Abstract:
:Physicochemical and molecular characterization studies of hepatitis C virus (HCV), the major causative agent of parenterally transmitted non-A, non-B hepatitis (PT-NANBH), strongly suggest that it is a pesti-/flavivirus-like virus. Additional studies show that the buoyant density of plasma-derived HCV in sucrose is significantly lower than that of most tissue culture-derived flaviviruses (1.20 g/cm3). Our finding suggests, but does not prove, that at least one physicochemical property of HCV is more similar to that of the pestiviruses, bovine viral diarrhea virus (BVDV) and hog cholera virus (HogCV), than that of the flaviviruses.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Bradley D,McCaustland K,Krawczynski K,Spelbring J,Humphrey C,Cook EHdoi
10.1002/jmv.1890340315keywords:
subject
Has Abstractpub_date
1991-07-01 00:00:00pages
206-8issue
3eissn
0146-6615issn
1096-9071journal_volume
34pub_type
杂志文章abstract::human diploid fibroblasts and human primary liver cell carcinoma cells (PLC/PRF/5) were infected with hepatitis A virus (HAV) adapted to growth in cell culture or derived directly from human stool. Viral antigen was expressed in PLC/PRF/5 cells 28 days after infection with cell culture-adapted HAV, and 50 days after i...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890070308
更新日期:1981-01-01 00:00:00
abstract::Rotaviruses are the major etiological agents of diarrhea in children less than 5 years of age. The commonest G types in humans are G1-4 and G9. G12 is a rare human rotavirus (HRV) strain first reported in the Philippines. In this study, 13 G12 strains obtained from a community-based cohort and a hospital-based surveil...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20872
更新日期:2007-09-01 00:00:00
abstract::High risk types of human papillomaviruses E6/E7 oncogenes and their association with tumor suppressor genes products are the key factors of cervical carcinogenesis. This study proposed them as specific markers for cervical dysplasia screening. The aim of the study is to compare the clinical and prognostic significance...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24199
更新日期:2015-09-01 00:00:00
abstract::Faeces from 746 children less than 5 years old with acute gastroenteritis were screened for the presence of adenovirus particles or antigens by immunoelectron microscopy (IEM) and enzyme immunoassay (EIA). Thirty-five samples were positive by both IEM and EIA, two only by IEM, and two only by EIA, giving a total of 39...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890150213
更新日期:1985-02-01 00:00:00
abstract::The long-term efficacy of lamivudine therapy in patients with hepatitis B virus (HBV) infection is still not clear. In this study, 20 non-cirrhotic Japanese patients infected with HBV received lamivudine therapy for more than 1 year and were followed for a median period of 8.5 years (range, 6.7-8.7 years). The rates o...
journal_title:Journal of medical virology
pub_type: 临床试验,杂志文章
doi:10.1002/jmv.20305
更新日期:2005-04-01 00:00:00
abstract::Hepatitis C virus (HCV) virions have been shown to be bound to antibodies in patients with chronic HCV infection. The sera from patients infected with genotype 1b HCV contained more antibody-free virions than those from patients with genotype 2a/2b HCV. When compared at the same levels of serum HCV RNA, free virion ti...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:2000-05-01 00:00:00
abstract::Hepatitis C Virus (HCV) NS3 protease is an attractive target for antiviral agent development because it is required for viral replication. Because a stable cell culture system or small animal model to study HCV replication is not readily available, we constructed an in vitro model allowing the investigation of NS3 tra...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.2089
更新日期:2001-12-01 00:00:00
