Abstract:
:Enteroviruses are responsible for hand, foot, and mouth disease, and have caused many deaths in China during recent years. But the natural history of enterovirus infection in children, especially asymptomatic children, is not yet clear. From April 2011 to May 2012, 505 stool and throat swab samples of children attending outpatients clinics in two hospitals were collected weekly to test for Enterovirus 71, Coxsackievirus A16, and other enterovirus nucleic acids by real-time RT-PCR. Two hundred sixty-four patients were enterovirus positive, the positive rate was 52.3%, 27.5% (22/80) in children without a rash and 56.9% (242/425) in children with a rash. Coxsackievirus A16 positive rate of male (24%, 61/254) was higher than that of female (15.2%, 26/171) (χ(2) = 4.87, P = 0.027). The highest positive rate of enterovirus infection was 63.5% in the 2-year-old age group. Comparing children with and without a rash, within the same age groups, no statistical difference was found (P > 0.05). The seasonal distribution of Enterovirus 71 had only one peak in May, but Coxsackievirus A16 had two peaks in April and October. In patients with a rash, the frequency of Enterovirus 71 was relatively high before July, and then that of Coxsackievirus A16 increased gradually. In the case of Enterovirus 71 and Coxsackievirus A16, stool specimens had a higher positive rate than throat swab specimens' (χ(2) = 3.88, P = 0.05; χ(2) = 15.13, P < 0.001). Enterovirus infection was more frequent in males 2-3 year-old children, with the implicated virus varying by season. Targeted prevention and control measures should be carried out.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Cai J,Lv H,Lin J,Chen Z,Fang C,Han Jdoi
10.1002/jmv.23884subject
Has Abstractpub_date
2014-09-01 00:00:00pages
1602-8issue
9eissn
0146-6615issn
1096-9071journal_volume
86pub_type
杂志文章abstract::Many of the small round human fecal viruses implicated in outbreaks of nonbacterial gastroenteritis have been collected together and examined under the electron microscope. Negatively stained preparations without the addition of antibody were used so that the surface morphology of the virus particles remained clearly ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890090403
更新日期:1982-01-01 00:00:00
abstract::Although risk factors for cirrhosis in chronic hepatitis C virus (HCV) infection have been identified, the role of HCV-genotype 3 remains controversial, and limited data are available in drug users. The aim of the study was to assess risk factors for severe liver disease (cirrhosis/hepatocellular carcinoma) in HCV-inf...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21850
更新日期:2010-10-01 00:00:00
abstract::The predictive value of two methods for measuring HIV RNA concentration in plasma was assessed in relation to CD4 lymphocyte counts during the asymptomatic period of infection. The design was a retrospective longitudinal case-control study for a mean period of 60 months involving 20 asymptomatic patients included in t...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::Some aspects of rotavirus humoral immunity were assessed on the basis of distinguishing serotype-specific specificities (VP4/VP7) by using rotavirus reassortants, human and animal strains in neutralization assays in serum samples obtained during the acute phase, and 1, 6 and 12 months after primary natural infection. ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890470418
更新日期:1995-12-01 00:00:00
abstract::The importance of hepatitis B virus (HBV) genotypes for disease progression and response to interferon-alpha-based treatment is well established. While almost all patients in the Mediterranean area are infected with HBV genotype D, HBV genotype A is dominant in Northern Europe. However, the distribution of HBV genotyp...
