HPV E6/E7 mRNA versus HPV DNA biomarker in cervical cancer screening of a group of Macedonian women.

Abstract:

:High risk types of human papillomaviruses E6/E7 oncogenes and their association with tumor suppressor genes products are the key factors of cervical carcinogenesis. This study proposed them as specific markers for cervical dysplasia screening. The aim of the study is to compare the clinical and prognostic significance of HPV E6/E7 mRNA as an early biomarker versus HPV DNA detection and cytology in triage of woman for cervical cancer. The study group consists of 413 women: 258 NILM, 26 ASC-US, 81 LSIL, 41 HSIL, and 7 unsatisfactory cytology. HPV4AACE screening, real-time multiplex PCR and MY09/11 consensus PCR primers methods were used for the HPV DNA detection. The real-time multiplex nucleic acid sequence-based assay (NucliSENS EasyQ HPV assay) was used for HPV E6/E7 mRNA detection of the five most common high risk HPV types in cervical cancer (16, 18, 31, 33, and 45). The results show that HPV E6/E7 mRNA testing had a higher specificity 50% (95% CI 32-67) and positive predictive value (PPV) 62% (95% CI 46-76) for CIN2+ compared to HPV DNA testing that had specificity of 18% (95% CI 7-37) and PPV 52% (95% CI 39-76) respectively. The higher specificity and PPV of HPV E6/E7 mRNA testing are valuable in predicting insignificant HPV DNA infection among cases with borderline cytological finding. It can help in avoiding aggressive procedures (biopsies and over-referral of transient HPV infections) as well as lowering patient's anxiety and follow up period.

journal_name

J Med Virol

authors

Duvlis S,Popovska-Jankovic K,Arsova ZS,Memeti S,Popeska Z,Plaseska-Karanfilska D

doi

10.1002/jmv.24199

subject

Has Abstract

pub_date

2015-09-01 00:00:00

pages

1578-86

issue

9

eissn

0146-6615

issn

1096-9071

journal_volume

87

pub_type

杂志文章
  • Patterns of serum type 1 and type 2 immune markers in healthy carriers of HTLV-I.

    abstract::Type 1 immunity appears to be diminished in healthy Japanese carriers of human T-lymphotropic virus type I (HTLV-I), but type 2 status remains undetermined. To further examine the subclinical effect of HTLV-I on host immunity, we measured serum antibodies to the Epstein-Barr virus (EBV) in 415 healthy Japanese adults ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20633

    authors: Birmann BM,Mueller NE,Okayama A,Hsieh CC,Tsubouchi H,Harn D,Stuver SO

    更新日期:2006-06-01 00:00:00

  • Shedding of herpes simplex virus type 1 into saliva in patients with orofacial fracture.

    abstract::Shedding of herpes simplex virus type 1 (HSV-1) into saliva was studied in 83 patients with orofacial fractures. Infectious virus was isolated from 14 of 83 patients (16.8%) with no detectable herpetic lesion during hospitalization. Of the 83 patients, 44 (53%) had HSV-1 specific antibody. Virus shedding into saliva w...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890280205

    authors: Kameyama T,Futami M,Nakayoshi N,Sujaku C,Yamamoto S

    更新日期:1989-06-01 00:00:00

  • Fulminant demyelinating encephalomyelitis associated with productive HHV-6 infection in an immunocompetent adult.

    abstract::Human herpesvirus 6 (HHV-6), the etiologic agent of roseola in young children, has been reported to be detectable in the brain of many neurologically normal adults, although regional localization to plaques of multiple sclerosis has also been demonstrated. Large amounts of this virus were present in multifocal demyeli...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/(sici)1096-9071(199707)52:3<301::aid-jmv11

    authors: Novoa LJ,Nagra RM,Nakawatase T,Edwards-Lee T,Tourtellotte WW,Cornford ME

    更新日期:1997-07-01 00:00:00

  • Monoclonal antibody recognizing pre-S(2) epitope of hepatitis B virus: characterization of pre-S(2) epitope and anti-pre-S(2) antibody.

