Propagation of hepatitis A virus in human embryo fibroblasts.

Abstract:

:human diploid fibroblasts and human primary liver cell carcinoma cells (PLC/PRF/5) were infected with hepatitis A virus (HAV) adapted to growth in cell culture or derived directly from human stool. Viral antigen was expressed in PLC/PRF/5 cells 28 days after infection with cell culture-adapted HAV, and 50 days after infection with virus from human stool. In human fibroblasts the periods until first expression of viral antigen were 90 and 210 days, respectively. During further passages of HAV in fibroblasts the time of first appearance of antigen decreased to about 28 days. Biophysical properties of HAV extracted from infected fibroblasts were comparable to those of HAV derived directly from human stool. Immunofluorescence studies showed that the antigen was located exclusively within the cytoplasm of the infected fibroblasts. Kinetics of antigen production indicated that an equilibrium between virus multiplication and cell metabolism was reached in persistently infected fibroblasts.

journal_name

J Med Virol

authors

Gauss-Müller V,Frösner GG,Deinhardt F

doi

10.1002/jmv.1890070308

subject

Has Abstract

pub_date

1981-01-01 00:00:00

pages

233-9

issue

3

eissn

0146-6615

issn

1096-9071

journal_volume

7

pub_type

杂志文章
  • Contribution of common and recently described respiratory viruses to annual hospitalizations in children in South Africa.

    abstract::The contribution of viruses to lower respiratory tract disease in sub-Saharan Africa where human immunodeficiency virus may exacerbate respiratory infections is not well defined. No data exist on some of these viruses for Southern Africa. Comprehensive molecular screening may define the role of these viruses as single...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.22120

    authors: Venter M,Lassaunière R,Kresfelder TL,Westerberg Y,Visser A

    更新日期:2011-08-01 00:00:00

  • Association between detectable SARS-COV-2 RNA in anal swabs and disease severity in patients with coronavirus disease 2019.

    abstract:BACKGROUND:Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA was found in the intestines and feces, but its clinical significance is not completely clear. We aim to characterize the longitudinal test results of SARS-CoV-2 RNA in anal swabs and to explore the association with disease severity. METHODS:We...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.26307

    authors: Lin W,Xie Z,Li Y,Li L,Wen C,Cao Y,Chen X,Ou X,Hu F,Li F,Tang X,Cai W,Li L

    更新日期:2021-02-01 00:00:00

  • Prevalence of antibodies to enteroviruses and varicella-zoster virus among residents and overseas volunteers at agricultural settlements in Israel.

    abstract::Within the framework of a comprehensive study of the correlation between enteric diseases and wastewater utilization in agricultural settlements (kibbutzim) the prevalence of several viral antibodies was examined among kibbutz residents and overseas volunteers. The latter were assumed to be a group highly susceptible ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890200211

    authors: Margalith M,Fattal B,Shuval HI,Morag A

    更新日期:1986-10-01 00:00:00

  • Removal of parvovirus B19 from contaminated factor VIII during fractionation.

    abstract::A solution of pooled cryo-precipitate for preparing factor VIII (F VIII) by the solvent/detergent method was contaminated experimentally with parvovirus B19-positive plasma to evaluate virus reduction achieved by the final steps of the F VIII production process. Virus reduction was at least 2 logs of the total amount ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890350107

    authors: Schwarz TF,Roggendorf M,Hottenträger B,Stolz W,Schwinn H

    更新日期:1991-09-01 00:00:00

  • A variable region in the Crimean-Congo hemorrhagic fever virus L segment distinguishes between strains isolated from different geographic regions.

    abstract::Alignment of Crimean-Congo hemorrhagic fever virus (CCHFV) L genome segment full-length sequences reveals an overall high level of conservation among strains, with greater than 90% of translated amino acid residues strictly conserved. However, a region of marked variability identified previously, corresponding to L po...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20531

    authors: Meissner JD,Seregin SS,Seregin SV,Vyshemirskii OI,Samokhvalov EI,Lvov DK,Netesov SV,Petrov VS

    更新日期:2006-02-01 00:00:00

  • Seroprevalence of six different viruses among pregnant women and blood donors in rural and urban Burkina Faso: A comparative analysis.

    abstract::A seroprevalence study was carried out of six different human pathogenic viruses, namely human immunodeficiency virus (HIV), hepatitis B virus (HBV), hepatitis C virus (HCV), human T-cell leukemia virus (HTLV), human herpesvirus type 8 (HHV-8), and dengue virus among pregnant women and blood donors from rural (Nouna) ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20593

    authors: Collenberg E,Ouedraogo T,Ganamé J,Fickenscher H,Kynast-Wolf G,Becher H,Kouyaté B,Kräusslich HG,Sangaré L,Tebit DM