journal_title:Journal of medical virology
pub_type: 杂志文章,多中心研究
doi:10.1002/jmv.21294
更新日期:2008-10-01 00:00:00
abstract::Hepatitis E virus (HEV), a positive-strand RNA agent, has been associated with enterically transmitted non-A, non-B hepatitis in Asia, Africa, and Mexico. To evaluate the role of HEV in an outbreak of hepatitis in Pakistan, we used immune electron microscopy to detect 1) antibody to HEV, for evidence of infection, and...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890360205
更新日期:1992-02-01 00:00:00
abstract::To determine the influence of ageing per se as well as of priming histories on the antibody response to influenza vaccination, haemagglutination inhibition (HI), ELISA IgG, IgA, IgM and neutralizing antibody titres were studied in 43 healthy young subjects (mean age 23 years) and 55 healthy elderly people (mean age 79...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(199805)55:1<82::aid-jmv13>
更新日期:1998-05-01 00:00:00
abstract::Knowledge of the antigenic diversity of rotaviruses circulating in a region should be acquired before introducing a rotavirus vaccine. In a collection of 151 rotavirus-positive samples from Mendoza, Argentina, strain diversity was evaluated utilizing G-typing monoclonal antibodies (MAbs), reverse-transcriptase-polymer...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:2000-08-01 00:00:00
abstract::Major differences exist between HIV-1 and HIV-2 in terms of epidemiology, pathogenicity, sensitivity to antiretrovirals. Determining the type of HIV infecting a patient is essential for management. The aim of this study was to evaluate the ability of simple/rapid tests to differentiate between HIV-1 and/or HIV-2 infec...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24282
更新日期:2015-12-01 00:00:00
abstract::In eight patients with biopsy-confirmed chronic active hepatitis (CAH), hepatitis B e antigen (HBeAg) levels and DNA polymerase (DNA-P) activity were assayed three times a week for six weeks and once a week for another six weeks. HBeAg levels were rather constant, whereas DNA-P activity fluctuated. No correlation was ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/1096-9071(1980)5:4<295::aid-jmv1890050405>
更新日期:1980-01-01 00:00:00
abstract::The ability of the total hepatitis C virus (HCV) core antigen assay was evaluated for monitoring the therapeutic responses of HCV-infected patients treated with interferon. The ability to detect and quantitate an independent structural protein component of HCV, in the presence of circulating antibodies, makes this ass...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10355
更新日期:2003-05-01 00:00:00
abstract::Using the RT-PCR with primers that anneal to the 5' and the 3' extremities of the genome segments of bunyaviruses and internal primers that anneal to the S segment of Simbu serogroup viruses in a nested PCR it was possible to amplify the Oropouche virus (ORO) genome from the sera of three patients. These results show ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.2122
更新日期:2002-01-01 00:00:00
abstract::The objectives of this study were to evaluate the reliability of herpes simplex virus (HSV) PCR testing in cerebrospinal fluid (CSF) for the detection of herpes simplex encephalitis. This was done by examining retrospectively the clinical follow-up of a large group of patients tested routinely by HSV-PCR. In addition,...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1082
更新日期:2001-08-01 00:00:00
abstract::Here, we studied the role of nitric oxide (NO) production during the first steps of the respiratory infection of BALB/c mice with herpes simplex virus type 1 (HSV-1), strain F. Nitric oxide synthase II (NOS-II) mRNA and protein were detected by reverse transcription (RT)-PCR and dot blot, respectively in samples of lu...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20093
更新日期:2004-06-01 00:00:00
abstract::Hepatitis E is endemic in India. It was recently noted that although all the Indian human hepatitis E virus (HEV) isolates (1976-2001) were placed in genotype I, the swine HEV recovered from western India (2000) belonged to genotype IV. This was in contrast to reports from the United States and Taiwan wherein both hum...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10301
更新日期:2003-03-01 00:00:00
abstract::KI is a novel polyomavirus identified in the respiratory secretions of children with acute respiratory symptoms. Whether this reflects a causal role of the virus in the human respiratory disease remains to be established. To investigate the presence of KIV in the respiratory tissue, we examined 20 fresh lung cancer sp...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21417
更新日期:2009-03-01 00:00:00
abstract::The presence of antibody to hepatitis A virus (anti-HAV) in 60% of procured owl monkeys (Aotus trivirgatus) held within the United States prompted a study of recently captured A trivirgatus in Panama. Only 2 of 145 newly captured monkeys, but all of 35 A trivirgatus held within a colony for over 100 days, were found t...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890100105
更新日期:1982-01-01 00:00:00
abstract::The human papillomavirus (HPV) 16 genome has been studied extensively, although no study has focused on the E1 gene that is implicated in viral DNA replication. After analyzing the E1 region of HPV 16 genomes in 429 cervical samples, 11.2% were found to contain a 63 nucleotides duplication in this region. Sequence ana...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21304
更新日期:2008-12-01 00:00:00
abstract::HIV excretion patterns and specific antibody responses were evaluated in blood, semen, female genital secretions, saliva, and crevicular fluid. Samples were examined for infectious virus, viral antigens, viral nucleic acid, HIV specific IgG, IgA, anti-nef, and anti-p24. Viral load in peripheral blood appeared to incre...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890310409
更新日期:1990-08-01 00:00:00
abstract::The epidemiology, transmission and clinical symptoms of human parechoviruses [HPeV, classified earlier as enteroviruses; echovirus 22 (HPeV1) and echovirus 23 (HPeV2)] remain poorly characterized. Enteroviruses and one parechovirus species, the Ljungan virus, have been associated with type 1 diabetes in humans and rod...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20831
更新日期:2007-04-01 00:00:00
abstract::A novel real-time PCR assay system was developed to quantify the cytomegalovirus (CMV) genome load. The real-time PCR assay could detect from 6 to over 10(6) copies of CMV-DNA with a wide linear range. The virus load of immunocompromised patients with symptomatic CMV infections was quantified and compared to that of a...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(200004)60:4<455::aid-jmv14
更新日期:2000-04-01 00:00:00
abstract::Parechoviruses are emerging pathogens of humans often affecting the pediatric age group, with a growing line of evidence implicating them as agents of a broad spectrum of clinical syndromes in adults. However, because many clinicians are not familiar with the manifestation of the infections, they are not included in t...
