Abstract:
:A common receptor for coxsackie B virus and adenovirus has been described recently in cells of human and murine origin. Since the established cell line A-549 is suitable for adenoviruses, the potential use of A-549 cells for the isolation of coxsackie B viruses from clinical samples was investigated. All throat swabs sent to the laboratory between April 1998 and June 1999 were inoculated onto monolayers of MRC-5 and A-549 cells in tubes, and the enterovirus isolates obtained were typed. From April to June 1999, A-549 cells were compared prospectively to Buffalo green monkey (BGM) cells, considered as the most susceptible cell line for isolating coxsackie B viruses. Fifty-six out of 171 enterovirus isolates (33%) displayed a cytopathic effect (CPE) in the A-549 monolayer only, 48 isolates (28%) in the MRC-5 monolayer only, and 67 isolates (39%) in both cell lines. Most isolates that showed CPE in A-549 cells only (48 out of 56, 86%) were coxsackie B viruses, belonging to four different serotypes (B1, B2, B4, and B6). When BGM and A-549 cells were inoculated in parallel, both recovered the same number of coxsackie B isolates (n = 20), and the CPE was noted on approximately the same day. In conclusion, growth in A-549 but not MRC-5 cells identified coxsackie B viruses in most cases. A-549 was comparable to BGM for primary isolation of coxsackie B viruses.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Otero JR,Folgueira L,Trallero G,Prieto C,Maldonado S,Babiano MJ,Martinez-Alonso Ikeywords:
subject
Has Abstractpub_date
2001-11-01 00:00:00pages
534-6issue
3eissn
0146-6615issn
1096-9071pii
10.1002/jmv.2069journal_volume
65pub_type
杂志文章abstract::The relationships between host immune factors and HIV-1 disease progression are still in dispute. The RANTES SNPs exhibit distinct ethnic distribution and are associated with different effects on the course of HIV infection. Therefore, impact of RANTES gene polymorphism on HIV-1 transmission and progression needs to b...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21201
更新日期:2008-07-01 00:00:00
abstract::Among 39,656 voluntary blood donors in Okinawa Prefecture, Japan, 115 (0.29%) were repeatedly reactive for antibody to hepatitis C virus (anti-HCV) by second generation (2nd-gen) passive hemagglutination assay (PHA). Positive serum samples were tested for anti-HCV using three different enzyme immunosorbent assays (ELI...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890460408
更新日期:1995-08-01 00:00:00
abstract::The quantification of human immunodeficiency virus type 1 (HIV-1) RNA or hepatitis C virus (HCV) RNA has been facilitated by adapting a spin column procedure for sample preparation and the use of chemiluminescent detection of polymerase chain reaction (PCR) products in microtiter plate format. All materials were comme...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract::SARS-CoV-2 infection is causing a pandemic disease that is reflected in challenging public health problems worldwide. Human leukocyte antigen (HLA)-based epitope prediction and its association with disease outcomes provide an important base for treatment design. A bioinformatic prediction of T cell epitopes and their ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26561
更新日期:2020-09-28 00:00:00
abstract::Recently a conventional method for the laboratory diagnosis of human cytomegalovirus (HCMV) infection was improved by using centrifugation culture to enhance viral adsorption and by detecting HCMV early antigen and DNA. Comparison of the sensitivity of three rapid methods using commercial diagnostic reagents for the d...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890300204
更新日期:1990-02-01 00:00:00
abstract::The burden of herpes zoster may be related to patients' immunity, although this has not been studied extensively. This hypothesis was tested in a matched case-control study of patients with herpes zoster who sought treatment at one of seven university hospitals in Korea from January 1, 2007, to December 31, 2010. Pati...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23830
更新日期:2014-03-01 00:00:00
abstract::Hepatitis C virus (HCV) particles can be classified into two major fractions according to their floating density in serum. However, the genomic heterogeneity of each fraction and the relationship between this viral characteristic and interferon (IFN) response in patients with chronic hepatitis are not known. In this s...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:1998-05-01 00:00:00
abstract::Human herpesvirus 6 (HHV6) is a beta-herpesvirus capable of infecting several cell types from different origins. HHV6 is the etiological agent of exantem subitum and has been associated with several diseases, all characterized by an inflammatory response triggered by chemokines. We show that strain U1102 of HHV6 is ab...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10416
更新日期:2003-07-01 00:00:00
abstract::Varicella-zoster virus (VZV)-specific cell-mediated immunity (CMI) is critical for preventing and controlling the onset of herpes zoster (HZ). To assess VZV CMI, an interferon-γ (IFN-γ) enzyme-linked immunosorbent assay (ELISA) was validated by examining the influence of VZV-specific antigen content, incubation time, ...
