Abstract:
:The effect of interferon-gamma (IFN-gamma) on dengue virus multiplication in human peripheral blood monocytes was investigated. Enriched monocytes were treated with IFN-gamma and then infected with dengue virus type 2 either directly or in the presence of optimal infection-enhancing levels of antibodies. Pretreatment of monocytes from dengue-immune donors with 100 IU/ml of IFN-gamma caused 12- to 97-fold and 13- to 137-fold reduction of virus yields at 24 hr after infection in the absence and presence of an anti-flavivirus monoclonal antibody, respectively. IFN-gamma also diminished virus yields when infection of monocytes from a donor who lacked anti-dengue antibody was enhanced 40-fold. The percentage of infected monocytes in IFN-gamma-pretreated cultures was similarly reduced. Dominance of the antiviral effect of IFN-gamma in monocytes is in contrast to an augmenting effect previously observed in the promonocytic cell line U937.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Sittisombut N,Maneekarn N,Kanjanahaluethai A,Kasinrerk W,Viputtikul K,Supawadee Jdoi
10.1002/jmv.1890450109subject
Has Abstractpub_date
1995-01-01 00:00:00pages
43-9issue
1eissn
0146-6615issn
1096-9071journal_volume
45pub_type
杂志文章abstract::A polymerase chain reaction-restriction (PCR-restriction) endonuclease assay was developed to allow rapid analysis of influenza A H3N2 viruses circulating in England during 1995-1996. Restriction endonuclease digestion with two enzymes of amplicons derived from PCR of the HA1 portion of the influenza haemagglutinin (H...
journal_title:Journal of medical virology
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doi:
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abstract::Subgroup A respiratory syncytial viruses present in respiratory secretions and low passage level cell culture isolates were found to be markedly less susceptible to neutralization with monoclonal antibodies (MAbs) to the F glycoprotein than the cell culture adapted A2 virus strain. Low passage virus isolates collected...
journal_title:Journal of medical virology
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doi:10.1002/jmv.20892
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abstract::Five methods were evaluated for the detection of adenovirus directly from nasopharyngeal aspirates (NPA), including conventional and rapid virus culture, two antigen detection tests, and the polymerase chain reaction (PCR). NPA specimens were obtained from 269 military conscripts suffering from an acute respiratory in...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:1999-09-01 00:00:00
abstract::It remains unclear if China's current HIV antibody testing algorithm misses a substantial number of HIV infected individuals. Of 196 specimens with indeterminate or negative results on HIV western blot (WB) retrospectively examined by HIV-1 nucleic acid test (NAT), 67.57% (75/111) of indeterminate WB samples, and 16.4...
journal_title:Journal of medical virology
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doi:10.1002/jmv.24490
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abstract::Regulatory T cells (T(reg)) provide a balance to immune T cell activation thereby protecting the body from pathogen-induced immunopathology. Several persistent viruses induce T(reg) that subvert protective immune mechanisms and promote viral persistence. Epstein-Barr virus (EBV) generally infects children subclinicall...
journal_title:Journal of medical virology
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doi:10.1002/jmv.21445
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abstract::Serial determinations of titers of binding antibodies to single stranded DNA were performed over a period of three years in 43 type B hepatitis patients with persisting HBsAg who had either developed chronic hepatitis or were asymptomatic carriers. Patients with histopathological diagnosis of chronic active or chronic...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890040204
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abstract::The aim of the study was to evaluate the prevalence and risk factors of HPV in a gynecologic population attending outpatient clinics using two new molecular tests. The Amplicor HPV test and the Linear Array (LA) HPV Genotyping test were used for the detection of HPV DNA in 320 women. Multiple logistic regression was u...
journal_title:Journal of medical virology
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journal_title:Journal of medical virology
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doi:10.1002/jmv.23831
更新日期:2014-03-01 00:00:00
abstract:PROBLEM:Objective studies that use validated questionnaires are needed to evaluate the changes in the sexual functions of women diagnosed with human papillomavirus (HPV) infection. METHOD OF STUDY:The study comprised 80 sexually active women diagnosed with a high-risk HPV infection. These patients were divided into fo...