abstract::Rotavirus vaccines have been available in Japan since 2011. This study conducted to monitor the trend of group A rotavirus (RVA) genotypes 3 years after vaccine introduction. A total of, 539 fecal samples were collected from children with acute gastroenteritis in six regions during July 2014-June 2015. Among them, 178...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25067
更新日期:2018-06-01 00:00:00
abstract::This study aimed to examine the application of hepatitis B surface antigen (HBsAg) confirmatory testing when diagnosing hepatitis B infection among young persons in Taiwan with a low prevalence rate of hepatitis B infection. HBsAg status, the presence of antibodies against HBsAg (anti-HBs), and the presence of antibod...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.22127
更新日期:2011-09-01 00:00:00
abstract::Rotavirus A (RVA) is the leading cause of acute viral gastroenteritis in children under 5 years of age worldwide. G9P[8] is a common RVA genotype that has been persistently prevalent in Jiangsu, China. To determine the genetic diversity of G9P[8] RVAs, 7 representative G9P[8] strains collected from Suzhou Children's H...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25214
更新日期:2018-09-01 00:00:00
abstract::There are contradictory reports regarding the effects of inhalation anesthetics on the immune system. Measurable immune responses have been studied in vitro, but little is known about the in vivo effects in the intact organism. We used an attenuated, non-neuroinvasive, nonlethal strain of the encephalitic West Nile vi...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.2184
更新日期:2002-04-01 00:00:00
abstract::Nasal washings were collected from 27 normal adults during 38 naturally acquired colds. The washings were exhaustively tested using tissue cultures, organ cultures and electron microscopy. Washings yielding no identifiable agent were inoculated into human volunteers, and further specimens obtained from the latter were...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890050306
更新日期:1980-01-01 00:00:00
abstract::Nasopharyngeal carcinoma is closely associated with Epstein-Barr virus (EBV) and the EBV encoded latent membrane protein-1 expression (LMP1) is commonly found in the tumour cells. LMP1 has been shown to be involved in modulation of cell growth in B cells but the biological properties of LMP1 expression in nasopharynge...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.2112
更新日期:2002-01-01 00:00:00
abstract::One hundred twelve peripheral blood specimens were tested for the presence of cytomegalovirus (CMV) by the tube culture indirect immunoperoxidase (TC-IPA) procedure, the shell vial assay [shell vials were pre- and postinoculation treated with medium containing 2 of 10% fetal bovine serum (FBS) or 100 micrograms% corti...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890380109
更新日期:1992-09-01 00:00:00
abstract::Human parvovirus B19 commonly infects children, causing erythema infectiosum (fifth disease). However, there is a significant adult population which has not been exposed to the virus and, consequently, does not have protective antibody. Recent reports have associated B19 infection during pregnancy with fetal death, al...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890230409
更新日期:1987-12-01 00:00:00
abstract::Five hundred and forty-seven pregnant women with less than 32 weeks of amenorrhoea, attending an antenatal clinic of St. Camille Medical Centre (SCMC) of Ouagadougou were enrolled for a hepatitis C virus (HCV) and HIV co-infection study. Fifty-eight (10.6%) were HIV positive and 18 (3.3%) were anti-HCV positive. Only ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20258
更新日期:2005-02-01 00:00:00
abstract::In mid-December 2019, a disease caused by infection with severe acute respiratory syndrome coronavirus-2, which began in Wuhan, China, has spread throughout the country and many countries around the world. The number of children with coronavirus disease-2019 (COVID-19) has also increased significantly. Although inform...