    abstract::A hybrid cell line producing monoclonal antibodies recognizing an epitope encoded by the pre-(S)2 region of hepatitis B virus (HBV) genome was obtained by fusion of mouse myeloma cells with lymphocytes from mice immunized with HBV. The monoclonal antibody Mo-F124 secreted from the hybrid line reacted with the pre-S(2)...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890200204

    authors: Budkowska A,Riottot MM,Dubreuil P,Lazizi Y,Brechot C,Sobczak E,Petit MA,Pillot J

    更新日期:1986-10-01 00:00:00

  • Correlation between hepatitis B virus surface antigen level and alpha-fetoprotein in patients free of hepatocellular carcinoma or severe hepatitis.

    abstract::Alfa-fetoprotein (AFP) is used as a marker of early hepatocarcinogenesis. However, the impact of hepatitis B virus surface antigen (HBsAg) on this relationship in patients with HBV infection is not clear. The present study evaluated the relation between HBsAg and AFP levels at the initial visit in 1,610 untreated HBV ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.23790

    authors: Akuta N,Suzuki F,Kobayashi M,Hara T,Sezaki H,Suzuki Y,Hosaka T,Kobayashi M,Saitoh S,Ikeda K,Kumada H

    更新日期:2014-01-01 00:00:00

  • TTV infection in children born to mothers infected with TTV but not with HBV, HCV, or HIV.

    abstract::The TT virus (TTV) was isolated recently from the serum of a patient with post-transfusion hepatitis. TTV infection is widespread in the general population, and its prevalence increases continuously with age. The pathogenic role of TTV in liver disease remains controversial, and the source of transmission is still unc...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20204

    authors: Komatsu H,Inui A,Sogo T,Kuroda K,Tanaka T,Fujisawa T

    更新日期:2004-11-01 00:00:00

  • Evaluation of Varicella-zoster virus-specific cell-mediated immunity by interferon-γ Enzyme-Linked Immunosorbent Assay in adults ≥50 years of age administered a herpes zoster vaccine.

    abstract::Varicella-zoster virus (VZV)-specific cell-mediated immunity (CMI) is critical for preventing and controlling the onset of herpes zoster (HZ). To assess VZV CMI, an interferon-γ (IFN-γ) enzyme-linked immunosorbent assay (ELISA) was validated by examining the influence of VZV-specific antigen content, incubation time, ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章,随机对照试验

    doi:10.1002/jmv.25391

    authors: Yang P,Chen Z,Zhang J,Li W,Zhu C,Qiu P,Quan Y,Cui X,Yuan L,Jiang C

    更新日期:2019-05-01 00:00:00

  • Scoring system for risk stratification of viral reactivation during prophylactic antiviral treatment in Korean patients with hepatitis B undergoing anticancer chemotherapy: A multicenter study.

    abstract::Prophylactic antiviral therapy is recommended for hepatitis B virus (HBV)-infected patients with malignancies who are undergoing systemic chemotherapy. In the current study, we aimed to develop a risk scoring system to guide the selection of prophylactic antiviral agents. In this retrospective analysis, we included co...

    journal_title:Journal of medical virology

    pub_type: 杂志文章,多中心研究

    doi:10.1002/jmv.25241

    authors: Kim HY,Yoo JJ,Oh S,Yu SJ,Kim YJ,Yoon JH,Kim W,Jung YJ,Kim BH,Kim CM,Park JW,Lee JH

    更新日期:2018-10-01 00:00:00

  • Hepatitis B virus-DNA in the serum of patients followed-up longitudinally with acute and chronic hepatitis B.

    abstract::Sera from 79 patients with acute self-limiting hepatitis, 17 patients with acute hepatitis B evolving into chronic HBsAg carriership, and 43 chronic HBsAg carriers without a history of acute hepatitis were analyzed for presence of hepatitis B virus (HBV)-DNA by a molecular hybridization technique. In acute self-limiti...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890170406

    authors: Moestrup T,Hansson BG,Widell A,Blomberg J,Nordenfelt E

    更新日期:1985-12-01 00:00:00

  • Distribution of BK polyomavirus genotypes in Tunisian renal transplant recipients.

    abstract::BK polyomavirus (BKV) is a ubiquitous virus in humans that remains latent in the urogenital tract after a primary infection during childhood. The virus, which is reactivated frequently and excreted in urine, can cause nephropathy in renal transplant recipients. BKV sequences are classified into four subtypes (I-IV). S...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.22035

    authors: Boukoum H,Nahdi I,Foulongne V,Zallema D,Aloui S,Achour A,Segondy M,Aouni M

