Adenoviruses in faeces of children with acute gastroenteritis in Rio de Janeiro, Brazil.

Abstract:

:Faeces from 746 children less than 5 years old with acute gastroenteritis were screened for the presence of adenovirus particles or antigens by immunoelectron microscopy (IEM) and enzyme immunoassay (EIA). Thirty-five samples were positive by both IEM and EIA, two only by IEM, and two only by EIA, giving a total of 39 (5.2%) samples with positive results. Of these, 25 could be propagated in HEp2 cells and were neutralized by one of the antisera to adenovirus types 1 to 18. The remaining 14 samples could be propagated only in the 293 permanent line of human cells transformed by adenovirus type 5 DNA [Graham et al, 1977] and were not neutralized by antisera to adenovirus types 1 to 31. An EIA carried out by the antibody-capture technique, using antiserum specific for "enteric" adenoviruses [Johansson et al, 1979], gave positive results with all isolates that could be propagated only in 293 cells and with none of those capable of growing in HEp2 cells.

journal_name

J Med Virol

authors

Leite JP,Pereira HG,Azeredo RS,Schatzmayr HG

doi

10.1002/jmv.1890150213

subject

Has Abstract

pub_date

1985-02-01 00:00:00

pages

203-9

issue

2

eissn

0146-6615

issn

1096-9071

journal_volume

15

pub_type

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