Abstract:
:A sensitive and specific nonradioactive in situ hybridization method capable of detecting single-copy integrated hepatitis B virus (HBV) DNA sequences in hepatocellular carcinoma (HCC) cell lines was developed. In situ hybridization of biotinylated HBV (adr, adw) DNA probes with nine different human HCC cell lines were carried out in 96-well microtiter plates. Integration was detected in HCC cell lines HCCM, Hep3B, huH-1, huH-4, and PLC/PRF/5. Detection of single-copy HBV DNA sequences was also achieved in Hep3B and huH-4. HCC cell lines HepG2, HUH-6, HUH-7, Mahlavu, and the non-HCC control MCF-7, gave clear negative results. These results show a 100% correlation with those obtained by Southern blot hybridization assay. The results demonstrate that nonradioactive detection of single-copy integrated HBV DNA sequences in HCC cell lines is possible by the method described, which has potential application for viral genome analysis requiring in situ hybridization.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Tay N,Chan SH,Ren ECdoi
10.1002/jmv.1890300407subject
Has Abstractpub_date
1990-04-01 00:00:00pages
266-71issue
4eissn
0146-6615issn
1096-9071journal_volume
30pub_type
杂志文章abstract::Alfa-fetoprotein (AFP) is used as a marker of early hepatocarcinogenesis. However, the impact of hepatitis B virus surface antigen (HBsAg) on this relationship in patients with HBV infection is not clear. The present study evaluated the relation between HBsAg and AFP levels at the initial visit in 1,610 untreated HBV ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23790
更新日期:2014-01-01 00:00:00
abstract::The plaque reduction neutralization test (PRNT) has been used routinely in serological studies with such arenaviruses as Junin, Machupo, and Parana. However, difficulties have been encountered in using the PRNT for LCM virus, while conflicting views have been expressed about the reliability and efficacy of the test wi...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890220110
更新日期:1987-05-01 00:00:00
abstract::Hepatitis D virus (HDV) infection can cause severe acute and chronic liver disease in patients infected with hepatitis B virus (HBV). Despite the significant decline in the global HDV infection, it remains a major health concern in some countries. This study aimed to investigate the prevalence and clinical features of...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.22095
更新日期:2011-07-01 00:00:00
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journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10239
更新日期:2002-12-01 00:00:00
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journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24118
更新日期:2015-05-01 00:00:00
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journal_title:Journal of medical virology
pub_type: 杂志文章
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更新日期:2001-07-01 00:00:00
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journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(200006)61:2<235::aid-jmv10
更新日期:2000-06-01 00:00:00
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journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890470304
更新日期:1995-11-01 00:00:00
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journal_title:Journal of medical virology
pub_type: 杂志文章
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更新日期:2010-05-01 00:00:00
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journal_title:Journal of medical virology
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更新日期:2013-09-01 00:00:00
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journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890370403
更新日期:1992-08-01 00:00:00
abstract::A recent cohort study1 at Suizhou in China identified that the proportion of subjects who wore eyeglasses was lower in hospitalized patients with coronavirus disease 2019 (COVID-19) than in the general population. This article is protected by copyright. All rights reserved. ...
