Abstract:
:Major differences exist between HIV-1 and HIV-2 in terms of epidemiology, pathogenicity, sensitivity to antiretrovirals. Determining the type of HIV infecting a patient is essential for management. The aim of this study was to evaluate the ability of simple/rapid tests to differentiate between HIV-1 and/or HIV-2 infections. We analyzed 116 samples from patients infected with HIV-1 (n = 61), HIV-2 (n = 47), or HIV-1+HIV-2 (n = 8) at the chronic stage of infection. Each sample was tested with SD Bioline HIV-1/2 3.0, ImmunoFlow HIV1-HIV2, ImmunoFlow HIV1-HIV2 (WB), Genie III HIV-1/HIV-2, ImmunoComb HIV1&2 BiSpot. HIV-1, or HIV-2 single infection was identified with a sensitivity ranging from 90% to 100%. The ability to detect dual infection was less sensitive (12.5-100%). SD Bioline HIV-1/2 3.0, ImmunoFlow HIV1-HIV2, and Genie III were unable to detect HIV-1 group O infection in one, one and two cases, respectively. The specificity of detection of HIV-1, HIV-2, or HIV-1+HIV-2 antibodies differed greatly (36-100%). ImmunoComb BiSpot had the highest sensitivity values (99-100% for HIV-1, 98% for HIV-2, and 75-87.5% for dual infection) and specificity values (94-100% for HIV-1, 100% for HIV-2, and 97-100% for dual infection). In conclusion, this study showed that no single rapid test had a perfect sensitivity/specificity ratio, particularly in the case of the double infections.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Gautheret-Dejean A,Bocobza J,Brunet S,Damond F,Plantier JC,Barin Fdoi
10.1002/jmv.24282subject
Has Abstractpub_date
2015-12-01 00:00:00pages
2061-6issue
12eissn
0146-6615issn
1096-9071journal_volume
87pub_type
杂志文章abstract::The ability of hepatitis A virus (HAV) to agglutinate human erythrocytes was used to develop IgM and IgG antibody capture haemadherence tests (MACHAT and GACHAT). Haemadherence was dependent on the pH of the red cell suspension and was best in the pH range 5.4 to 5.8. The tests were applied to serum, urine, and saliva...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890390106
更新日期:1993-01-01 00:00:00
abstract::In order to shorten the time required for the detection of enteroviruses in stool specimens, an 18-h immunoperoxidase test combining low-speed centrifugation and the use of a group specific anti-VP1 monoclonal antibody (5-D8/1, Dako) was developed. This rapid culture assay (RCA) was compared blindly to a conventional ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(199803)54:3<204::aid-jmv11
更新日期:1998-03-01 00:00:00
abstract::Transmission of rotavirus infection was studied in a birth cohort of children based in an urban slum in Vellore and their familial contacts. Contemporaneous samples from index patients and their familial contacts were collected for analysis in three different settings. Firstly, samples were collected from familial con...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21263
更新日期:2008-10-01 00:00:00
abstract::DNA plasmids are potent inducers of long-lasting antigen-specific CTL responses. Little is known about the distribution of antigen-specific CD8+ T cells in the lymphoid tissue and the non-lymphoid tissue after DNA immunization. HBsAg-specific CD8+ T cells in peripheral blood mononuclear cells, spleen, lymph nodes, and...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21039
更新日期:2008-02-01 00:00:00
abstract::Hepatitis E virus sequences were detected by RT-PCR in 14/15 (93%) of untreated sewage samples from Edinburgh, Scotland, UK. Phylogenetic analysis of amplicons at limiting dilution revealed the co-circulation of multiple variants of HEV-3, with a pattern of diversity matching that observed in a local cohort of HEV-inf...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24403
更新日期:2016-05-01 00:00:00
abstract::Genotype 1F was likely localized geographically to China as it has not been reported elsewhere. In this study, whole genome sequences of two rubella 1F virus isolates were completed. Both viruses contained 9,761 nt with a single nucleotide deletion in the intergenic region, compared to the NCBI rubella reference seque...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23936
