Abstract:
:Virus variants escaping from host immunity may be implicated in the pathogenesis of hepatitis B virus (HBV) infection. In this cross-sectional study, the association was evaluated of the frequency of amino acid variation within the immunogenic epitopes of surface gene with different disease stages of chronic HBV infection. The surface gene of HBV encompassing the a determinant (amino acids 124-148) and the putative HLA class I restricted cytotoxic T lymphocyte (CTL) epitope (amino acids 28- 51) were amplified and directly sequenced in 33 asymptomatic carriers (Group I), 31 patients with chronic hepatitis (Group II), 22 with cirrhosis (Group III), and 36 with hepatocellular carcinoma (Group IV). The amino acid sequences were compared subsequently with the consensus sequences of HBV serotype adw or adr. The frequency of amino acid variation per site per sequence (FEQ) was analyzed by generalized estimating equation with Poisson model after stratification by clinical and virological features. The FEQ was 1.21% overall, and was highest in Group IV patients and in patients above 50 years of age. In contrast, nine Group IV patients aged below 50 years who were infected with serotype adw had an inversely higher FEQ than those above 50; the age effect among hepatocellular carcinoma patients was significantly different from that among non-cancerous patients (P = 0.04). Variation of amino acid clustered within a determinant and CTL epitope for serotype adw but was distributed at random for serotype adr. Mutation hotspots differed between serotypes adw and adr. The FEQ of HBV surface protein is correlated positively with advancing age and severity of liver disease, and certain variants may contribute to the persistence of HBV infection.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Liu CJ,Kao JH,Shau WY,Chen PJ,Lai MY,Chen DSdoi
10.1002/jmv.10169keywords:
subject
Has Abstractpub_date
2002-09-01 00:00:00pages
50-9issue
1eissn
0146-6615issn
1096-9071journal_volume
68pub_type
杂志文章abstract::The ability of hepatitis A virus (HAV) to agglutinate human erythrocytes was used to develop IgM and IgG antibody capture haemadherence tests (MACHAT and GACHAT). Haemadherence was dependent on the pH of the red cell suspension and was best in the pH range 5.4 to 5.8. The tests were applied to serum, urine, and saliva...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890390106
更新日期:1993-01-01 00:00:00
abstract::To investigate whether the administration of IL-12 is effective against influenza virus infection, mice were intranasally administered IL-12 for three consecutive days and then infected with a non-lethal dose of the influenza virus. The IL-12-treated mice were more resistant to the virus than control mice with respect...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24494
更新日期:2016-09-01 00:00:00
abstract::Hepatitis E virus (HEV), a positive-strand RNA agent, has been associated with enterically transmitted non-A, non-B hepatitis in Asia, Africa, and Mexico. To evaluate the role of HEV in an outbreak of hepatitis in Pakistan, we used immune electron microscopy to detect 1) antibody to HEV, for evidence of infection, and...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890360205
更新日期:1992-02-01 00:00:00
abstract::Human immunodeficiency virus 2 (HIV-2) infection is characterized by a slower disease progression and lower transmission rates. The molecular features that could be assigned as directly involved in this in vivo phenotype remain essentially unknown, and the importance of HIV-2 as a model to understand pathogenicity of ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21619
更新日期:2009-11-01 00:00:00
abstract::During Epstein-Barr virus (EBV) latency, the EBV genome is largely silenced by methylation. This silencing is overturned during the switch to the lytic cycle. A key event is the production of the viral protein Zta which binds to three Zta-response elements (ZRE) from the Rta promoter (Rp), two of which (ZRE2 and ZRE3)...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24524
更新日期:2016-10-01 00:00:00
abstract::The ability of the anti-cancer drug, 9-Nitrocamptothecin (9NC), to inhibit replication of HIV-1 in clinically relevant primary lymphocytic cells was studied. Primary peripheral blood lymphocytes (PBLs) from a non-infected donor were freshly infected with HIV-1 and treated with 9NC by using three different treatment sc...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1042
更新日期:2001-07-01 00:00:00
abstract::Respiratory viruses were identified by the polymerase chain reaction (PCR) in more than 4,200 specimens collected during 2002 and 2003 in Victoria, Australia from patients admitted to hospitals or participating in an influenza surveillance program. Influenza viruses and picornaviruses were important causes of morbidit...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20246
