Abstract:
:A new assay was developed for the detection of hepatitis B virus (HBV) in human serum using amplification of a short viral DNA sequence by means of the polymerase chain reaction. As little as 0.4 fg viral DNA, corresponding to about 130 genome equivalents, per ml serum could be detected after the amplification procedure. This assay detected viral DNA in a number of patients with proven or suspected chronic HBV infection who were all negative for HBV DNA in the conventional hybridisation assay. We found HBV DNA in all of six HBeAg-positive and in three of eight HBeAg-negative HBsAg carriers, as well as in all of 11 patients with chronic liver disease with antibodies against the HBV core antigen (anti-HBc) as the sole marker for HBV infection, and in three of five apparently healthy individuals showing only anti HBc. Thus, this method is an important improvement for the diagnosis of persistent HBV infections, especially in patients where a definitive serological diagnosis is not possible.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Sumazaki R,Motz M,Wolf H,Heinig J,Jilg W,Deinhardt Fdoi
10.1002/jmv.1890270409subject
Has Abstractpub_date
1989-04-01 00:00:00pages
304-8issue
4eissn
0146-6615issn
1096-9071journal_volume
27pub_type
杂志文章abstract::Simian immunodeficiency virus (SIV) as well as human immunodeficiency virus (HIV) induce polyclonal B-cell activation and are associated with the appearance of lymphomas in their respective hosts in either the presence or the absence of other co-infecting viruses such as Epstein-Barr virus (EBV). However, the pathogen...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10179
更新日期:2002-09-01 00:00:00
abstract::In order to assess the efficacy of interferon-alpha on hepatitis C viral RNA in patients with chronic hepatitis C, we analyzed the levels of HCV RNA in sera and liver tissues of 16 patients pre- and posttreatment using a semiquantitative polymerase chain reaction method. Fifteen of 16 patients were positive for anti-H...
journal_title:Journal of medical virology
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1002/jmv.1890400114
更新日期:1993-05-01 00:00:00
abstract::The purpose of this study was to investigate determinants of endemic hepatitis C virus (HCV) infection within communities in Taiwan. A two-phase study, including a seroprevalence survey and a prevalent case-control study at the first phase, which has been published previously, and a follow-up seroconversion determinat...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:2001-09-01 00:00:00
abstract::Interaction between herpesviruses and human immunodeficiency virus (HIV)1 is postulated in the progression of HIV disease. In order to evaluate the specific antibody responses directed to Epstein-Barr virus (EBV) and cytomegalovirus (CMV) and to provide serological evidence suggesting reactivation of these viruses abl...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890360112
更新日期:1992-01-01 00:00:00
abstract::Hepatitis E virus (HEV) infection is a significant public health issue in many developing countries, causing waterborne outbreaks as well as sporadic hepatitis. We report here an outbreak of HEV genotype 1f infection during April-May 2018 among people living at Halisohor, a low land in the southern part of Chottogram ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26739
更新日期:2020-12-17 00:00:00
abstract::Varicella in the immunocompetent adult is an infrequent but potentially serious infection. Previous studies in immunocompetent hosts and normal adults have demonstrated the value of intravenous acyclovir in the treatment of varicella-zoster virus infections. Oral acyclovir has also shown efficacy in both normal adults...
journal_title:Journal of medical virology
pub_type: 杂志文章,评审
doi:10.1002/jmv.1890410517
更新日期:1993-01-01 00:00:00
abstract::The 2020 coronavirus pandemic is developing at different paces throughout the world. Some areas, like the Caribbean Basin, have yet to see the virus strike at full force. When it does, there is reasonable evidence to suggest the consequent COVID-19 outbreaks will overwhelm healthcare systems and economies. This is par...