更新日期:2014-12-01 00:00:00
abstract::The resurgence of Chikungunya virus (CHIKV) in the form of unprecedented and explosive epidemics in India and the Indian Ocean islands after a gap of 32 years is a major public health concern. Currently, there is no specific therapy available to treat CHIKV infection. In the present study, the in vitro prophylactic an...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21663
更新日期:2010-05-01 00:00:00
abstract::High risk types of human papillomaviruses E6/E7 oncogenes and their association with tumor suppressor genes products are the key factors of cervical carcinogenesis. This study proposed them as specific markers for cervical dysplasia screening. The aim of the study is to compare the clinical and prognostic significance...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24199
更新日期:2015-09-01 00:00:00
abstract::The Gaza Strip borders the southern part of Israel and Egypt. There is a remarkable difference in the prevalence of antibodies to hepatitis C virus (HCV) between Israel (0.5%) and Egypt (10%). A few thousand inhabitants cross the borders daily from the Gaza Strip to both countries. The objectives of this study were to...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(199811)56:3<230::aid-jmv9>
更新日期:1998-11-01 00:00:00
abstract::Following widespread outbreaks of oyster-associated gastroenteritis in Australia during 1978 in which Norwalk virus was implicated as the causative agent, collaborative studies were undertaken between laboratories in Australia and the United States to confirm the etiology. Immune electron microscopy (IEM) techniques w...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890060103
更新日期:1980-01-01 00:00:00
abstract::GB Virus C (GBV-C) is a non-pathogenic flavivirus, commonly found in HIV infected patients. Studies suggest a survival benefit of GBV-C viremia in HIV infection. Impact of GBV-C viremia was evaluated on clinical outcome in multidrug-resistant HIV. The OPTIMA study enrolled advanced multidrug-resistant HIV patients wit...
journal_title:Journal of medical virology
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1002/jmv.23845
更新日期:2014-03-01 00:00:00
abstract::Flavivirus infections are a serious healthcare concern in tropical and subtropical countries. Although well-established laboratory tests can provide early diagnosis of acute dengue or Zika infections, access to these tests is limited in developing countries, presenting an urgent need to develop simple, rapid, and robu...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24806
更新日期:2017-09-01 00:00:00
abstract::Because Junin virus (JV) experimental encephalitis of mice and rats is characterized by mild histopathological changes that do not seem to justify per se lethality after intracerebral infection, such a murine model seems adequate to investigate the potential role of inducible nitric oxide synthase (iNOS) as a pathogen...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10254
更新日期:2003-01-01 00:00:00
abstract::A modified centrifugation culture technique and a polymerase chain reaction (PCR) is described for detection of early antigen and IE antigen DNA, respectively, for rapid and sensitive monitoring of active cytomegalovirus (HCMV) infection after organ transplantation. In a preliminary study, 541 clinical specimens (bloo...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890380404
更新日期:1992-12-01 00:00:00
abstract::West Nile virus (WNV) activity has fluctuated in the south-central United States since its introduction. Seasonal outbreaks are common, with three in Oklahoma during 2003, 2007, and 2012. Morbidity and mortality rates vary during each outbreak. Long-term neurologic sequelae in association with West Nile virus disease ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24235
更新日期:2015-10-01 00:00:00
abstract::Genital herpes is a common, distressing infection whose incidence has been underestimated. Recent serological surveys, employing type-specific antibody assays, show a rising prevalence of previous herpes simplex virus (HSV) type 2 infections in post-adolescent populations in developed countries; many of these infectio...