    更新日期:2006-05-01 00:00:00

  • Identification of a human group C rotavirus in Malaysia.

    abstract::Stool specimens from 334 infants and young children hospitalized with diarrhea in the General Hospital, Kuala Lumpur, Malaysia between August and November, 1987 were analyzed for the presence of rotavirus double-stranded (ds) RNA by polyacrylamide gel electrophoresis. Of the 334 specimens analyzed, 32 (9.6%) were posi...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890430302

    authors: Rasool NB,Hamzah M,Jegathesan M,Wong YH,Qian Y,Green KY

    更新日期:1994-07-01 00:00:00

  • Hepatitis B control in Europe by universal vaccination programmes: the situation in 2001.

    abstract::In the nine years since the Global Advisory Group of the Expanded Programme on Immunisation (WHO) set 1997 as the target for integrating hepatitis B vaccination into national immunisation programmes worldwide, 129 countries have included hepatitis B vaccine as part of their routine infant or adolescent immunisation pr...

    journal_title:Journal of medical virology

    pub_type: 杂志文章,评审

    doi:10.1002/jmv.10091

    authors: Van Damme P,Vorsters A

    更新日期:2002-07-01 00:00:00

  • Immunogenicity of wild and attenuated varicella-zoster virus strains in rhesus monkeys.

    abstract::Thirty susceptible rhesus monkeys were inoculated with cell-free varicella-zoster virus strain OKA or strain KMcC. Both wild and attenuated strains were used. No clinical signs characteristic of human varicella were seen in any of the animals. Virus was not isolated from throat swabs, blood, or cerebrospinal fluid. An...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890140403

    authors: Asano Y,Albrecht P,Behr DE,Neff BJ,Vickers JH,Rastogi SC

    更新日期:1984-01-01 00:00:00

  • Human herpesvirus-8 infection in pregnancy and labor: lack of evidence of vertical transmission.

    abstract::To investigate whether vertical transmission of the human herpesvirus 8 (HHV-8) may occur during pregnancy or at delivery, we enrolled 295 women recruited attending the Division of Obstetrics and Gynecology of a University Teaching of Rome Tor Vergata, S. Eugenio Hospital. The study population was divided in two group...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20012

    authors: Sarmati L,Carlo T,Rossella S,Montano M,Adalgisa P,Rezza G,Andreoni M

    更新日期:2004-03-01 00:00:00

  • Junin virus-induced astrocytosis is impaired by iNOS inhibition.

    abstract::Because Junin virus (JV) experimental encephalitis of mice and rats is characterized by mild histopathological changes that do not seem to justify per se lethality after intracerebral infection, such a murine model seems adequate to investigate the potential role of inducible nitric oxide synthase (iNOS) as a pathogen...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.10254

    authors: Gómez RM,Yep A,Schattner M,Berría MI

    更新日期:2003-01-01 00:00:00

  • Algorithm based on CMV kinetics DNA viral load for preemptive therapy initiation after hematopoietic cell transplantation.

    abstract::Preemptive therapy in hematopoietic cell transplantation is initiated by a diagnostic technique at first detection of cytomegalovirus (CMV). The aim of this study was to use the viral dynamics of CMV DNA viral to start preemptive therapy, and to prospectively compare the CMV viral load kinetics to pp65 antigenemia. Tw...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21996

    authors: Halfon P,Berger P,Khiri H,Martineau A,Pénaranda G,Merlin M,Faucher C

    更新日期:2011-03-01 00:00:00

  • Measles virus genotype B2 is not inactive: evidence of continued circulation in Africa.

    abstract::This study describes two measles outbreaks--one in Cape Town, South Africa in November 2002, and the other in Luanda, Angola in March 2003. The outbreaks were notable because they were caused by closely related genotype B2 viruses. This genotype was first described in an outbreak in Libreville, Gabon in the 1980s and ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20491

    authors: Smit SB,Hardie D,Tiemessen CT

    更新日期:2005-12-01 00:00:00

  • Serum thrombospondin-1 is altered in patients with hemorrhagic fever with renal syndrome.

    abstract::Hemorrhagic fever with renal syndrome (HFRS) is a severe acute viral disease with pathological changes of impaired capillary and small vessels and thrombocytopenia. In this study, serum thrombospondin (TSP)-1 concentration in patients with HFRS was determined to explore its possible role in the pathogenesis of HFRS. T...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21270

    authors: Liu Z,Zhao Q,Han Q,Gao M,Zhang N

    更新日期:2008-10-01 00:00:00

  • Replication-defective mutants of mouse cytomegalovirus protect against wild-type virus challenge.