    更新日期:2011-04-01 00:00:00

  • Hemagglutination inhibition, single radial hemolysis, and ELISA tests for the detection of IgG and IgM to rubella virus.

    abstract::Hemagglutination inhibition (HI), single radial hemolysis (SRH) and enzyme-linked immuno sorbent assay (ELISA), performed with commercial antigen and reagents are described and were compared in the three distinct situations that require rubella antibody detection. Determination of immunity status was carried out on 15...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/1096-9071(1980)5:4<273::aid-jmv1890050403>

    authors: Champsaur H,Dussaix E,Tournier P

    更新日期:1980-01-01 00:00:00

  • HDV infection in immigrant populations.

    abstract:AIMS:Little data have been published so far on the epidemiological aspects of hepatitis D virus (HDV) infection in immigrant populations and even poorer is the information on the virological, phylogenetic, and clinical aspects of this infection in these populations. This review article, aimed primarily at physicians ca...

    journal_title:Journal of medical virology

    pub_type: 杂志文章,评审

    doi:10.1002/jmv.25570

    authors: Coppola N,Alessio L,Onorato L,Sagnelli C,Sagnelli E,Pisaturo M

    更新日期:2019-12-01 00:00:00

  • Longitudinal analysis of frequency and reactivity of Epstein-Barr virus-specific T lymphocytes and their association with intermittent viral reactivation.

    abstract::Persistent Epstein-Barr virus (EBV) infection is controlled tightly by virus-specific T cells. EBV infection is reactivated intermittently over time, even in apparently healthy carriers. Changes in frequency and reactivity of memory T cells, particularly of CD8(+) origin, have not been assessed in this context. It is ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.22258

    authors: Vogl BA,Fagin U,Nerbas L,Schlenke P,Lamprecht P,Jabs WJ

    更新日期:2012-01-01 00:00:00

  • Concordance of human papillomavirus types detected on the surface and in the tissue of genital lesions in men.

    abstract::Swabbing the surface of a genital lesion to obtain a sample for HPV DNA testing is less invasive than a biopsy, but may not represent HPV types present in the lesion tissue. The objective of this study was to examine the concordance of HPV types detected in swab and biopsy samples from 165 genital lesions from men age...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.23635

    authors: Anic GM,Messina JL,Stoler MH,Rollison DE,Stockwell H,Villa LL,Lazcano-Ponce E,Gage C,Silva RJ,Baggio ML,Salmerón J,Giuliano AR

    更新日期:2013-09-01 00:00:00

  • Treatment of varicella in the immunocompetent adult.

    abstract::Varicella in the immunocompetent adult is an infrequent but potentially serious infection. Previous studies in immunocompetent hosts and normal adults have demonstrated the value of intravenous acyclovir in the treatment of varicella-zoster virus infections. Oral acyclovir has also shown efficacy in both normal adults...

    journal_title:Journal of medical virology

    pub_type: 杂志文章,评审

    doi:10.1002/jmv.1890410517

    authors: Wallace MR,Bowler WA,Oldfield EC 3rd

    更新日期:1993-01-01 00:00:00

  • Treatment with cytotoxic immunosuppression agents increases urinary excretion of JCV in patients with autoimmune disease.

    abstract::Human JC virus is ubiquitous in human populations and is reactivated frequently in immunosuppressed patients. Fifty-one patients with autoimmune disease receiving immunomodulating therapy were evaluated to study the possible relationship between immunosuppression and JCV viruria. Patients were divided into cytotoxic a...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/1096-9071(200012)62:4<505::aid-jmv16>3.0.c

    authors: Wang M,Tsai RT,Ou WC,Lin CK,Tsay GJ,Chang H,Chang D

    更新日期:2000-12-01 00:00:00

  • Failure of influenza vaccination in the aged.

    abstract::A cohort of 127 nursing home residents aged 60-98 years were vaccinated during the winter of 1985-86 with the A-Chile 1/83 (C), A-Philippines 2/82 (P), and B-USSR (B) commercial influenza vaccines. Before vaccination 40%, 23%, and 69% were susceptible to influenza Ac, Ap, and B, respectively [hemagglutinin inhibition ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890250112

    authors: Keren G,Segev S,Morag A,Zakay-Rones Z,Barzilai A,Rubinstein E

    更新日期:1988-05-01 00:00:00

  • The electron microscopical and physical characteristics of small round human fecal viruses: an interim scheme for classification.