更新日期:2005-01-01 00:00:00
abstract::A healthy patient presented to Klinikum Altmühlfranken Weißenburg Hospital, Germany, with two morning attacks of painful muscle spasm in the left upper and lower limbs, without altered consciousness. Full examinations, radiological imaging, electroencephalography, lumbar puncture, and autoimmune profile were either no...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26113
更新日期:2020-10-01 00:00:00
abstract::A 6-month-old girl with active human cytomegalovirus (HCMV) infection developed Reye's syndrome after vaccination. She suffered from uncommon complications of HCMV infection, including Coombs-negative hemolytic anemia and cardiac injury, but recovered after the appropriate treatment with prompt ganciclovir and symptom...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23475
更新日期:2013-03-01 00:00:00
abstract::Limited amount of information is available in Taiwan on the genetic or antigenic characteristics of influenza A virus prior to the establishment of a Taiwan surveillance network in 2000. Isolates of H1N1 and H3N2 viruses in Taiwan between 1980 and 2006 were studied, and part of the hemagglutinin gene was analyzed due ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21531
更新日期:2009-08-01 00:00:00
abstract::Hepatitis C Virus (HCV) genotype 4 predominates in Middle East and Central Africa countries. Recently, it has become also prevalent in Southern European countries where it is thought to have been introduced through immigration and the movement of intravenous drug users. In Italy, the prevalence of genotype 4 is partic...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23384
更新日期:2012-10-01 00:00:00
abstract::Hepatitis C virus (HCV) has infected approximately 170 million people worldwide. While the seroprevalence of anti-HCV antibody among Iranian blood donors is 0.13%, HCV infection is prevalent in 59-80% of Iranian injecting drug users. One hundred seventy-eight anti-HCV positive patients were referred to the Gastroenter...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23947
更新日期:2014-08-01 00:00:00
abstract::Female renal transplant recipients have an increased incidence of human papillomavirus (HPV) associated lesions, such as cervical dysplasia and neoplasia [Schneider et al., 1983]. In this study we tested the serological responses by enzyme-linked immunoadsorbent assays (ELISA) to 3 different antigenic regions of HPV t...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890400304
更新日期:1993-07-01 00:00:00
abstract::Human cytomegalovirus (HCMV) is the leading cause of congenital infection and an opportunistic pathogen capable of establishing lifelong latency. In the present study, we aimed to investigate the distribution of glycoprotein B, H, and N in infants of Shanghai and correlate the genotype with active and latent HCMV infe...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26210
更新日期:2020-06-21 00:00:00
abstract::To elucidate the mechanism of heterologous antibody responses to herpes simplex virus (HSV) and varicella-zoster virus (VZV) which occur in some patients with HSV or VZV infections, stronger evidence was sought for the existence of cross-reacting antibodies to these viruses, using antibody absorption procedures. Absor...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890090105
更新日期:1982-01-01 00:00:00
abstract::The emergence of variants in the outer envelope proteins of hepatitis B virus (HBV) are found among individuals vaccinated against HBV and asymptomatic carriers of the infection. For example, children in The Gambia vaccinated against hepatitis B may show serological evidence of breakthrough infections, particularly if...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(199908)58:4<346::aid-jmv5>
更新日期:1999-08-01 00:00:00
abstract::Cytomegalovirus (CMV) infection remains a major complication after allogeneic hematopoietic cell transplantation (allo-HCT). We conducted a retrospective study to determine the clinical and economic burden of pre-emptive therapy (PET) for CMV infection in 100 consecutive hospitalized adult CMV positive serostatus allo...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25574
更新日期:2020-01-01 00:00:00
abstract::Genotype-specific effects of parvovirus B19 (B19V) infections on left ventricular function in patients with dilated cardiomyopathy (DCM) have not been investigated so far. In this prospective clinical study, the prevalences of B19V genotypes in endomyocardial biopsies from patients presenting with inflammatory heart d...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.22187
更新日期:2011-10-01 00:00:00