    abstract::Five temperature-sensitive mutants (tsm9, tsm13, tsm20, tsm22, tsm30) of murine cytomegalovirus have been shown previously not to produce infectious virus in mice. In the present study, the stage at which these mutants are blocked in their replication in vitro was examined by transcriptional analysis of 4 temporally r...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/1096-9071(200010)62:2<127::aid-jmv2>3.0.co

    authors: Gill TA,Morley PJ,Sweet C

    更新日期:2000-10-01 00:00:00

  • Variation in enterovirus receptor genes.

    abstract::The increased incidence of a enterovirus infections observed in patients with type 1 diabetes preceding the development of the clinical disease could be partially explained by variation in the genes coding for enterovirus receptors. We carried out sequence analysis of the most common enterovirus receptor molecules in ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.10352

    authors: Karttunen A,Pöyry T,Vaarala O,Ilonen J,Hovi T,Roivainen M,Hyypiä T

    更新日期:2003-05-01 00:00:00

  • Antibody assays for varicella-zoster virus: comparison of competitive enzyme-linked immunosorbent assay (ELISA), competitive radioimmunoassay (RIA), complement fixation, and indirect immunofluorescence assays.

    abstract::The competitive ELISA test was compared with competitive RIA, complement fixation, and indirect fluorescent antibody test for the detection of antibody to varicella-zoster virus (VZV). ELISA and RIA were the most sensitive tests, being tenfold as sensitive as indirect immunofluorescence and 20 times as sensitive as co...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890130407

    authors: Wreghitt TG,Tedder RS,Nagington J,Ferns RB

    更新日期:1984-01-01 00:00:00

  • Association between HLA-DRB1 polymorphism, high-risk HPV infection and cervical neoplasia in southern Chinese.

    abstract::Multiple determinants are involved in the progression of human papillomavirus (HPV)-infected cervical lesion to invasive cancer. Human leukocyte antigen (HLA) polymorphism seems to play a role. This study examined the association between HLA-DRB1 polymorphism, high-risk HPV infection, and the development of cervical n...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20805

    authors: Chan PK,Cheung TH,Lin CK,Siu SS,Yim SF,Lo KW,Cheung JL,Tam AO,Tang JW

    更新日期:2007-07-01 00:00:00

  • Phylogenetic analysis of hepatitis C virus strains and risk factors associated with infection and viral subtypes among Iranian patients.

    abstract::Hepatitis C virus (HCV) has infected approximately 170 million people worldwide. While the seroprevalence of anti-HCV antibody among Iranian blood donors is 0.13%, HCV infection is prevalent in 59-80% of Iranian injecting drug users. One hundred seventy-eight anti-HCV positive patients were referred to the Gastroenter...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.23947

    authors: Salehi Moghadam F,Mohebbi SR,Hosseini SM,Romani S,Mirtalebi H,Azimzadeh P,Damavand B,Naghoosi H,Khanyaghma M,Sanati A,Zali MR

    更新日期:2014-08-01 00:00:00

  • Herd immunity and fatal cases of influenza among the population exposed to poultry and wild birds in Russian Asia in 2013-2014.

    abstract::In total 1,525 blood serum samples were collected in October, 2013 in Russian Asia from people who reside in territories that are at high risk for emergence of influenza viruses with pandemic potential. Presence of antibodies to influenza viruses in the sera was tested in hemagglutination inhibition test. None of the ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.24301

    authors: Ilyicheva T,Abdurashitov M,Durymanov A,Susloparov I,Goncharova N,Kolosova N,Mikheev V,Ryzhikov A

    更新日期:2016-01-01 00:00:00

  • Quantification of infectious duck hepatitis B virus by radioimmunofocus assay.

    abstract::A simple method is described for the precise quantification of infectious duck hepatitis B virus (DHBV) in cell culture, using a radioimmunofocus assay (RIFA). Primary duck hepatocyte cell cultures were infected with serial dilutions of viral samples as for a plaque assay, but then maintained with liquid overlay mediu...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:

    authors: Anderson DA,Grgacic EV,Luscombe CA,Gu X,Dixon R

    更新日期:1997-08-01 00:00:00

  • Occurrence rate and genotype distribution of the JC virus (JCV) in a sample from the Polish population.

    abstract::The JC virus (JCV) is a common human virus persisting in renal tissue. In immunocompromised individuals it may reactivate and cause progressive multifocal leukoencephalopathy (PML). JCV has also been implicated in cancerogenesis, leading to various brain tumors and cancers of gastrointestinal tract. In this study the ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21153

    authors: Kmieciak D,Debicki S,Trzeciak WH

    更新日期:2008-06-01 00:00:00

  • Heterogeneity in E2 region of GBV-C/hepatitis G virus and hepatitis C virus.

