Surveillance of measles in the Sudan using filter paper blood samples.

Abstract:

:Dried blood spots collected on filter paper are considered potential clinical specimens for measles surveillance because of their ease of collection, storage, and transport. The usefulness of these samples for surveillance of measles was evaluated in a field setting. Blood spots were collected by finger-prick from 316 clinically diagnosed measles patients in suburban Khartoum, mostly within a week after onset of the rash. Samples were collected between October, 2000 and April, 2003, and stored at 4 degrees C. Measles virus-specific IgM antibodies were detected in 200 (63%) of the samples using an "in-house" IgM capture ELISA. For 201 samples reconstitution and IgM measurement was repeated 1 year after initial testing with essentially the same results, showing the stability of IgM in the filter paper under these conditions. In a limited number of samples (n = 38) measles virus-specific IgM was also tested with a commercial indirect IgM ELISA. Although the results of the two assays correlated well, the "in-house" IgM capture ELISA proved slightly more sensitive. Measles virus-specific reverse transcriptase polymerase chain reaction (RT-PCR) amplicons were obtained from 16 of 57 (28%) samples tested. Sequencing of the 3' 456 nucleotides of the nucleoprotein gene showed the continued endemic circulation of genotype B3 viruses identified previously in this region. Although problems related to limited sample quantities were encountered, the present study confirms the usefulness of dried blood spots for measles surveillance. The results also demonstrate that measles continues to be endemic in the Sudan.

journal_name

J Med Virol

authors

El Mubarak HS,Yüksel S,Mustafa OM,Ibrahim SA,Osterhaus AD,de Swart RL

doi

10.1002/jmv.20136

keywords:

subject

Has Abstract

pub_date

2004-08-01 00:00:00

pages

624-30

issue

4

eissn

0146-6615

issn

1096-9071

journal_volume

73

pub_type

杂志文章
  • Low prevalence of IgG antibodies against antigens of HEV genotypes 1 and 3 in women with a high-risk pregnancy.

    abstract::The aim of the study was to assess whether high-risk pregnant women have a higher prevalence of HEV during the perinatal period. This was a cross-sectional study of 428 patients: Group 1, 127 women with a high-risk pregnancy; Group 2, 97 asymptomatic people with reactivity to HCV or HBV; Group 3, 94 patients with clin...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.24878

    authors: Baptista-González H,Trueba-Gómez R,Rosenfeld-Mann F,Roque-Álvarez E,Méndez-Sánchez N

    更新日期:2017-11-01 00:00:00

  • TTV infection in children born to mothers infected with TTV but not with HBV, HCV, or HIV.

    abstract::The TT virus (TTV) was isolated recently from the serum of a patient with post-transfusion hepatitis. TTV infection is widespread in the general population, and its prevalence increases continuously with age. The pathogenic role of TTV in liver disease remains controversial, and the source of transmission is still unc...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20204

    authors: Komatsu H,Inui A,Sogo T,Kuroda K,Tanaka T,Fujisawa T

    更新日期:2004-11-01 00:00:00

  • Herpes viruses and duodenal ulcer disease.

    abstract::Antibodies to herpes simplex virus and Epstein-Barr virus were sought in the sera of 33 patients with endoscopically proved duodenal ulcer and 33 healthy controls. Neither the prevalence nor the antibody levels to both viruses were significantly different between the two groups. These results suggest that herpes virus...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890290314

    authors: Kottaridis SD,Mihas TA,Goula I,Mihas AA

    更新日期:1989-11-01 00:00:00

  • Genotype I hepatitis A virus introduction in Italy: Bayesian phylogenetic analysis to date different epidemics.

    abstract::Despite a significant decrease in acute hepatitis A in the last 2 decades in Italy, outbreaks were observed occurring mostly in southern Italy. In this study, Bayesian phylogenetic analysis was used to analyze the origin of these epidemics. With this aim, 5 different data sets of hepatitis A virus sequences were built...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.25220

    authors: Cella E,Riva E,Angeletti S,Fogolari M,Blasi A,Scolamacchia V,Spoto S,Bazzardi R,Lai A,Sagnelli C,Sagnelli E,Ciccozzi M