abstract::Human herpesvirus 6 (HHV-6), the etiologic agent of roseola in young children, has been reported to be detectable in the brain of many neurologically normal adults, although regional localization to plaques of multiple sclerosis has also been demonstrated. Large amounts of this virus were present in multifocal demyeli...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(199707)52:3<301::aid-jmv11
更新日期:1997-07-01 00:00:00
abstract::Hepatitis C virus core antigen (HCVcoreAg) may be measured in serum with a sensitive, recently validated assay. Beyond its value as a marker of viral infection, there are little data on its relation with clinical, histological, and virological parameters. In this study, the significance of HCVcoreAg levels was studied...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23672
更新日期:2013-11-01 00:00:00
abstract::Enteroviruses (EVs) are RNA viruses that can cause many clinical syndromes including acute flaccid paralysis (AFP). Within the global polio laboratory network, EVs are categorized either as polioviruses or non-polio enteroviruses (NPEVs). Specific NPEVs have been described in polio-like residual paralytic events in AF...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26804
更新日期:2021-01-17 00:00:00
abstract::The plaque reduction neutralization test (PRNT) has been used routinely in serological studies with such arenaviruses as Junin, Machupo, and Parana. However, difficulties have been encountered in using the PRNT for LCM virus, while conflicting views have been expressed about the reliability and efficacy of the test wi...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890220110
更新日期:1987-05-01 00:00:00
abstract::Hemodialysis patients are at risk for hepatitis C and B virus infections. This study investigated the prevalences and risk factors of HCV and HBV infection and the distribution of HCV genotypes among hemodialysis patients and their spouses. From August to November 2011, a cross-sectional study was conducted on 20 hemo...
journal_title:Journal of medical virology
pub_type: 杂志文章,多中心研究
doi:10.1002/jmv.23486
更新日期:2013-03-01 00:00:00
abstract::Human papilloma virus (HPV) infection is a precursor of cervical cancer. This study aimed to introduce a method to quantify the risk of cervical cancer resulting from infection by different HPV subtypes, to help guide patient treatment. Nucleic acid molecule flow-through hybridization and gene chip technology were use...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24280
更新日期:2015-12-01 00:00:00
abstract::A total of 41 stool rotavirus specimens collected from children with acute diarrhea at four different locations in Akita Prefecture, Japan, during the peak of the winter diarrhea epidemic in 1988 were analyzed by polyacrylamide gel electrophoresis of viral RNA in conjunction with subgrouping assay. We found that a sin...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890280311
更新日期:1989-07-01 00:00:00
abstract::Epstein-Barr virus (EBV) reactivation and cell-mediated immune (CMI) responses were followed in 16 Antarctic expeditioners during winter-over isolation at 2 Australian National Antarctic Research Expedition stations. Delayed-type hypersensitivity (DTH) skin testing was used as an indicator of the CMI response, that wa...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(200006)61:2<235::aid-jmv10
更新日期:2000-06-01 00:00:00
abstract::Recent studies have demonstrated that several cellular factors are involved in entry of hepatitis C virus (HCV) into host cells. Detailed gene expression profiles of these factors in HCV-infected livers have not been reported for humans. Transcriptional levels of LDL receptor (LDLR), CD81, scavenger receptor class B t...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.22042
更新日期:2011-05-01 00:00:00
abstract::In order to shed light on the mode of HTLV-I infection by mother-to-child transmission, we examined sera of school children in a highly endemic town on two separate occasions at a 6-year interval. The carrier rates in ages 15-17, 8.7 and 2.1%, were significantly higher than that in ages 6-8, 1.7 and 0.4%, in studies. ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890200307
更新日期:1986-11-01 00:00:00
abstract::Acute gastroenteritis (AGE) is a common illness in both adults and children worldwide and is caused by several microorganisms including viruses, bacteria, and parasites. Rotavirus (RV), which is the main cause of AGE, can occur as a mixed infection with other viruses. The aim of this study is to assess the molecular e...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26013
更新日期:2020-05-15 00:00:00
abstract::Saffold viruses (SAFV) have been discovered recently and they are classified into Theilovirus species in genus Cardiovirus in the Picornaviridae family. SAFV, especially those belonging to the genotype 2, have been difficult to propagate in laboratory cell lines. This study describes the successful isolation of an eff...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23352
更新日期:2012-09-01 00:00:00