    abstract::GB virus C/hepatitis G virus (GBV-C/HGV) is related distantly to hepatitis C virus (HCV). HCV has a hypervariable region (HVR), and exists as quasispecies in vivo. Although GBV-C/HGV also has replaceable amino acids in the presumed antigenic region, the existence and fluctuation of population of heterogeneous virus ha...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:

    authors: Kato T,Mizokami M,Nakano T,Orito E,Ohba K,Kondo Y,Tanaka Y,Ueda R,Mukaide M,Fujita K,Yasuda K,Iino S

    更新日期:1998-06-01 00:00:00

  • Incident and long-term HIV-1 infection among pregnant women in Brazil: Transmitted drug resistance and mother-to-child transmission.

    abstract::Primary infection, seroconversion, and transmitted drug resistance (TDR) during pregnancy may influence the risk of mother-to-child-transmission (MTCT) of HIV-1 infection. This study estimated recent seroconversion, TDR rates, HIV-1 subtypes and pregnancy outcomes among 95 recently diagnosed, antiretroviral (ARV)-naïv...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.24540

    authors: Lima YA,Cardoso LP,Reis MN,Stefani MM

    更新日期:2016-11-01 00:00:00

  • Identification and characterization of enteric adenoviruses in infants and children hospitalized for acute gastroenteritis.

    abstract::Enteric adenoviruses are important etiological agents associated with sporadic infections and outbreaks of acute gastroenteritis in infants and children. Fecal samples were collected from 439 hospitalized patients in the years 2005-2007 from Pune, Aurangabad, and Nagpur cities of western India to identify the most pre...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21331

    authors: Verma H,Chitambar SD,Varanasi G

    更新日期:2009-01-01 00:00:00

  • Prevalence of delta infection in the western Pacific region.

    abstract::The prevalence of coinfection and superinfection with the delta agent was studied in 2,645 hepatitis B surface antigen (HBsAg)-positive subjects from six countries and nine islands in the Western Pacific region. The study group comprised 262 patients with acute hepatitis B and 2,383 chronic carriers of HBsAg, of whom ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890180406

    authors: Dimitrakakis M,Crowe S,Gust I

    更新日期:1986-04-01 00:00:00

  • Epidemiology and clinical features of rotavirus, adenovirus, and astrovirus infections and coinfections in children with acute gastroenteritis prior to rotavirus vaccine introduction in Meerut, North India.

    abstract::There are limited reports on the etiology of multiple enteric viruses causing acute gastroenteritis (AGE) in North India. In the present study we have determined the prevalence of three enteric viruses, namely rotavirus, astrovirus (AstV) and adenovirus (AdV) in a total of 312 diarrheic children (<5 years) hospitalize...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.25645

    authors: Akdag AI,Gupta S,Khan N,Upadhayay A,Ray P

    更新日期:2020-08-01 00:00:00

  • Recent trends in hepatitis A incidence in Brazil.

    abstract::Hepatitis A incidence has been decreasing in Brazil since child vaccination was implemented in 2014. This trend was interrupted by an outbreak among adult male in São Paulo in 2017. This study was outlined to estimate whether the increase of hepatitis A cases among adult men in Brazil was restricted to São Paulo. Case...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.25694

    authors: De Oliveira TM,Vieira NSG,Sepp TDS,Souto FJD

    更新日期:2020-08-01 00:00:00

  • Foscarnet therapy in chronic hepatitis B virus E antigen carriers.

    abstract::Foscarnet (trisodium phosphonoformate) is a novel antiviral agent that inhibits viral-specific DNA polymerase. In the present study, eight males with chronic HBV carriage (HBeAg and HBV-DNA seropositivity greater than 12 months) showing chronic persistent hepatitis (CPH) or chronic active hepatitis (CAH) on liver biop...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890290214

    authors: Bain VG,Daniels HM,Chanas A,Alexander GJ,Williams R

    更新日期:1989-10-01 00:00:00

  • High prevalence of non-B HIV-1 subtypes in seamen and their sexual partners in Croatia.

    abstract::Travel influences the spread of non-B subtypes of HIV in Europe. The aim of this study was to assess whether the spread of non-B subtypes occurred in Croatia, a country with a low level HIV infection. Phylogenetic analysis of the reverse transcriptase and protease genes was undertaken in 145 Croatian patients with the...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21433

    authors: Ramirez-Piedad MK,Lepej SZ,Yerly S,Begovac J

    更新日期:2009-04-01 00:00:00