Human papillomavirus type 11 neutralization in the athymic mouse xenograft system: correlation with virus-like particle IgG concentration.

Abstract:

:Neutralization of virus is likely to be necessary for development of an effective prophylactic vaccine against genital human papillomavirus (HPV) infection. Two New Zealand white rabbits were immunized with purified HPV type 11 (HPV 11) virions in Freund's adjuvant. An enzyme linked immunoassay (ELISA) was used to determine the quantity of IgG which recognized the HPV 11 major capsid protein (L1 protein) virus-like particles (VLPs) in the two anti-HPV 11 sera (serum A and serum B). The concentration of HPV 11 L1 VLP-specific IgG in the A and B sera were determined to be 37 and 90 micrograms per ml, respectively. The A and B sera were used in neutralization experiments in the athymic mouse xenograft system with known quantities of purified HPV 11 virions. The concentration of HPV 11 L1 VLP-specific IgG required to neutralize HPV 11 was determined for each antiserum. This concentration of IgG was approximately 700 to 900 ng per ml. This study demonstrates a positive correlation between the level of HPV 11 L1 VLP-specific IgG in animals immunized with HPV 11 virions and neutralization of HPV 11 in the athymic mouse model. Further studies are needed 1) to determine if sera or genital secretions from other species are neutralizing in the athymic mouse xenograft system, and 2) to determine if the VLP ELISA can be used as a reliable substitute for more cumbersome neutralization assays.

journal_name

J Med Virol

authors

Bryan JT,Jansen KU,Lowe RS,Fife KH,McClowry T,Glass D,Brown DR

doi

10.1002/(sici)1096-9071(199711)53:3<185::aid-jmv1>

subject

Has Abstract

pub_date

1997-11-01 00:00:00

pages

185-8

issue

3

eissn

0146-6615

issn

1096-9071

pii

10.1002/(SICI)1096-9071(199711)53:3<185::AID-JMV1>

journal_volume

53

pub_type

杂志文章
  • Identification of human astrovirus infections among children with acute gastroenteritis in the Southern Part of Vietnam during 2005-2006.

    abstract::A molecular epidemiological study on common diarrheal viruses was conducted in a children's hospital in Ho Chi Minh City between December 2005 and November 2006. Fecal samples were collected from 502 pediatric patients with acute gastroenteritis, and were screened for eight types of viral agents. Human astroviruses (H...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21032

    authors: Nguyen TA,Hoang L,Pham le D,Hoang KT,Mizuguchi M,Okitsu S,Ushijima H

    更新日期:2008-02-01 00:00:00

  • Seroprevalence of hepatitis A virus infection in Central-West of Tunisia.

    abstract::Hepatitis A infections still represent a major global health concern. During the past years, a transition pattern of the hepatitis A epidemiology was noted in many parts of the world. In Tunisia, there is not a recent survey on age-specific hepatitis A virus seroprevalence. This study aimed to investigate the seroprev...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.26563

    authors: Ayouni K,Kharroubi G,Mallekh R,Hammami W,Marouani R,Mhamdi M,Ben Salah A,Triki H,Bettaieb J

    更新日期:2020-09-28 00:00:00

  • Significant impact of non-B HIV-1 variants genetic diversity in Gabon on plasma HIV-1 RNA quantitation.

    abstract::Evaluations of HIV-1 RNA viral load assays are lacking in Central Africa. The main objective of our study was to assess the reliability of HIV-1 RNA results obtained with three different assays for samples collected in Gabon. A total of 137 plasma specimens were assessed for HIV-1 RNA using the Abbott RealTime HIV-1® ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.23770

    authors: Mouinga-Ondémé A,Mabika-Mabika A,Alalade P,Mongo AD,Sica J,Liégeois F,Rouet F

    更新日期:2014-01-01 00:00:00

  • Association of polymorphisms in the vitamin D receptor gene with severity of hand, foot, and mouth disease caused by enterovirus 71.