    abstract::Many of the small round human fecal viruses implicated in outbreaks of nonbacterial gastroenteritis have been collected together and examined under the electron microscope. Negatively stained preparations without the addition of antibody were used so that the surface morphology of the virus particles remained clearly ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890090403

    authors: Caul EO,Appleton H

    更新日期:1982-01-01 00:00:00

  • Hyperglycemia, hydroxychloroquine, and the COVID-19 pandemic.

    abstract::Coronavirus disease-2019 (COVID-19) infection and its severity can be explained by the concentration of glycosylated severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) viral particles in the lung epithelium, the concentration of glycosylated angiotensin-converting enzyme receptor 2 (ACE2) in the lung epithel...

    journal_title:Journal of medical virology

    pub_type: 杂志文章,评审

    doi:10.1002/jmv.25887

    authors: Brufsky A

    更新日期:2020-07-01 00:00:00

  • Prediction of response to peginterferon-alfa-2b plus ribavirin therapy in Japanese patients infected with hepatitis C virus genotype 1b.

    abstract::Variation at the IL-28B locus was recently reported to be a significant predictive factor of viral response to pegylated-interferon plus ribavirin combination therapy against chronic hepatitis C. Predictive factors for the effect of therapy, including IL-28B polymorphism rs8099917 and viral and clinical factors were i...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.22028

    authors: Hashimoto Y,Ochi H,Abe H,Hayashida Y,Tsuge M,Mitsui F,Hiraga N,Imamura M,Takahashi S,Nelson Hayes C,Ohishi W,Kubo M,Tsunoda T,Kamatani N,Nakamura Y,Chayama K

    更新日期:2011-06-01 00:00:00

  • Molecular surveillance of dengue in Minas Gerais provides insights on dengue virus 1 and 4 circulation in Brazil.

    abstract::Dengue, caused by any of the four types of Dengue virus (DENV) is the most important arbovirus in the world. In this study we performed a molecular surveillance of dengue during the greatest dengue outbreak that took place in Divinópolis, Minas Gerais state, Southeast Brazil, in 2013. Samples from 100 patients with cl...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.24729

    authors: Dutra KR,Drumond BP,de Rezende IM,Nogueira ML,de Oliveira Lopes D,Calzavara Silva CE,Siqueira Ferreira JM,Dos Santos LL

    更新日期:2017-06-01 00:00:00

  • The distribution of hepatitis B virus genotypes in Thailand.

    abstract::Phylogenetic analysis was performed on hepatitis B virus (HBV) strains obtained from 86 hepatitis B surface antigen (HBsAg) positive donors from Thailand originating throughout the country. Based on the S gene, 87.5% of strains were of genotype C while 10.5% were of genotype B, with all genotype B strains obtained fro...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.23363

    authors: Louisirirotchanakul S,Olinger CM,Arunkaewchaemsri P,Poovorawan Y,Kanoksinsombat C,Thongme C,Sa-Nguanmoo P,Krasae S,Theamboonlert A,Oota S,Fongsatitkul L,Puapairoj C,Promwong C,Weber B

    更新日期:2012-10-01 00:00:00

  • Asymptomatic reactivation and shed of infectious varicella zoster virus in astronauts.

    abstract::Varicella zoster virus (VZV) causes varicella (chickenpox), after which virus becomes latent in ganglia along the entire neuraxis. Virus reactivation produces zoster (shingles). Infectious VZV is found in vesicles of patients with zoster and varicella, but virus shed in the absence of disease has not been documented. ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21173

    authors: Cohrs RJ,Mehta SK,Schmid DS,Gilden DH,Pierson DL

    更新日期:2008-06-01 00:00:00

  • Characterization of Epstein-Barr virus (EBV) BZLF1 gene promoter variants and comparison of cellular gene expression profiles in Japanese patients with infectious mononucleosis, chronic active EBV infection, and EBV-associated hemophagocytic lymphohistioc

    abstract::Epstein-Barr virus (EBV) genotypes can be distinguished based on gene sequence differences in EBV nuclear antigens 2, 3A, 3B, and 3C, and the BZLF1 promoter zone (Zp). EBV subtypes and BZLF1 Zp variants were examined in Japanese patients with infectious mononucleosis, chronic active EBV infection, and EBV-associated h...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.23299

    authors: Imajoh M,Hashida Y,Murakami M,Maeda A,Sato T,Fujieda M,Wakiguchi H,Daibata M