journal_title:Journal of medical virology
pub_type: 杂志文章,评审
doi:10.1002/jmv.25864
更新日期:2020-09-01 00:00:00
abstract::Knowledge of the precise timing of SARS-CoV-2 infection may be of clinical and epidemiological relevance. The presence of low-avidity IgGs has conventionally been considered an indicator of recent infection. Here, we carried out qualitative assessment of SARS-CoV-2-specific antibody avidity using an urea (6M) dissocia...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26344
更新日期:2021-02-01 00:00:00
abstract::HIV-1 replicative activity and its relation to the clinical and immunological evolution of infection was studied in a group of 150 HIV-1 seropositive Italian i.v. drug abusers over a 1 year period. HIV-1 was isolated from 90 (60%) subjects; two groups of isolates were distinguished, according to replicative activity "...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890320411
更新日期:1990-12-01 00:00:00
abstract::Annual outbreaks of seasonal influenza cause a substantial health burden. The aim of this study was to compare patient demographic/clinical data in two influenza patient groups presenting to hospital; those requiring O2 or critical care admission and those requiring less intensive treatment. The study was conducted fr...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26376
更新日期:2020-07-31 00:00:00
abstract::A quantitative polymerase chain reaction (PCR) assay for hepatitis C viral RNA (HCV-RNA) was used to monitor viraemia levels in six patients at multiple time points before, during, and after interferon therapy for chronic non-A, non-B hepatitis (NANBH). Prior to therapy, serum HCV-RNA was detected in all patients at a...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890370311
更新日期:1992-07-01 00:00:00
abstract::A molecular epidemiological study on common diarrheal viruses was conducted in Ho Chi Minh City, Vietnam between October 2002 and September 2003. Fecal samples were collected from 1,010 hospitalized children with acute gastroenteritis. Those samples were screened for groups A, B, and C rotavirus, adenovirus, genogroup...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20857
更新日期:2007-05-01 00:00:00
abstract::Noroviruses (called formerly "Norwalk-like viruses") cause food-borne gastroenteritis outbreaks. These outbreaks were thought to be related to shellfish consumption, although non-shellfish related outbreaks also occurred frequently in Japan. To clarify the epidemiology of Norovirus outbreaks, 435 stool samples were co...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20455
更新日期:2005-10-01 00:00:00
abstract::Hepatitis C virus (HCV) exposure in blood donors is determined serologically by the detection of anti-HCV antibodies in serum or plasma. However, a "window" period of 30-70 days after exposure exists where specific antibodies to HCV antigens are not detected. The use of nucleic acid testing for the detection of HCV RN...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20716
更新日期:2006-11-01 00:00:00
abstract::Hepatitis E virus sequences were detected by RT-PCR in 14/15 (93%) of untreated sewage samples from Edinburgh, Scotland, UK. Phylogenetic analysis of amplicons at limiting dilution revealed the co-circulation of multiple variants of HEV-3, with a pattern of diversity matching that observed in a local cohort of HEV-inf...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24403
更新日期:2016-05-01 00:00:00
abstract::Hepatitis B virus (HBV) infection is a dynamic process during which molecular variants are selected continuously to adapt to changes. In addition to drug resistant mutations, sequential antiviral therapy may also lead to the selection of deleted mutants. To investigate this process, the following samples were collecte...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21595
更新日期:2009-09-01 00:00:00
abstract::Human cytomegalovirus (HCMV) is an important opportunistic pathogen in immunocompromised individuals and is recognized as a major viral cause of birth defects. HCMV has the ability to establish lifelong persistence and latent infection following primary exposure. Apoptosis is an innate cellular defense response to vir...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24259
更新日期:2015-11-01 00:00:00
abstract::To assess the prevalence of HPV infection in the genital tracts of women with normal PAP smears, a random series of 109 women was reexamined using colposcopy, a further PAP smear, and punch biopsies taken from the cervix (in 33 cases), vagina (212 cases), and anus (20 cases). The biopsy material was examined using rou...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890310404
更新日期:1990-08-01 00:00:00
abstract::Taiwan was a hyperendemic area for hepatitis A and B viruses (HAV and HBV) infection before late 1980s. To study the seroprevalence of hepatitis A, B, C, and E viruses (HCV and HEV) infection among preschool children in Taiwan, a community-based survey was carried out in 54 kindergartens in 10 urban areas, 10 rural ar...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20517
更新日期:2006-01-01 00:00:00
abstract::The prevalence rate of IgG antibodies to cytomegalovirus (CMV) was determined in a sample of 567 women of childbearing age in the southern part of Israel by the immunoperoxidase assay to membrane antigen (IPAMA) technique. Urban Jewish women of Afro-Asian origin showed significantly higher rates of seropositivity than...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890240104
更新日期:1988-01-01 00:00:00
abstract::Herpes simplex virus (HSV) establishes latency in neurons and recurrent infections in oral mucosa. This prospective study analyzes HSV prevalence in oral mucosal brush samples from men with known human papillomavirus (HPV) status. We hypothesized that HSV-1-infection could facilitate HPV persistence as a cofactor. Thi...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24965
更新日期:2018-03-01 00:00:00
abstract::Incarcerated persons have high rates of infectious diseases. Few data on the prevalence of sexually transmitted diseases in prisoners are available. This multi-center cross-sectional study enrolled 973 inmates from eight Italian prisons. Demographic and behavioral data were collected using an anonymous standardized qu...