    abstract::Severe hand, foot, and mouth disease (HFMD) is sometimes associated with critical complications that can cause substantial child mortality. Activity of the vitamin D receptor (VDR) may influence the outcomes of enterovirus 71 (EV71) infection. This case-control study aimed to assess the association of single-nucleotid...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.25349

    authors: Li YP,Deng HL,Xu LH,Wang MQ,Li M,Zhang X,Dang SS

    更新日期:2019-04-01 00:00:00

  • Epidemiology of enterovirus types causing neurological disease in Austria 1999-2007: detection of clusters of echovirus 30 and enterovirus 71 and analysis of prevalent genotypes.

    abstract::Between 1999 and 2007 1,388 stool specimens from patients with acute flaccid paralysis or aseptic meningitis were submitted to the Austrian reference laboratory for poliomyelitis. Samples (201) yielded non-poliovirus enterovirus in culture. One hundred eighty-one viruses were available for typing and 78 isolates which...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21374

    authors: Ortner B,Huang CW,Schmid D,Mutz I,Wewalka G,Allerberger F,Yang JY,Huemer HP

    更新日期:2009-02-01 00:00:00

  • Risk factors and clinical characteristics of patients with nosocomial influenza A infection.

    abstract::Influenza is a public health burden, responsible for more than half a million deaths worldwide each year and explosive outbreaks in-hospital care units. At present, little is known about clinical characteristics and outcomes with nosocomial influenza infection. To assess clinical characteristics and outcome between no...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.25652

    authors: Naudion P,Lepiller Q,Bouiller K

    更新日期:2020-08-01 00:00:00

  • HIV antibodies in whole saliva detected by ELISA and western blot assays.

    abstract::Paired serum and saliva samples were tested by enzyme-linked immunosorbent assay (ELISA) and Western blot (WB) for the presence of human immunodeficiency virus (HIV) antibodies. The study group included 36 individuals known to be HIV seropositive and 14 healthy, seronegative controls. HIV antibodies were detected in a...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890300403

    authors: Holmström P,Syrjänen S,Laine P,Valle SL,Suni J

    更新日期:1990-04-01 00:00:00

  • Development of cell-mediated cytotoxic immunity to respiratory syncytial virus in human infants following naturally acquired infection.

    abstract::With virus-infected autologous and allogenic mononuclear cells as specific targets, the development of cell-mediated cytotoxic reactivity to respiratory syncytial virus (RSV) was studied in peripheral blood lymphocytes (PBL) in groups of infants with acute RSV infection and in other control groups of subjects during a...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890280304

    authors: Chiba Y,Higashidate Y,Suga K,Honjo K,Tsutsumi H,Ogra PL

    更新日期:1989-07-01 00:00:00

  • Liver disease-significant improvement with lamivudine.

    abstract::The natural history of chronic hepatitis B virus (HBV) infection is highly variable, ranging from a benign course to one of shortened life expectancy. Liver histology represents an accurate tool for assessing progressive liver disease, and has been used in five recent Phase III clinical trials of the oral nucleoside a...

    journal_title:Journal of medical virology

    pub_type: 杂志文章,评审

    doi:

    authors: Leung N

    更新日期:2000-07-01 00:00:00

  • Variation in the hepatitis C virus NS5a region in relation to hypervariable region 1 heterogeneity during interferon treatment.

    abstract::The putative interferon sensitivity determining region (ISDR) in the NS5a region of the hepatitis C virus (HCV) was analyzed in 13 interferon alpha (IFN-alpha) treated patients representing genotypes 1a, 1b, and 2b. These patients had previously been followed longitudinally during treatment with respect to viral load ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/(sici)1096-9071(199809)56:1<33::aid-jmv6>3

    authors: Odeberg J,Yun Z,Sönnerborg A,Weiland O,Lundeberg J

    更新日期:1998-09-01 00:00:00

  • Performance of rapid tests for discrimination between HIV-1 and/or HIV-2 infections.

