Abstract:
:Attachment of hepatitis B virus to a hepatoblastoma cell line (HepG2) was examined using a synthetic peptide corresponding to the pre-S1 (21-47) region of the envelope protein. Scatchard analysis revealed a single class binding site of Kd 104 +/- 27 nM/l and 5.4 +/- 1.2 x 10(5) sites per cell. Competition of HBV with pre-S1 peptides was dose dependent, and demonstrated it as the dominant binding site. In view of the suggested sequence homology between the peptide and IgA, cross-competition studies were carried out. The results indicate no direct role of IgA receptor in HBV binding. The receptor for the pre-S1 peptide was identified as a single major peptide of molecular weight 31 kD using in-situ ligand receptor crosslinking.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Dash S,Rao KV,Panda SKdoi
10.1002/jmv.1890370208keywords:
subject
Has Abstractpub_date
1992-06-01 00:00:00pages
116-21issue
2eissn
0146-6615issn
1096-9071journal_volume
37pub_type
杂志文章abstract::The prevention of mother to child transmission of HIV-1 by zidovudine monotherapy is well known, but increasingly combination anti-retroviral therapy is prescribed during pregnancy. In this prospective study, 19 pregnant women with human immunodeficiency virus-1 (HIV-1) infection who elected to take anti-retroviral th...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(199911)59:3<364::aid-jmv17
更新日期:1999-11-01 00:00:00
abstract::A modified centrifugation culture technique and a polymerase chain reaction (PCR) is described for detection of early antigen and IE antigen DNA, respectively, for rapid and sensitive monitoring of active cytomegalovirus (HCMV) infection after organ transplantation. In a preliminary study, 541 clinical specimens (bloo...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890380404
更新日期:1992-12-01 00:00:00
abstract::The seroprevalence of hepatitis A antibodies in travellers attending London Travel Clinics increases with age and screening may eliminate the need for vaccination at present for approximately 40% of adults. The duration of protection by current hepatitis A vaccine(s) is still to be established. ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890440414
更新日期:1994-12-01 00:00:00
abstract::To elucidate the relationship between the clinical severity of chronic liver disease and the precore mutations in hepatitis B e antigen (HBeAg)-negative hepatitis B virus (HBV) carriers, mutations were investigated in the precore region of HBV DNA in 20 chronic hepatitis B patients who seroconverted either spontaneous...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890450404
更新日期:1995-04-01 00:00:00
abstract::Analysis of 505 cases history of patients among men with viral hepatitis demonstrates that HBV infected patients represent 68.9% of the total and that a non-parenteral rate of transmission is the most likely means of hepatitis B infection. Saliva and serum testing for the presence of specific HBV markers (HBsAg, HBeAg...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/1096-9071(200008)61:4<433::aid-jmv4>3.0.co
更新日期:2000-08-01 00:00:00
abstract::Using immunodiffusion, a major cross-reactivity had been previously demonstrated between hepatitis B(HBe/3 Ag) and the antigen reported in the serum on non A, non-B hepatitis patients, therefore redesignated NANBe Ag. By direct immunofluorescence a new antigen associated with, but distinct from, NANBe Ag has now been ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890080103
更新日期:1981-01-01 00:00:00
abstract::Human herpes virus type 6 (HHV-6) DNA has been suggested to be a cofactor to human papillomavirus (HPV) in cervical cancer. In a cross-sectional study, we investigated the association between HHV-6 DNA detected in cervical brushings and high-grade squamous intraepithelial lesions (HSIL), while controlling for genital ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10229
更新日期:2002-12-01 00:00:00
abstract::Intramuscular (IM) influenza vaccines are about 50% effective in preventing clinical illness among the elderly and their effectiveness in eliciting mucosal response may be even lower. The aim of the present study was to evaluate the immunological effect of a novel inactivated intranasal (IN) trivalent whole influenza ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:2000-05-01 00:00:00
abstract::Noroviruses (called formerly "Norwalk-like viruses") cause food-borne gastroenteritis outbreaks. These outbreaks were thought to be related to shellfish consumption, although non-shellfish related outbreaks also occurred frequently in Japan. To clarify the epidemiology of Norovirus outbreaks, 435 stool samples were co...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20455
更新日期:2005-10-01 00:00:00
abstract::Rapid PCR-based influenza tests are increasingly used as point-of-care diagnostics in hospitals and clinics. To our knowledge, no prior studies have described clinical outcomes with implementation of rapid PCR-based influenza tests in hospitalized adult inpatients. Electronic medical records were used to assess differ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24279
更新日期:2015-12-01 00:00:00
abstract::Sex differences in the predictors for hepatocellular carcinoma (HCC) development after direct-acting antiviral (DAA) therapy was investigated. DAA therapy was given to 1438 (663 male, 775 female) patients. Sex differences in the HCC development rate and the factors contributing to HCC development after DAA therapy wer...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25984
更新日期:2020-05-06 00:00:00
abstract::Natural history of anal intraepithelial neoplasia and anal cancer is not fully understood. Factors associated with cytological abnormalities and predictors of progression to high-grade anal intraepithelial neoplasia still deserve investigation. The aim of this cross-sectional study was to assess the prevalence of HPV ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23831
更新日期:2014-03-01 00:00:00
abstract::In the ongoing coronavirus disease 2019 (COVID-19) pandemic, one potential cause of concern is that some discharged COVID-19 patients are testing positive again for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA. To better understand what is happening and to provide public health policy planners and ...
