Abstract:
:The competitive ELISA test was compared with competitive RIA, complement fixation, and indirect fluorescent antibody test for the detection of antibody to varicella-zoster virus (VZV). ELISA and RIA were the most sensitive tests, being tenfold as sensitive as indirect immunofluorescence and 20 times as sensitive as complement fixation, which was surprisingly inadequate for detecting anti-VZV in sera from regular haemodialysis patients. The ELISA test was found to be the most suitable assay, employing a routine complement fixation test (CFT) grade viral antigen and not requiring radiolabelling and counting facilities, which are unavailable in most virology laboratories.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Wreghitt TG,Tedder RS,Nagington J,Ferns RBdoi
10.1002/jmv.1890130407subject
Has Abstractpub_date
1984-01-01 00:00:00pages
361-70issue
4eissn
0146-6615issn
1096-9071journal_volume
13pub_type
杂志文章abstract::Genotype-specific effects of parvovirus B19 (B19V) infections on left ventricular function in patients with dilated cardiomyopathy (DCM) have not been investigated so far. In this prospective clinical study, the prevalences of B19V genotypes in endomyocardial biopsies from patients presenting with inflammatory heart d...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.22187
更新日期:2011-10-01 00:00:00
abstract::Human parainfluenza virus 3 (HPIV-3) is a leading cause of respiratory disease in children worldwide. Previous sequence analyses of the entire virus genome, among different HPIV-3 strains, demonstrated that HN is the most variable gene. There is a dearth of data on HPIV-3 strains circulating in Saudi Arabia. In this r...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23326
更新日期:2012-08-01 00:00:00
abstract::We previously reported an age-associated impairment of serum immunoglobulin G (IgG) antibody responses to inactivated influenza virus vaccine. The present study extends these observations by examining the IgG subclass distribution of vaccine responses measured by enzyme linked immunosorbent assay in healthy adults age...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890430111
更新日期:1994-05-01 00:00:00
abstract::Corticosteroid use in transplant recipients increases the incidence and severity of Kaposi's sarcoma (KS), a disease associated with KS-associated herpesvirus (KSHV) infection. Recently, the prototypic corticosteroid, hydrocortisone, was shown to directly induce lytic cycle reactivation of KSHV in latently-infected BC...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.2155
更新日期:2002-03-01 00:00:00
abstract::Human peripheral blood mononuclear cell (PBMC) proliferative responses to live respiratory syncytial (RS) virus, formalin-inactivated RS (Fl-RS) virus, RS virus F (fusion) protein, and RS virus G (attachment) protein were assessed. All donors responded to challenge with whole RS virus antigens and F and G proteins. F ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(SICI)1096-9071(199607)49:3<161::AID-JMV2>
更新日期:1996-07-01 00:00:00
abstract::Here, we studied the role of nitric oxide (NO) production during the first steps of the respiratory infection of BALB/c mice with herpes simplex virus type 1 (HSV-1), strain F. Nitric oxide synthase II (NOS-II) mRNA and protein were detected by reverse transcription (RT)-PCR and dot blot, respectively in samples of lu...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20093
更新日期:2004-06-01 00:00:00
abstract::Oral DAA have demonstrated high efficacy as treatment of hepatitis C. However, the presence of resistance-associated substitutions (RAS) at baseline has occasionally been associated with impaired treatment response. Herein, we examined the impact of baseline RAS at the HCV NS5A gene region on treatment response in a r...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24971
更新日期:2018-03-01 00:00:00
abstract::Recruitment of leukocytes by chemokines and chemokine receptors to CNS plays a crucial role in the induction of inflammatory response in HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). In the present study, chemokine and chemokine receptors involved in trafficking of lymphocytes to the CNS were me...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24779
更新日期:2017-08-01 00:00:00