    abstract::Major differences exist between HIV-1 and HIV-2 in terms of epidemiology, pathogenicity, sensitivity to antiretrovirals. Determining the type of HIV infecting a patient is essential for management. The aim of this study was to evaluate the ability of simple/rapid tests to differentiate between HIV-1 and/or HIV-2 infec...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.24282

    authors: Gautheret-Dejean A,Bocobza J,Brunet S,Damond F,Plantier JC,Barin F

    更新日期:2015-12-01 00:00:00

  • Sequence-specific detection method for reverse transcription, loop-mediated isothermal amplification of HIV-1.

    abstract::HIV diagnosis at the point-of-care or in resource-limited settings poses considerable challenges due to time and cost limitations. Currently, nucleic acid-based tests are the only reliable method for diagnosing recent infections during the window period post-infection and pre-seroconversion, but these tests are only s...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21490

    authors: Curtis KA,Rudolph DL,Owen SM

    更新日期:2009-06-01 00:00:00

  • Herpes simplex and human papilloma virus coinfections in oral mucosa of men-A 6-year follow-up study.

    abstract::Herpes simplex virus (HSV) establishes latency in neurons and recurrent infections in oral mucosa. This prospective study analyzes HSV prevalence in oral mucosal brush samples from men with known human papillomavirus (HPV) status. We hypothesized that HSV-1-infection could facilitate HPV persistence as a cofactor. Thi...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.24965

    authors: Mäki J,Paavilainen H,Kero K,Hukkanen V,Syrjänen S

    更新日期:2018-03-01 00:00:00

  • Human T-cell lymphotropic virus antibody prevalence in HIV-1-infected individuals attending a sexual health clinic in South-East London.

    abstract::Human immunodeficiency virus (HIV) and human T-cell lymphotropic virus (HTLV) are both retroviruses with similar routes of transmission. A number of reports suggest variable clinical outcomes in HIV and HTLV co-infected individuals. There is no published information regarding the prevalence of HIV and HTLV co-infectio...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20336

    authors: Cooke FJ,Geretti AM,Zuckerman M

    更新日期:2005-06-01 00:00:00

  • Immune response to guinea pig cytomegalovirus polypeptides and cross reactivity with human cytomegalovirus.

    abstract::The immune response to guinea pig cytomegalovirus (gpCMV) was evaluated by immunoblotting. Preinoculation guinea pig plasma did not react with gpCMV antigen, whereas convalescent plasma reacted to at least 18 gpCMV-specific polypeptides. The initial immune response was primarily directed at polypeptides with MWs of 10...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890320305

    authors: Kacica MA,Harrison CJ,Myers MG,Bernstein DI

    更新日期:1990-11-01 00:00:00

  • Serological diagnosis of parainfluenza virus infections by enzyme immunoassay with special emphasis on purity of viral antigens.

    abstract::Enzyme immunoassay (EIA) for parainfluenza virus type 1, 2, and 3 antibodies was compared with the complement fixation test (CF) as a diagnostic method in 180 patients with respiratory symptoms. The CF test detected rises in parainfluenza virus antibodies in 30 cases, whereas EIA detected 47 rises. Patients with antib...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890140212

    authors: Julkunen I

    更新日期:1984-01-01 00:00:00

  • Molecular characterization of group A rotavirus found in elderly patients in Ireland; predominance of G1P[8], continued presence of G9P[8], and emergence of G2P[4].

    abstract::Rotavirus is a major cause of gastroenteritis in young children worldwide. There have been several recent reports concerning rotavirus isolation from adults, particularly in the elderly, presenting with gastroenteritis. In this study, the authors report on rotavirus outbreaks in five separate elderly care facilities b...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.23416

    authors: Gunn L,Feeney SA,Cashman O,Collins PJ,Coyle PV,O'Shea H

    更新日期:2012-12-01 00:00:00

  • Favorable efficacy of glecaprevir plus pibrentasvir as salvage therapy for HCV failures to prior direct-acting antivirals regimens.

    abstract::In real-world settings, not much information is probably available on the treatment efficacy of the combination of glecaprevir and pibrentasvir (G/P) as a salvage therapy in failures of prior direct-acting antiviral (DAA) regimens. Especially, the evolution of NS5A resistance-associated variants (RAVs) and treatment e...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.25278

    authors: Akuta N,Sezaki H,Suzuki F,Fujiyama S,Kawamura Y,Hosaka T,Kobayashi M,Kobayashi M,Saitoh S,Suzuki Y,Arase Y,Ikeda K,Kumada H

