Abstract:
:Five temperature-sensitive mutants (tsm9, tsm13, tsm20, tsm22, tsm30) of murine cytomegalovirus have been shown previously not to produce infectious virus in mice. In the present study, the stage at which these mutants are blocked in their replication in vitro was examined by transcriptional analysis of 4 temporally regulated marker genes (IE-1, E-1, gB and gH) using a semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) coupled with an electron microscopic analysis of infected cells incubated at permissive (33 degrees C) and non-permissive (39 and/or 40 degrees C) temperatures. Replication of tsm13 appeared to be blocked at a late phase of replication after capsid formation while the block appeared to be as early as the immediate-early phase in tsm22- infected cells. In contrast, mutants tsm9, tsm20 and tsm30 were blocked at a maturation step, probably of capsid formation, as gene transcription of all 4 marker genes occurred, albeit at reduced level, at 39 and 40 degrees C but no capsids or virions were produced at 40 degrees C. Replication and transcription of mutants tsm13, tsm20 and tsm30 were also examined in infected mice. Mutant tsm13 showed no gene expression or infectious virus while mutants tsm20 and tsm30 produced no infectious virus from days 3-60 post infection, except unusually for a low titre of tsm30 (2.3 x 10(3) pfu/ml) in salivary glands 21 days post infection. Gene transcription of all 4 marker genes was observed in one or more tissues (salivary glands, spleen, kidneys, liver, thymus, heart, lungs) at one or more time points (3, 7, 10, 14, 21 days post-infection) with both mutants. Mice became infected latently with tsm20 but not tsm30, and mice previously infected with tsm20 or tsm30 were protected against a sub-lethal challenge with virulent parental virus; tsm30 also protected against a lethal challenge. This suggests that these two mutants may be good model vaccines for further studies on the mechanism of protection induced and for identification of the ts genes.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Gill TA,Morley PJ,Sweet Cdoi
10.1002/1096-9071(200010)62:2<127::aid-jmv2>3.0.cokeywords:
subject
Has Abstractpub_date
2000-10-01 00:00:00pages
127-39issue
2eissn
0146-6615issn
1096-9071pii
10.1002/1096-9071(200010)62:2<127::AID-JMV2>3.0.COjournal_volume
62pub_type
杂志文章abstract::Although risk factors for cirrhosis in chronic hepatitis C virus (HCV) infection have been identified, the role of HCV-genotype 3 remains controversial, and limited data are available in drug users. The aim of the study was to assess risk factors for severe liver disease (cirrhosis/hepatocellular carcinoma) in HCV-inf...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21850
更新日期:2010-10-01 00:00:00
abstract::The prevalence of delta agent among 120 Chinese patients with HBsAg-positive chronic active and hepatocellular carcinoma from Taiwan and Los Angeles were studied by serological assay for antibody to delta antigen and immunoperoxidase staining for delta antigen in the liver biopsies. Of 120 patients, only four with chr...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890140106
更新日期:1984-01-01 00:00:00
abstract::Nineteen Chinese patients with chronic hepatitis B virus (HBV) infection, seropositive for HBV e antigen (HBeAg) and HBV DNA on at least three occasions in 6 months, were randomised to receive either recombinant human interferon-gamma (rIFN gamma) 0.1 mg/m2 intramuscularly thrice weekly for 16 weeks (n = 11) or no ant...
journal_title:Journal of medical virology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1002/jmv.1890340310
更新日期:1991-07-01 00:00:00
abstract::Ultra violet (UV) light-inactivated measles virus was used for the lymphocyte proliferation assay, where it caused consistently adult human peripheral blood monolymphocytes (PBML) proliferation which was not dependent on the measles virus complement fixation titre in the donor's sera. When the cord blood lymphocytes f...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890460209
更新日期:1995-06-01 00:00:00
abstract::Herpes simplex virus (HSV)-specific IgG and IgA antibody response in patients with HSV infection and in healthy adults was studied by the immunoperoxidase antibody-membrane antigen (IPAMA) technique. In all HSV infections in which specific IgM antibodies were detected by enzyme-linked immunosorbent assay (ELISA), a si...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890140303
更新日期:1984-01-01 00:00:00
abstract::Epstein-Barr virus is present in the saliva of most persistently infected individuals and is generally thought to be spread by close oral contact. However, there are now several reports of EBV in genital secretions, suggesting the possibility of sexual transmission between adults. The present study was undertaken to i...
