Human papillomavirus genotypes distribution in cervical samples from Uruguayan women.

    abstract::Persistent infection with high-risk human papillomavirus (HPV) causes cervical preneoplasic lesions and invasive cervical cancer. This study evaluated the prevalence and distribution of HPV genotypes in cervical exfoliated cells from Uruguayan women. Five hundred sixty-eight cervical specimens were examined by PCR usi...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.23479

    authors: Ramas V,Mirazo S,Bonilla S,Mendoza L,Lago O,Basiletti J,González J,Picconi MA,Arbiza J

    更新日期:2013-05-01 00:00:00

  • Ability of different lopinavir genotypic inhibitory quotients to predict 48-week virological response in highly treatment-experienced HIV-infected patients receiving lopinavir/ritonavir.

    abstract::The genotypic inhibitory quotient (GIQ) is the ratio between drug concentration and the number of resistance mutations. However, as different resistance scores can be calculated for the same protease inhibitor, the ability of a GIQ to predict the virological outcome may vary depending on the resistance score used as t...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20736

    authors: Gianotti N,Galli L,Danise A,Hasson H,Boeri E,Lazzarin A,Castagna A

    更新日期:2006-12-01 00:00:00

  • Missed opportunities to use rapid influenza testing and severity assessment to avoid hospital admission: A cohort study from an East London District General Hospital.

    abstract::Annual outbreaks of seasonal influenza cause a substantial health burden. The aim of this study was to compare patient demographic/clinical data in two influenza patient groups presenting to hospital; those requiring O2 or critical care admission and those requiring less intensive treatment. The study was conducted fr...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.26376

    authors: Dominic C,Welch C,Melzer M

    更新日期:2020-07-31 00:00:00

  • Antigenic characterisation of influenza B virus with a new microneutralisation assay: comparison to haemagglutination and sequence analysis.

    abstract::Although the haemagglutination inhibition assay is considered the "gold standard" for antigenic characterisation of influenza viruses, some limitations of this technique are well known. A new microneutralisation assay, as a tool for antigenic characterisation of influenza B viruses, has been standardised and its perfo...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20157

    authors: Ansaldi F,Bacilieri S,Amicizia D,Valle L,Banfi F,Durando P,Sticchi L,Gasparini R,Icardi G,Crovari P

    更新日期:2004-09-01 00:00:00

  • The distribution of hepatitis B virus genotypes in Thailand.

    abstract::Phylogenetic analysis was performed on hepatitis B virus (HBV) strains obtained from 86 hepatitis B surface antigen (HBsAg) positive donors from Thailand originating throughout the country. Based on the S gene, 87.5% of strains were of genotype C while 10.5% were of genotype B, with all genotype B strains obtained fro...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.23363

    authors: Louisirirotchanakul S,Olinger CM,Arunkaewchaemsri P,Poovorawan Y,Kanoksinsombat C,Thongme C,Sa-Nguanmoo P,Krasae S,Theamboonlert A,Oota S,Fongsatitkul L,Puapairoj C,Promwong C,Weber B

    更新日期:2012-10-01 00:00:00

  • High prevalence of hepatitis delta virus infection detectable by enzyme immunoassay among apparently healthy individuals in Mongolia.

    abstract::A previous study revealed a high prevalence of hepatitis B surface antigen (HBsAg) and hepatitis delta virus (HDV) RNA among 249 apparently healthy individuals (mean+/-standard deviation age, 48.4+/-13.9 years; 126 males and 123 females) in Ulaanbaatar, Mongolia. To investigate further the prevalence of HDV infection ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20363

    authors: Inoue J,Takahashi M,Nishizawa T,Narantuya L,Sakuma M,Kagawa Y,Shimosegawa T,Okamoto H

    更新日期:2005-07-01 00:00:00

  • cDNA microarray analysis of HBV transgenic mouse liver identifies genes in lipid biosynthetic and growth control pathways affected by HBV.

    abstract::Hepatitis B virus (HBV) transgenic mice that replicate HBV in the liver generally do not exhibit gross liver pathology, while maintaining a high level (10(7) or greater) of viral titer in the blood. We have used this model to determine the minimum effects of HBV replication in the liver on cellular gene transcription,...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20427

    authors: Hajjou M,Norel R,Carver R,Marion P,Cullen J,Rogler LE,Rogler CE

    更新日期:2005-09-01 00:00:00

  • Characterisation of a series of human immunodeficiency virus isolates derived sequentially from a single patient.