journal_title:Journal of medical virology
pub_type: 杂志文章,随机对照试验
doi:10.1002/jmv.25391
更新日期:2019-05-01 00:00:00
abstract::Increased expression of inducible nitric oxide synthase (iNOS) has been found in inflammatory myocardial disease and increased production of nitric oxide (NO) has both an inhibitory effect on virus replication and a cytotoxic effect on host cells. To investigate the relationship between severity of enteroviral myocard...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1033
更新日期:2001-06-01 00:00:00
abstract::HIV-1 infections due to non-B subtypes are increasing rapidly in number and spreading across Europe. The genetic nature of HIV-1 non-B variants containing subtype G sequences at the protease (PR)-coding region are described from 48 unrelated subjects living in Spain. Phylogenetic analyses of the HIV-1 reverse transcri...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20280
更新日期:2005-03-01 00:00:00
abstract::Human papillomavirus (HPV) RNA levels may be a more sensitive early indicator of predisposition to carcinogenesis than DNA levels. We evaluated whether levels of HPV-16 and HPV-18 DNA and messenger RNA (mRNA) in newly detected infections are associated with cervical lesion development. Female university students were ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21450
更新日期:2009-04-01 00:00:00
abstract::Mitochondrial DNA (mtDNA) of peripheral blood mononuclear cells (PBMCs) collected from Human immunodeficiency virus 1 (HIV-1)-infected patients and healthy controls were measured longitudinally using real-time polymerase chain reaction to evaluate the effects of antiretroviral agents on mtDNA synthesis in vivo and to ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10423
更新日期:2003-08-01 00:00:00
abstract::An attempt was made to identify factors influencing the cumulative probability of an increased alanine aminotransferase (ALT) level and hepatocarcinogenesis in hepatitis C patients with a normal ALT level initially. A total of 398 consecutive patients with a normal ALT level initially for 6 months or more and follow-u...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21342
更新日期:2009-03-01 00:00:00
abstract::Dengue virus (DV) infection can result in either a mild febrile illness known as dengue fever (DF) or a life-threatening disease called dengue hemorrhagic fever (DHF). DHF is more prevalent in patients undergoing secondary DV infection. This observation has led to the hypothesis that DHF may be the result of immune re...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.2037
更新日期:2001-10-01 00:00:00
abstract::Algeria is the largest country of Africa, peopled with populations living a range of traditional/rural and modern/urban lifestyles. The variations of prevalence of chronic active hepatitis care poorly known on the Algerian territory. We conducted a retrospective survey on all patients (n = 998) referred to our institu...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24491
更新日期:2016-08-01 00:00:00
abstract::Hepatitis E virus (HEV) is a feco-orally transmitted pathogen and one of the most common cause of acute hepatitis worldwide. Recent studies in developed countries suggested that a direct human-to-human contact such as for sexually transmitted diseases may play a significant role in the HEV spread. The aim of this stud...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25052
更新日期:2018-08-01 00:00:00
abstract::The TT virus (TTV) was isolated recently from the serum of a patient with post-transfusion hepatitis. TTV infection is widespread in the general population, and its prevalence increases continuously with age. The pathogenic role of TTV in liver disease remains controversial, and the source of transmission is still unc...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20204
更新日期:2004-11-01 00:00:00
abstract:BACKGROUND AND AIM:The hepatitis B virus (HBV) prevalence study performed in 2003 in Belgium is believed to be underestimating HBV prevalence due to underrepresentation of the non-Belgian population. Therefore, we assessed the prevalence and risk factors of HBV infection in a multi-ethnic region situated in Middle-Limb...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25457
更新日期:2019-08-01 00:00:00
abstract::A solution of pooled cryo-precipitate for preparing factor VIII (F VIII) by the solvent/detergent method was contaminated experimentally with parvovirus B19-positive plasma to evaluate virus reduction achieved by the final steps of the F VIII production process. Virus reduction was at least 2 logs of the total amount ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890350107