journal_title:Journal of medical virology
pub_type: 杂志文章,多中心研究
doi:10.1002/jmv.20682
更新日期:2006-09-01 00:00:00
abstract::An enzyme-linked immunosorbent assay (ELISA) test using polyvalent antigens and antisera was used to detect Coxsackie B virus-specific IgM responses in 329 patients admitted to the Coronary Care Unit, Wellington Hospital, New Zealand over a 12-month period. The sera of 30 of 153 (19.6%) patients with acute myocardial ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890180211
更新日期:1986-02-01 00:00:00
abstract::Monoclonal antibodies were used for detecting varicella-zoster virus (VZV) by immunofluorescence (IF) in clinical specimens inoculated on shell vial cultures. Vesicles of 74 patients with varicella or herpes zoster-like eruptions were tested by this method and by conventional cell culture. Further diagnostic tests wer...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890280102
更新日期:1989-05-01 00:00:00
abstract::The detection of hepatitis B virus (HBV) DNA plays a critical role in determining the level of viral replication in HBV-infected patients. However, how to select appropriate HBV DNA detection method, low-sensitivity (ls) and hypersensitivity (hs) remains unclear. In this study, hepatitis B surface antigen (HBsAg), hep...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25271
更新日期:2019-02-01 00:00:00
abstract::Enterovirus infections are usually mild but can also cause severe illnesses and play a role in chronic diseases, such as cardiomyopathies and type 1 diabetes. Host response to the invading virus can markedly modulate the course of the infection, and this response varies between individuals due to the polymorphism of i...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23903
更新日期:2014-08-01 00:00:00
abstract::We investigated the molecular epidemiology and biological characteristics of wild measles viruses isolated since 1984 in Japan. The circulating measles virus was of genotype C1 before 1985, D3 from 1987 to 1990, and D5 from 1990 to 1997. It was replaced by the same cluster of Chicago-type D3 strain from 1997 to 1999. ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10443
更新日期:2003-08-01 00:00:00
abstract::Human cytomegalovirus (HCMV) is a major cause of morbidity and mortality in immunocompromised patients, such as renal transplant recipients. Analysis of the gene encoding the envelope glycoprotein B (gB) showed that clinical isolates adopted one of the sequence configurations, permitting the isolates to be assigned a ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10383
更新日期:2003-06-01 00:00:00
abstract::Human bocavirus (HBOV) has been reported as a worldwide distributed respiratory pathogen. It has also been associated with encephalitis recently by detection of the virus in cerebrospinal fluid (CSF) of patients presented with encephalitis. This retrospective study aimed to present clinical features of HBOV infections...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24263
更新日期:2015-11-01 00:00:00
abstract::Chronic papillary conjunctivitis has been described following adenoviral conjunctivitis. It is unknown however, how long adenovirus is able to persist in the tear film and conjunctiva. To determine if adenovirus persists in the ocular surface following adenoviral conjunctivitis, 304 patients with a history of adenovir...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20440
更新日期:2005-10-01 00:00:00
abstract::A diabetogenic strain of coxsackievirus B4 of human origin has been purified to study its biochemistry and diabetogenicity. Tissue culture cells infected with the virus contain two distinct types of particles--virions and membrane-bound virions (MBV). MBVs are lighter (p = 1.29) than virions (p = 1.34), and they conta...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890260109
更新日期:1988-09-01 00:00:00
abstract::Although hepatitis E virus (HEV) infection is a zoonosis, there is, currently, no standardized assay for quantitatively measuring anti-HEV antibody levels in HEV animal reservoirs. In this study, anti-HEV antibody positive serum from a rabbit (RS26) was calibrated by dose-response parallel line assay using the World H...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24651
更新日期:2017-03-01 00:00:00
abstract::Group A rotaviruses are the main cause of acute gastroenteritis in children worldwide. The intermediate capsid protein VP6 encoded by segment 6 of the dsRNA genome is the major structural component of the virus and it is highly antigenic and immunogenic. VP6 is responsible for group and subgroup (SG) specificities, al...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21306
更新日期:2008-11-01 00:00:00
abstract::A recent cohort study1 at Suizhou in China identified that the proportion of subjects who wore eyeglasses was lower in hospitalized patients with coronavirus disease 2019 (COVID-19) than in the general population. This article is protected by copyright. All rights reserved. ...