    更新日期:2019-01-01 00:00:00

  • Amino acid changes in the attachment G glycoprotein of human respiratory syncytial viruses (subgroup A) isolated in Italy over several epidemics (1997-2006).

    abstract::The human respiratory syncytial virus (HRSV) is the most important cause of admission to hospital for acute lower respiratory tract infections in infants and young children worldwide. Only few studies have investigated the molecular evolution of HRSV, and none has been conduct ed in Italy. The genetic diversity of the...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21012

    authors: Montieri S,Puzelli S,Ciccozzi M,Calzoletti L,Di Martino A,Milia MG,Rossi A,Piro F,Rezza G,Donatelli I

    更新日期:2007-12-01 00:00:00

  • Growth of human immunodeficiency virus I in cultured cells in the absence of the CD4 antigen.

    abstract::The ability of a variety of epithelial, embryonal, placental, and neuronal cells to express the CD4 antigen and to be infected by human immunodeficiency virus 1 (HIV-1) was examined. Only two (IMR-32 and HeLa-T4) expressed CD4 detectable by indirect immunofluorescence, and both were infectable by HIV-1. Two others, a ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890350308

    authors: Morrison NK,McCarthy K,Hart CA

    更新日期:1991-11-01 00:00:00

  • Coxsackievirus B1 reveals strain specific differences in plasmacytoid dendritic cell mediated immunogenicity.

    abstract::Enterovirus infections are usually mild but can also cause severe illnesses and play a role in chronic diseases, such as cardiomyopathies and type 1 diabetes. Host response to the invading virus can markedly modulate the course of the infection, and this response varies between individuals due to the polymorphism of i...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.23903

    authors: Hämäläinen S,Nurminen N,Ahlfors H,Oikarinen S,Sioofy-Khojine AB,Frisk G,Oberste MS,Lahesmaa R,Pesu M,Hyöty H

    更新日期:2014-08-01 00:00:00

  • Scoring system for risk stratification of viral reactivation during prophylactic antiviral treatment in Korean patients with hepatitis B undergoing anticancer chemotherapy: A multicenter study.

    abstract::Prophylactic antiviral therapy is recommended for hepatitis B virus (HBV)-infected patients with malignancies who are undergoing systemic chemotherapy. In the current study, we aimed to develop a risk scoring system to guide the selection of prophylactic antiviral agents. In this retrospective analysis, we included co...

    journal_title:Journal of medical virology

    pub_type: 杂志文章,多中心研究

    doi:10.1002/jmv.25241

    authors: Kim HY,Yoo JJ,Oh S,Yu SJ,Kim YJ,Yoon JH,Kim W,Jung YJ,Kim BH,Kim CM,Park JW,Lee JH

    更新日期:2018-10-01 00:00:00

  • Improved detection of cytomegalovirus viremia in AIDS patients using shell vial and indirect immunoperoxidase methodologies.

    abstract::One hundred twelve peripheral blood specimens were tested for the presence of cytomegalovirus (CMV) by the tube culture indirect immunoperoxidase (TC-IPA) procedure, the shell vial assay [shell vials were pre- and postinoculation treated with medium containing 2 of 10% fetal bovine serum (FBS) or 100 micrograms% corti...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890380109

    authors: Lipson SM,Kaplan MH,Simon JK,Ciamician Z,Tseng LF

    更新日期:1992-09-01 00:00:00

  • Emergence of clusters of CRF02_AG and B human immunodeficiency viral strains among men having sex with men exhibiting HIV primary infection in southeastern France.

    abstract::The number of new HIV diagnoses is increasing in the western world and transmission clusters have been recently identified among men having sex with men despite Highly Active Antiretroviral Therapy efficacy. The objective of this study was to assess temporal trends, epidemiological, clinical and virological characteri...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.24184

    authors: Tamalet C,Ravaux I,Moreau J,Brégigeon S,Tourres C,Richet H,Abat C,Colson P