abstract::The prognostic value of early hepatitis C virus (HCV)-RNA load was evaluated among nonresponder patients to previous interferon (IFN) therapy treated with daily IFN and ribavirin. One hundred-six nonresponders (83 men), mean age 44.8 +/- 11 years, were treated with IFN-alpha 2b 3 MU/day for 24 weeks, followed by 3 MU ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10528
更新日期:2004-01-01 00:00:00
abstract::Nasopharyngeal carcinoma is closely associated with Epstein-Barr virus (EBV) and the EBV encoded latent membrane protein-1 expression (LMP1) is commonly found in the tumour cells. LMP1 has been shown to be involved in modulation of cell growth in B cells but the biological properties of LMP1 expression in nasopharynge...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.2112
更新日期:2002-01-01 00:00:00
abstract::The mechanisms underlying chronicity of hepatitis C virus (HCV) infection are poorly understood, but the importance of impaired viral clearance by the immune system has been suggested. The prevalence of HCV infection of peripheral blood mononuclear cells (PBMC) was in investigated in 34 persistently infected patients ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890470112
更新日期:1995-09-01 00:00:00
abstract::Influenza epidemic is an important cause of severe illness in the elderly. Age-dependent morbidity of influenza in the elderly is associated with weakened immunity. The baseline age-related memory T cell activity in Chinese persons who are exposed to influenza virus through natural infection, are associated with the p...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21544
更新日期:2009-08-01 00:00:00
abstract::The effect of antiviral treatment on apoptosis in chronic hepatitis B (CHB) has not been clarified. We evaluated the hepatic immunohistochemical expression of the pro-apoptotic bax and the antiapoptotic bcl-xL protein in HBeAg-negative CHB patients before and after treatment. In our study we included 72 paired biopsie...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21780
更新日期:2011-07-01 00:00:00
abstract::The TT virus (TTV) was isolated recently from the serum of a patient with post-transfusion hepatitis. TTV infection is widespread in the general population, and its prevalence increases continuously with age. The pathogenic role of TTV in liver disease remains controversial, and the source of transmission is still unc...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20204
更新日期:2004-11-01 00:00:00
abstract::Primary infection, seroconversion, and transmitted drug resistance (TDR) during pregnancy may influence the risk of mother-to-child-transmission (MTCT) of HIV-1 infection. This study estimated recent seroconversion, TDR rates, HIV-1 subtypes and pregnancy outcomes among 95 recently diagnosed, antiretroviral (ARV)-naïv...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24540
更新日期:2016-11-01 00:00:00
abstract::Rapid PCR-based influenza tests are increasingly used as point-of-care diagnostics in hospitals and clinics. To our knowledge, no prior studies have described clinical outcomes with implementation of rapid PCR-based influenza tests in hospitalized adult inpatients. Electronic medical records were used to assess differ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24279
更新日期:2015-12-01 00:00:00
abstract::Human immunodeficiency virus 2 (HIV-2) infection is characterized by a slower disease progression and lower transmission rates. The molecular features that could be assigned as directly involved in this in vivo phenotype remain essentially unknown, and the importance of HIV-2 as a model to understand pathogenicity of ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21619
更新日期:2009-11-01 00:00:00
abstract::Norovirus is one of the major causes of outbreaks and sporadic cases of acute gastroenteritis in young children worldwide. Obtaining local baseline information regarding this virus is important for developing and evaluating prevention strategies of norovirus transmission in children. The age, seasonal distribution and...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21569
更新日期:2009-10-01 00:00:00
abstract::Human immunodeficiency virus (HIV) is the primary etiological agent of acquired immunodeficiency syndrome (AIDS). This virus can be transmitted by blood or blood products. Homosexual males (HM) are regarded as one of the high-risk groups for AIDS. Lymphocytes obtained from HM were used for cocultivation with fresh HIV...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890230404
更新日期:1987-12-01 00:00:00
