Abstract:
:Some international guidelines recommend evaluating the need to confirm positive anti-hepatitis C virus (HCV) antibody screening results by means of a more specific antibody or molecular biology test on the basis of a screening threshold value (such as the sample signal/cut-off ratio) that can predict the positivity of additional antibody testing in at least 95% of cases. The aim of this study was to determine the threshold value of the DiaSorin LIAISON XL chemiluminescence test. Two hundred and twenty-eight routine laboratory samples that were chemiluminescence positive for anti-HCV antibodies but had different signal/cut-off ratios were assayed using immunoblotting, which indicated that 155 (68.0%) were positive, 40 (17.5%) were negative, and 33 (14.5%) were indeterminate. When the samples were divided on the basis of their signal/cut-off ratios, 95.5% of the samples with a ratio of ≥3.5 were positive as against 74.1% of the positive or indeterminate samples with a ratio of <3.5. Statistical analysis using Youden's index and a receiver operating characteristic curve showed that the optimum cut-off value was 3.65. These findings indicate that, when using the LIAISON XL system for anti-HCV antibody screening, a signal/cut-off ratio of ≥3.65 makes further confirmatory tests unnecessary.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
De Paschale M,Manco MT,Arpino O,Ricucci V,Paganini A,Belvisi L,Cagnin D,Cerulli T,Cianflone A,Agrappi C,Mirri P,Clerici Pdoi
10.1002/jmv.24831subject
Has Abstractpub_date
2017-10-01 00:00:00pages
1817-1822issue
10eissn
0146-6615issn
1096-9071journal_volume
89pub_type
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