journal_title:Journal of medical virology
pub_type: 杂志文章,评审
doi:10.1002/jmv.26250
更新日期:2020-07-01 00:00:00
abstract::A procedure is described for the detection of hepatitis C virus (HCV) RNA in blood by means of the polymerase chain reaction (PCR) in which the reverse transcription step and two rounds of amplification are carried out in a single tube. This results in fewer manipulations, reduced risk of contamination, and economy of...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890380312
更新日期:1992-11-01 00:00:00
abstract::Hepatitis A infections still represent a major global health concern. During the past years, a transition pattern of the hepatitis A epidemiology was noted in many parts of the world. In Tunisia, there is not a recent survey on age-specific hepatitis A virus seroprevalence. This study aimed to investigate the seroprev...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26563
更新日期:2020-09-28 00:00:00
abstract::Investigation of varicella-zoster virus (VZV) DNA in 20 healthy children with varicella vaccination was carried out by using the polymerase chain reaction (PCR) and nested double PCR. Samples of peripheral blood mononuclear cells (PBMC) and throat swabs were simultaneously collected 3 times (before, 1 week, and 4 week...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890420110
更新日期:1994-01-01 00:00:00
abstract::Although risk factors for cirrhosis in chronic hepatitis C virus (HCV) infection have been identified, the role of HCV-genotype 3 remains controversial, and limited data are available in drug users. The aim of the study was to assess risk factors for severe liver disease (cirrhosis/hepatocellular carcinoma) in HCV-inf...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21850
更新日期:2010-10-01 00:00:00
abstract::During 2002, an upsurge in frequency of hepatitis A outbreaks among injecting drug users was observed in England and Wales. As lack of risk factor information and the high mobility of the cases made linkage of outbreaks difficult, the relationship of nucleotide sequences in the VP1/2PA junction of the hepatitis A viru...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21207
更新日期:2008-07-01 00:00:00
abstract::The Balkan Peninsula and Bulgaria in particular, is a well-known endemic region for Crimean-Congo hemorrhagic fever (CCHF). This study describes the prevalence of Crimean-Congo hemorrhagic fever virus (CCHFV) among tick populations from areas, previously recognized with emerging cases of CCHF disease in humans. These ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23214
更新日期:2012-04-01 00:00:00
abstract::The TT virus (TTV) was isolated recently from the serum of a patient with post-transfusion hepatitis. TTV infection is widespread in the general population, and its prevalence increases continuously with age. The pathogenic role of TTV in liver disease remains controversial, and the source of transmission is still unc...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20204
更新日期:2004-11-01 00:00:00
abstract::A total of 41 stool rotavirus specimens collected from children with acute diarrhea at four different locations in Akita Prefecture, Japan, during the peak of the winter diarrhea epidemic in 1988 were analyzed by polyacrylamide gel electrophoresis of viral RNA in conjunction with subgrouping assay. We found that a sin...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890280311
更新日期:1989-07-01 00:00:00
abstract::This study aimed to examine the application of hepatitis B surface antigen (HBsAg) confirmatory testing when diagnosing hepatitis B infection among young persons in Taiwan with a low prevalence rate of hepatitis B infection. HBsAg status, the presence of antibodies against HBsAg (anti-HBs), and the presence of antibod...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.22127
更新日期:2011-09-01 00:00:00
abstract::Five temperature-sensitive mutants (tsm9, tsm13, tsm20, tsm22, tsm30) of murine cytomegalovirus have been shown previously not to produce infectious virus in mice. In the present study, the stage at which these mutants are blocked in their replication in vitro was examined by transcriptional analysis of 4 temporally r...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/1096-9071(200010)62:2<127::aid-jmv2>3.0.co
更新日期:2000-10-01 00:00:00
abstract::Enterovirus D68 (EV-D68) is an emergent viral pathogen associated with mild to severe respiratory infections. In this study, we describe respiratory infections associated with EV-D68 in Beijing over a 4 year period. Total nucleic acid was extracted from 7,945 clinical specimens collected between January 5, 2011 and Ju...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24505
更新日期:2016-09-01 00:00:00
abstract::Transmission of West Nile virus (WNV) from asymptomatic donors has been reported during blood transfusions and organ transplants in humans. In this work, we aimed to investigate the presence of WNV antibody and WNV RNA in blood donors to evaluate the sero-prevalence of WNV and risk for WNV transmission. One hundred an...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24682
更新日期:2017-08-01 00:00:00
abstract::Varicella zoster-virus (VZV) is a humanpathogenic alpha-Herpesvirus that causes chickenpox after primary infection. The virus spreads by aerosol or direct contact with infectious vesical fluids, it enters the body via the respiratory tract. In a first viremic stage it replicates in local lymph nodes, followed by a sec...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10323
更新日期:2003-01-01 00:00:00
abstract::The predictive value of two methods for measuring HIV RNA concentration in plasma was assessed in relation to CD4 lymphocyte counts during the asymptomatic period of infection. The design was a retrospective longitudinal case-control study for a mean period of 60 months involving 20 asymptomatic patients included in t...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::Patients attending the genitourinary medicine clinic at Watford General Hospital, UK, were examined for clinical signs of genital herpes infection. Genital swabs were taken from 194 patients (126 female, 68 male) who presented with genital ulceration or symptoms which were suggestive of genital herpes infection. Swabs...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:1998-06-01 00:00:00
abstract::To assess the prevalence of HPV infection in the genital tracts of women with normal PAP smears, a random series of 109 women was reexamined using colposcopy, a further PAP smear, and punch biopsies taken from the cervix (in 33 cases), vagina (212 cases), and anus (20 cases). The biopsy material was examined using rou...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890310404
更新日期:1990-08-01 00:00:00
abstract::In order to demonstrate the viral specificity of IgM-containing immune complexes (IgM-CIC) detected by a C1q assay in renal allograft recipients developing a CMV infection, a technique is described allowing: 1) the dissociation of IgM-CIC by action of an acid buffer, and 2) the characterization of the viral specificit...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890330312
更新日期:1991-03-01 00:00:00