    更新日期:2018-09-01 00:00:00

  • Neutralization epitope diversity of coxsackievirus B4 isolates detected by monoclonal antibodies.

    abstract::Only recently have we begun to fully realize the diversity of virions within a single human isolate of the group B Coxsackieviruses. These intratypic (strain) differences may be one of the important factors influencing pathogenesis, e.g., myocarditis vs. pancreatitis. Yet, until the virion strains within a type can be...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890200103

    authors: Webb SR,Kearse KP,Foulke CL,Hartig PC,Prabhakar BS

    更新日期:1986-09-01 00:00:00

  • A simple method for detection of a novel coronavirus (SARS-CoV-2) using one-step RT-PCR followed by restriction fragment length polymorphism.

    abstract::A novel coronavirus associated with acute respiratory disease (named SARS-CoV-2) is recently identified in Wuhan city, China, spread rapidly worldwide. Early identification of this novel coronavirus by molecular tools is critical for surveillance and control of the epidemic outbreak. We aimed to establish a simple met...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.26171

    authors: Son HA,Hang DTT,Thuan ND,Quyen LTB,Thuong LTH,Nga VT,Quang LB,Hung TT,Son NT,Linh NT,Nam LV,Van Ba N,Tien TV,Quyet D,Van Luong H,Su HX

    更新日期:2020-11-01 00:00:00

  • Identification of epitopes in the nucleocapsid protein of Nipah virus using a linear phage-displayed random peptide library.

    abstract::A random peptide library of heptamers displayed on the surface of M13 bacteriophage was used to identify specific epitopes of antibodies in pooled sera of swine naturally infected by Nipah virus. The selected heptapeptides were aligned with protein sequences of Nipah virus and several putative epitopes were identified...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20249

    authors: Eshaghi M,Tan WS,Yusoff K

    更新日期:2005-01-01 00:00:00

  • The role of DCs in the immunopathogenesis of chronic HBV infection and the methods of inducing DCs maturation.

    abstract::Chronic hepatitis B virus (HBV) infection is the result of an inadequate immune response towards the virus. Dendritic cells (DCs), as the most efficient professional antigen-presenting cells (APCs), possess the strongest antigen presenting the effect in the body and can stimulate the initial T cell activation and prol...

    journal_title:Journal of medical virology

    pub_type: 杂志文章,评审

    doi:10.1002/jmv.24306

    authors: Sun HH,Zhou DF,Zhou JY

    更新日期:2016-01-01 00:00:00

  • Identification of a novel human papillomavirus type 16 E1 gene variant with potentially reduced oncogenicity.

    abstract::The human papillomavirus (HPV) 16 genome has been studied extensively, although no study has focused on the E1 gene that is implicated in viral DNA replication. After analyzing the E1 region of HPV 16 genomes in 429 cervical samples, 11.2% were found to contain a 63 nucleotides duplication in this region. Sequence ana...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21304

    authors: Sabol I,Matovina M,Gasperov NM,Grce M

    更新日期:2008-12-01 00:00:00

  • Detection of HPV types and neutralizing antibodies in Gansu province, China.

    abstract::A total of 82 samples from patients with cervical cancer (Group 1) and 50 samples from patients with other genital diseases (Group 2) were collected in Gansu, China. All 132 samples were tested for HPV DNA with a typing kit that can detect 21 types of HPV, and also tested for neutralizing antibodies against HPV-16, -1...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21435

    authors: Wu X,Zhang C,Feng S,Liu C,Li Y,Yang Y,Gao J,Li H,Meng S,Li L,Zhang Y,Hu X,Wu X,Lin L,Li X,Wang Y

    更新日期:2009-04-01 00:00:00

  • Spectrum of hepatitis E virus infection in India.