abstract::The quantitation of serum levels of hepatitis C virus (HCV) RNA in chronic hepatitis C has been regarded as one of the most important indicators for the outcome of interferon (IFN) therapy. The AMPLICOR HCV MONITOR version 1.0 (AMPLICOR v1.0) assay is widely used for the evaluation of the HCV level. A new generation a...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10209
更新日期:2002-11-01 00:00:00
abstract::Many of the small round human fecal viruses implicated in outbreaks of nonbacterial gastroenteritis have been collected together and examined under the electron microscope. Negatively stained preparations without the addition of antibody were used so that the surface morphology of the virus particles remained clearly ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890090403
更新日期:1982-01-01 00:00:00
abstract::To assess whether the administration of a booster dose of influenza vaccine may enhance immune response in hemodialysis patients, 58 subjects were given two doses of the 2003/2004 season influenza vaccine, 1 month apart. "European Agency for the Evaluation of Medicinal Products" (EMEA) criteria were fully met in terms...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20936
更新日期:2007-08-01 00:00:00
abstract::Several viruses are associated with gastroenteritis in infants. This pilot study, nested within a larger community-based project of early childhood infections, collected daily symptom data and 511 weekly stool samples from five healthy, fully vaccinated, term infants from birth until their second birthday. Real-time P...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24716
更新日期:2017-05-01 00:00:00
abstract::Exposure to Ljungan virus (LV) is implicated in the risk of autoimmune (type 1) diabetes but possible contribution by other parechoviruses is not ruled out. The aim was to compare children diagnosed with type 1 diabetes in 2005-2011 (n = 69) with healthy controls (n = 294), all from the Jämtland County in Sweden, usin...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24127
更新日期:2015-07-01 00:00:00
abstract::The presence of HPV 16 E6*I/E6*II spliced transcripts, in cervical lesions of different grade, was analyzed to characterize the transcription pattern. The presence and amount of spliced transcripts were correlated with DNA viral markers such as E2/E6 ratio and physical state. The detection of HPV 16 E6*I/E6*II mRNAs w...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21496
更新日期:2009-07-01 00:00:00
abstract::Acute hepatitis B virus (HBV) infection was diagnosed in 57 adults admitted to Toranomon Hospital in Tokyo, Japan. Genotypes of HBV were determined by a serological method and compared to those in 1,077 patients with chronic hepatitis B. The distribution of genotypes were: genotype A (acute, 22.8% vs. chronic, 1.9%; P...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10239
更新日期:2002-12-01 00:00:00
abstract::The prevalence of picobirnaviruses (PBVs) in human stools was investigated by polyacrylamide gel electrophoresis (PAGE) analysis of 832 faecal specimens collected between 1982 and 1993 from patients in various clinical groups. Similar prevalences (9-13%) were detected in patients with or without gastroenteritis and th...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890450204
更新日期:1995-02-01 00:00:00
abstract::Physicochemical and molecular characterization studies of hepatitis C virus (HCV), the major causative agent of parenterally transmitted non-A, non-B hepatitis (PT-NANBH), strongly suggest that it is a pesti-/flavivirus-like virus. Additional studies show that the buoyant density of plasma-derived HCV in sucrose is si...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890340315
更新日期:1991-07-01 00:00:00
abstract::No data on antiviral response of HBV genotypes E-H are available so far although these HBV genotypes contribute significantly to the global HBV burden. Of 49 patients with HBV genotypes E-H, 23 received interferon (IFN)-alpha, 12 nucleos(t)ide analogues and 14 patients were untreated. HBV genotype was determined by di...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21588
更新日期:2009-10-01 00:00:00
abstract::Hepatitis C virus (HCV) represents a major cause of posttransfusion hepatitis worldwide. Posttransfusion hepatitis associated with hepatitis B virus (HBV) continues to occur. HBsAg-negative donor sera from the Rhode Island Blood Center between 1987 and 1988 were screened using more sensitive techniques to assess the p...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890420210
更新日期:1994-02-01 00:00:00