    abstract::Five HIV-1 isolates were obtained sequentially from a single seropositive individual during the later stages of AIDS. Four of these isolates were adapted to grow in a continuous human T-lymphocytic cell line. Comparative biological and biochemical studies of the virus isolates were made using persistently infected cul...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890340207

    authors: Farrar GH,Roff MA,Amin T,Ball J,Parrett AM,Battacharyya U,Booth J,Wansbrough-Jones MH,Greenaway PJ

    更新日期:1991-06-01 00:00:00

  • Improved prenatal diagnosis of congenital human cytomegalovirus infection by a modified nested polymerase chain reaction.

    abstract::Two major variables may cause false-negative results in prenatal diagnosis of congenital human cytomegalovirus (HCMV) infection: sensitivity of the techniques(s) used; and time elapsed between maternal infection and antenatal testing. Previous results indicated that rapid HCMV isolation from amniotic fluid samples and...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/(sici)1096-9071(199809)56:1<99::aid-jmv16>

    authors: Revello MG,Sarasini A,Zavattoni M,Baldanti F,Gerna G

    更新日期:1998-09-01 00:00:00

  • Human parechovirus are emerging pathogens with broad spectrum of clinical syndromes in adults.

    abstract::Parechoviruses are emerging pathogens of humans often affecting the pediatric age group, with a growing line of evidence implicating them as agents of a broad spectrum of clinical syndromes in adults. However, because many clinicians are not familiar with the manifestation of the infections, they are not included in t...

    journal_title:Journal of medical virology

    pub_type: 杂志文章,评审

    doi:10.1002/jmv.26395

    authors: Kabuga AI,Nejati A,Soheili P,Shahmahmoodi S

    更新日期:2020-08-06 00:00:00

  • Human cytomegalovirus envelope glycoprotein B, H, and N polymorphisms among infants of Shanghai area in China.

    abstract::Human cytomegalovirus (HCMV) is the leading cause of congenital infection and an opportunistic pathogen capable of establishing lifelong latency. In the present study, we aimed to investigate the distribution of glycoprotein B, H, and N in infants of Shanghai and correlate the genotype with active and latent HCMV infe...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.26210

    authors: Dong N,Cao L,Su L,Lu L,Dong Z,Xu M,Xu J

    更新日期:2020-06-21 00:00:00

  • New amino acid changes in drug resistance sites and HBsAg in hepatitis B virus genotype H.

    abstract::Long-term treatment with retrotranscriptase (RT) inhibitors eventually leads to the development of drug resistance. Drug-related mutations occur naturally and these can be found in hepatitis B virus (HBV) carriers who have never received antiviral therapy. HBsAg are overlapped with RT domain, thus nucleot(s)ide analog...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.24098

    authors: Fernández-Galindo DA,Sánchez-Ávila F,Bobadilla-Morales L,Gómez-Quiróz P,Bueno-Topete M,Armendáriz-Borunda J,Sánchez-Orozco LV

    更新日期:2015-06-01 00:00:00

  • The impact of high risk human papillomavirus testing in an inner London colposcopy clinic.

    abstract::This is an audit of a new technique to improve the colposcopy service. Samples were tested for high risk HPV DNA using Digene Hybrid Capture II. Sixty-four percent of the sampled women under 30 had detectable high risk HPV DNA, decreasing to 44% in 30--39 year olds and to 27% in women over 40. High risk HPV prevalence...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20401

    authors: Perrons C,Brink N,Jalal H,Watts P,Jelley R

    更新日期:2005-08-01 00:00:00

  • Immune response to guinea pig cytomegalovirus polypeptides and cross reactivity with human cytomegalovirus.

    abstract::The immune response to guinea pig cytomegalovirus (gpCMV) was evaluated by immunoblotting. Preinoculation guinea pig plasma did not react with gpCMV antigen, whereas convalescent plasma reacted to at least 18 gpCMV-specific polypeptides. The initial immune response was primarily directed at polypeptides with MWs of 10...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890320305

    authors: Kacica MA,Harrison CJ,Myers MG,Bernstein DI

    更新日期:1990-11-01 00:00:00

  • Weak correlation between antibody titers and neutralizing activity in sera from SARS-CoV-2 infected subjects.