    更新日期:2015-08-01 00:00:00

  • Comparison of characteristics between patients with GB virus C/hepatitis G virus (GBV-C/HGV) RNA and those with GBV-C/HGV E2-antibody in patients with hemophilia.

    abstract::We compared characteristics of patients with GB virus C/hepatitis G virus (GBV-C/HGV) RNA to those of patients with GBV-C/HGV E2-antibody. GBV-C/HGV RNA and GBV-C/HGV antibody were assayed in 83 persons with hemophilia using a reverse transcription-polymerase chain reaction and an enzyme-linked immunosorbent assay, re...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/(sici)1096-9071(200001)60:1<34::aid-jmv6>3

    authors: Toyoda H,Takahashi I,Fukuda Y,Hayakawa T,Takamatsu J

    更新日期:2000-01-01 00:00:00

  • Development and application of a one-step real-time RT-PCR using a minor-groove-binding probe for the detection of a novel bunyavirus in clinical specimens.

    abstract::A highly sensitive one-step real-time RT-PCR method using a minor-groove-binding (MGB) probe was developed for detection and quantitation of severe febrile with thrombocytopenia syndrome virus (SFTSV). The assay could discriminate SFTSV infection from other related viral diseases in human with a minimum detection limi...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.23415

    authors: Li Z,Cui L,Zhou M,Qi X,Bao C,Hu J,Shan J,Wu B,Wang S,Guo X,Jiao Y,Tang F,Wang H

    更新日期:2013-02-01 00:00:00

  • DNA of mouse mammary tumor virus-like virus is present in human tumors influenced by hormones.

    abstract::Mouse mammary tumor virus (MMTV) is a hormonally regulated, oncogenic virus of mice. MMTV-like virus DNA has previously been detected in human breast cancers, liver disease, and liver cancers. It is hypothesized that local hormonal effects might be of primary importance in determining MMTV-like virus detection in huma...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21754

    authors: Johal H,Faedo M,Faltas J,Lau A,Mousina R,Cozzi P,Defazio A,Rawlinson WD

    更新日期:2010-05-01 00:00:00

  • Comparative trial of acyclovir and vidarabine in disseminated varicella-zoster virus infections in immunocompromised patients.

    abstract::A comparative assessment of vidarabine and acyclovir in the treatment of varicella and disseminated zoster in immunosuppressed patients was undertaken. Thirty-eight immunosuppressed patients with varicella (N = 18) or disseminated zoster (N = 20) were treated intravenously with 10 mg/kg/day of vidarabine or 30 mg/kg/d...

    journal_title:Journal of medical virology

    pub_type: 临床试验,杂志文章

    doi:10.1002/jmv.1890200205

    authors: Vildé JL,Bricaire F,Leport C,Renaudie M,Brun-Vézinet F

    更新日期:1986-10-01 00:00:00

  • HCV in peripheral blood mononuclear cells are predominantly carried on the surface of cells in HIV/HCV co-infected individuals.

    abstract::HCV replication in extra-hepatic reservoirs has been suggested to occur in many tissues including PBMCs. A recent study showed evidence for compartmentalization and evolution of HCV in PBMCs. However, the cells that support HCV replication in PBMCs have not been identified. In this study we have fractionated the PBMC ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21906

    authors: Natarajan V,Kottilil S,Hazen A,Adelsberger J,Murphy AA,Polis MA,Kovacs JA

    更新日期:2010-12-01 00:00:00

  • Genotype-specific effects on left ventricular function in parvovirus B19-positive patients with dilated cardiomyopathy.

    abstract::Genotype-specific effects of parvovirus B19 (B19V) infections on left ventricular function in patients with dilated cardiomyopathy (DCM) have not been investigated so far. In this prospective clinical study, the prevalences of B19V genotypes in endomyocardial biopsies from patients presenting with inflammatory heart d...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.22187

    authors: Ruppert V,Meyer T,Balbach A,Richter A,Müller HH,Maisch B,Pankuweit S,German Heart Failure Network.

    更新日期:2011-10-01 00:00:00