journal_title:Journal of medical virology
pub_type: 杂志文章,多中心研究
doi:10.1002/jmv.20774
更新日期:2007-02-01 00:00:00
abstract::Analysis of the HIV-1 V3 quasispecies present in an individual at the time of seroconversion was carried out. The polymerase chain reaction (PCR) was used to amplify proviral HIV-1 DNA extracted from peripheral blood mononuclear cells from a patient who was viraemic (p24 = 15 pg/ml) and had an equivocal HIV-1 antibody...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890410403
更新日期:1993-12-01 00:00:00
abstract::A sensitive and specific nonradioactive in situ hybridization method capable of detecting single-copy integrated hepatitis B virus (HBV) DNA sequences in hepatocellular carcinoma (HCC) cell lines was developed. In situ hybridization of biotinylated HBV (adr, adw) DNA probes with nine different human HCC cell lines wer...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890300407
更新日期:1990-04-01 00:00:00
abstract::Nine ducks congenitally infected with the duck hepatitis B virus (DHBV) were treated either orally (four ducks for 10 weeks) or intraperitoneally (five ducks for 12 weeks) with the Indian traditional herbal remedy Phyllanthus amarus. Compared to placebo-treated control ducks, these treatments did not result in a reduc...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890320404
更新日期:1990-12-01 00:00:00
abstract::Nasal washings were collected from 27 normal adults during 38 naturally acquired colds. The washings were exhaustively tested using tissue cultures, organ cultures and electron microscopy. Washings yielding no identifiable agent were inoculated into human volunteers, and further specimens obtained from the latter were...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890050306
更新日期:1980-01-01 00:00:00
abstract::Identification of high-risk HPV genotypes in patients is essential for vaccination and prevention programs while the geographic distribution of cervical cancer varies widely. HPV 16 is the major cause of cervical cancer followed by HPV 18, HPV 31, HPV 52, or HPV 58 depending on geographic area. In this study, the dist...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23769
更新日期:2014-04-01 00:00:00
abstract::Currently, data examining nationally representative prevalence and trends of HBV or HCV among specific subgroups of pregnant women in the US are unavailable. We conducted a cross-sectional analysis of hospitalizations for liveborn singleton deliveries from 1998 to 2011 using data from the Nationwide Inpatient Sample. ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24725
更新日期:2017-06-01 00:00:00
abstract::Interferon alpha (IFN-alpha) therapy is currently the treatment of choice for chronic hepatitis C (HCV) infection, but it fails to achieve a sustained response in approximately 75% of those treated. The factors which determine whether or not an individual will respond to IFN-alpha are uncertain, although a number of p...
journal_title:Journal of medical virology
pub_type: 临床试验,杂志文章
doi:10.1002/jmv.1890450320
更新日期:1995-03-01 00:00:00
abstract::Hepatitis A virus infections have been reported recently among hemophilic patients in Italy and Germany, leading to speculation that infectious hepatitis A virus (HAV) might have been present in some factor VIII concentrates. In both cases, the implicated factor concentrates had been treated by a solvent/detergent met...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890410113
更新日期:1993-09-01 00:00:00