journal_title:Journal of medical virology
pub_type: 杂志文章,评审
doi:10.1002/jmv.1890410508
更新日期:1993-01-01 00:00:00
abstract::Hepatitis A infections still represent a major global health concern. During the past years, a transition pattern of the hepatitis A epidemiology was noted in many parts of the world. In Tunisia, there is not a recent survey on age-specific hepatitis A virus seroprevalence. This study aimed to investigate the seroprev...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26563
更新日期:2020-09-28 00:00:00
abstract::During 2002, several epidemics of aseptic meningitis were attributed to echovirus 13 in Korea. The causative agents of these outbreaks were isolated and identified using rhabdosarcoma cells, HEp-2 and Buffalo green monkey kidney cells, and a neutralization test using monospecific antiserum. Fifty-four echovirus 13 iso...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10543
更新日期:2004-07-01 00:00:00
abstract::Men who have sex with men (MSM) are an important risk group for human immunodeficiency virus (HIV) and at high-risk of herpesvirus infection. However, limited information on epidemiologic patterns of HHV8 and HSV2 among MSM is available in mainland China. A cross-sectional study of 486 participants was performed in Sh...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24718
更新日期:2017-05-01 00:00:00
abstract::Virus variants escaping from host immunity may be implicated in the pathogenesis of hepatitis B virus (HBV) infection. In this cross-sectional study, the association was evaluated of the frequency of amino acid variation within the immunogenic epitopes of surface gene with different disease stages of chronic HBV infec...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10169
更新日期:2002-09-01 00:00:00
abstract::Dengue virus (DV) infection can result in either a mild febrile illness known as dengue fever (DF) or a life-threatening disease called dengue hemorrhagic fever (DHF). DHF is more prevalent in patients undergoing secondary DV infection. This observation has led to the hypothesis that DHF may be the result of immune re...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.2037
更新日期:2001-10-01 00:00:00
abstract::Persistent infection with high-risk human papillomavirus (HPV) causes cervical preneoplasic lesions and invasive cervical cancer. This study evaluated the prevalence and distribution of HPV genotypes in cervical exfoliated cells from Uruguayan women. Five hundred sixty-eight cervical specimens were examined by PCR usi...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23479
更新日期:2013-05-01 00:00:00
abstract::HIV-1 drug resistance mutations (DRMs) and substitutions were assessed after the failure of the first line non-nucleoside reverse transcriptase inhibitors (NNRTIs) + 2 nucleoside/nucleotide reverse transcriptase inhibitors (NRTIs) treatment regimens (efavirenz [EFV] + lamivudine[3TC] + zidovudine [ZDV] vs. EFV + 3TC +...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24361
更新日期:2016-03-01 00:00:00
abstract::Coronavirus disease-2019 (COVID-19) infection and its severity can be explained by the concentration of glycosylated severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) viral particles in the lung epithelium, the concentration of glycosylated angiotensin-converting enzyme receptor 2 (ACE2) in the lung epithel...
journal_title:Journal of medical virology
pub_type: 杂志文章,评审
doi:10.1002/jmv.25887
更新日期:2020-07-01 00:00:00
abstract::A total of 18 rotavirus G9 strains in South Korea were collected during five rotavirus seasons between 2005 and 2010. The relationship between these strains was examined by analyzing the genetic variation of two major structural genes, VP7 and VP4. All the rotavirus isolates were of the G9P[8] genotype. The VP7 phylog...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23436
更新日期:2013-01-01 00:00:00
abstract::Liver fibrosis is accelerated in human immunodeficiency virus/hepatitis C virus (HIV/HCV) coinfected compared with HCV monoinfected patients, due to multiple cofactors. Recently, HLA-B18 haplotype has been associated with short-term liver disease progression in this population. Our aim was to assess the influence of H...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25385
更新日期:2019-05-01 00:00:00
abstract::Hepatitis C virus (HCV), exhibits considerable genetic diversity, but presents a relatively well conserved 5' noncoding region (5' NCR) among all genotypes. In this study, the structural features and translational efficiency of the HCV 5' NCR sequences were analyzed using the programs RNAfold, RNAshapes and RNApdist a...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21507
更新日期:2009-07-01 00:00:00
abstract:OBJECTIVE:The unprecedented demand for testing for the ongoing coronavirus disease-2019 (COVID-19) pandemic caused by SARS-CoV-2 has led to an acute shortage and limited availability of test reagents for which pooling of samples has been recommended in areas with low prevalence. Considering the possibility of dilution ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26618
更新日期:2020-10-21 00:00:00
abstract::It has been reported that a new pattern of mutations, E44D/A and/or V118I, in the reverse transcriptase (RT) gene of HIV-1 confers a moderate level of resistance to lamivudine in the absence of the M184V mutation. The prevalence of this mutational pattern was studied in HIV-1 isolates obtained from 280 patients. These...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.2145
更新日期:2002-03-01 00:00:00
abstract::The purpose of this study was to investigate determinants of endemic hepatitis C virus (HCV) infection within communities in Taiwan. A two-phase study, including a seroprevalence survey and a prevalent case-control study at the first phase, which has been published previously, and a follow-up seroconversion determinat...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:2001-09-01 00:00:00