journal_title:Journal of medical virology
pub_type: 信件
doi:10.1002/jmv.26845
更新日期:2021-02-02 00:00:00
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journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23867
更新日期:2014-04-01 00:00:00
abstract::The performance of French virology laboratories belonging to the ANRS network has been assessed annually for 3 years. The performance of these laboratories was compared between the years 2002 and 2003. Ten and 7 coded samples were sent to 38 virology laboratories in 2002 and 45 virology laboratories in 2003, respectiv...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20522
更新日期:2006-02-01 00:00:00
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journal_title:Journal of medical virology
pub_type: 杂志文章,评审
doi:10.1002/jmv.25864
更新日期:2020-09-01 00:00:00
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journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890210209
更新日期:1987-02-01 00:00:00
abstract::GB virus C/hepatitis G virus (GBV-C/HGV) is related distantly to hepatitis C virus (HCV). HCV has a hypervariable region (HVR), and exists as quasispecies in vivo. Although GBV-C/HGV also has replaceable amino acids in the presumed antigenic region, the existence and fluctuation of population of heterogeneous virus ha...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:1998-06-01 00:00:00
abstract::The presence of hepatitis GB virus C (GBV-C), also known as hepatitis G virus (HGV), and hepatitis C virus (HCV) were investigated in sera from 45 hemophiliacs from nine locations in Nicaragua using a nested polymerase chain reaction (PCR). Primers used to detect GBV-C and HCV derived from the helicase region and 5'UT...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:1997-06-01 00:00:00
abstract::Avidity maturation of IgG antibody responses directed against the structural proteins of rubella virus (E1, E2, and C) as well as whole rubella virus (RV) was assessed at sequential time intervals in 7 individuals following serologically confirmed wild rubella infection. Individual structural proteins were purified fr...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890360310
更新日期:1992-03-01 00:00:00
abstract::Human rhinovirus (HRV), a single-stranded, positive-sense RNA virus, is associated with mild upper respiratory tract infections in children. The aim of this study was to carry out a molecular characterization and phylogeny-based classification of the circulating genotypes of HRV in hospitalized children with clinical ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23638
更新日期:2013-09-01 00:00:00
abstract::As a consequence of the high prevalence of TorqueTeno virus (TTV) in blood donors, thalassemia patients frequently acquire various genotypes of this virus through therapeutic blood transfusions. At present, the clinical consequences of TTV infection remain indeterminate for these patients. Here, several hundred thalas...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21070
更新日期:2008-02-01 00:00:00
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journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25079
更新日期:2018-12-01 00:00:00
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journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23346
更新日期:2012-09-01 00:00:00
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journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(199706)52:2<206::aid-jmv15
更新日期:1997-06-01 00:00:00
abstract::Although analytical treatment interruption is used as a strategy to test immunotherapeutic agents in HIV-infection, it may pose a risk for study participants. The potential risks of short-term interruption of antiretroviral therapy (ART) during treatment with an autologous dendritic cell immune-based therapy (AGS-004-...
journal_title:Journal of medical virology
pub_type: 杂志文章,随机对照试验
doi:10.1002/jmv.23297
更新日期:2012-06-01 00:00:00
abstract::In this study, real-time PCR was used to quantify adenovirus DNA in cell suspensions prepared from 106 right and left tonsils and 10 adenoids obtained from 57 patients who underwent routine tonsillectomies and/or adenoidectomies. Eighty-four (72.4%) tonsils and adenoids samples were positive for HAdV by real-time PCR....
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23678
更新日期:2013-11-01 00:00:00
abstract::Nucleotide sequences of the hypervariable region (HVR) of the E2/NS1 gene of hepatitis C virus (HCV), which are now thought to contain epitopes for neutralizing antibodies, were compared between antibody-bound HCV and free HCV in a patient with type II cryoglobulinemia. Antibody-bound HCV was immunoprecipitated with a...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890460418
更新日期:1995-08-01 00:00:00
abstract::To determine the seroprevalence of antibodies against dengue virus (DENV) and West Nile virus (WNV) in the human population of the Bolivian Chaco, we tested 256 inhabitants of two rural communities. The seroprevalence, confirmed by plaque reduction neutralization test, was 7.8% and 2.7% for DENV and WNV, respectively....
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25283
更新日期:2019-01-01 00:00:00
abstract::Increased expression of inducible nitric oxide synthase (iNOS) has been found in inflammatory myocardial disease and increased production of nitric oxide (NO) has both an inhibitory effect on virus replication and a cytotoxic effect on host cells. To investigate the relationship between severity of enteroviral myocard...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1033
更新日期:2001-06-01 00:00:00
abstract::We report a male caucasian German pediatric patient of no Arab or Mediterranean ancestry with virus associated CNS lesions in Griscelli's syndrome (GS; McKusick No. 214450). The boy presented with recurrent infections, and meningitis with subsequent progressive signs of increased intracranial pressure leading to death...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(199711)53:3<306::aid-jmv21
更新日期:1997-11-01 00:00:00