    abstract::A solid phase enzyme linked immunosorbent assay (ELISA) that detects IgM and IgG to hepatitis E virus (HEV) was used to study seroepidemiology in 40 healthy subjects and 227 consecutive patients with liver diseases in an endemic area. Fifty-two of the liver diseases patients (22.9 percent) had acute hepatitis E. In co...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890430316

    authors: Khuroo MS,Rustgi VK,Dawson GJ,Mushahwar IK,Yattoo GN,Kamili S,Khan BA

    更新日期:1994-07-01 00:00:00

  • Fulminant human herpesvirus six encephalitis in a human immunodeficiency virus-infected infant.

    abstract::Self-limited involvement of the central nervous system (CNS) is a relatively common complication of primary infection with human herpesvirus six (HHV-6) in normal children. We describe an HIV-infected infant who developed fulminant encephalitis as a complication of HHV-6 infection. Immunohistochemical staining of CNS ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890450309

    authors: Knox KK,Harrington DP,Carrigan DR

    更新日期:1995-03-01 00:00:00

  • A chemiluminescent, magnetic particle-based immunoassay for the detection of hepatitis C virus core antigen in human serum or plasma.

    abstract::Hepatitis C virus (HCV) exposure in blood donors is determined serologically by the detection of anti-HCV antibodies in serum or plasma. However, a "window" period of 30-70 days after exposure exists where specific antibodies to HCV antigens are not detected. The use of nucleic acid testing for the detection of HCV RN...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20716

    authors: Leary TP,Gutierrez RA,Muerhoff AS,Birkenmeyer LG,Desai SM,Dawson GJ

    更新日期:2006-11-01 00:00:00

  • Longitudinal use of phenotypic resistance testing to HIV-1 protease inhibitors in patients developing HAART failure.

    abstract::An "in-house" recombinant virus protease inhibitor susceptibility assay was carried out (median of 3 per patient) retrospectively in 26 patients failing HIV protease inhibitor based therapy at regular intervals to the initiation of the first protease inhibitor. Patients were treated with either indinavir (N = 6), rito...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.10076

    authors: Servais J,Plesséria JM,Lambert C,Fontaine E,Robert I,Arendt V,Staub T,Schneider F,Hemmer R,Schmit JC

    更新日期:2002-07-01 00:00:00

  • Hepatitis C viraemia rebound after "successful" interferon therapy in patients with chronic non-A, non-B hepatitis.

    abstract::A quantitative polymerase chain reaction (PCR) assay for hepatitis C viral RNA (HCV-RNA) was used to monitor viraemia levels in six patients at multiple time points before, during, and after interferon therapy for chronic non-A, non-B hepatitis (NANBH). Prior to therapy, serum HCV-RNA was detected in all patients at a...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890370311

    authors: Garson JA,Brillanti S,Ring C,Perini P,Miglioli M,Barbara L

    更新日期:1992-07-01 00:00:00

  • Seroepidemiological survey of hepatitis B and C virus infections in Ghanaian children.

    abstract::The seroprevalences of hepatitis B virus (HBV) and hepatitis C virus (HCV) markers were evaluated in a random sample of 803 children attending school in Ashanti-Akim North district in Ghana in order to gain a better understanding of transmission patterns of these viruses, particularly horizontal transmission of HBV. T...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1096-9071(199603)48:3<278::AID-JMV11

    authors: Martinson FE,Weigle KA,Mushahwar IK,Weber DJ,Royce R,Lemon SM

    更新日期:1996-03-01 00:00:00

  • Nitric oxide and Coxsackievirus B3 myocarditis: differential expression of inducible nitric oxide synthase in mouse heart after infection with virulent or attenuated virus.

    abstract::Increased expression of inducible nitric oxide synthase (iNOS) has been found in inflammatory myocardial disease and increased production of nitric oxide (NO) has both an inhibitory effect on virus replication and a cytotoxic effect on host cells. To investigate the relationship between severity of enteroviral myocard...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1033

    authors: Bevan AL,Zhang H,Li Y,Archard LC

    更新日期:2001-06-01 00:00:00

  • Combinations of 1,8-cineol and oseltamivir for the treatment of influenza virus A (H3N2) infection in mice.