    abstract::Plenty of serologic tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been developed so far, thus documenting the importance of evaluating the relevant features of the immune response to this viral agent. The performance of these assays is currently under investigation. Amongst them, LIAISON®...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.26605

    authors: Criscuolo E,Diotti RA,Strollo M,Rolla S,Ambrosi A,Locatelli M,Burioni R,Mancini N,Clementi M,Clementi N

    更新日期:2020-10-16 00:00:00

  • Quantitative human papillomavirus 16 and 18 levels in incident infections and cervical lesion development.

    abstract::Human papillomavirus (HPV) RNA levels may be a more sensitive early indicator of predisposition to carcinogenesis than DNA levels. We evaluated whether levels of HPV-16 and HPV-18 DNA and messenger RNA (mRNA) in newly detected infections are associated with cervical lesion development. Female university students were ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21450

    authors: Winer RL,Harris TG,Xi LF,Jansen KU,Hughes JP,Feng Q,Welebob C,Ho J,Lee SK,Carter JJ,Galloway DA,Kiviat NB,Koutsky LA

    更新日期:2009-04-01 00:00:00

  • Active replication of human immunodeficiency virus type 1 by peripheral blood mononuclear cells following coincubation with herpes viruses.

    abstract::Patients with acquired immunodeficiency syndrome (AIDS) commonly suffer from opportunistic infections associated with members of the herpes virus family. To investigate whether certain of these other viruses might have an effect on the ability of the human immunodeficiency virus type 1 (HIV-1) to replicate, we coincub...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890290207

    authors: Tremblay M,Gornitsky M,Wainberg MA

    更新日期:1989-10-01 00:00:00

  • Serologic and viral genome prevalence of HSV, EBV, and HCMV among healthy adults in Wuhan, China.

    abstract::The worldwide infection rate of herpesvirus is high, but the detailed prevalence in China, especially the central area, remains unclear. In the present study, the prevalence of herpes simplex virus (HSV), Epstein-Barr virus (EBV), and human cytomegalovirus (HCMV) was investigated in 303 healthy adults in Wuhan, a repr...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.24989

    authors: Wen L,Qiu Y,Cheng S,Jiang X,Ma YP,Fang W,Wang W,Cui J,Ruan Q,Zhao F,Hu F,Luo MH

    更新日期:2018-03-01 00:00:00

  • Analysis of hepatitis C virus strains circulating in Republic of the Congo.

    abstract::The aim of this study was to assess the seroprevalence, viremia, genotype distribution, and demographic history of hepatitis C virus (HCV) in the Republic of the Congo. Testing was carried out on sera samples collected in 2005 from 807 Bantus belonging to the Kongo, Teke, and Ngala subgroups and 80 Pygmies. Positive H...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21724

    authors: Cantaloube JF,Gallian P,Bokilo A,Jordier F,Biagini P,Attoui H,Chiaroni J,de Micco P

    更新日期:2010-04-01 00:00:00

  • HIV-1 Thai B strain has spread out of former plasma donors into general population through sexual contact in Henan, China.

    abstract::Henan, China is characterized by the outbreak of HIV epidemic of Thai B strain in former plasma donors in 1990s. After the forbidden of paid blood donation, whether Thai B strain will spread out of former plasma donors into sexual transmitted population is unknown. To answer the question, phylogenetic analysis was use...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.24383

    authors: Li L,Sun G,Zhong P,Han J,Li T,Jia D,Liu Y,Sun C,Wang Z,Li H,Wang X,Li J

    更新日期:2016-04-01 00:00:00

  • A variable region in the Crimean-Congo hemorrhagic fever virus L segment distinguishes between strains isolated from different geographic regions.

    abstract::Alignment of Crimean-Congo hemorrhagic fever virus (CCHFV) L genome segment full-length sequences reveals an overall high level of conservation among strains, with greater than 90% of translated amino acid residues strictly conserved. However, a region of marked variability identified previously, corresponding to L po...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20531

    authors: Meissner JD,Seregin SS,Seregin SV,Vyshemirskii OI,Samokhvalov EI,Lvov DK,Netesov SV,Petrov VS

    更新日期:2006-02-01 00:00:00

  • Distribution of BK polyomavirus genotypes in Tunisian renal transplant recipients.

    abstract::BK polyomavirus (BKV) is a ubiquitous virus in humans that remains latent in the urogenital tract after a primary infection during childhood. The virus, which is reactivated frequently and excreted in urine, can cause nephropathy in renal transplant recipients. BKV sequences are classified into four subtypes (I-IV). S...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.22035

    authors: Boukoum H,Nahdi I,Foulongne V,Zallema D,Aloui S,Achour A,Segondy M,Aouni M