更新日期:1991-09-01 00:00:00
abstract::The human respiratory syncytial virus (HRSV) is the most important cause of admission to hospital for acute lower respiratory tract infections in infants and young children worldwide. Only few studies have investigated the molecular evolution of HRSV, and none has been conduct ed in Italy. The genetic diversity of the...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21012
更新日期:2007-12-01 00:00:00
abstract::Previous studies demonstrated that in vitro infection with influenza A viruses altered several functions of human monocytes-macrophages but did not detectably alter functions of human lymphocytes. However, both types of cells were infected, as determined by production and surface expression of viral antigens. In the c...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890270302
更新日期:1989-03-01 00:00:00
abstract::Virus fragments homologous to TTV were detected previously from an enterically transmitted outbreak of non-A-E hepatitis [Luo et al., 1999]. To test the susceptibility of the Rhesus monkey to this virus and to establish its transmission routes, 6 Rhesus monkeys were inoculated, 3 orally and another 3 intravenously. Th...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(200005)61:1<159::aid-jmv26
更新日期:2000-05-01 00:00:00
abstract::Varicella zoster virus (VZV) causes varicella (chickenpox), after which virus becomes latent in ganglia along the entire neuraxis. Virus reactivation produces zoster (shingles). Infectious VZV is found in vesicles of patients with zoster and varicella, but virus shed in the absence of disease has not been documented. ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21173
更新日期:2008-06-01 00:00:00
abstract::Hepatitis E virus (HEV), the sole member of the genus Hepevirus in the family of Hepeviridae, is the major cause of several outbreaks of waterborne hepatitis in tropical and subtropical countries and of sporadic cases of viral hepatitis in endemic and industrialized countries. Transmission of HEV occurs predominantly ...
journal_title:Journal of medical virology
pub_type: 杂志文章,评审
doi:10.1002/jmv.21116
更新日期:2008-04-01 00:00:00
abstract::As the coronavirus disease 2019 pandemic keep tackling global public health systems worldwide. The severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) genome keeps mutating. In that regard, the recent emergence of the B.1.1.7 lineage in the UK has called the attention of global authorities. One point of concer...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26823
更新日期:2021-01-28 00:00:00
abstract::In order to evaluate the true immune status and the effect of revaccination on a young adult population, we collected serum samples from 289 military recruits who were vaccinated during an outbreak in 1991. Most vaccinees, age 18-25 years, had apparently been immunized once before as infants. Sera collected just prior...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(SICI)1096-9071(199611)50:3<249::AID-JMV7>
更新日期:1996-11-01 00:00:00
abstract::The S RNA genome segment of hantaviruses carried by Arvicolinae and Sigmodontinae rodents encodes the nucleocapsid (N) protein and has an overlapping (+1) open reading frame (ORF) for a putative nonstructural protein (NSs). The aim of this study was to determine whether the ORF is functional. A protein corresponding t...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20948
更新日期:2007-10-01 00:00:00
abstract::Human T-cell lymphotropic virus 1 (HTLV-1) is associated with two progressive diseases: HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) and adult T-cell leukemia/lymphoma (ATLL). Although HTLV-1 proviral load (PVL) has been introduced as a risk factor for these diseases' progression, it is not suff...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24721
更新日期:2017-06-01 00:00:00
abstract:BACKGROUND AND AIM:A meta-analysis was performed to identify COVID-19 patients presenting with gastrointestinal (GI) symptoms during the first and second pandemic waves and investigate their association with the disease outcomes. METHODS:A systematic search in PubMed, Scopus, Web of Science, ScienceDirect, and EMBASE ...
journal_title:Journal of medical virology
pub_type: 杂志文章,评审
doi:10.1002/jmv.26836
更新日期:2021-02-02 00:00:00