    更新日期:2012-06-01 00:00:00

  • Detection of HPV types and neutralizing antibodies in Gansu province, China.

    abstract::A total of 82 samples from patients with cervical cancer (Group 1) and 50 samples from patients with other genital diseases (Group 2) were collected in Gansu, China. All 132 samples were tested for HPV DNA with a typing kit that can detect 21 types of HPV, and also tested for neutralizing antibodies against HPV-16, -1...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21435

    authors: Wu X,Zhang C,Feng S,Liu C,Li Y,Yang Y,Gao J,Li H,Meng S,Li L,Zhang Y,Hu X,Wu X,Lin L,Li X,Wang Y

    更新日期:2009-04-01 00:00:00

  • HIV antibodies in whole saliva detected by ELISA and western blot assays.

    abstract::Paired serum and saliva samples were tested by enzyme-linked immunosorbent assay (ELISA) and Western blot (WB) for the presence of human immunodeficiency virus (HIV) antibodies. The study group included 36 individuals known to be HIV seropositive and 14 healthy, seronegative controls. HIV antibodies were detected in a...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890300403

    authors: Holmström P,Syrjänen S,Laine P,Valle SL,Suni J

    更新日期:1990-04-01 00:00:00

  • Active replication of human immunodeficiency virus type 1 by peripheral blood mononuclear cells following coincubation with herpes viruses.

    abstract::Patients with acquired immunodeficiency syndrome (AIDS) commonly suffer from opportunistic infections associated with members of the herpes virus family. To investigate whether certain of these other viruses might have an effect on the ability of the human immunodeficiency virus type 1 (HIV-1) to replicate, we coincub...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890290207

    authors: Tremblay M,Gornitsky M,Wainberg MA

    更新日期:1989-10-01 00:00:00

  • Valomaciclovir versus valacyclovir for the treatment of acute herpes zoster in immunocompetent adults: a randomized, double-blind, active-controlled trial.

    abstract::Herpes zoster is a common infectious disease that can result in significant acute and chronic morbidity. The safety and efficacy of once-daily oral valomaciclovir (EPB-348) was evaluated for non-inferiority to 3-times daily valacyclovir, an approved therapy. In this study, 373 immunocompetent adults with onset of a he...

    journal_title:Journal of medical virology

    pub_type: 杂志文章,多中心研究,随机对照试验

    doi:10.1002/jmv.23329

    authors: Tyring SK,Plunkett S,Scribner AR,Broker RE,Herrod JN,Handke LT,Wise JM,Martin PA,Valomaciclovir Zoster Study Group.

    更新日期:2012-08-01 00:00:00

  • Lack of augmenting effect of interferon-gamma on dengue virus multiplication in human peripheral blood monocytes.

    abstract::The effect of interferon-gamma (IFN-gamma) on dengue virus multiplication in human peripheral blood monocytes was investigated. Enriched monocytes were treated with IFN-gamma and then infected with dengue virus type 2 either directly or in the presence of optimal infection-enhancing levels of antibodies. Pretreatment ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890450109

    authors: Sittisombut N,Maneekarn N,Kanjanahaluethai A,Kasinrerk W,Viputtikul K,Supawadee J

    更新日期:1995-01-01 00:00:00

  • Improvement of glycemic state among responders to Sofosbuvir-based treatment regimens: Single center experience.

    abstract::Chronic HCV infection has emerged as a complex multifaceted disease with manifestations extending beyond the liver. HCV plays a direct role in glucose metabolism leading to both insulin resistance and type 2 diabetes. To evaluate the changes in the glycemic state following Sofosbuvir-based treatment regimens in diabet...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.24897

    authors: Abdel Alem S,Elsharkawy A,Fouad R,Adel E,Abdellatif Z,Musa S,Nagy A,Hussein MS,Yosry A,Esmat G

    更新日期:2017-12-01 00:00:00