journal_title:Journal of medical virology
pub_type: 信件
doi:10.1002/jmv.26845
更新日期:2021-02-02 00:00:00
abstract::Coronavirus-like particle(s) (CVLP) are faecal-derived pleomorphic membrane bound virus-like particles characterised by a fringe of club-shaped spikes that measure about 27 nm in length. The association of CVLP with a variety of social, clinical, and epidemiological factors was examined after a 69 month survey of faec...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890290404
更新日期:1989-12-01 00:00:00
abstract::To elucidate the mechanism of heterologous antibody responses to herpes simplex virus (HSV) and varicella-zoster virus (VZV) which occur in some patients with HSV or VZV infections, stronger evidence was sought for the existence of cross-reacting antibodies to these viruses, using antibody absorption procedures. Absor...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890090105
更新日期:1982-01-01 00:00:00
abstract::Human papilloma virus (HPV) infection is a precursor of cervical cancer. This study aimed to introduce a method to quantify the risk of cervical cancer resulting from infection by different HPV subtypes, to help guide patient treatment. Nucleic acid molecule flow-through hybridization and gene chip technology were use...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24280
更新日期:2015-12-01 00:00:00
abstract::Influenza is a public health burden, responsible for more than half a million deaths worldwide each year and explosive outbreaks in-hospital care units. At present, little is known about clinical characteristics and outcomes with nosocomial influenza infection. To assess clinical characteristics and outcome between no...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25652
更新日期:2020-08-01 00:00:00
abstract::As a consequence of the high prevalence of TorqueTeno virus (TTV) in blood donors, thalassemia patients frequently acquire various genotypes of this virus through therapeutic blood transfusions. At present, the clinical consequences of TTV infection remain indeterminate for these patients. Here, several hundred thalas...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21070
更新日期:2008-02-01 00:00:00
abstract::An epidemiological and molecular study of hepatitis C virus (HCV) infection was carried out in Brazilian hemodialysis centers. A total of 1,095 patients in all 15 hemodialysis centers in the State of Goiás, Brazil, were studied. All patients were interviewed for possible risk factors to HCV infection and serum samples...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20932
更新日期:2007-09-01 00:00:00
abstract::The performance of French virology laboratories belonging to the ANRS network has been assessed annually for 3 years. The performance of these laboratories was compared between the years 2002 and 2003. Ten and 7 coded samples were sent to 38 virology laboratories in 2002 and 45 virology laboratories in 2003, respectiv...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20522
更新日期:2006-02-01 00:00:00
abstract::Enteric adenoviruses are important etiological agents associated with sporadic infections and outbreaks of acute gastroenteritis in infants and children. Fecal samples were collected from 439 hospitalized patients in the years 2005-2007 from Pune, Aurangabad, and Nagpur cities of western India to identify the most pre...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21331
更新日期:2009-01-01 00:00:00
abstract::Respiratory tract infections are a principal cause of illness and mortality in children worldwide and mostly caused by viruses. In this study, the epidemiology of 11 respiratory RNA viruses was investigated in a cohort of hospitalized children at a tertiary referral center in Riyadh from February 2008 to March 2009 us...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24435
更新日期:2016-06-01 00:00:00
abstract::Human immunodeficiency virus (HIV) is the primary etiological agent of acquired immunodeficiency syndrome (AIDS). This virus can be transmitted by blood or blood products. Homosexual males (HM) are regarded as one of the high-risk groups for AIDS. Lymphocytes obtained from HM were used for cocultivation with fresh HIV...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890230404
更新日期:1987-12-01 00:00:00
abstract::In mid-December 2019, a disease caused by infection with severe acute respiratory syndrome coronavirus-2, which began in Wuhan, China, has spread throughout the country and many countries around the world. The number of children with coronavirus disease-2019 (COVID-19) has also increased significantly. Although inform...
journal_title:Journal of medical virology
pub_type: 杂志文章,评审
doi:10.1002/jmv.25807
更新日期:2020-07-01 00:00:00
abstract::Nasopharyngeal carcinoma is closely associated with Epstein-Barr virus (EBV) and the EBV encoded latent membrane protein-1 expression (LMP1) is commonly found in the tumour cells. LMP1 has been shown to be involved in modulation of cell growth in B cells but the biological properties of LMP1 expression in nasopharynge...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.2112
更新日期:2002-01-01 00:00:00