journal_title:Journal of medical virology
pub_type: 杂志文章,评审
doi:10.1002/jmv.25807
更新日期:2020-07-01 00:00:00
abstract::The prevalence of HCV genotypes in four republics of the former Soviet Union (Russia, Belarus, Moldova, and Uzbekistan) was investigated. Overall, 197 HCV isolates from 66 blood donors and 131 patients with chronic hepatitis were typed. Viral sequences from sera of infected subjects were amplified by nested RT-PCR usi...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:1997-09-01 00:00:00
abstract::Serial serum samples from 20 untreated patients with chronic hepatitis C virus (HCV) infection were tested for HCV RNA by a nested polymerase chain reaction assay using primers from the highly conserved 5' noncoding region to determine the relationship between hepatitis C viremia and the activity of liver disease duri...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890410415
更新日期:1993-12-01 00:00:00
abstract::To establish gut immunity in monkeys to polio-virus type 1, we fed four juvenile bonnet monkeys (Macaca radiata) with 100 median infectious doses of the Mahoney strain of virus. The duration of viral shedding in the throat and faeces and the titre of virus in faeces were measured. Eighteen days after the last monkey s...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890270208
更新日期:1989-02-01 00:00:00
abstract::Serial serum samples from 16 Italian patients presenting with acute hepatitis C virus (HCV) infections (which progressed to chronic hepatitis in six) were screened for the non-organ-specific autoantibodies most frequently associated with autoimmune hepatitis (AIH), as well as for antibodies against the hepatic asialog...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890420113
更新日期:1994-01-01 00:00:00
abstract::No controlled trial in patients with chronic hepatitis B virus (HBV) infection on long-term entecavir (ETV) treatment, comparing switching to tenofovir disoproxil fumarate (TDF) with continuing the therapy, has been reported. Twenty-seven nucleos(t)ide-naïve patients with chronic HBV who underwent ETV therapy for ≥5 y...
journal_title:Journal of medical virology
pub_type: 杂志文章,随机对照试验
doi:10.1002/jmv.25442
更新日期:2019-07-01 00:00:00
abstract::Human herpesvirus 8 (HHV-8) is the etiologic agent of Kaposi's sarcoma (KS). Several studies indicate horizontal HHV-8 transmission among children in areas where KS is endemic, but few studies have assessed acquisition of HHV-8 by children in low seroprevalence areas. Antibody screening was carried out for HHV-8 and E...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10548
更新日期:2004-01-01 00:00:00
abstract::Five methods were evaluated for the detection of adenovirus directly from nasopharyngeal aspirates (NPA), including conventional and rapid virus culture, two antigen detection tests, and the polymerase chain reaction (PCR). NPA specimens were obtained from 269 military conscripts suffering from an acute respiratory in...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:1999-09-01 00:00:00
abstract::In earlier studies, hepatitis E virus (HEV) particles were detected in the stools of patients with enterically transmitted non-A, non-B (ENANB) hepatitis, and HEV was etiologically associated with this disease. Such particles have not been observed in the liver, however. We describe the pathological findings in the li...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890310310
更新日期:1990-07-01 00:00:00
abstract::Glycoproteins of enveloped viruses replicating in nonprimate mammalian cells carry α-1,3-galactose (α-Gal) glycans, and can bind to anti-Gal antibodies which are abundant in humans. The antibodies have protected humans and their ancestors for millions of years, because they inhibit replication of many kinds of microbe...
journal_title:Journal of medical virology
pub_type: 杂志文章,评审
doi:10.1002/jmv.26312
更新日期:2020-07-18 00:00:00
abstract::There are limited reports on the etiology of multiple enteric viruses causing acute gastroenteritis (AGE) in North India. In the present study we have determined the prevalence of three enteric viruses, namely rotavirus, astrovirus (AstV) and adenovirus (AdV) in a total of 312 diarrheic children (<5 years) hospitalize...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25645
更新日期:2020-08-01 00:00:00
abstract::This is an audit of a new technique to improve the colposcopy service. Samples were tested for high risk HPV DNA using Digene Hybrid Capture II. Sixty-four percent of the sampled women under 30 had detectable high risk HPV DNA, decreasing to 44% in 30--39 year olds and to 27% in women over 40. High risk HPV prevalence...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20401
更新日期:2005-08-01 00:00:00
abstract::Capillary blood of febrile children was lysed by using a lysis buffer containing ascorbic acid. MxA quantitation was performed by an immunochemiluminescent assay. The MxA values were significantly higher in capillary blood of infants with viral infections due to adenovirus (n = 5), rotavirus (n = 15), or respiratory s...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract::A real-time PCR assay was developed for quantitative detection of B19 DNA in clinical serum samples. The assay was carried out using a LightCycler instrument and product formation was monitored continuously with the fluorescent double-stranded DNA binding dye SYBR Green I. With an optimized PCR protocol, this system w...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.2218
更新日期:2002-06-01 00:00:00