    abstract::It is need for development of new means against influenza virus due to the lack of efficacy of available therapeutic strategies. In previous research, 1,8-cineol exert its inhibition of nuclear factor (NF)-κB, the main regulator of cytokine and chemokine production in influenza, and anti-inflammatory activity. These f...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.24755

    authors: Lai YN,Li Y,Fu LC,Zhao F,Liu N,Zhang FX,Xu PP

    更新日期:2017-07-01 00:00:00

  • Detection of hepatitis B virus in serum using amplification of viral DNA by means of the polymerase chain reaction.

    abstract::A new assay was developed for the detection of hepatitis B virus (HBV) in human serum using amplification of a short viral DNA sequence by means of the polymerase chain reaction. As little as 0.4 fg viral DNA, corresponding to about 130 genome equivalents, per ml serum could be detected after the amplification procedu...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890270409

    authors: Sumazaki R,Motz M,Wolf H,Heinig J,Jilg W,Deinhardt F

    更新日期:1989-04-01 00:00:00

  • Infection of simian B lymphoblastoid cells with simian immunodeficiency virus is associated with upregulation of CD23 and CD40 cell surface markers.

    abstract::Simian immunodeficiency virus (SIV) as well as human immunodeficiency virus (HIV) induce polyclonal B-cell activation and are associated with the appearance of lymphomas in their respective hosts in either the presence or the absence of other co-infecting viruses such as Epstein-Barr virus (EBV). However, the pathogen...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.10179

    authors: Titti F,Zamarchi R,Maggiorella MT,Sernicola L,Geraci A,Negri DR,Borsetti A,Menin C,D'Andrea E,Modesti A,Masuelli L,Verani P,Chieco-Bianchi L,Amadori A

    更新日期:2002-09-01 00:00:00

  • Frequent detection of cell-associated HIV-1 RNA in patients with plasma viral load

    abstract::Despite prolonged undetectable plasma viral load some HIV-1 infected patients have been reported to develop resistance-associated mutations leading to treatment failure. The mechanisms for this phenomenon and the point of origin for residual viral evolution are still not elucidated. In order to quantify cell-associate...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20993

    authors: Kupfer B,Matz B,Däumer MP,Roden F,Rockstroh JK,Qurishi N,Spengler U,Kaiser R

    更新日期:2007-10-01 00:00:00

  • Profile of pre-S deletions in the natural history of chronic hepatitis B infection.

    abstract::It have been suggested that hepatitis B virus (HBV) pre-S deletions may play a role in hepatocarcinogenesis. The aim of the study was to determine the prevalence of pre-S deletions in chronic hepatitis B patients in Hong Kong, the factors associated with the deletions and its relationship with hepatitis B e antigen (H...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21901

    authors: Yeung P,Wong DK,Lai CL,Fung J,Seto WK,Yuen MF

    更新日期:2010-11-01 00:00:00

  • Comparison of the hypervariable region of hepatitis C virus genomes in plasma and liver.

    abstract::Nucleotide sequences of the hypervariable region of hepatitis C virus genomes obtained from plasma change rapidly during the course of infection and are believed to play a part in immunological escape and consequently in the development of persistent infection. It is not known, however, whether these changes also occu...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890460103

    authors: Sakamoto N,Enomoto N,Kurosaki M,Asahina Y,Maekawa S,Koizumi K,Sakuma I,Murakami T,Marumo F,Sato C

    更新日期:1995-05-01 00:00:00

  • Epidemiology of Varicella-Zoster Virus in England and Wales.