    更新日期:2011-04-01 00:00:00

  • Poliovirus and vesicular stomatitis virus replication in the presence of 6-diazo-5-oxo-L-norleucine or 2-deoxy-D-glucose.

    abstract::The effects of 6-diazo-5-oxo-L-norleucine (DON), 2-deoxy-D-glucose (DOG), and tunicamycin (TM) on the replication of poliovirus (PV) and vesicular stomatitis virus (VSV) were examined. During a 48-hr replication period, TM, DON, and DOG inhibit VSV plaque formation in HEp-2 cells by 99.9%, 99.8%, and 99.9% respectivel...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890140210

    authors: Goldstein G,Guskey LE

    更新日期:1984-01-01 00:00:00

  • Eyeglasses in Wonderland of COVID-19.

    abstract::A recent cohort study1 at Suizhou in China identified that the proportion of subjects who wore eyeglasses was lower in hospitalized patients with coronavirus disease 2019 (COVID-19) than in the general population. This article is protected by copyright. All rights reserved. ...

    journal_title:Journal of medical virology

    pub_type: 信件

    doi:10.1002/jmv.26845

    authors: Takagi H

    更新日期:2021-02-02 00:00:00

  • Impact of HIV infection and highly active antiretroviral therapy (HAART) on B cell subpopulations in children.

    abstract::B-cells play an important role in defending children against various infections. In view of scare data, we undertook this prospective cohort study to describe B cell compartment in HIV infected children (<5 years of age) and the effect of HAART on B cell subpopulations. HIV infected children (<5 years) from Pediatric ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.25074

    authors: Singh R,Mukherjee A,Singla M,Vajpayee M,Negi N,Kabra SK,Lodha R,Das BK

    更新日期:2018-07-01 00:00:00

  • Detection of a human rotavirus strain different from types 1 and 2--a new subgroup? Epidemiology of subgroups in a Swedish and an Ethiopian community.

    abstract::A possible new subgroup of human rotavirus was found by crossed immunoelectrophoresis and enzyme-linked immunosorbent assay. The epidemiology of the established subgroups 1 and 2 and this new variant was studied in two different communities. Of 398 rotavirus isolates from Malmö, Sweden, 26.8% were of type 1, 71.7% of ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890120205

    authors: Tufvesson B

    更新日期:1983-01-01 00:00:00

  • Seroprevalence rates of HPyV6, HPyV7, TSPyV, HPyV9, MWPyV and KIPyV polyomaviruses among the healthy blood donors.

    abstract::Human polyomaviruses HPyV6, HPyV7, TSPyV, HPyV9, MWPyV, and KIPyV have been discovered between 2007 and 2012. TSPyV causes a rare skin disease trichodysplasia spinulosa in immunocompromised patients, the role of remaining polyomaviruses in human pathology is not clear. In this study, we assessed the occurrence of seru...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.24440

    authors: Šroller V,Hamšíková E,Ludvíková V,Musil J,Němečková Š,Saláková M

    更新日期:2016-07-01 00:00:00

  • Analysis of clinical and virological factors associated with response to alpha interferon therapy in chronic hepatitis C.

    abstract::Interferon alpha (IFN-alpha) therapy is currently the treatment of choice for chronic hepatitis C (HCV) infection, but it fails to achieve a sustained response in approximately 75% of those treated. The factors which determine whether or not an individual will respond to IFN-alpha are uncertain, although a number of p...

    journal_title:Journal of medical virology

    pub_type: 临床试验,杂志文章

    doi:10.1002/jmv.1890450320

    authors: Garson JA,Brillanti S,Whitby K,Foli M,Deaville R,Masci C,Miglioli M,Barbara L

    更新日期:1995-03-01 00:00:00

  • Antibodies to single stranded DNA: a diagnostic aid in chronic hepatitis B virus infections.

    abstract::Serial determinations of titers of binding antibodies to single stranded DNA were performed over a period of three years in 43 type B hepatitis patients with persisting HBsAg who had either developed chronic hepatitis or were asymptomatic carriers. Patients with histopathological diagnosis of chronic active or chronic...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890040204

    authors: Villarejos VM,Serra J,Visoná KA,Eduarte CE

    更新日期:1979-01-01 00:00:00