journal_title:Journal of medical virology
pub_type: 杂志文章
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journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890210209
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journal_title:Journal of medical virology
pub_type: 杂志文章
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更新日期:2013-11-01 00:00:00
abstract::Five temperature-sensitive mutants (tsm9, tsm13, tsm20, tsm22, tsm30) of murine cytomegalovirus have been shown previously not to produce infectious virus in mice. In the present study, the stage at which these mutants are blocked in their replication in vitro was examined by transcriptional analysis of 4 temporally r...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/1096-9071(200010)62:2<127::aid-jmv2>3.0.co
更新日期:2000-10-01 00:00:00
abstract::Mutations of the precore region of hepatitis B virus (HBV) genome have been associated with fulminant and severe chronic hepatitis. However uncertainty remains about the clinical significance and transmissibility of these mutant strains. A point mutation assay (PMA) was developed to identify qualitatively and quantita...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890440105
更新日期:1994-09-01 00:00:00
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journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890350308
更新日期:1991-11-01 00:00:00
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journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23196
更新日期:2012-02-01 00:00:00
abstract::Human bocavirus (HBoV) was first recognized in respiratory samples in 2005. The clinical importance of HBoV infection remains unclear. This report describes the clinical features and molecular phylogeny of HBoV isolates in children with acute respiratory infections. Nasopharyngeal aspirates were obtained from 1,528 ch...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23880
更新日期:2014-12-01 00:00:00
abstract::After an 8-day-old child had died with clinical signs of septicemia, 6 other newborns fell ill. Virus was isolated from various sites from all the 6 children (28 isolations). The agent was identified by cross neutralization tests as an antigenic variant of Echovirus 11. The agent could not be isolated from mothers or ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890090202
更新日期:1982-01-01 00:00:00
abstract::Chikungunya fever is a viral disease transmitted to humans by the bite of infected mosquitoes. The disease is characterized by fever, headache, rash, severe joint, and muscle pain. To evaluate the disease burden in the population and the effectiveness of public health measures, periodic seroprevalence surveys are esse...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25581
更新日期:2020-01-01 00:00:00
abstract::Avidity maturation of IgG antibody responses directed against the structural proteins of rubella virus (E1, E2, and C) as well as whole rubella virus (RV) was assessed at sequential time intervals in 7 individuals following serologically confirmed wild rubella infection. Individual structural proteins were purified fr...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890360310
更新日期:1992-03-01 00:00:00
abstract::West Nile virus (WNV) activity has fluctuated in the south-central United States since its introduction. Seasonal outbreaks are common, with three in Oklahoma during 2003, 2007, and 2012. Morbidity and mortality rates vary during each outbreak. Long-term neurologic sequelae in association with West Nile virus disease ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24235
更新日期:2015-10-01 00:00:00
abstract::Hepatitis E is endemic in India. It was recently noted that although all the Indian human hepatitis E virus (HEV) isolates (1976-2001) were placed in genotype I, the swine HEV recovered from western India (2000) belonged to genotype IV. This was in contrast to reports from the United States and Taiwan wherein both hum...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10301
更新日期:2003-03-01 00:00:00
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journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20668
更新日期:2006-08-01 00:00:00
abstract::A solid-phase radioimmunoassay (RIA) for the detection of hepatitis B e antigen (HBeAg) and antibody (anti-HBe) was developed. The RIA was approximately 1,000-fold more sensitive than rheophoresis for HBeAg, and approximately 6,000-fold more sensitive than rheophoresis for anti-HBe. Generally, less than one-fifth of h...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890020202
更新日期:1978-01-01 00:00:00
abstract::In eight patients with biopsy-confirmed chronic active hepatitis (CAH), hepatitis B e antigen (HBeAg) levels and DNA polymerase (DNA-P) activity were assayed three times a week for six weeks and once a week for another six weeks. HBeAg levels were rather constant, whereas DNA-P activity fluctuated. No correlation was ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/1096-9071(1980)5:4<295::aid-jmv1890050405>
更新日期:1980-01-01 00:00:00
abstract::Human JC virus is ubiquitous in human populations and is reactivated frequently in immunosuppressed patients. Fifty-one patients with autoimmune disease receiving immunomodulating therapy were evaluated to study the possible relationship between immunosuppression and JCV viruria. Patients were divided into cytotoxic a...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/1096-9071(200012)62:4<505::aid-jmv16>3.0.c
更新日期:2000-12-01 00:00:00
abstract::Coronavirus disease 2019 (COVID-19) has become a serious public health problem worldwide. Here, we stratified COVID-19 patients based on their comorbidities to assess their risk of serious adverse outcomes. We collected 856 hospitalized cases diagnosed with COVID-19 from 17 January to 7 February 2020, in Zhejiang Prov...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26183
更新日期:2020-11-01 00:00:00
abstract::Seasonal fluctuations in hepatitis A have been observed in the Netherlands related to Turkish and Moroccan children after visiting their home countries. This study determined the prevalence and associated factors of hepatitis A virus (HAV) antibodies in Turkish and Moroccan children in Rotterdam. A random sample was t...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10576
更新日期:2004-02-01 00:00:00
abstract::The effect on cellular (c) oncogene RNA levels was investigated after infection of permissive cells with cell culture adapted strains (AD-169, C-87, Davis) and unadapted clinical isolates (82-1, 84-2, 85-1) of human cytomegalovirus (HCMV). The results indicate that both adapted and unadapted strains of HCMV induce sub...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890340409
更新日期:1991-08-01 00:00:00
abstract::Nucleotide sequences of the hypervariable region of hepatitis C virus genomes obtained from plasma change rapidly during the course of infection and are believed to play a part in immunological escape and consequently in the development of persistent infection. It is not known, however, whether these changes also occu...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890460103
更新日期:1995-05-01 00:00:00
abstract::GB virus C (GBV-C) is related to hepatitis C virus (HCV) and has a similar genomic structure. Some predictors for the efficacy of interferon (IFN) therapy on HCV have been reported: genotype, viral load, IFN dose, and the amino acid substitutions in the NS5A region, designated as the interferon sensitivity determining...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(199904)57:4<376::aid-jmv9>
更新日期:1999-04-01 00:00:00