    abstract::Many countries are studying currently the possibility of mass vaccination against varicella. The objective of this study was to provide a complete picture of the pre-vaccine epidemiology of the Varicella-Zoster Virus in England and Wales to aid in the design of immunisation programs. Population-based data including ge...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.10313

    authors: Brisson M,Edmunds WJ

    更新日期:2003-01-01 00:00:00

  • Impact of human metapneumovirus and human cytomegalovirus versus other respiratory viruses on the lower respiratory tract infections of lung transplant recipients.

    abstract:UNLABELLED:Viral respiratory tract infections in lung transplant recipients may be severe. During three consecutive winter-spring seasons, 49 symptomatic lung transplant recipients with suspected respiratory viral infection, and 26 asymptomatic patients were investigated for presence of respiratory viruses either in 56...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20555

    authors: Gerna G,Vitulo P,Rovida F,Lilleri D,Pellegrini C,Oggionni T,Campanini G,Baldanti F,Revello MG

    更新日期:2006-03-01 00:00:00

  • Evidence for persistence of parvovirus B19 DNA in livers of adults.

    abstract::Recent studies have suggested a pathogenic role of human parvovirus B19 (B19) in the development of acute fulminant liver failure in children. The hypothesis was based on the detection of B19 DNA in 8 of 10 explanted livers of children requiring liver transplantation. In the present study, explanted livers from 43 adu...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.2047

    authors: Eis-Hübinger AM,Reber U,Abdul-Nour T,Glatzel U,Lauschke H,Pütz U

    更新日期:2001-10-01 00:00:00

  • Evaluation of LIAISON® XL system for HBsAg, and anti-HCV and anti-HIV/Ag p24.

    abstract::The aim of this study was to compare the data obtained using the new LIAISON® XL chemiluminescence system to search for HBsAg, anti-HCV, and anti-HIV1-2/p24 Ag with those obtained using the VITROS system currently adopted by the Microbiology Unit of the Hospital of Legnano. Routine samples of patients who were referre...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.24648

    authors: De Paschale M,Manco MT,Belvisi L,Cagnin D,Cerulli T,Paganini A,Arpino O,Cianflone A,Agrappi C,Mirri P,Clerici P

    更新日期:2017-03-01 00:00:00

  • Human hepatitis B virus X protein augments the DNA binding of nuclear factor for IL-6 through its basic-leucine zipper domain.

    abstract::The X gene product of human hepatitis B virus, HBx, transactivates the expression of viral and cellular genes through a wide variety of cis elements, including the nuclear factor for IL-6 (NF-IL6) binding sites, although HBx does not appear to bind DNA directly. We previously reported that HBx transactivated the inter...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/(sici)1096-9071(199905)58:1<11::aid-jmv2>3

    authors: Ohno H,Kaneko S,Lin Y,Kobayashi K,Murakami S

    更新日期:1999-05-01 00:00:00

  • Physicochemical heterogeneity of hepatitis B e antigen detected in asymptomatic carriers and carriers in a hemodialysis unit.

    abstract::Physicochemical studies of hepatitis B e antigen (HBeAg) revealed a clear cut difference between e1 and e2 antigen. The e1 antigen was found to have a MW of Ca 150,000 and a pI of 6.4-7.2, whereas both the MW and pI of the e2 antigen were heterogeneous depending upon the source of serum. Sera obtained from asymptomati...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890040105

    authors: Yamada E,Ohori H,Ishida N

    更新日期:1979-01-01 00:00:00

  • Human immune response to Puumala virus glycoproteins and nucleocapsid protein expressed in mammalian cells.

    abstract::Puumala hantavirus (PUUV) glycoproteins G1 and G2 and nucleocapsid protein (N) were expressed in BHK-21 cells by transfection of a plasmid producing a recombinant alphavirus replicon. Coexpression of G1 and G2 from separate constructs seemed to be important for the optimal folding of the glycoproteins, as evaluated by...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:

    authors: Kallio-Kokko H,Leveelahti R,Brummer-Korvenkontio M,Lundkvist A,Vaheri A,Vapalahti O

    更新日期:2001-11-01 00:00:00