journal_title:Journal of medical virology
pub_type: 杂志文章,评审
doi:10.1002/jmv.26395
更新日期:2020-08-06 00:00:00
abstract::The ability of a variety of epithelial, embryonal, placental, and neuronal cells to express the CD4 antigen and to be infected by human immunodeficiency virus 1 (HIV-1) was examined. Only two (IMR-32 and HeLa-T4) expressed CD4 detectable by indirect immunofluorescence, and both were infectable by HIV-1. Two others, a ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890350308
更新日期:1991-11-01 00:00:00
abstract::We developed approaches using antisense RNA to inhibit hepatitis C virus (HCV) RNA translation and HCV core protein expression. An HCV genotype 1b cDNA comprising nt 1-1321 or a fusion construct consisting of HCV (nt 1-584) and luciferase cDNAs were inserted downstream of T7 and CMV promoter sequences and used to gene...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:1999-03-01 00:00:00
abstract::Distinct human papillomavirus (HPV) 18 variants are thought to differ in oncogenic potential and geographic distribution. As such, understanding the regional variants of HPV 18 would be of great importance for evolutionary, epidemiological, and biological analysis. In this regard, the sequence variations of E6 gene we...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26283
更新日期:2020-07-09 00:00:00
abstract::Does anal HPV viral load explain the difference in anal HPV persistence between HIV-negative and -positive men who have sex with men (MSM)? MSM ≥18 years were recruited in Amsterdam, the Netherlands, in 2010-2011. Anal self-swabs were collected every 6 months and genotyped (SPF10 -PCR-DEIA-LIPA25 -system). HPV16 and H...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24898
更新日期:2018-01-01 00:00:00
abstract::Therapy failure due to drug resistance development is a common phenomenon in HIV-infected patients. However, when the drug pressure leads to the earliest selection of drug-resistant HIV-1 populations is still unclear. In this study, the extent to which selection of the HIV-1 reverse transcriptase M184I/V mutations occ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21363
更新日期:2009-01-01 00:00:00
abstract::To elucidate the role of host immune status in the evolution and complexity of hepatitis C virus (HCV) quasispecies, three chronic HCV-infected patients who underwent bone marrow transplantation (BMT) were studied. The three transplanted patients' sera were sampled at pre-BMT, 3 months after BMT, and 12 months after B...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(199906)58:2<132::aid-jmv6>
更新日期:1999-06-01 00:00:00
abstract::The incidence of perinatal transmission of hepatitis B virus (HBV) depends on the HBeAg/anti-HBe status of the mother. While children of HBeAg-positive mothers have a 90% probability of acquiring a chronic hepatitis B virus carrier state, babies of anti-HBe-positive mothers are more likely to develop fulminant hepatit...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890470408
更新日期:1995-12-01 00:00:00
abstract::WU and KI polyomaviruses (WUPyV, KIPyV) have been detected in respiratory, blood, stool, and lymphoid tissue, but not in urine samples. PCR based detection revealed higher frequency in immunocompromised individuals. In this study the prevalence of WUPyV and KIPyV was analyzed in respiratory, urine, and blood samples f...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.22083
更新日期:2011-07-01 00:00:00